Snake venomics: From the inventory of toxins to biology

Toxicon

Volumn 75, Issue , 2013, Pages 44-62

  Calvete, Juan J ^a  

^a INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

Author keywords

Antivenomics; Venom gland transcriptomics; Venom proteomics; Venomics

Indexed keywords

ECHITAB PLUS ICP; SNAKE VENOM; SNAKE VENOM ANTISERUM; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOMICS; CHEMICAL COMPOSITION; COLLISIONALLY ACTIVATED DISSOCIATION; DNA LIBRARY; DRUG DESIGN; DRUG EFFICACY; DRUG SYNTHESIS; ENVENOMATION; EVOLUTION; GENETIC VARIABILITY; GEOGRAPHIC DISTRIBUTION; HUMAN; IMMUNOREACTIVITY; LIQUID CHROMATOGRAPHY; NEUROTOXICITY; NONHUMAN; PHENOTYPIC VARIATION; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEOMICS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE HOMOLOGY; SNAKE VENOMICS; SYSTEMS VENOMICS; TANDEM MASS SPECTROMETRY; TOXIN ANALYSIS; TOXIN STRUCTURE; TRANSCRIPTOMICS;

SERPENTES;

ANTIVENOMICS; VENOM GLAND TRANSCRIPTOMICS; VENOM PROTEOMICS; VENOMICS;

ANIMALS; ANTIVENINS; PHYLOGENY; PROTEOMICS; SNAKE BITES; SNAKE VENOMS; SNAKES; TRANSCRIPTOME;

EID: 84885330525     PISSN: 00410101     EISSN: 18793150     Source Type: Journal    
DOI: 10.1016/j.toxicon.2013.03.020     Document Type: Article

References (182)

1. Abubakar S.B., Abubakar I.S., Habib A.G., Nasidi A., Durfa N., Yusuf P.O., Larnyang S., Garnvwa J., Sokomba E., Salako L., Laing G.D., Theakston R.D., Juszczak E., Alder N., Warrell D.A. Pre-clinical and preliminary dose-finding and safety studies to identify candidate antivenoms for treatment of envenoming by saw-scaled or carpet vipers (Echis ocellatus) in northern Nigeria. Toxicon 2010, 55:719-723. Nigeria-UK EchiTab Study Group.
2. Abubakar S.B., Abubakar I.S., Habib A.G., Nasidi A., Durfa N., Yusuf P.O., Larnyang S., Garnvwa J., Sokomba E., Salako L., Theakston R.D., Juszczak E., Alder N., Warrell D.A. Randomised controlled double-blind non-inferiority trial of two antivenoms for saw-scaled or carpet viper (Echis ocellatus) envenoming in Nigeria. PLoS Negl. Trop. Dis. 2010, 4:e767. Nigeria-UK EchiTab Study Group.
3. Alape-Girón A., Sanz L., Escolano J., Flores-Díaz M., Madrigal M., Sasa M., Calvete J.J. Snake venomics of the lancehead pitviper Bothrops asper. Geographic, individual and ontogenetic variations. J. Proteome Res. 2008, 7:3556-3571.
4. Angulo Y., Escolano J., Lomonte B., Gutiérrez J.M., Sanz L., Calvete J.J. Snake venomics of Central American pitvipers. Clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi. J. Proteome Res. 2008, 7:708-719.
5. Antúnez J., Fernández J., Lomonte B., Angulo Y., Sanz L., Pérez A., Calvete J.J., Gutiérrrez J.M. Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: relationship to the neutralization of toxic and enzymatic activities. J. Venom Res. 2010, 1:8-17.
6. Bazaa A., Marrakchi N., El Ayeb M., Sanz L., Calvete J.J. Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina. Proteomics 2005, 5:4223-4235.
7. Barlow A., Pook C.E., Harrison R.A., Wüster W. Coevolution of diet and prey-specific venom activity supports the role of selection in snake venom evolution. Proc. R. Soc. Biol. Sci. 2009, 276:2443-2449.
8. Bandeira N., Clauser K.R., Pevzner P.A. Shotgun protein sequencing. Assembly of peptide tandem mass spectra from mixtures of modified proteins. Mol. Cell. Proteomics 2007, 6:1123-1134.
9. Bernardes C., Menaldo D.L., Camacho E., Rosa J.C., Escalante T., Rucavado A., Lomonte B., Gutiérrez J.M., Sampaio S.V. Proteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinase. J. Proteomics 2013, 80:250-267.
10. Birrell G.W., Earl S., Masci P.P., de Jersey J., Wallis T.P., Gorman J.J., Lavin M.F. Molecular diversity in venom from the Australian Brown snake, Pseudonaja textilis. Mol. Cell. Proteomics 2006, 5:379-389.
11. Birrell G.W., Earl S.T., Wallis T.P., Masci P.P., de Jersey J., Gorman J.J., Lavin M.F. The diversity of bioactive proteins in Australian snake venoms. Mol. Cell. Proteomics 2007, 6:973-986.
12. Boldrini-França J., Rodrigues R.S., Fonseca F.P., Menaldo D.L., Ferreira F.B., Henrique-Silva F., Soares A.M., Hamaguchi A., Rodrigues V.M., Otaviano A.R., Homsi-Brandeburgo M.I. Crotalus durissus collilineatus venom gland transcriptome: analysis of gene expression profile. Biochimie 2009, 91:586-595.
13. Boldrini-França J., Corrêa-Netto C., Silva M.M.S., Rodrigues R.S., De La Torre P., Pérez A., Soares A.M., Zingali R.B., Nogueira R.A., Rodrigues V.M., Calvete J.J. Snake venomics and antivenomics of Crotalus durissus subspecies from Brazil: assessment of geographic variation and its implication on snakebite management. J. Proteomics 2010, 73:1758-1776.
14. Bolnick D.I., Svanback R., Araujo M.S., Persson L. Comparative support for the niche variation hypothesis that more generalized populations also are more heterogeneous. Proc. Natl. Acad. Sci. U. S. A. 2007, 104:10075-10079.
