External and semi-internal controls for PCR amplification of homologous sequences in mixed templates

Journal of Microbiological Methods

Volumn 95, Issue 2, 2013, Pages 285-294

  Kalle, Elena ^a   Gulevich, Alexander ^b   Rensing, Christopher ^c  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b ALL RUSSIA RESEARCH INSTITUTE OF AGRICULTURAL BIOTECHNOLOGY   (Russian Federation)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Guidelines; Microbial diversity; PCR bias; PCR protocol; Polymerase; Quality assurance

Indexed keywords

DNA POLYMERASE; EXONUCLEASE;

ARTICLE; ARTIFACT REDUCTION; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

BOVINE SERUM ALBUMIN; BSA; CETYLTRIMETHYLAMMONIUM BROMIDE; CTAB; DENATURING GRADIENT GEL ELECTROPHORESIS; DENATURING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; DG-DGG; DG-DGGE; DGGE; DHPLC; DIMETHYL SULFOXIDE; DMSO; DOUBLE-GRADIENT DENATURING GRADIENT GEL; DOUBLE-GRADIENT DENATURING GRADIENT GEL ELECTROPHORESIS; GUIDELINES; MICROBIAL DIVERSITY; OPERATIONAL TAXONOMIC UNIT; OTU; PAAG; PCR; PCR BIAS; PCR PROTOCOL; POLYACRYLAMIDE GEL; POLYMERASE; POLYMERASE CHAIN REACTION; QUALITY ASSURANCE; TETRAMETHYLAMMONIUM CHLORIDE; TMA;

ACRYLIC RESINS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA, BACTERIAL; DNA, PLANT; POACEAE; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 84885459396     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.09.014     Document Type: Article

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