Quantitative real-time PCR is not more sensitive than " conventional" PCR

Journal of Clinical Microbiology

Volumn 46, Issue 6, 2008, Pages 1897-1900

  Bastien, Patrick ^a   Procop, Gary W ^b   Reischl, Udo ^c  

^a UNIV MONTPELLIER   (France)

^b LERNER RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA POLYMERASE; MAGNESIUM ION;

BACTERIUM EXAMINATION; DIAGNOSTIC PROCEDURE; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FUNGAL EXAMINATION; GENE TARGETING; IMMUNOFLUORESCENCE TEST; INTERMETHOD COMPARISON; LEISHMANIA DONOVANI; MICROBIOLOGICAL EXAMINATION; MICROORGANISM; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NOTE; PARASITE EXAMINATION; PHYSICAL CHEMISTRY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEROLOGY; VIRUS DETECTION;

ANIMALS; DNA, BACTERIAL; DNA, PROTOZOAN; GENE DOSAGE; HUMANS; LEISHMANIA DONOVANI; LEISHMANIASIS, VISCERAL; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS; TUBERCULOSIS;

EID: 45149092382     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.02258-07     Document Type: Note

References (40)

1. Apfalter, P., U. Reischl, and M. R. Hammerschlag. 2005. In-house nucleic acid amplification assays in research: how much quality control is needed before one can rely upon the results? J. Clin. Microbiol. 43:5835-5841.
2. Bastien, P. 2002. Molecular diagnosis of toxoplasmosis. Trans. R. Soc. Trop. Med. Hyg. 96(Suppl. 1):S205-S215.
3. Beld, M. G. H. M., C. J. Birch, P. Cane, J. P. Clewley, E. Domingo, C. Escarmis, R. A. M. Fouchier, L. Jennings, I. D. Kay, O. Landt, S. Palladino, N. G. Papadopoulos, A. Petrich, M. W. Pfaffl, W. Rawlinson, U. Reischl, N. Saunders, C. Savolainen-Kopra, T. P. Sloots, Y. W. Tang, and P. C. Y. Woo. 2007. Experts roundtable: real-time PCR and microbiology, p. 357-443. In I. M. Mackay (ed.), Real-time PCR in microbiology: from diagnosis to characterization. Caister Academic Press, Norwich, United Kingdom.
4. Bensoussan, E., A. Nasereddin, F. Jonas, L. F. Schnur, and C. L. Jaffe. 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J. Clin. Microbiol. 44:1435-1439.
5. Bourlet, T., R. Levy, S. Laporte, S. Blachier, L. Bocket, G. Cassuto, L. Chollet, M. Leruez-Ville, A. Maertens, F. Mousnier, C. Pasquier, C. Payan, B. Pellegrin, E. Schvoerer, P. Zavadzki, J. Chouteau, G. Duverlie, J. Izopet, F. Lunel-Fabiani, J. M. Pawlotsky, N. Profizi, C. Rouzioux, F. Stoll-Keller, V. Thibault, P. Wattre, and B. Pozzetto. 2003. Multicenter quality control for the detection of hepatitis C virus RNA in seminal plasma specimens. J. Clin. Microbiol. 41:789-793.
6. Brenier-Pinchart, M. P., V. Morand-Bui, H. Fricker-Hidalgo, V. Equy, R. Marlu, and H. Pelloux. 2007. Adapting a conventional PCR assay for Toxoplasma gondii detection to real-time quantitative PCR including a competitive internal control. Parasite 14:149-154.
7. Buchbinder, S., R. Blatz, and A. C. Rodloff. 2003. Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii. Diagn. Microbiol. Infect. Dis. 45:269-271.
8. Burggraf, S., U. Reischl, N. Malik, M. Bollwein, L. Naumann, and B. Olgemoller. 2005. Comparison of an internally controlled, large-volume Light-Cycler assay for detection of Mycobacterium tuberculosis in clinical samples with the COBAS AMPLICOR assay. J. Clin. Microbiol. 43:1564-1569.
