High yield production of a mutant Nippostrongylus brasiliensis acetylcholinesterase in Pichia pastoris and its purification

Biotechnology and Bioengineering

Volumn 93, Issue 5, 2006, Pages 1017-1022

  Richter, Sven ^a   Nieveler, Jens ^a   Schulze, Holger ^a   Bachmann, Till T ^a   Schmid, Rolf D ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Acetylcholinesterase; Biosensor; Fermentation; Nippostrongylus brasiliensis; Pichia pastoris; Purification

Indexed keywords

CELL CULTURE; CELLS; OXYGEN; PURIFICATION; YEAST;

ACETYLCHOLINESTERASE; EXPRESSION RATE; NIPPOSTRONGYLUS BRASILIENSIS; PICHIA PASTORIS;

ENZYMES;

ACETYLCHOLINESTERASE; DISSOLVED OXYGEN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CELL DENSITY; CROSSFLOW FILTRATION; ENZYME ACTIVITY; GEL FILTRATION; NIPPOSTRONGYLUS BRASILIENSIS; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; YEAST;

ACETYLCHOLINESTERASE; ANIMALS; BIOMASS; CATALYTIC DOMAIN; CHLORPYRIFOS; CHOLINESTERASE INHIBITORS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; METHANOL; MUTATION; NIPPOSTRONGYLUS; OXYGEN; PICHIA; RECOMBINANT PROTEINS; TIME FACTORS; TRANSFECTION;

NIPPOSTRONGYLUS BRASILIENSIS; PICHIA PASTORIS;

EID: 33646056991     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20705     Document Type: Article

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