Analysis of artifacts suggests DGGE should not be used for quantitative diversity analysis

Journal of Microbiological Methods

Volumn 92, Issue 3, 2013, Pages 256-263

  Neilson, Julia W ^a   Jordan, Fiona L ^a   Maier, Raina M ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

DGGE artifact; Microbial diversity; Multitemplate PCR; Preferential amplification

Indexed keywords

AMPLICON; ANALYTIC METHOD; ARTICLE; ARTIFACT; BACTERIUM ISOLATE; COMMUNITY STRUCTURE; DENATURING GRADIENT GEL ELECTROPHORESIS; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE; SEQUENCE ANALYSIS;

ARTIFACTS; BACTERIA; BIODIVERSITY; DENATURING GRADIENT GEL ELECTROPHORESIS; REPRODUCIBILITY OF RESULTS;

EID: 84873734899     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.12.021     Document Type: Article

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