Practical Considerations in Design of Internal Amplification Controls for Diagnostic PCR Assays

Journal of Clinical Microbiology

Volumn 42, Issue 5, 2004, Pages 1863-1868

  Hoorfar, J ^a   Malorny, B ^b   Abdulmawjood, A ^c   Cook, N ^d   Wagner, M ^e   Fach, P ^f  

^a Danish Food and Veterinary Research Institute   (Denmark)

^b FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^c JUSTUS LIEBIG UNIVERSITY   (Germany)

^d Central Science Laboratory   (United Kingdom)

^e Institute for Milk Hygiene   (Austria)

^f National Research Agency   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; EDETIC ACID; PLASMID DNA;

AMPLICON; CLONING; DNA DEGRADATION; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GEL ELECTROPHORESIS; LABORATORY PERSONNEL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITY CONTROL; RECOMBINANT PLASMID; SALMONELLA ENTERICA; SHORT SURVEY; STANDARDIZATION;

BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; FOOD MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; QUALITY CONTROL;

ESCHERICHIA COLI; SALMONELLA; SALMONELLA ENTERICA;

EID: 2442562584     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.5.1863-1868.2004     Document Type: Short Survey

References (24)

1. Abdulmawjood, A., S. Roth, and M. Bülte. 2002. Two methods for construction of internal amplification controls for the detection of Escherichia coli 0157 by polymerase chain reaction. Mol. Cell. Probes 16:335-339.
2. Anonymous. 2002. Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. General method specific requirements (EN ISO 22174). International Organization for Standardization, Geneva, Switzerland.
3. Anonymous. 2002. Microbiology of food and animal feeding stuffs. Protocol for the validation of alternative methods (EN ISO 16140). European Committee for Standardization, AFNOR, Paris, France.
4. Belotserkovskii, B. P., and B. H. Johnston. 2003. Polypropylene tube surfaces may include denaturation and multimerisation of DNA. Science 271: 222-223.
5. Brightwell, G., M. Pearce, and D. Leslie. 1998. Development of internal controls for PCR detection of Bacillus anthracis. Mol. Cell. Probes 12:367-377.
6. Caplin, B. E., R. P. Rasmussen, P. S. Bernard, and C. T. Wittwer. 1999. LightCycler Hybridisation probes-the most direct way to monitor PCR amplification and mutation detection. Biochemica 1:5-8.
7. Courtney, B. C., M. M. Smith, and E. A. Henchal. 1999. Development of internal controls for probe-based nucleic acid diagnostic assays. Anal. Biochem. 270:249-256.
8. Cubero, J., J. van der Wolf, J. van Beckhoven, and M. M. Lopez. 2002. An internal control for the diagnosis of crown gall by PCR. J. Microbiol. Methods 51:387-392.
9. Fach, P., F. Dilasser, J. Grout, and J. Tache. 1999. Evaluation of a polymerase chain reaction-based test for detecting Salmonella spp. in food samples: Probelia Salmonella spp. J. Food Prot. 62:1387-1393.
10. Fach, P., S. Perelle, F. Dilasser, J. Grout, C. Dargaignaratz, L. Botella, J. M. Gourreau, F. Carlin, M. R. Popoff, and V. Broussolle. 2002. Detection by PCR-enzyme-linked immunosorbent assay of Clostridium botulinum in fish and environmental samples from a coastal area in northern France. Appl. Environ. Microbiol. 68:5870-5876.
11. Hoorfar, J., P. Ahrens, and P. Rådström. 2000. Automated 5′ nuclease PCR assay for identification of Salmonella enterica. J. Clin. Microbiol. 38:3429-3435.
12. Hoorfar, J., and N. Cook. 2003. Critical aspects of standardization of PCR. Methods Mol. Biol. 216:51-64.
13. Jensen, A. N., and J. Hoorfar. 2002. Optimal purification and sensitive quantification of DNA from fecal samples. J. Rapid Methods Automat. Microbiol. 10:231-244.
14. Jones, R. N., M. L. Neale, B. Beattie, D. Westmoreland, and J. D. Fox. 2000. Development and application of a PCR-based method including an internal control for diagnosis of congenital cytomegalovirus infection. J. Clin. Microbiol. 38:1-6.
15. Lübeck, P. S., P. Wolffs, S. L. W. On, P. Rådström, and J. Hoorfar. 2003. Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters: assay development and analytical validation. Appl. Environ. Microbiol. 69:5664-5669.
16. Malorny, B., C. Bunge, J. Hoorfar, and R. Helmuth. 2003. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl. Environ. Microbiol. 69:290-296.
17. Malorny, B., P. T. Tassios, P. Rådström, N. Cook, M. Wagner, and J. Hoorfar. 2003. Standardization of diagnostic PCR for the detection of food-borne pathogens. Int. J. Food Microbiol. 83:39-48.
18. Müller, F.-M., N. Schnitzler, O. Cloot, P. Kockelkorn, G. Haase, and Z. Li. 1998. The rationale and method for constructing internal control DNA used in pertussis polymerase chain reaction. Diagn. Microbiol. Infect. Dis. 31: 517-523.
19. Rådström, P., C. Löfström, M. Lövenklev, R. Knutsson, and P. Wolffs. 2003. Strategies for overcoming PCR inhibition, p. 149-161. In C. W. Diefenbach and G. S. Dveksler (ed.), PCR primer: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Reddy, N. R., B. N. Wilkie, and B. A. Mallard. 1996. Construction of an internal control to quantitate multiple porcine cytokine mRNAs by RTPCR. BioTechniques 21:868-875.
21. Rosenstraus, M., Z. Wang, S.-Y. Chang, D. DeBonville, and J. P. Spadoro. 1998. An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance. J. Clin. Microbiol. 36:191-197.
22. Sachadyn, P., and J. Kur. 1998. The construction and use of a PCR internal control. Mol. Cell. Probes 12:259-262.
23. Siebert, P. D., and J. W. Larrick. 1992. Competitive PCR. Nature 359:557-558.
24. Stöcher, M., V. Leb, and J. Berg. 2003. A convenient approach to the generation of multiple internal control DNA for a panel of real-time PCR assays. J. Virol. Methods 108:1-8.