15. Branch W.R. The venomous snakes of southern Africa. Part 2. Elapidae and Hydrophiidae. Snake 1979, 11:199-225.
16. Brenner S. Hunters and gatherers. The Scientist 2002, 16:14.
17. Calvete J.J. Venomics: digging into the evolution of venomous systems and learning to twist nature to fight pathology. J. Proteomics 2009, 72:121-126.
18. Calvete J.J. Snake venomics, antivenomics, and venom phenotyping: ménage à trois of proteomic tools aimed at understanding the biodiversity of venoms. Toxins and Hemostasis: from Bench to Bedside 2010, 45-72. Springer, Dordrecht (The Netherlands). R.M. Kini, F.S. Markland, M.A. McLane, T. Morita (Eds.).
19. Calvete J.J. Proteomic tools against the neglected pathology of snake bite envenoming. Expert Rev. Proteomics 2011, 8:739-758.
20. 2 molecules. Acta Chim. Slov. 2011, 58:629-637.
21. Calvete J.J. Antivenomics and venom phenotyping: a marriage of convenience to address the performance and range of clinical use of antivenoms. Toxicon 2012, 56:1284-1291.
22. Calvete J.J. Omic technologies to fight the neglect. J. Proteomics 2012, 74:1483-1484.
23. Calvete J.J., Juárez P., Sanz L. Snake venomics. Strategy and applications. J. Mass Spectrom. 2007, 42:1405-1414.
24. Calvete J.J., Marcinkiewicz C., Sanz L. Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2. J. Proteome Res. 2007, 6:326-336.
25. Calvete J.J., Escolano J., Sanz L. Snake venomics of Bitis species reveals large intragenus venom toxin composition variation: application to taxonomy of congeneric taxa. J. Proteome Res. 2007, 6:2732-2745.
26. Calvete J.J., Fasoli E., Sanz L., Boschetti E., Righetti P.G. Exploring the venom proteome of the western diamondback rattlesnake, Crotalus atrox, via snake venomics and combinatorial peptide ligand library approaches. J. Proteome Res. 2009, 8:3055-3067.
27. Calvete J.J., Sanz L., Angulo Y., Lomonte B., Gutiérrez J.M. Venoms, venomics, antivenomics. FEBS Lett. 2009, 583:1736-1743.
28. Calvete J.J., Borges A., Segura A., Flores-Diaz M., Alape-Girón A., Gutiérrez J.M., Díez N., DE Sousa L., Kiriakos D., Sánchez E., Faks J.G., Escolano J., Sanz L. Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: contributing to its taxonomy and snakebite management. J. Proteomics 2009, 72:227-240.
29. Calvete J.J., Sanz L., Cid P., De La Torre P., Flores-Díaz M., Dos Santos M.C., Borges A., Bremo A., Angulo Y., Lomonte B., Alape-Girón A., Gutiérrez J.M. Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America. J. Proteome Res. 2010, 9:528-544.
30. Calvete J.J., Cid P., Sanz L., Segura A., Villalta M., Herrera M., León G., Harrison R., Durfa N., Nasidi A., Theakston R.D., Warrell D.A., Gutiérrez J.M. Antivenomic assessment of the immunological reactivity of EchiTAb-Plus-ICP, an antivenom for the treatment of snakebite envenoming in sub-Saharan Africa. Am. J. Trop. Med. Hyg. 2010, 82:1194-1201.
31. Calvete J.J., Sanz L., Pérez A., Borges A., Vargas A.M., Lomonte B., Angulo Y., Gutiérrez J.M., Chalkidis H.M., Mourão R.H., Furtado M.F., Moura da Silva A.M. Snake population venomics and antivenomics of Bothrops atrox: paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management. J. Proteomics 2011, 74:510-527.
32. Calvete J.J., Ghezellou P., Paiva O., Matainaho T., Ghassempour A., Goudarzi H., Kraus F., Sanz L., Williams D.J. Snake venomics of two poorly known Hydrophiinae: comparative proteomics of the venoms of terrestrial Toxicocalamus longissimus and marine Hydrophis cyanocinctus. J. Proteomics 2012, 75:4091-4101.
33. Calvete J.J., Pérez A., Lomonte B., Sánchez E.E., Sanz L. Snake venomics of Crotalus tigris: the minimalist toxin arsenal of the deadliest neartic rattlesnake venom. Evolutionary clues for generating a pan-specific antivenom against crotalid type II venoms. J. Proteome Res. 2012, 11:1382-1390.
34. Cardoso K.C., Da Silva M.J., Costa G.G., Torres T.T., Del Bem L.E., Vidal R.O., Menossi M., Hyslop S. A transcriptomic analysis of gene expression in the venom gland of the snake Bothrops alternatus (urutu). BMC Genomics 2010, 11:605.
35. Casewell N.R., Harrison R.A., Wüster W., Wagstaff S.C. Comparative venom gland transcriptome surveys of the saw-scaled vipers (Viperidae: Echis) reveal substantial intra-family gene diversity and novel venom transcripts. BMC Genomics 2009, 10:564.
36. Casewell N.R., Wagstaff S.C., Harrison R.A., Renjifo C., Wüster W. Domain loss facilitates accelerated evolution and neofunctionalization of duplicate snake venom metalloproteinase toxin genes. Mol. Biol. Evol. 2011, 28:2637-2649.
37. Casewell N.R., Wüster W., Vonk F.J., Harrison R.A., Fry B.G. Complex cocktails: the evolutionary novelty of venoms. Trends Ecol. Evol. 2012, pii:S0169-5347(12) 00293-5.
38. Casewell N.R., Huttley G.A., Wüster W. Dynamic evolution of venom proteins in squamate reptiles. Nat. Commun. 2012, 3:1066.
39. Chatrath S.T., Chapeaurouge A., Lin Q., Lim T.K., Dunstan N., Mirtschin P., Kumar P.P., Kini R.M. Identification of novel proteins from the venom of a cryptic snake Drysdalia coronoides by a combined transcriptomics and proteomics approach. J. Proteome Res. 2011, 10:739-750.
40. Chen T., Bjourson A.J., Orr D.F., Kwok H., Rao P., Ivanyi C., Shaw C. Unmasking venom gland transcriptomes in reptile venoms. Anal. Biochem. 2002, 311:152-156.
