PCR-induced sequence artifacts and bias: Insights from comparison of two 16s rRNA clone libraries constructed from the same sample

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 8966-8969

  Acinas, Silvia G ^a   Sarma Rupavtarm, Ramahi ^a   Klepac Ceraj, Vanja ^a   Polz, Martin F ^a,b  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; BACTERIA; CLONING; DNA; GENES; SAMPLING;

AMPLIFICATION PROTOCOLS; ARTIFACTS; BACTERIOPLANKTON SAMPLE; HETERODUPLEX MOLECULES;

RNA;

RIBOSOME RNA; RNA 16S; TAQ POLYMERASE;

BACTERIOPLANKTON; ERROR ANALYSIS; GENETIC ANALYSIS; GENETIC VARIATION; POLYMERASE CHAIN REACTION;

ARTICLE; ARTIFACT; BACTERIOPLANKTON; CHIMERA; CLONE; COMPARATIVE STUDY; GENE AMPLIFICATION; GENE CLUSTER; GENETIC VARIABILITY; POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE HOMOLOGY;

ANIMALS; ARTIFACTS; EVOLUTION, MOLECULAR; GENE LIBRARY; PLANKTON; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; TAQ POLYMERASE;

EID: 29144445330     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.8966-8969.2005     Document Type: Article

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