Snake venomics, antivenomics, and venom phenotyping: The ménage à trois of proteomic tools aimed at understanding the biodiversity of venoms

Toxins and Hemostasis: From Bench to Bedside

Volumn , Issue , 2011, Pages 45-72

  Calvete, Juan J ^a  

^a INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84920126095     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-90-481-9295-3_4     Document Type: Chapter

References (103)

1. Alape-Girón, A., Sanz, L., Escolano, J., Flores-Díaz, M., Madrigal, M., Sasa, M., Calvete, J.J., 2008. Snake venomics of the lancehead pitviper Bothrops asper: geographic, individual, and ontogenetic variations. J. Proteome Res. 7, 3556-3571.
2. Angulo, Y., Escolano, J., Lomonte, B., Gutiérrez, J.M., Sanz, L., Calvete, J.J., 2008. Snake venomics of Central American pitvipers: clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi. J. Proteome Res. 7, 708-719.
3. Arce, V., Rojas, E., Ownby, C.L., Gutiérrez, J.M., 2003. Preclinical assessment of the ability of polyvalent (Crotalinae) and anticoral (Elapidae) antivenoms produced in Costa Rica to neutralize the venoms of North American snakes. Toxicon 41, 851-860.
4. Azofeifa-Cordero, G., Arce-Estrada, V., Flores-Diaz, M., Alape-Giron, A., 2008. Immunization with cDNA of a novel P-III type metalloproteinase from the rattlesnake Crotalus durissus durissus elicits antibodies which neutralize 69% of the hemorrhage induced by the whole venom. Toxicon 52, 302-308.
5. Barlow, A., Pook, C.E., Harrison, R.A., Wüster, W., 2009. Coevolution of diet and prey-specific venom activity supports the role of selection in snake venom evolution. Proc. Biol. Sci. 276, 2443-2449.
6. Bazaa, A., Marrakchi, N., El Ayeb, M., Sanz, L., Calvete, J.J., 2005. Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina. Proteomics 5, 4223-4235.
7. Billen, B., Bosmans, F., Tytgat, J., 2008. Animal peptides targeting voltage-activated sodium channels. Curr. Pharm. Des. 14, 2492-2502.
8. Bogarín, G., Romero, M., Rojas, G., Lutsch, C., Casadamont, M., Lang, J., Otero, R., Gutiérrez, J.M., 1999. Neutralization, by a monospecific Bothrops lanceolatus antivenom, of toxic activities induced by homologous and heterologous Bothrops snake venoms. Toxicon 37, 551-557.
9. Boldrini-França, J., Rodrigues, R.S., Fonseca, F.P., Menaldo, D.L., Ferreira, F.B., Henrique-Silva, F., Soares, A.M., Hamaguchi, A., Rodrigues, V.M., Otaviano, A.R., Homsi-Brandeburgo, M.I., 2009. Crotalus durissus collilineatus venom gland transcriptome: Analysis of gene expression profile. Biochimie 91, 586-595.
10. Bucher, B., Canonge, D., Thomas, L., Tyburn, B., Robbe-Vincent, A., Choumet, V., Bon, C., Ketterlé, J., Lang, J., 1997. Research group of snake bites in Martinique. Clinical indicators of envenoming and serum levels of venom antigens in patients bitten by Bothrops lanceolatus in Martinique. Trans. Royal Soc. Trop. Med. Hyg. 91, 186-190.
11. Calvete, J.J., 2009a. Venomics. J. Proteomics 72, 121-282.
12. Calvete, J.J., 2009b. Digging into the evolution of venomous systems and learning to twist nature to fight pathology. J. Proteomics 72, 121-126.
13. Calvete, J.J., Juárez, P., Sanz, L., 2007a. Snake venomics. Strategy and applications. J. Mass Spectrom. 42, 1405-1414.
14. Calvete, J.J., Marcinkiewicz, C., Sanz, L., 2007b. Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2. J. Proteome Res. 6, 326-336.
