A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

BMC Biochemistry

Volumn 14, Issue 1, 2013, Pages

  Lacombe Harvey, Marie Ève ^a   Fortin, Mélanie ^a   Ohnuma, Takayuki ^b   Fukamizo, Tamo ^b   Letzel, Thomas ^c   Brzezinski, Ryszard ^a  

^a UNIVERSITÉ DE SHERBROOKE   (Canada)

^b KINKI UNIVERSITY   (Japan)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Arginine; Chitosanase; Enzyme substrate interaction; Glycoside hydrolase family GH46; Inverting mechanism; Substrate inhibition

Indexed keywords

AMINO ACID; ARGININE; CHITOSAN; CHITOSANASE; MUTANT PROTEIN;

ARTICLE; BIOCATALYSIS; CONTROLLED STUDY; DEACETYLATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; GENE MUTATION; NONHUMAN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; STREPTOMYCES;

STREPTOMYCES SP.;

ARGININE; BIOCATALYSIS; CHITOSAN; GLYCOSIDE HYDROLASES; KINETICS; MASS SPECTROMETRY; MUTAGENESIS, SITE-DIRECTED; PROTEIN UNFOLDING; RECOMBINANT PROTEINS; STREPTOMYCES; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84883768454     PISSN: None     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-14-23     Document Type: Article

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