9. Chabbert, E., L. Lachaud, L. Crobu, and P. Bastien. 2004. Comparison of two widely used PCR primer systems for detection of Toxoplasma in amniotic fluid, blood and tissues. J. Clin. Microbiol. 42:1719-1722.
10. Chalker, V. J., H. Vaughan, P. Patel, A. Rossouw, H. Seyedzadeh, K. Gerrard, and V. L. A. James. 2005. External quality assessment for detection of Chlamydia trachomatis. J. Clin. Microbiol. 43:1341-1347.
11. Costa, J. M., C. Pautas, P. Ernault, F. Foulet, C. Cordonnier, and S. Bretagne. 2000. Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. J. Clin. Microbiol. 38:2929-2932.
12. Dagher, H., H. Donninger, P. Hutchinson, R. Ghildyal, and P. Bardin. 2004. Rhinovirus detection: comparison of real-time and conventional PCR. J. Virol. Methods 117:113-121.
13. Dworkin, L. L., T. M. Gibler, and R. N. Van Gelder. 2002. Real-time quantitative polymerase chain reaction diagnosis of infectious posterior uveitis. Arch. Ophthalmol. 120:1534-1539.
14. Edvinsson B., M. Lappalainen, B. Evengard, and ESCMID Study Group for Toxoplasmosis. 2006. Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Clin. Microbiol. Infect. 12:131-136.
15. Espy, M. J., J. R. Uhl, L. M. Sloan, S. P. Buckwalter, M. F. Jones, E. A. Vetter, J. D. Yao, N. L. Wengenack, J. E. Rosenblatt, F. R. Cockerill III, and T. F. Smith. 2006. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin. Microbiol. Rev. 19:165-256. (Erratum, 19: 595.)
16. Hafez, H. M., R. Hauck, D. Luschow, and L. McDougald. 2005. Comparison of the specificity and sensitivity of PCR, nested PCR, and real-time PCR for the diagnosis of histomoniasis. Avian Dis. 49:366-370.
17. Hassan, M. Q., A. Ghosh, S. S. Ghosh, M. Gupta, D Basu, K. K. Mallik, and S. Adhya. 1993. Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmania donovani: primers and probes for DNA diagnosis. Parasitology 107:509-517.
18. Hierl, T., U. Reischl, P. Lang, H. Hebart, M. Stark, P. Kyme, and I. B. Autenrieth. 2004. Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J. Med. Microbiol. 53:629-632.
19. Homan, W. L., M. Vercammen, J. De Braekeleer, and H. Verschueren. 2000. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int. J. Parasitol. 30:69-75.
20. Kaiser, K., A. M. Van Loon, H. Pelloux, J. Ferrandiz, S. Picot, P. Wallace, and F. Peyron. 2007. Multicenter proficiency study for detection of Toxoplasma gondii in amniotic fluid by nucleic acid amplification methods. Clin. Chim. Acta 375:99-103.
21. Katakura, K., S. Kawazu, T. Naya, K. Nagakura, M. Ito, M. Aikawa, J. Q. Qu, L. R. Guan, X. P. Zuo, J. J. Chai, K. P. Chang, and Y. Matsumoto. 1998. Diagnosis of kala-azar by nested PCR based on amplification of the Leishmania mini-exon gene. J. Clin. Microbiol. 36:2173-2177.
22. Lachaud, L., S. Marchergui-Hammami, E. Chabbert, J. Dereure, J. P. Dedet, and P. Bastien. 2002. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J. Clin. Microbiol. 40: 210-215.
23. Le Fichoux, Y., J. F. Quaranta, J. P. Aufeuvre, A. Lelievre, P. Marty, I. Suffia, D. Rousseau, and J. Kubar. 1999. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J. Clin. Microbiol. 37:1953-1957.
24. Li, H. J., J. S. Dummer, W. R. Estes, S. F. Meng, P. F. Wright, and Y. W. Tang. 2003. Cytomegalovirus loads measured by a quantitative real-time PCR for monitoring clinical intervention in transplant recipients. J. Clin. Microbiol. 41:187-191.