15. Calvete, J.J., Escolano, J., Sanz, L., 2007c. Snake venomics of Bitis species reveals large intragenus venom toxin composition variation: Application to taxonomy of congeneric taxa. J. Proteome Res. 6, 2732-2745.
16. Calvete, J.J., Fasoli, E., Sanz, L., Boschetti, E., Righetti, P.G., 2009a. Exploring the venom proteome of the western diamondback rattlesnake, Crotalus atrox, via snake venomics and combinatorial peptide ligand library approaches. J. Proteome Res. 8, 3055-3067.
17. Calvete, J.J., Borges, A., Segura, A., Flores-Díaz, M., Alape-Girón, A., Gutiérrez, J.M., Diez, N., De Sousa, L., Kiriakos, D., Sánchez, E., Faks, J.G., Escolano, J., Sanz, L., 2009b. Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: contributing to its taxonomy and snakebite management. J. Proteomics 72, 227-240.
18. Calvete, J.J., Sanz, L., Cid, P., De La Torre, P., Flores-Diaz, M., Dos Santos, M.C., Borges, A., Bremo, A., Angulo, Y., Lomonte, B., Alape-Giron, A., Gutiérrez, J.M., 2010. Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America. J. Proteome Res. 9, 528-544.
19. Campbell, J.A., Lamar, W.W., 2004. The Venomous Reptiles of the Western Hemisphere (vols. I and II). Cornell University Press.
20. Chippaux, J.P., Goyffon, M., 2008. Epidemiology of scorpionism: A global appraisal. Acta Trop. 107, 71-79.
21. Cidade, D.A.P., Simão, T.A., Dávila, A.M.R., Wagner, G., Junqueira-de-Azevedo, I.L.M., Ho, P.L., Bon, C., Zingali, R., Albano, R.M., 2006. Bothrops jararaca venom transcriptome: Analysis of the gene expression pattern. Toxicon 48, 437-461.
22. Clemetson, K.J., Morita, T., Kini, R.M., 2009. Classification and nomenclature of snake venom C-type lectins and related proteins. Toxicon 54, 83.
23. Dart, R.C., McNally, J., 2001. Efficacy, Safety, and Use of Snake Antivenoms in the United States. Ann. Emerg. Med. 37, 181-188.
24. De Lima, M.E., Pimenta, A.M.C., Martin-Euclaire, M.F., Zingali, R.B., 2009. Animal Toxins: State of the Art. Perspectives in Health and Biotechnology. Editora UFMG, Belo Horizonte.
25. Doley, R., Kini, R.M., 2009. Protein complexes in snake venom. Cell. Mol. Life Sci. 66, 2851-2871.
26. Escoubas, P., Quinton, L., Nicholson, G.M., 2008. Venomics: unravelling the complexity of animal venoms with mass spectrometry. J. Mass Spectrom. 43, 279-295.
27. Espino-Solís, G.P., Riaño-Umbarila, L., Becerril, B., Possani, L.D., 2009. Antidotes against venomous animals: state of the art and prospectives. J. Proteomics 72, 183-199.
28. Favreau, P., Menin, L., Michalet, S., Perret, F., Cheneval, O., Stöcklin, M., Bulet, P., Stöcklin, R., 2006. Mass spectrometry strategies for venom mapping and peptide sequencing from crude venoms: case applications with single arthropod specimen. Toxicon 47, 676-687.
29. Ferquel, E., de Haro, L., Jan, V., Guillemin, I., Jourdain, S., Teynié, A., d'Alayer, J., Choumet, V., 2007. Reappraisal of Vipera aspis venom neurotoxicity. PLoS One 21, e1194.
30. Fox, J.W., Serrano, S.M., 2005a. Snake toxins and hemostasis. Toxicon 45, 951-1181.
31. Fox, J.W., Serrano, S.M.T., 2005b. Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases. Toxicon 45, 969-985.
32. Fox, J.W., Serrano, S.M., 2007. Approaching the golden age of natural product pharmaceuticals from venom libraries: An overview of toxins and toxin-derivatives currently involved in therapeutic or diagnostic applications. Curr. Pharm. Des. 13, 2927-2934.