41. Ching A.T., Rocha M.M., Paes Leme A.F., Pimenta D.C., Furtado M.F., Serrano S.M., Ho P.L., Junqueira-de-Azevedo I.L. Some aspects of the venom proteome of the Colubridae snake Philodryas olfersii revealed from a Duvernoy's (venom) gland transcriptome. FEBS Lett. 2006, 580:4417-4422.
42. Ching A.T., Paes Leme A.F., Zelanis A., Rocha M.M., Furtado M.F., Silva D.A., Trugilho M.R., da Rocha S.L., Perales J., Ho P.L., Serrano S.M., Junqueira-de-Azevedo I.L. Venomics profiling of Thamnodynastes strigatus unveils matrix metalloproteinases and other novel proteins recruited to the toxin arsenal of rear-fanged snakes. J. Proteome Res. 2012, 11:1152-1162.
43. Chippaux J.-P., Williams V., White J. Snake venom variability: methods of study, results and interpretation. Toxicon 1991, 29:1279-1303.
44. Cidade D.A., Simão T.A., Dávila A.M., Wagner G., Junqueira-de-Azevedo I.L., Ho P.L., Bon C., Zingali R.B., Albano R.M. Bothrops jararaca venom gland transcriptome: analysis of the gene expression pattern. Toxicon 2006, 48:437-461.
45. Ciscotto P.H., Rates B., Silva D.A., Richardson M., Silva L.P., Andrade H., Donato M.F., Cotta G.A., Maria W.S., Rodrigues R.J., Sanchez E., De Lima M.E., Pimenta A.M. Venomic analysis and evaluation of antivenom cross-reactivity of South American Micrurus species. J. Proteomics 2011, 74:1810-1825.
46. Corrêa-Netto C., Teixeira-Araujo R., Aguiar A.S., Melgarejo A.R., De Simone S.G., Soares M.R., Foguel D., Zingali R.B. Immunome and venome of Bothrops jararacussu: a proteomic approach to study the molecular immunology of snake toxins. Toxicon 2010, 55:1222-1235.
47. Corrêa-Netto C., Junqueira-de-Azevedo I.L., Silva D.A., Ho P.L., Leitão-de-Araújo M., Alves M.L., Sanz L., Foguel D., Zingali R.B., Calvete J.J. Snake venomics and venom gland transcriptomic analysis of Brazilian coral snakes, Micrurus altirostris and M. corallinus. J. Proteomics 2011, 74:1795-1809.
48. Cottrell J.S. Protein identification using MS/MS data. J. Proteomics 2011, 74:1842-1851.
49. Currier R.B., Calvete J.J., Sanz L., Harrison R.A., Rowley P.D., Wagstaff S.C. Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. PLoS One 2012, 7:e41888.
50. Daltry J.C., Wüster W., Thorpe R.S. Diet and snake venom evolution. Nature 1996, 379:537-540.
51. Diz A.P., Martínez-Fernández M., Rolán-Álvarez E. Proteomics in evolutionary ecology: linking the genotype with the phenotype. Mol. Ecol. 2012, 21:1060-1080.
52. Dobzhansky T. Nothing in biology makes sense except in the light of evolution. Am. Biol. Teach. 1973, 35:125-129.
53. Dokmetjian J.C., Del Canto S., Vinzón S., de Jiménez Bonino M.B. Biochemical characterization of the Micrurus pyrrhocryptus venom. Toxicon 2009, 53:375-382.
54. Doley R., Tram N.N., Reza M.A., Kini R.M. Unusual accelerated rate of deletions and insertions in toxin genes in the venom glands of the pygmy copperhead (Austrelaps labialis) from kangaroo island. BMC Evol. Biol. 2008, 8:70.
55. Durban J., Juárez P., Angulo Y., Lomonte B., Flores-Díaz M., Alape-Girón A., Sasa M., Sanz L., Gutiérrez J.M., Dopazo J., Conesa A., Calvete J.J. Profiling the venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing. BMC Genomics 2011, 12:259.
56. Durban J., Pérez A., Sanz L., Gómez A., Bonilla F., Rodríguez S., Chacón D., Sasa M., Angulo Y., Gutiérrez J.M., Calvete J.J. Integrated "omics" profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus. BMC Genomics 2013, 14:234.
57. Dyson F.J. The Sun, the Genome, the Internet. Tools of Scientific Revolutions 1999, The New York Public Library, Oxford University Press, New York.
58. Fahmi L., Makran B., Pla D., Sanz L., Oukkache N., Lkhider M., Harrison R.A., Ghalim N., Calvete J.J. Venomics and antivenomics profiles of North African Cerastes cerastes and C. vipera populations reveals a potentially important therapeutic weakness. J. Proteomics 2012, 75:2442-2453.
59. Fasoli E., Sanz L., Wagstaff S., Harrison R.A., Righetti P.G., Calvete J.J. Exploring the venom proteome of the African puff adder, Bitis arietans, using a combinatorial peptide ligand library approach at different pHs. J. Proteomics 2010, 73:932-942.
60. Faure G., Xu H., Saul F.A. Crystal structure of crotoxin reveals key residues involved in the stability and toxicity of this potent heterodimeric β-neurotoxin. J. Mol. Biol. 2011, 412:176-191.
61. Fernandes D.S., Franco F.L., Fernandes R. Systematic revision of the genus Lachesis Daudain, 1803 (Serpentes, Viperidae). Herpetologica 2004, 60:245-260.
62. Fernández J., Lomonte B., Sanz L., Angulo Y., Gutiérrez J.M., Calvete J.J. Snake venomics of Bothriechis nigroviridis reveals extreme variability among palm pitviper venoms: different evolutionary solutions for the same trophic purpose. J. Proteome Res. 2010, 9:4234-4241.
63. Fernández J., Alape-Girón A., Angulo Y., Sanz L., Gutiérrez J.M., Calvete J.J., Lomonte B. Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae). J. Proteome Res. 2011, 10:1816-1827.
