Cell-free expression of a mammalian olfactory receptor and unidirectional insertion into small unilamellar vesicles (SUVs)

Biochimie

Volumn 95, Issue 10, 2013, Pages 1909-1916

  Ritz, Sandra ^a   Hulko, Michael ^b   Zerfaß, Christian ^a   May, Sylvia ^a,c   Hospach, Ingeborg ^b   Krasteva, Nadejda ^b   Nelles, Gabriele ^b   Sinner, Eva K ^a,c  

^a MAX PLANCK INSTITUTE FOR POLYMER RESEARCH   (Germany)

^b Sony Deutschland GmbH   (Germany)

^c UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Cell free protein synthesis; Olfactory receptor OR5; Proteolytic digestion assay; Rabbit reticulocyte lysate; Unidirectional membrane insertion

Indexed keywords

LIPOSOME; MEMBRANE PROTEIN; METHIONINE; SULFUR 35;

ANIMAL CELL; ARTICLE; BACTERIUM; CELL FREE SYSTEM; CELL VACUOLE; CONTROLLED STUDY; EUKARYOTIC FLAGELLUM; GENE; GENE EXPRESSION SYSTEM; GENE INSERTION; INSECT; ISOTOPE LABELING; LIPID COMPOSITION; LIPID MEMBRANE; MAMMAL; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; OLFACTORY RECEPTOR; OR5 GENE; PROTEIN DEGRADATION; RABBIT; RETICULOCYTE; SMALL UNILAMELLAR VESICLE; WHEAT GERM;

HEXAPODA; MAMMALIA; ORYCTOLAGUS CUNICULUS;

CELL-FREE PROTEIN SYNTHESIS; OLFACTORY RECEPTOR OR5; PROTEOLYTIC DIGESTION ASSAY; RABBIT RETICULOCYTE LYSATE; UNIDIRECTIONAL MEMBRANE INSERTION;

ANIMALS; CELL-FREE SYSTEM; ESCHERICHIA COLI; GENE EXPRESSION; METHIONINE; OLFACTORY RECEPTOR NEURONS; PROTEIN BIOSYNTHESIS; PROTEIN STRUCTURE, TERTIARY; PROTEOLYSIS; RABBITS; RATS; RECEPTORS, ODORANT; RETICULOCYTES; SULFUR RADIOISOTOPES; UNILAMELLAR LIPOSOMES;

EID: 84883250147     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2013.06.021     Document Type: Article

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