A validated 1H NMR method for the determination of the degree of deacetylation of chitosan

Journal of Pharmaceutical and Biomedical Analysis

Volumn 32, Issue 6, 2003, Pages 1149-1158

  Lavertu, M ^a   Xia, Z ^b   Serreqi, A N ^c   Berrada, M ^c   Rodrigues, A ^c   Wang, D ^c   Buschmann, M D ^a   Gupta, Ajay ^c,d  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c INSTITUTE FOR RESEARCH IN IMMUNOLOGY AND CANCER   (Canada)

^d NONE   (Canada)

Author keywords

Chitin; Chitosan; IR, 1H NMR, degree of deacetylation; pH sensitive

Indexed keywords

CHITOSAN; CHITIN; DRUG DERIVATIVE; PROTON;

ARTICLE; DEACETYLATION; DIAGNOSTIC ACCURACY; DRUG DETERMINATION; INFRARED RADIATION; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; TITRIMETRY; ACETYLATION; CHEMISTRY; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REPRODUCIBILITY; VALIDATION STUDY;

ACETYLATION; CHITIN; CHITOSAN; MAGNETIC RESONANCE SPECTROSCOPY; PROTONS; REPRODUCIBILITY OF RESULTS;

EID: 0042203501     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0731-7085(03)00155-9     Document Type: Article

References (19)

1. Terayama H. Method of colloid titration (a new titration between polymer ions). J. Polym. Sci. 8:1952;243-253.
2. Broussignac P. Chitosan: a natural polymer not well known by the industry. Chim. Ind. Genie Chim. 99:1968;1241-1247.
3. Domszy J.G., Roberts G.A.F. Evaluation of infrared spectroscopic techniques for analysing chitosan. Makromol. Chem. 186:1985;1671-1677.
4. Miya M., Iwamoto R., Yoshikawa S., Mima S. IR spectroscopic determination of CONH content in highly deacetylated chitosan. Int. J. Biol. Macromol. 2:1980;323-324.
5. Moore G.K., Roberts G.A.F. Determination of the degree of N-acetylation of chitosan. Int. J. Biol. Macromol. 2:1980;115-116.
6. Sabnis S., Block L.H. Improved infrared spectroscopic method for the analysis of degree of n-deacetylation of chitosan. Polym. Bull. 39:1997;67-71.
7. Sannan T., Kurita K., Ogura K., Iwakura Y. Studies on chitin: 7. IR spectroscopic determination of degree of deacetylation. Polymer. 19:1978;458-459.
8. Muraki E., Yaku F., Iyoda J., Kojima H. Measurement of degree of deacetylation in D-glucosamine oligosaccharides by UV dbsorption. Biosci. Biotech. Biochem. 57:1993;1929-1930.
9. Tan S.C., Khor E., Tan T.K., Wong S.M. The degree of deacetylation of chitosan: advocating the first derivative UV-spectrophotometry method of determination. Talanta. 45:1998;713-719.
10. Domard A. Determination of N-acetyl content in chitosan samples by c.d. measurements. Int. J. Biol. Macromol. 9:1987;333-336.
11. 1H NMR spectroscopy. Polym. Bull. 26:1991;87-94.
12. 13C CP/MAS NMR spectroscopy. Int. J. Biol. Macromol. 28:2001;359-363.
13. Raymond L., Morin F.G., Marchessault R.H. Degree of deacetylation of chitosan using conductometric titration and solid-state NMR. Carbohydr. Res. 246:1993;331-336.
14. Varum K.M., Anthonsen M.W., Grasdalen H., Smidsrod O. Determination of the degree of N-acetylation and the distribution of N-acetyl groups in partially N-deacetylated chitins (chitosans) by high-field NMR spectroscopy. Carbohydr. Res. 211:1991;17-23.
15. Shigemasa Y., Matsuura H., Sashiwa H., Saimoto H. Evaluation of different absorbance ratios from infrared spectroscopy for analyzing the degree of deacetylation in chitin. Int. J. Biol. Macromol. 18:1996;237-242.
16. Niola F., Basora N., Chornet E., Vidal P.F. A rapid method for the determination of the degree of N-acetylation of chitin-chitosan samples by acid hydrolysis and HPLC. Carbohydr. Res. 238:1993;1-9.
17. Aiba S. Studies on chitosan: 1. Determination of the degree of N-acetylation of chitosan by ultraviolet spectrophotometry and gel permeation chromatography. Int. J. Biol. Macromol. 8:1986;173-176.
18. Baxter A., Dillon M., Taylor K.D.A., Roberts G.A.F. Improved method for i.r. determination of the degree of N-acetylation of chitosan. Int. J. Biol. Macromol. 14:1992;166-169.
19. Domard A., Rinaudo M. Preparation and characterization of fully deacetylated chitosan. Int. J. Biol. Macromol. 5:1983;49-52.