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See supplementary material at E-BJIOBN-5-011003 for additional information about the zeta potential of adsorbed BSA and cBSA on glass demonstrating that the positive charge effect of cationized BSA is even stronger in water than in DPBS (Fig. 8). Fluorescence images of fluorescent labelled cBSA and the corresponding phase contrast images of adsorbed GUVs showed that the cBSA layer is intact after vesicle immobilization (Fig. 9). Long time stability of immobilized GUVs (DOPC/ DPPG) on cBSA-113 was observed for GUVs with 5-10 mol% negatively charged DPPG [Figs. 10(a) and 10(b)], whereas pure DOPC vesicles were less stable [Fig. 10(a)]. Increasing the concentration of negatively charged DPPG (0, 5, 10, 20, 30%) in GUVs also resulted in stronger surface interaction with the positively charged cBSA-113 and vesicle fusion.
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