64. Fontana F. Traité sur le vénin de la vipere, sur les poisons amaricains, sur le laurier-cerise et sur quelques autres poisons végetaux 1781, Gibelin, Florence.
65. Fox J.W., Shannon J.D., Stefansson B., Kamiguti A.S., Theakston R.D.G., Serrano S.M.T., Camargo A.C.M., Sherman N. Role of discovery science in toxinology: examples in venom proteomics. Perspectives in Molecular Toxinology 2002, 97-108. Wiley, UK. A. Ménez (Ed.).
66. Fox J.W., Serrano S.M.T. Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases. Toxicon 2005, 45:969-985.
67. Fox J.W., Serrano S.M.T. Exploring snake venom proteomes: multifaceted analyses for complex toxin mixtures. Proteomics 2008, 8:909-920.
68. Fox J.W., Ma L., Nelson K., Sherman N.E., Serrano S.M.T. Comparison of indirect and direct approaches using ion-trap and Fourier transform ion cyclotron resonance mass spectrometry for exploring viperid venom proteomes. Toxicon 2006, 47:700-714.
69. Francischetti I.M., My-Pham V., Harrison J., Garfield M.K., Ribeiro J.M. Bitis gabonica (Gaboon viper) snake venom gland: toward a catalog for the full-length transcripts (cDNA) and proteins. Gene 2004, 337:55-69.
70. French W.J., Hayes W.K., Bush S.P., Cardwell M.D., Bader J.O., Rael E.D. Mojave toxin in venom of Crotalus helleri (Southern Pacific Rattlesnake): molecular and geographic characterization. Toxicon 2004, 44:781-791.
71. Fry B.G., Wickramaratna J.C., Hodgson W.C., Alewood P.F., Kini R.M., Ho H., Wüster W. Electrospray liquid chromatography/mass spectrometry fingerprinting of Acanthophis (death adder) venoms: taxonomic and toxinological implications. Rapid Commun. Mass. Spectrom. 2002, 16:600-608.
72. Fry B.G., Wüster W., Ramjan S.F.R., Jackson T., Martelli P., Kini R.,M. Analysis of Colubroidea snake venoms by liquid chromatography with mass spectrometry: evolutionary and toxinological implications. Rapid Commun. Mass. Spectrom. 2003, 17:2047-2062.
73. Fry B.G., Wüster W., Kini R.M., Brusic V., Khan A., Venkataraman A.P., Rooney A.P. Molecular evolution and phylogeny of elapid snake venom three-finger toxins. J. Mol. Evol. 2003, 57:110-129.
74. Fry B.G., Vidal N., Norman J.A., Vonk F.J., Scheib H., Ramjan S.F., Kuruppu S., Fung K., Hedges S.B., Richardson M.K., Hodgson W.C., Ignjatovic V., Summerhayes R., Kochva E. Early evolution of the venom system in lizards and snakes. Nature 2006, 439:584-588.
75. Fry B.G., Scheib H., van der Weerd L., Young B., McNaughtan J., Ramjan S.F., Vidal N., Poelmann R.E., Norman J.A. Evolution of an arsenal: structural and functional diversification of the venom system in the advanced snakes (Caenophidia). Mol. Cell. Proteomics 2008, 7:215-246.
76. Fry B.G., Roelants K., Champagne D.E., Scheib H., Tyndall J.D., King G.F., Nevalainen T.J., Norman J.A., Lewis R.J., Norton R.S., Renjifo C., de la Vega R.C. The toxicogenomic multiverse: convergent recruitment of proteins into animal venoms. Annu. Rev. Genomics Hum. Genet. 2009, 10:483-511.
77. Fry B.G., Scheib H., Junqueira de Azevedo I.L.M., Silva D.A., Casewell N.R. Novel transcripts in the maxillary venom glands of advanced snakes. Toxicon 2012, 59:696-708.
78. Georgieva D., Arni R.K., Betzel C. Proteome analysis of snake venom toxins: pharmacological insights. Expert Rev. Proteomics 2008, 5:787-797.
79. Georgieva D., Risch M., Kardas A., Buck F., von Bergen M., Betzel C. Comparative analysis of the venom proteomes of Vipera ammodytes ammodytes and Vipera ammodytes meridionalis. J. Proteome Res. 2008, 7:866-886.
80. Georgieva D., Ohler M., Seifert J., von Bergen M., Arni R.K., Genov N., Betzel C. Snake venomic of Crotalus durissus terrificus-correlation with pharmacological activities. J. Proteome Res. 2010, 9:2302-2316.
81. Georgieva D., Seifert J., Öhler M., von Bergen M., Spencer P., Arni R.K., Genov N., Betzel C. Pseudechis australis venomics: adaptation for a defense against microbial pathogens and recruitment of body transferrin. J. Proteome Res. 2011, 10:2440-2464.
82. 2 venom genes in closely related Sistrurus rattlesnakes with divergent diets. J. Mol. Evol. 2008, 66:151-166.
83. Gibbs H.L., Mackessy S.P. Functional basis of a molecular adaptation: prey-specific toxic effects of venom from Sistrurus rattlesnakes. Toxicon 2009, 53:672-679.
84. Gibbs H.L., Sanz L., Calvete J.J. Snake population venomics: proteomics-based analyses of individual variation reveals gene regulation effects on venom protein expression in Sistrurus rattlesnakes. J. Mol. Evol. 2009, 68:113-125.
85. Gibbs H.L., Chiucchi J.E. Deconstructing a complex molecular phenotype: population-level variation in individual venom proteins in Eastern Massasauga rattlesnakes (Sistrurus c. catenatus). J. Mol. Evol. 2011, 72:383-397.
86. Gibbs H.L., Sanz L., Chiucchi J.E., Farrell T.M., Calvete J.J. Proteomic analysis of ontogenetic and diet-related changes in venom composition of juvenile and adult Dusky Pigmy rattlesnakes (Sistrurus miliarius barbouri). J. Proteomics 2011, 74:2169-2179.
87. Glenn J.L., Straight R.C., Wolt T.B. Regional variation in the presence of canebrake toxin in Crotalus horridus venom. Comp. Biochem. Physiol. 1994, 107 C:337-346.