33. Fox, J.W., Serrano, S.M., 2009. Timeline of key events in snake venom metalloproteinase research. J. Proteomics 72, 200-209.
34. Fox, J.W., Ma, L., Nelson, K., Sherman, N.E., Serrano, S.M., 2006. Comparison of indirect and direct approaches using ion-trap and Fourier transform ion cyclotron resonance mass spectrometry for exploring viperid venom proteomes. Toxicon 47, 700-714.
35. Francischetti, I.M., My-Pham, V., Harrison, J., Garfield, M.K., Ribeiro, J.M.C., 2004. Bitis gabonica (Gaboon viper) snake venom gland: Towards a catalog of full-length transcripts (cDNA) and proteins. Gene 337, 55-69.
36. Fry, B.G., Wickramaratna, J.C., Jones, A., Alewood, P.F., Hodgson, W.C., 2001. Species and regional variations in the effectiveness of antivenom against the in vitro neurotoxicity of death adder (Acanthophis) venoms. Toxicol. Appl. Pharmacol. 175, 140-148.
37. Fry, B.G., Wüster, W., 2004. Assembling an arsenal: origin and evolution of the snake venom proteome inferred from phylogenetic analysis of toxin sequences. Mol. Biol. Evol. 21, 870-873.
38. Fry, B.G., 2005. From genome to "venome": molecular origin and evolution of the snake venom proteome inferred from phylogenetic analysis of toxin sequences and related body proteins. Genome Res. 15, 403-420.
39. Fry, B.G., Scheib, H., van der Weerd, L., Young, B., McNaughtan, J., Ramjan, S.F., Vidal, N., Poelmann, R.E., Norman, J.A., 2008. Evolution of an arsenal: Structural and functional diversification of the venom system in the advanced snakes (Caenophidia). Mol. Cell Proteomics 7(2), 215-246. Epub 2007 Sep 12.
40. Fry, B.G., Vidal, N., van der Weerd, L., Kochva, E., Renjifo, C., 2009a. Evolution and diversification of the Toxicofera reptile venom system. J. Proteomics 72, 127-136.
41. Fry, B.G., Roelants, K., Champagne, D.E., Scheib, H., Tyndall, J.D.A., King, G.F., Nevalainen, T.J., Norman, J.A., Lewis, R.J., Norton, R.S., Renjifo, C., Rodríguez de la Vega, R.C., 2009b. The toxicogenomic multiverse: convergent recruitment of proteins into animal venoms. Annu. Rev. Genomics Hum. Genet. 10, 483-511.
42. Garstang, W., 1922. The theory of recapitulation: A critical re-statement of the biogenetic law. Linn. Soc. Jour. Zool. XXXV, 81-101.
43. Guércio, R.A.P., Shevchenko, A., Shevchenko, A., López-Lozano, J.L., Paba, J., Sousa, M.V., Ricart, C.A.O., 2006. Ontogenetic variations in the venom proteome of the Amazonian snake Bothrops atrox. Proteome Sci. 4, 11.
44. Georgieva, D., Risch, M., Kardas, A., Buck, F., von Bergen, M., Betzel, C., 2008. Comparative analysis of the venom proteomes of Vipera ammodytes ammodytes and Vipera ammodytes meridionales. J. Proteome Res. 7, 866-886.
45. Gibbs, H.L., Sanz, L., Calvete, J.J., 2009. Snake population venomics: proteomics-based analyses of individual variation reveals significant gene regulation effects on venom protein expression in Sistrurus rattlesnakes. J. Mol. Evol. 68, 113-125.
46. Gutiérrez, J.M., dos Santos, M.C., Furtado, M.F., Rojas, G., 1991. Biochemical and pharmacological similarities between the venoms of newborn Crotalus durissus durissus and adult Crotalus durissus terrificus rattlesnakes. Toxicon. 29(10), 1273-1277.
