Constitutive expression, purification and characterization of bovine prochymosin in Pichia pastoris GS115

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 5, 2012, Pages 2087-2093

  Jiang, X P ^a   Yin, M L ^a   Chen, P ^a   Yang, Q ^a  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Calf chymosin; Cheese preparation; Constitutive expression; Pichia pastoris GS115

Indexed keywords

AMMONIUM SULFATE; ANION EXCHANGE; BREAK DOWN; CATION EXCHANGE CHROMATOGRAPHY; CHYMOSIN; CONSTITUTIVE EXPRESSION; MILK COAGULATION; MILK-CLOTTING ACTIVITY; OPTIMAL ACTIVITY; PEPTIDE BONDS; PICHIA PASTORIS; PURIFIED ENZYME; SDS-PAGE; SINGLE BAND;

CASEIN; COAGULATION; ENZYMES; MAMMALS; MANGANESE; MANGANESE COMPOUNDS; POSITIVE IONS; PURIFICATION;

YEAST;

CASEIN; CHYMOSIN; ENZYME ACTIVATOR; ENZYME PRECURSOR; METAL; PROCHYMOSIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; CHEMISTRY; ENZYME STABILITY; GENE EXPRESSION; GENETICS; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MILK; MOLECULAR WEIGHT; PH; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; TEMPERATURE;

ANIMALS; CASEINS; CATTLE; CHEMICAL PRECIPITATION; CHROMATOGRAPHY, ION EXCHANGE; CHYMOSIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATORS; ENZYME PRECURSORS; ENZYME STABILITY; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; METALS; MILK; MOLECULAR WEIGHT; PICHIA; RECOMBINANT PROTEINS; TEMPERATURE;

BOVINAE; PICHIA PASTORIS;

EID: 84863407842     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-012-1012-7     Document Type: Article

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