88. Guércio R.A., Shevchenko A., Shevchenko A., López-Lozano J.L., Paba J., Sousa M.V., Ricart C.A. Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox. Proteome Sci. 2006, 4:11.
89. Gutberlet R.L., Harvey M.B. The evolution of New World venomous snakes. The Venomous Reptiles of the Western Hemisphere 2004, 634-682. Comstock Publishing Associates, Ithaca. J.A. Campbell, W.W. Lamar (Eds.).
90. Gutiérrez J.M., Dos Santos M.C., Furtado M.F., Rojas G. Biochemical and pharmacological similarities between the venoms of newborn Crotalus durissus durissus and adult Crotalus durissus terrificus rattlesnakes. Toxicon 2001, 29:1273-1277.
91. Gutiérrez J.M., Rojas E., Quesada L., León G., Núñez J., Laing G.D., Sasa M., Renjifo J.M., Nasidi A., Warrell D.A., Theakston R.D., Rojas G. Pan-African polyspecific antivenom produced by caprylic acid purification of horse IgG: an alternative to the antivenom crisis in Africa. Trans. R. Soc. Trop. Med. Hyg. 2005, 99:468-475.
92. Gutierrez J., Sanz L., Escolano J., Fernández J., Lomonte B., Angulo Y., Rucavado A., Warrell D.A., Calvete J.J. Snake venomics of the Lesser Antillean pit vipers Bothrops caribbaeus and Bothrops lanceolatus. Correlation with toxicological activities and immunoreactivity of a heterologous antivenom. J. Proteome Res. 2008, 7:4396-4408.
93. Gutiérrez J.M. Snakebite envenomation in Central America. Handbook of Venoms and Toxins of Reptiles 2009, 491-507. CRC Press, Boca Raton. S.P. Mackessy (Ed.).
94. Gutiérrez J.M., Lomonte B., León G., Alape-Girón A., Flores-Díaz M., Sanz L., Angulo Y., Calvete J.J. Snake venomics and antivenomics: proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming. J. Proteomics 2009, 72:165-182.
95. Gutiérrez J.M., Sanz L., Flores-Díaz M., Figueroa L., Madrigal M., Herrera M., Villalta M., León G., Estrada R., Borges A., Alape-Girón A., Calvete J.J. Impact of regional variation in Bothrops asper snake venom on the design of antivenoms: integrating antivenomics and neutralization approaches. J. Proteome Res. 2010, 9:564-577.
96. Gutiérrez J.M. Improving antivenom availability and accessibility: science, technology, and beyond. Toxicon 2012, 60:676-687.
97. Gutiérrez J.M., Solano G., Pla D., Herrera M., Segura A., Villalta M., Vargas M., Sanz L., Lomonte B., Calvete J.J., León G. Assessing the preclinical efficacy of antivenoms: from the lethality neutralization assay to antivenomics. Toxicon 2013, 69:168-179. 10.1016/j.toxicon.2012.11.016.
98. Gutiérrez J.M., Tsai W.C., Pla D., Solano G., Lomonte B., Sanz L., Angulo Y., Calvete J.J. Preclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: insights from combined antivenomics and neutralization assays. Toxicon 2013, 64:60-69. 10.1016/j.toxicon.2012.12.024.
99. Habermann E., Breithaupt H. Mini-review. The crotoxin complex - an example of biochemical and pharmacological protein complementation. Toxicon 1978, 16:19-30.
100. Hawgood B.J. Abbé Felice Fontana (1730-1805): founder of modern toxinology. Toxicon 1995, 33:591-601.
101. Herrera M., Fernández J., Vargas M., Villalta M., Segura A., León G., Angulo Y., Paiva O., Matainaho T., Jensen S.D., Winkel K.D., Calvete J.J., Williams D.J., Gutiérrez J.M. Comparative proteomic analysis of the venom of the snake taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia: role of neurotoxic and procoagulant effects in venom toxicity. J. Proteomics 2012, 75:2128-2140.
102. Hughes B. African snake faunas. Bonn Zool. Beitr 1983, 34:311-356.
103. James J. The Snake Charmer. A Life and Death in the Pursuit of Knowledge 2008, Hyperion Press.
104. Jia Y., Cantu B.A., Sánchez E.E., Pérez J.C. Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma. Toxicon 2008, 51:1457-1466.
105. Jiang Y., Li Y., Lee W., Xu X., Zhang Y., Zhao R., Zhang Y., Wang W. Venom gland transcriptomes of two elapid snakes (Bungarus multicinctus and Naja atra) and evolution of toxin genes. BMC Genomics 2011, 12:1.
106. Juárez P., Sanz L., Calvete J.J. Snake venomics: characterization of protein families in Sistrurus barbouri venom by cysteine mapping, N-terminal sequencing, and tandem mass spectrometry analysis. Proteomics 2004, 4:327-338.
107. Juárez P., Wagstaff S.C., Oliver J., Sanz L., Harrison R.A., Calvete J.J. Molecular cloning of disintegrin-like transcript BA-5A from a Bitis arietans venom gland cDNA library: a putative intermediate in the evolution of the long-chain disintegrin bitistatin. J. Mol. Evol. 2006, 63:142-152.
108. Juárez P., Comas I., González-Candelas F., Calvete J.J. Evolution of snake venom disintegrins by positive Darwinian selection. Mol. Biol. Evol. 2008, 25:2391-2407.
109. Junqueira-de-Azevedo I.,L., Ho P.L. A survey of gene expression and diversity in the venom glands of the pitviper snake Bothrops insularis through the generation of expressed sequence tags (ESTs). Gene 2002, 299:279-291.
110. Junqueira-de-Azevedo I.L., Ching A.T., Carvalho E., Faria F., Nishiyama M.Y., Ho P.L., Diniz M.R. Lachesis muta (Viperidae) cDNAs reveal diverging pit viper molecules and scaffolds typical of cobra (Elapidae) venoms: implications for snake toxin repertoire evolution. Genetics 2006, 173:877-889.
111. Kamiguti A.S., Theakston R.D., Sherman N., Fox J.W. Mass spectrophotometric evidence for P-III/P-IV metalloproteinases in the venom of the Boomslang (Dispholidus typus). Toxicon 2000, 38:1613-1620.
