Quantitative fluorometric analysis of the protective effect of chitosan on thermal unfolding of catalytically active native and genetically-engineered chitosanases

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1774, Issue 8, 2007, Pages 975-984

  Roy, Sébastien ^a,b   Fortin, Mélanie ^a   Gagnon, Julie ^a   Ghinet, Mariana Gabriela ^b   LeHoux, Jean Guy ^a,c   Dupuis, Gilles ^a,c   Brzezinski, Ryszard ^a,b  

^a UNIVERSITÉ DE SHERBROOKE   (Canada)

^b UNIVERSITÉ DE SHERBROOKE   (Canada)

^c UNIVERSITY OF SHERBROOKE   (Canada)

Author keywords

Chitosan; Chitosanase; Fluorometry; Mutagenesis; Thermostability

Indexed keywords

AMINO ACID; CARBOXYMETHYLCELLULOSE; CHITOSAN; CHITOSANASE; GLYCEROL; HEPARIN; INORGANIC SALT; POLYETHYLENEIMINE; SORBITOL;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ARTICLE; CONCENTRATION RESPONSE; DEACETYLATION; DENATURATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STABILITY; EXPERIMENTAL STUDY; FLUOROMETRY; GENETIC ENGINEERING; HEAT; MOLECULAR WEIGHT; NONHUMAN; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN FOLDING; QUANTITATIVE ANALYSIS; REACTION TIME; STREPTOMYCES COELICOLOR; STRUCTURE ACTIVITY RELATION; THERMOSTABILITY; TRANSITION TEMPERATURE;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CATALYSIS; CHITOSAN; GLYCOSIDE HYDROLASES; HEAT; HYDROGEN-ION CONCENTRATION; PROTEIN DENATURATION; PROTEIN FOLDING; SPECTROMETRY, FLUORESCENCE; STREPTOMYCES COELICOLOR; STRUCTURE-ACTIVITY RELATIONSHIP;

STREPTOMYCES COELICOLOR; STREPTOMYCES SP.;

EID: 34547530043     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2007.05.016     Document Type: Article

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