47. Gutiérrez, J.M., Dos Santos, M.C., Furtado, M.F., Rojas, G., 2001. Biochemical and pharmacological similarities between the venoms of newborn Crotalus durissus durissus and adult Crotalus durissus terrificus rattlesnakes. Toxicon 29, 1273-1277.
48. Gutiérrez, J.M., Rojas, E., Quesada, L., León, G., Núñez, J., Laing, G.D., Sasa, M., Renjifo, J.M., Nasidi, A., Warrell, D.A., Theakston, R.D.G., Rojas, G., 2005. Pan-African polyspecific antivenom produced by caprylic acid purification of horse IgG: An alternative to the antivenom crisis in Africa. Trans. Roy. Soc. Trop. Med. Hyg. 99, 468-475.
49. Gutiérrez, J.M., Theakston, R.D.G., Warrell, D.A., 2006. Confronting the neglected problem of snake bite envenoming: The need for a global partnership. PLoS Med. 3, e150.
50. Gutiérrez, J.M., Sanz, L., Escolano, J., Fernández, J., Lomonte, B., Angulo, Y., Rucavado, A., Warrell, D.A., Calvete, J.J., 2008. Snake venomic of the Lesser Antillean pit vipers Bothrops caribbaeus and Bothrops lanceolatus: correlation with toxicological activities and immunoreactivity of a heterologous antivenom. J. Proteome Res. 7, 4396-3408.
51. Gutiérrez, J.M., 2009. Bothrops asper: from natural history to public health. Toxicon 54, 901-1028.
52. Gutiérrez, J.M., León, G., 2009. Snake antivenoms, in: De Lima, M.E., Pimenta, A.M.C., Martin- Euclaire, M.F., Zingali, R.B. (Eds.), Animal Toxins: State of the Art. Perspectives in Health and Biotechnology. Editora UFMG, Belo Horizonte, Brazil, pp. 393-421.
53. Gutiérrez, J.M., Lomonte, B., León, G., Alape-Girón, A., Flores-Díaz, M., Sanz, L., Angulo, Y., Calvete, J.J., 2009. Snake venomics and antivenomics: proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming. J. Proteomics 72, 227-240.
54. Gutiérrez, J.M., Sanz, L., Flores-Diaz, M., Figueroa, L., Madrigal, M., Herrera, M., Villalta, M., León, G., Estrada, R., Borges, A., Alape-Girón, A., Calvete, J.J., 2010. Impact of regional variation in Bothrops asper snake venom on the design of antivenoms: integrating antivenomics and neutralization approaches. J. Proteome Res. 9, 564-577.
55. Harvey, A.L., Bradley, K.N., Cochran, S.A., Rowan, E.G., Pratt, J.A., Quillfeldt, J.A., Jerusalinsky, D.A., 1998. What can toxins tell us for drug discovery? Toxicon 36, 1635-1640.
56. Jia, Y., Cantu, B.A., Sánchez, E.E., Pérez, J.C., 2008. Complementary DNA sequencing and identification of mRNAs from the venomous gland of Agkistrodon piscivorus leucostoma. Toxicon 51, 1457-1466.
57. Juárez, P., Sanz, L., Calvete, J.J., 2004. Snake venomics: characterization of protein families in Sistrurus barbouri venom by cysteine mapping, N-terminal sequencing, and tandem mass spectrometry analysis. Proteomics 4, 327-328.
58. Juárez, P., Wagstaff, S.C., Oliver, J., Sanz, L., Harrison, R.A., Calvete, J.J., 2006. Molecular cloning of disintegrin-like transcript BA-5A from a Bitis arietans venom gland cDNA library: A putative intermediate in the evolution of the long-chain disintegrin bitistatin. J. Mol. Evol. 63, 142-152.
59. Juárez, P., Comas, I., González-Candelas, F., Calvete, J.J., 2008. Evolution of snake venom disintegrins by positive Darwinian selection. Mol. Biol. Evol. 25, 2391-2407.