112. Karlsson E., Eaker D.L., Porath J. Purification of a neurotoxin from the venom of Naja nigricollis. Biochim. Biophys. Acta 1966, 127:505-520.
113. 2. Biochimie 2004, 86:211-219.
114. 2 enzymes. Toxicon 2003, 42:827-840.
115. Kohlhoff M., Borges M.H., Yarleque A., Cabezas C., Richardson M., Sanchez E.F. Exploring the proteomes of the venoms of the Peruvian pit vipers Bothrops atrox, B. barnetti and B. pictus. J. Proteomics 2012, 75:2181-2195.
116. Kuhn T.S. The Structure of Scientific Revolutions 1970, The University of Chicago Press, Chicago.
117. Kulkeaw K., Chaicumpa W., Sakolvaree Y., Tongtawe P., Tapchaisri P. Proteome and immunome of the venom of the Thai cobra, Naja kaouthia. Toxicon 2007, 49:1026-1041.
118. Leão L.I., Ho P.L., Junqueira-de-Azevedo I.L. Transcriptomic basis for an antiserum against Micrurus corallinus (coral snake) venom. BMC Genomics 2009, 10:112.
119. Li M., Fry B.G., Kini R.M. Eggs-only diet: its implications for the toxin profile changes and ecology of the marbled sea snake (Aipysurus eydouxii). J. Mol. Evol. 2005, 60:81-89.
120. Li S., Wang J., Zhang X., Ren Y., Wang N., Zhao K., Chen X., Zhao C., Li X., Shao J., Yin J., West M.B., Xu N., Liu S. Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys. Biochem. J. 2004, 384:119-127.
121. Liu S., Yang F., Zhang Q., Sun M.Z., Gao Y., Shao S. "Anatomical" view of the protein composition and protein characteristics for Gloydius shedaoensis snake venom via proteomics approach. Anat. Rec. 2011, 294:273-282.
122. Lomonte B., Escolano J., Fernández J., Sanz L., Angulo Y., Gutiérrez J.M., Calvete J.J. Snake venomics and antivenomics of the arboreal neotropical pitvipers Bothriechis lateralis and Bothriechis schlegelii. J. Proteome Res. 2008, 7:2445-2457.
123. Lomonte B., Rey-Suárez P., Tsai W.-C., Angulo Y., Sasa M., Gutiérrez J.M., Calvete J.J. Snake venomics of the pitvipers Porthidium nasutum, Porthidium ophryomegas, and Cerrophidion godmani from Costa Rica: toxicological and taxonomical insights. J. Proteomics 2012, 75:1675-1689.
124. Lomonte B., Tsai W.-C., Bonilla F., Solórzano A., Solano G., Angulo Y., Gutiérrez J.M., Calvete J.J. Snake venomics and toxicological profiling of the arboreal pitviper Bothriechis supraciliaris from Costa Rica. Toxicon 2012, 59:592-599.
125. Mackessy S.P., Williams K., Ashton K.G. Ontogenetic variation in venom composition and diet of Crotalus oreganus concolor: a case of venom paedomorphosis?. Copeia 2003, 2003:769-782.
126. Mackessy S.P. Venom composition in rattlesnakes: trends and biological significance. The Biology of Rattlesnakes 2008, 495-510. Loma Linda University Press, Loma Linda, CA. W.K. Hayes, K.R. Beaman, M.D. Cardwell, S.P. Bush (Eds.).
127. Mackessy S.P. Evolutionary trends in venom composition in the Western Rattlesnakes (Crotalus viridis sensu lato): toxicity vs. tenderizers. Toxicon 2010, 55:1463-1474.
128. Madrigal M., Sanz L., Flores-Diaz M., Sasa M., Núñez V., Alape-Girón A., Calvete J.J. Snake venomics across genus Lachesis. Ontogenetic changes in the venom composition of L. stenophrys and comparative proteomics of the venoms of adult L. melanocephala and L. acrochorda. J. Proteomics 2012, 77:280-297.
129. Makran B., Fahmi L., Pla D., Sanz L., Oukkache N., Lkhider M., Ghalim N., Calvete J.J. Snake venomics of Macrovipera mauritanica from Morocco, and assessment of the para-specific immunoreactivity of an experimental monospecific and a commercial antivenoms. J. Proteomics 2012, 75:2431-2441.
130. Massey D.J., Calvete J.J., Sánchez E.E., Sanz L., Richards K., Curtis R., Boesen K. Venom variability and envenoming severity outcomes of the Crotalus scutulatus scutulatus (Mojave rattlesnake) from Southern Arizona. J. Proteomics 2012, 75:2576-2587.
131. Mayor A. The Poison King: the Life and Legend of Mithradates, Rome's Deadliest Enemy 2010, Princeton University Press, Princeton, New Jersey (USA).
132. Monod J. Le Hasard et la necessité (Essai sur la philosophie naturelle de la biologie moderne) 1970, Éditions du Seuil, Paris.
133. Nawarak J., Sinchaikul S., Wu C.-Y., Liau M.-Y., Phutrakui S., Chen S.-T. Proteomics of snake venoms from Elapidae and Viperidae families by multidimensional chromatographic methods. Electrophoresis 2003, 24:2838-2854.
134. Neiva M., Arraes F.B., de Souza J.V., Rádis-Baptista G., Prieto da Silva A.R., Walter M.E., Brigido M.M., Yamane T., López-Lozano J.L., Astolfi-Filho S. Transcriptome analysis of the Amazonian viper Bothrops atrox venom gland using expressed sequence tags (ESTs). Toxicon 2009, 53:427-436.
135. Núñez V., Cid P., Sanz L., De La Torre P., Angulo Y., Lomonte B., Gutiérrez J.M., Calvete J.M. Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism. J. Proteomics 2009, 73:57-78.
136. Öhler M., Georgieva D., Seifert J., von Bergen M., Arni R.K., Genov N., Betzel C. The venomics of Bothrops alternatus is a pool of acidic proteins with predominant hemorrhagic and coagulopathic activities. J. Proteome Res. 2010, 9:2422-2437.
