Development of a new in vitro skin sensitization assay (Epidermal Sensitization Assay; EpiSensA) using reconstructed human epidermis

Toxicology in Vitro

Volumn 27, Issue 8, 2013, Pages 2213-2224

  Saito, Kazutoshi ^a   Nukada, Yuko ^a   Takenouchi, Osamu ^a   Miyazawa, Masaaki ^a   Sakaguchi, Hitoshi ^a   Nishiyama, Naohiro ^a  

^a KAO CORPORATION   (Japan)

Author keywords

Alternative method; Gene expression; Keratinocytes; Microarray analysis; Reconstructed human epidermis (RhE) model; Skin sensitization

Indexed keywords

1 FLUORO 2,4 DINITROBENZENE; ACTIVATING TRANSCRIPTION FACTOR 3; BENZALKONIUM CHLORIDE; COMPLEMENTARY DNA; HAPTEN; HEAT SHOCK PROTEIN; ISOEUGENOL; OXAZOLONE; PHENYLENEDIAMINE; PROTEIN DNAJ;

ARTICLE; CELL VIABILITY; DNA SYNTHESIS; EPIDERMIS; GENE EXPRESSION; HUMAN; IN VITRO STUDY; KERATINOCYTE; MICROARRAY ANALYSIS; MITOSIS; NUCLEOTIDE SEQUENCE; PREDICTION; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; SKIN SENSITIZATION; TISSUE CULTURE; UNINDEXED SEQUENCE; UPREGULATION;

1-FLUORO-2,4-DINITROBENZENE; 2,4-DINITROCHLOROBENZENE; 2-MBT; 2-MERCAPTOBENZOTHIASOLE; 4-NBB; 4-NITROBENZYL BROMIDE; ACD; ACETONE: OLIVE OIL=4:1; ACTIVATING TRANSCRIPTION FACTOR 3; ADH; ALCOHOL DEHYDROGENASE; ALLERGIC CONTACT DERMATITIS; ALTERNATIVE METHOD; AOO; ATF3; BENZALKONIUM CHLORIDE; BKC; CA; CINA; CINNAMIC ALCOHOL; CINNAMIC ALDEHYDE; CYP; CYTOCHROME P450; DATABASE FOR ANNOTATION, VISUALIZATION AND INTEGRATED DISCOVERY; DAVID; DCS; DENDRITIC CELLS; DNAJ (HSP40) HOMOLOG, SUBFAMILY B, MEMBER 4; DNAJB4; DNCB; DNFB; ECVAM; ENDOPLASMIC RETICULUM; EPIDERMAL SENSITIZATION ASSAY; EPISENSA; ER; EUROPEAN CENTRE FOR THE VALIDATION OF ALTERNATIVE METHODS; GCLM; GENE EXPRESSION; GENE ONTOLOGY; GLUTAMATE-CYSTEINE LIGASE, MODIFIER SUBUNIT; GLUTATHIONE; GO; GSH; HEAT SHOCK 105KDA/110KDA PROTEIN 1; HEAT SHOCK 70KDA PROTEIN 6; HSPA6; HSPH1; IEU; IL; INTERLEUKIN; ISOEUGENOL; KCS; KERATINOCYTES; LA; LACTIC ACID; LLNA; LOCAL LYMPH NODE ASSAY; MDBGN; METHYLDIBROMO GLUTARONITRILE; METHYLTHIAZOLYDIPHENYL-TETRAZOLIUM; MICROARRAY ANALYSIS; MTT; OXA; OXAZOLONE; P-PHENYLENEDIAMINE; PPD; REACTIVE OXYGEN SPECIES; RECONSTRUCTED HUMAN EPIDERMIS; RECONSTRUCTED HUMAN EPIDERMIS (RHE) MODEL; RHE; ROS; SA; SALICYLIC ACID; SKIN SENSITIZATION; SLS; SODIUM LAURYL SULFATE; TETRAMETHYLTHIURAM DISULFIDE; TMTD; TNF; TUMOR NECROSIS FACTOR;

ALLERGENS; ANIMAL TESTING ALTERNATIVES; BENZALKONIUM COMPOUNDS; DINITROFLUOROBENZENE; EPIDERMIS; GENE EXPRESSION PROFILING; HAPTENS; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OXAZOLONE; SKIN IRRITANCY TESTS;

EID: 84885758688     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2013.08.007     Document Type: Article

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