60. Judge, R.K., Henry, P.J., Mirtschin, P., Jelinek, G., Wilce, J.A., 2006. Toxins not neutralized by brown snake antivenom. Toxicol. Appl. Pharmacol. 213, 117-125.
61. Junqueira-de-Azevedo, I.L., Ho, P.L., 2002. A survey of gene expression and diversity in the venom glands of the pitviper snake Bothrops insularis through the generation of expressed sequence tags (ESTs). Gene 299, 279-291.
62. Junqueira-de-Azevedo, I.L.M., Ching, A.T.C., Carvalho, E., Faria, F., Nishiyama M.Y., Jr., Ho, P.L., Diniz, M.R.V., 2006. Lachesis muta (Viperidae) cDNAs reveal diverging pitviper molecules and scaffolds typical of cobra (Elapidae) venoms: implications in snake toxin repertoire evolution. Genetics 173, 877-889.
63. Junqueira de Azevedo, I.L.M., Diniz, M.R.V., Ho, P.L., 2009. Venom gland transcriptomic analysis, in: De Lima, M.E., Pimenta, A.M.C., Martin-Euclaire, M.F., Zingali, R.B. (Eds.), Animal Toxins: State of the Art. Perspectives in Health and Biotechnology. Editora UFMG, Belo Horizonte, Brazil, pp. 693-713.
64. 2. Biochimie 86, 211-219.
65. Kasturiratne, A., Wickremasinghe, A.R., de Silva, N., Gunawardena, N.K., Pathmeswaran, A., Premaratna, R., Savioli, L., Lalloo, D.G., de Silva, H.J., 2008. The global burden of snakebite: A literature analysis and modelling based on regional estimates of envenoming and deaths. PLoS Med. 5, e218.
66. Kem, W.R., Turk, T., 2009. Cnidarian toxins and venoms. Toxicon 54, 1029-1214.
67. Koh, D.C.I., Armugam, A., Jeyaseelan, K., 2007. Snake venom components and their applications in biomedicine. Cell. Mol. Life Sci. 63, 3030-3041.
68. Kulkeaw K, Chaicump W, Sakolvaree Y, Tongtawe P, Tapchaisri P., 2007. Proteome and immunome of the venom of the Thai cobra, Naja kaouthia. Toxicon 49, 1026-1041.
69. Liang, S., 2008. Proteome and peptidome profiling of spider venoms. Expert Rev. Proteomics 5, 731-746.
70. Lomonte, B., Escolano, J., Fernández, J., Sanz, L., Angulo, Y., Gutiérrez, J.M., Calvete, J.J., 2008. Snake venomics and antivenomics of the arboreal neotopical pitvipers Bothriechis lateralis and Bothriehis schlegelii. J. Proteome Res. 7, 2445-2457.
71. Mackessy, S.P., 2008. Venom composition in rattlesnakes: Trends and biological significance, in: Hayes, W.K., Beaman, K.R., Cardwell, M.D., Bush, S.P. (Eds.), The Biology of Rattlesnakes. Loma Linda University Press, Loma Linda, CA, pp. 495-510.
72. Mackessy, S.P., 2009. Handbook of Venoms and Toxins of Reptiles. CRC Press, Taylor & Francis, Boca Ratón, FL, pp. 1-507.
73. Nei, M., Gu, X., Sitnikova, T., 1997. Evolution by the birth-and-death process in multigene families of the vertebrate immune system. Proc. Natl. Acad. Sci. U.S.A. 94, 7799-7806.
74. Neiva, M., Arraes, F.B., de Souza, J.V., Rádis-Baptista, G., Prieto da Silva, A.R., Walter, M.E., Brigido, M. de M., Yamane, T., López-Lozano, J.L., Astolfi-Filho, S., 2009. Transcriptome analysis of the Amazonian viper Bothrops atrox venom gland using expressed sequence tags (ESTs). Toxicon 53, 427-436.
75. Norton, R.S., Olivera, B.M., 2006. Conotoxins down under. Toxicon 48, 789-798.
76. Núñez, V., Cid, P., Sanz, L., De La Torre, P., Angulo, Y., Lomonte, B., Gutiérrez, J.M., Calvete, J.J., 2009. Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism. J. Proteomics 73, 57-78.
