Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment

FEMS Microbiology Letters

Volumn 221, Issue 2, 2003, Pages 299-304

  Hongoh, Yuichi ^a,d   Yuzawa, Hiroe ^a   Ohkuma, Moriya ^a,b   Kudo, Toshiaki ^a,c  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^c YOKOHAMA CITY UNIVERSITY   (Japan)

^d RIKEN   (Japan)

Author keywords

16S rDNA; Gut bacterium; Phylogenetic diversity; Rarefaction; Termite

Indexed keywords

RNA 16S; BACTERIAL DNA;

ARTICLE; BACTERIAL GENE; CLONE; ELECTROPHORESIS; GENE AMPLIFICATION; HOMOGENATE; ISOPTERA; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RNA GENE; SEQUENCE ALIGNMENT; SPECIES DIFFERENCE; TEMPERATURE; ANIMAL; BACTERIUM; CLASSIFICATION; CONFORMATION; DNA SEQUENCE; GENE LIBRARY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; SENSITIVITY AND SPECIFICITY; STANDARD;

BACTERIA (MICROORGANISMS); ISOPTERA; RETICULITERMES SPERATUS;

ANIMALS; BACTERIA; DNA, BACTERIAL; ENVIRONMENTAL MICROBIOLOGY; GENE LIBRARY; ISOPTERA; NUCLEIC ACID CONFORMATION; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

EID: 0142062399     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00218-0     Document Type: Article

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