137. Ohno M., Ménez R., Ogawa T., Danse J.M., Shimohigashi Y., Fromen C., Ducancel F., Zinn-Justin S., Le Du M.H., Boulain J.C., Tamiya T., Ménez A. Molecular evolution of snake toxins: is the functional diversity of snake toxins associated with a mechanism of accelerated evolution?. Prog. Nucleic Acid Res. Mol. Biol. 1998, 59:307-364.
138. Ohno M., Ogawa T., Oda-Ueda N., Chijiwa T., Hattori S. Accelerated and regional evolution of snake venom-gland isozyme. Perspectives in Molecular Toxinology 2002, 387-400. Wiley, New York. A. Menez (Ed.).
139. Olamendi-Portugal T., Batista C.V., Restano-Cassulini R., Pando V., Villa-Hernández O., Zavaleta-Martínez-Vargas A., Salas-Arruz M.C., Rodríguez de la Vega R.C., Becerril B., Possani L.D. Proteomic analysis of the venom from the fish eating coral snake Micrurus surinamensis: novel toxins, their function and phylogeny. Proteomics 2008, 8:1919-1932.
140. OmPraba G., Chapeaurouge A., Doley R., Devi K.R., Padmanaban P., Venkatraman C., Velmurugan D., Lin Q., Kini R.M. Identification of a novel family of snake venom proteins veficolins from Cerberus rynchops using a venom gland transcriptomics and proteomics approach. J. Proteome Res. 2010, 9:1882-1893.
141. Pahari S., Mackessy S.P., Kini R.M. The venom gland transcriptome of the Desert Massasauga rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea). BMC Mol. Biol. 2007, 8:115.
142. Peichoto M.E., Tavares F.L., Santoro M.L., Mackessy S.P. Venom proteomes of South and North American opisthoglyphous (Colubridae and Dipsadidae) snake species: a preliminary approach to understanding their biological roles. Comp. Biochem. Physiol. Part D Genomics Proteomics 2012, 7:361-369.
143. ® antivenom by antivenomics and neutralization approaches. J. Proteome Res. 2011, 10:1266-1280.
144. Pla D., Gutiérrez J.M., Calvete J.J. Second generation snake antivenomics: comparing immunoaffinity and immunodepletion protocols. Toxicon 2012, 60:688-699.
145. Place A.J., Abramson C.I. A quantitative analysis of the ancestral area of rattlesnakes. J. Herpetol 2004, 38:152-156.
146. Powell R.L., Lieb C.S. Perspective on venom evolution in Crotalus. The Biology of Rattlesnakes 2008, 551-556. Loma Linda University Press, Loma Linda, CA. W.K. Hayes, K.R. Beaman, M.D. Cardwell, S.P. Bush (Eds.).
147. Powell R.L., Lieb C.S., Rael E.D. Geographic distribution of Mojave toxin and Mojave toxin subunits among selected Crotalus species. The Biology of Rattlesnakes 2008, 537-550. Loma Linda University Press, Loma Linda, CA. W.K. Hayes, K.R. Beaman, M.D. Cardwell, S.P. Bush (Eds.).
148. Qinghua L., Xiaowei Z., Wei Y., Chenji L., Yijun H., Pengxin Q., Xingwen S., Songnian H., Guangmei Y. A catalog for transcripts in the venom gland of the Agkistrodon acutus: identification of the toxins potentially involved in coagulopathy. Biochem. Biophys. Res. Commun. 2006, 341:522-531.
149. Quinton L., Le Caer J.-P., Phan G., Ligny-Lemaire C., Bourdais-Jomaron J., Ducancel F., Chamot-Rooke J. Characterization of toxins within crude venoms by combined use of Fourier transform mass spectrometry and cloning. Anal. Chem. 2005, 77:6630-6639.
150. Redi F. Osservazioni Intorno alle Vipere 1664, All'Insegna della Stella, Florence, A digitalized version is freely accessible addressing. http://archive.org/details/osservazioniint00redigoog.
151. Rey-Suárez P., Núñez V., Gutiérrez J.M., Lomonte B. Proteomic and biological characterization of the venom of the redtail coral snake, Micrurus mipartitus (Elapidae), from Colombia and Costa Rica. J. Proteomics 2011, 75:655-667.
152. Risch M., Georgieva D., von Bergen M., Jehmlich N., Genov N., Arni R.K., Betzel C. Snake venomics of the Siamese Russell's viper (Daboia russelli siamensis)- relation to pharmacological activities. J. Proteomics 2009, 72:256-269.
153. Rodrigues R.S., Boldrini-França J., Fonseca F.P., de la Torre P., Henrique-Silva F., Sanz L., Calvete J.J., Rodrigues V.M. Combined snake venomics and venom gland transcriptomic analysis of Bothropoides pauloensis. J. Proteomics 2012, 75:2707-2720.
154. Rokyta D.R., Wray K.P., Lemmon A.R., Lemmon E.M., Caudle S.B. A high-throughput venom-gland transcriptome for the Eastern Diamondback Rattlesnake (Crotalus adamanteus) and evidence for pervasive positive selection across toxin classes. Toxicon 2011, 57:657-671.
155. Rokyta D.R., Lemmon A.R., Margres M.J., Aronow K. The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus). BMC Genomics 2012, 13:312.
156. Sanz L., Gibbs H.L., Mackessy S.P., Calvete J.J. Venom proteomes of closely related Sistrurus rattlesnakes with divergent diets. J. Proteome Res. 2006, 5:2098-2112.
157. Sanz L., Escolano J., Ferretti M., Biscoglio M.J., Rivera E., Crescenti E.J., Angulo Y., Lomonte B., Gutiérrez J.M., Calvete J.J. Snake venomics of the South and Central American Bushmasters. Comparison of the toxin composition of Lachesis muta gathered from proteomic versus transcriptomic analysis. J. Proteomics 2008, 71:46-60.
158. Sanz L., Ayvazyan N., Calvete J.J. Snake venomics of the Armenian mountain vipers Macrovipera lebetina obtusa and Vipera raddei. J. Proteomics 2008, 71:198-209.