77. Ohno, M., Menez, R., Ogawa, T., Danse, J.M., Shimohigashi, Y., Fromen, C., Ducancel, F., Zinn- Justin, S., Le Du, M.H., Boulain, J.C., Tamiya, T., Menez, A., 1998. Molecular evolution of snake toxins: is the functional diversity of snake toxins associated with a mechanism of accelerated evolution? Prog. Nucleic Acid Res. Mol. Biol. 59, 307-364.
78. Olivera, B.M., 2006. Conus peptides: biodiversity-based discovery and exogenomics. J. Biol. Chem. 281, 31173-31177.
79. Olivera, B.M., Bulaj, G., Garrett, J., Terlau, H., Imperial, J., 2009. Peptide toxins from the venoms of cone snails and other toxoglossan gastropods, in: De Lima, M.E., Pimenta, A.M.C., Martin- Euclaire, M.F., Zingali, R.B. (Eds.), Animal Toxins: State of the Art. Perspectives in Health and Biotechnology. Editora UFMG, Belo Horizonte, Brazil, pp. 25-48.
80. Pahari, S., Mackessy, S.P., Kini, R.M., 2007. Rattlesnake (Sistrurus catenatus edwardsii): Towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea). BMC Mol. Biol. 8, 115.
81. Qinghua, L., Xiaowei, Z., Wei, Y., Chenji, L., Yijun, H., Pengxin, Q., Xingwen, S., Songnian, H., Guangmei, Y., 2006. A catalog for transcripts in the venom gland of the Agkistrodon acutus: identification of the toxins potentially involved in coagulopathy. Biochem. Biophys. Res. Commun. 341, 522-531.
82. Richardson, W.H., Tanen, D.A., Tong, T.C., Betten, D.P., Carstairs, S.D., Williams, S.R., Cantrell, F.L., Clark, R.F., 2005. Crotalidae polyvalent immune Fab (ovine) antivenom is effective in the neutralization of South American Viperidae venoms in a murine model. Ann. Emerg. Med. 45, 595-602.
83. Risch, M., Georgieva, D., von Bergen, M., Jehmlich, N., Genov, N., Arni, R.K., Betzel, C., 2009. Snake Venomics of the Siamese Russells Viper (Daboia russelli siamensis) - relation to pharmacological activities. J. Proteomics 72, 256-269.
84. Sánchez, E.E., Ramírez, M.S., Galán, J.A., López, G., Rodríguez-Acosta, A, Pérez, J.C., 2003a. Cross reactivity of three antivenoms against North American snake venoms. Toxicon 41, 315-320.
85. Sánchez, E.E., Galán, J.A., Rodríguez-Acosta, A, Chase, P., Pérez, J.C., 2003b. Cross reactivity of three antivenoms against North American snake venoms. Toxicon 41, 357-365.
86. Sanz, L., Gibbs, H.L., Mackessy, S.P., Calvete, J.J., 2006. Venom proteomes of closely related Sistrurus rattlesnakes with divergent diets. J. Proteome Res. 5, 2098-2112.
87. Sanz, L., Escolano, J., Ferretti, M., Biscoglio, M.J., Rivera, E., Crescenti, E.J., Angulo, Y., Lomonte, B., Gutiérrez, J.M., Calvete, J.J., 2008a. Snake venomics of the South and Central American Bushmasters. Comparison of the toxin composition of Lachesis muta gathered from proteomic versus transcriptomic analysis. J. Proteomics 71, 46-60.
88. Sanz, L., Ayvazyan, N., Calvete, J.J., 2008b. Snake venomics of the Armenian mountain vipers Macrovipera lebetina obtusa and Vipera raddei. J. Proteomics 71, 198-209.