25. Lin, M. H., T. C. Chen, T. T. Kuo, C. C. Tseng, and C. P. Tseng. 2000. Real-time PCR for quantitative detection of Toxoplasma gondii. J. Clin. Microbiol. 38:4121-4125.
26. Loens, K., T. Beck, D. Ursi, S. Pattyn, H. Goossens, and M. Ieven. 2006. Two quality control exercises involving nucleic acid amplification methods for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and carried out 2 years apart (in 2002 and 2004). J. Clin. Microbiol. 44:899-908.
27. Lugert, R., C. Schettler, and U. Gross. 2006. Comparison of different protocols for DNA preparation and PCR for the detection of fungal pathogens in vitro. Mycoses 49:298-304.
28. Mackay, I. M. (ed.) 2007. Real-time PCR in microbiology: from diagnosis to characterization. Caister Academic Press, Norwich, United Kingdom.
29. Marfurt, J., I. Niederwieser, N. D. Makia, H. P. Beck, and I. Felger. 2003. Diagnostic genotyping of Old and New World Leishmania species by PCR-RFLP. Diagn. Microbiol. Infect. Dis. 46:115-124.
30. Mary, C., F. Faraut, L. Lascombe, and H. Dumon. 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J. Clin. Microbiol. 42:5249-5255.
31. Mortarino, M., A. Franceschi, F. Mancianti, C. Bazzocchi, C. Genchi, and C. Bandi. 2004. Quantitative PCR in the diagnosis of Leishmania. Parassitología 46:163-167. (In Italian with English summary.)
32. Nagaraj, T., J. P. Vasanth, A. Desai, A. Kamat, S. N. Madhusudana, and V. Ravi. 2006. Ante mortem diagnosis of human rabies using sativa samples: comparison of real time and conventional RT-PCR techniqies. J. Clin. Virol. 36:17-23.
33. Nagy, B., Z. Ban, A. Beke, G. R. Nagy, L. Lazar, C. Papp, E. Toth-Pal, and Z. Papp. 2006. Detection of Toxoplasma gondii from amniotic fluid, a comparison of four different molecular biological methods. Clin. Chim. Acta 368:131-137.
34. Pelloux, H., E. Guy, M. C. Angelici, H. Aspöck, M. H. Bessières, R. Blatz, M. Del Pezzo, V. Girault, R. Gratzl, M. Holberg-Petersen, J. Johnson, D. Krüger, M. Lappalainen, A. Naessens, and M. Olsson. 1998. A second European collaborative study on polymerase chain reaction for Toxoplasma gondii, involving 15 teams. FEMS Microbiol. Lett. 165:231-237.
35. Ravel, S., G. Cuny, J. Reynes, and F. Veas. 1995. A highly sensitive and rapid procedure for direct PCR detection of Leishmania infantum within human peripheral blood mononuclear cells. Acta Trop. 59:187-196.
36. Reischl, U., S. Bretagne, D. Kruger, P. Ernault, and J. M. Costa. 2003. Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect. Dis. 3:7.
37. Reithinger, R., J. C. Espinoza, O. Courtenay, and C. R. Davies. 2003. Evaluation of PCR as a diagnostic mass-screening tool to detect Leishmania (Viannia) spp. in domestic dogs (Canis familiaris). J. Clin. Microbiol. 41: 1486-1493.
38. Scotter, J. M., and S. T. Chambers. 2005. Comparison of galactomannan detection, PCR-enzyme-linked immunosorbent assay, and real-time PCR for diagnosis of invasive aspergillosis in a neutropenie rat model and effect of caspofungin acetate. Clin. Diagn. Lab. Immunol. 12:1322-1327.
39. Wilson, D., B. Yen-Lieberman, U. Reischl, I. Warshawsky, and G. W. Procop. 2004. Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens. J. Clin. Microbiol. 42:5913-5916.
40. Yera, H., L. Delhaes, D. Filisetti, T. Ancelle, P. Thulliez, and P. Bastien. 2007. Comparison of five DNA extraction methods for Toxoplasma gondii from amniotic fluid, p. 35. Abstr. Soc. Fr. Parasitol. Meet. (In French.)