Production and purification of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) from high cell density cultures of Pichia pastoris

Bioprocess and Biosystems Engineering

Volumn 30, Issue 5, 2007, Pages 305-312

  Bhatacharya, Palash ^a   Pandey, Gaurav ^a   Mukherjee, Krishna Jyoti ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

GM CSF; High cell density; Pichia pastoris; Purification

Indexed keywords

CELL CULTURE; CELL GROWTH; ELECTROPHORESIS; PROTEINS; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; YEAST;

CATION EXCHANGE CHROMATOGRAPHY; CELL DENSITY CULTURES; GRANULOCYTES; HEMATOPOIETIC GROWTH FACTORS; PICHIA PASTORIS; THERAPEUTIC AGENTS;

MACROPHAGES;

RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; CATION; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; RECOMBINANT PROTEIN;

ARTICLE; BIOMASS; CATION EXCHANGE; CYTOKINE PRODUCTION; DRUG SYNTHESIS; FUNGAL CELL CULTURE; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; METHYLOTROPHIC YEAST; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; BIOREACTOR; BIOTECHNOLOGY; CHEMISTRY; GENE VECTOR; HEMATOPOIETIC SYSTEM; HUMAN; ION EXCHANGE CHROMATOGRAPHY; METABOLISM; METHODOLOGY; PICHIA; PLASMID; PRENATAL DEVELOPMENT; PROMOTER REGION; REPRODUCIBILITY;

PICHIA PASTORIS;

BIOMASS; BIOREACTORS; BIOTECHNOLOGY; CATIONS; CHROMATOGRAPHY, ION EXCHANGE; GENETIC VECTORS; GLYCOSYLATION; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HEMATOPOIETIC SYSTEM; HUMANS; PICHIA; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 35748931483     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-007-0124-1     Document Type: Article

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