89. Saravia, P., Rojas, E., Arce, V., Guevara, C., López, J.C., Chaves, E., Velásquez, R., Rojas, G., Gutiérrez, J.M., 2002. Geographic and ontogenic variability in the venom of the neotropical rattlesnake Crotalus durissus: pathophysiological and therapeutic implications. Rev. Biol. Trop. 50, 337-346.
90. Serrano, S.M.T., Shannon, J.D., Wang, D., Camargo, A.C., Fox, J.W., 2005. A multifaceted analysis of viperid snake venoms by two-dimensional gel electrophoresis: An approach to understanding venom proteomics. Proteomics 5, 501-510.
91. Serrano, S.M.T., Fox, J.W., 2008. Exploring snake venom proteomes: multifaceted analyses for complex toxin mixtures. Proteomics 8, 909-920.
92. Stock, R.P., Massougbodji, A., Alagón, A., Chippaux, J-P., 2007. Bringing antivenoms to Sub- Saharan Africa. Nat. Biotech. 2, 173-177.
93. Tashima, A.K., Sanz, L., Camargo, A.C.M., Serrano, S.M.T., Calvete, J.J., 2008. Snake venomics of the Brazilian pitvipers Bothrops cotiara and Bothrops fonsecai. Identification of taxonomy markers. J. Proteomics 71, 473-485.
94. Theakston, R.D.G., Warrell, D.A., 2000. Crisis in snake antivenom supply for Africa. Lancet 356, 2104.
95. Thomas, L., Tyburn, B., 1996. The research group of snake bite in Martinique. Bothrops lanceolatus bites in Martinique: clinical aspects and treatment, in: Bon, C., Goyffon, M. (Eds.), Envenomings and Their Treatments. Fondation Marcel Mérieux, Lyon, pp. 255-265.
96. Valente, R. H., Guimarães, P.R., Junqueira, M., Neves-Ferreira, A.G.C., Soares, M.R., Chapeaurouge, A., Trugilho, M., León, I.R., Rocha, S., Oliveira-Carvalho, A.L., Serrão, L.W., Dutra, D., Leão, L.I., Junqueira-de-Azevedo, I., Ho, P.L., Zingali, R.B., Perales, J., Domont, G.B., 2009. Bothrops insularis venomics: A proteomic analysis supported by transcriptomicgenerated sequence data. J. Proteomics 72, 241-255.
97. Vetter, R.S., Isbister, G.K., 2008. Medical aspects of spider bites. Annu. Rev. Entomol. 53, 409- 429.
98. Vidal, N., 2002. Colubroid systematics: evidence for an early appearance of the venom apparatus followed by extensive evolutionary tinkering. J. Toxicol. Toxin Rev. 21, 21-41.
99. Wagstaff, S.C., Harrison, R.A., 2006. Venom gland EST analysis of the saw-scaled viper, Echis ocellatus, reveals novel α9β1 integrin-binding motifs in venom metalloproteinases and a new group of putative toxins, renin-like proteases. Gene 377, 21-32.
100. Wagstaff, S.C., Sanz, L., Juárez, P., Harrison, R.A., Calvete, J.J., 2009. Combined snake venomics and venom gland transcriptomic analysis of the ocellated carpet viper, Echis ocellatus. J. Proteomics 71, 609-623.
101. World Health Organization, 2007. Rabies and Envenomings. A Neglected Public Health Issue: Report of a Consultative Meeting. WHO, Geneva. Available: http://www.who.int/bloodproducts/animal-sera/Rabies.pdf.
102. Wood, D.L., Miljenovíc, T., Cai, S., Raven, R.J., Kaas, Q., Escoubas, P., Herzig, V., Wilson, D., King, G.F., 2009. ArachnoServer: A database of protein toxins from spiders. BMC Genomics 10, 375.
103. Zhang, B., Liu, Q., Yin, W., Zhang, X., Huang, Y., Luo, Y., Qiu, P., Su, X., Yu, J., Hu, S., Yan, G., 2006. Transcriptome analysis of Deinagkistrodon acutus venomous gland focusing on cellular structure and functional aspects using expressed sequence tags. BMC Genomics 7, 152.