159. Saravia P., Rojas E., Arce V., Guevara C., López J.C. Geographic and ontogenic variability in the venom of the neotropical rattlesnake Crotalus durissus: pathophysiological and therapeutic implications. Rev. Biol. Trop. 2002, 50:337-346.
160. ®) for the treatment of viper envenoming in sub-Saharan Africa. Toxicon 2010, 55:369-374.
161. Siang A.S., Doley R., Vonk F.J., Kini R.M. Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags. BMC Mol. Biol. 2010, 11:24.
162. St Pierre L., Woods R., Earl S., Masci P.P., Lavin M.F. Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species. Cell. Mol. Life Sci. 2005, 62:2679-2693.
163. St Pierre L., Birrell G.W., Earl S.T., Wallis T.P., Gorman J.J., de Jersey J., Masci P.P., Lavin M.F. Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata. J. Proteome Res. 2007, 6:3093-3107.
164. Tashima A.K., Sanz L., Camargo A.C., Serrano S.M., Calvete J.J. Snake venomics of the Brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy markers. J. Proteomics 2008, 71:473-485.
165. 2 from the venom of Taiwan habu (T. mucrosquamatus). Biochem. J. 1995, 311:895-900.
166. Vaiyapuri S., Wagstaff S.C., Harrison R.A., Gibbins J.M., Hutchinson E.G. Evolutionary analysis of novel serine proteases in the venom gland transcriptome of Bitis gabonica rhinoceros. PLoS One 2011, 6:e21532.
167. Valente R.H., Guimarães P.R., Junqueira M., Neves-Ferreira A.G., Soares M.R., Chapeaurouge A., Trugilho M.R., León I.R., Rocha S.L., Oliveira-Carvalho A.L., Wermelinger L.S., Dutra D.L., Leão L.I., Junqueira-de-Azevedo I.L., Ho P.L., Zingali R.B., Perales J., Domont G.B. Bothrops insularis venomics: a proteomic analysis supported by transcriptomic-generated sequence data. J. Proteomics 2009, 72:241-255.
168. Van Valen L. Morphological variation and width of ecological niche. Am. Nat. 1965, 99:377-390.
169. Villalta M., Pla D., Yang S.L., Sanz L., Segura A., Vargas M., Chen P.Y., Herrera M., Estrada R., Cheng Y.F., Lee C.D., Cerdas M., Chiang J.R., Angulo Y., León G., Calvete J.J., Gutiérrez J.M. Snake venomics and antivenomics of Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan: keys to understand the variable immune response in horses. J. Proteomics 2012, 75:5628-5645.
170. 1 integrin-binding motifs in venom metalloproteinases and a new group of putative toxins, renin-like aspartic proteases. Gene 2006, 377:21-32.
171. Wagstaff S.C., Sanz L., Juárez P., Harrison R.A., Calvete J.J. Combined snake venomics and venom gland transcriptomic analysis of the ocellated carpet viper, Echis ocellatus. J. Proteomics 2009, 71:609-623.
172. Warrell D.A. Geographical and intraspecies variation in the clinical manifestations of envenoming by snakes. Venomous Snakes: Ecology, Evolution and Snakebite 1997, 189-203. Clarendon Press, Oxford. R.S. Thorpe, W. Wüster, A. Malhotra (Eds.).
173. Weldon C.L., Mackessy S.P. Biological and proteomic analysis of venom from the Puerto Rican Racer (Alsophis portoricensis: Dipsadidae). Toxicon 2010, 55:558-569.
174. Wells J.M., McLuckey S.A. Collision-Induced Dissociation (CID) of peptides and proteins. Meth. Enzymol. 2005, 402:148-185.
175. Williams D.J., Gutiérrez J.M., Calvete J.J., Wuster W., Ratanabanangkoon K., Paiva O., Brown N.I., Casewell N.R., Harrison R.A., Rowley P.D., O'Shea M., Jensen S.D., Winkel K.D., Warrell D.A. Ending the drought: new strategies for improving the flow of affordable, effective antivenoms in Asia and Africa. J. Proteomics 2011, 74:1735-1767.
176. Wooldridge B.J., Pineda G., Banuelas-Ornelas J.J., Dagda R.K., Gasanov S.E., Rael E.D., Lieb C.S. Mojave rattlesnakes (Crotalus scutulatus scutulatus) lacking the acidic subunit DNA sequence lack Mojave toxin in their venom. Comp. Biochem. Physiol. B 2001, 130:169-179.
177. Wooten J.A., Gibbs H.L. Niche divergence and lineage diversification among closely related Sistrurus rattlesnakes. J. Evol. Biol. 2012, 25:317-328.
178. Wüster W., Broadley D.G. A new species of spitting cobra from northeastern Africa (Serpentes: Elapidae: Naja). J. Zool. Lond. 2003, 259:345-359.
179. Wüster W., Crookes S., Ineich I., Mane Y., Pook C.E., Trape J.F., Broadley D.J. The phylogeny of cobras inferred from mitochondrial DNA sequences: evolution of venom spitting and the phylogeography of the African spitting cobras (Serpentes: Elapidae: Naja nigricollis complex). Mol. Phylogenet. Evol. 2007, 45:437-453.
180. Zelanis A., Tashima A.K., Pinto A.F., Leme A.F., Stuginski D.R., Furtado M.F., Sherman N.E., Ho P.L., Fox J.W., Serrano S.M. Bothrops jararaca venom proteome rearrangement upon neonate to adult transition. Proteomics 2011, 11:4218-4228.
181. Zelanis A., Andrade-Silva D., Rocha M.M., Furtado M.F., Serrano S.M., Junqueira-de-Azevedo I.L., Ho P.L. A transcriptomic view of the proteome variability of newborn and adult Bothrops jararaca snake venoms. PLoS Negl. Trop. Dis. 2012, 6:e1554.
182. Zhang B., Liu Q., Yin W., Zhang X., Huang Y., Luo Y., Qiu P., Su X., Yu J., Hu S., Yan G. Transcriptome analysis of Deinagkistrodon acutus venomous gland focusing on cellular structure and functional aspects using expressed sequence tags. BMC Genomics 2006, 7:152.