Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL: MscL does not need the insertase YidC for insertion in vitro

Biochimica et Biophysica Acta - Biomembranes

Volumn 1808, Issue 1, 2011, Pages 41-46

  Berrier, Catherine ^a   Guilvout, Ingrid ^b,c   Bayan, Nicolas ^c,d   Park, Kyu Ho ^a   Mesneau, Agnes ^a   Chami, Mohamed ^c,d   Pugsley, Anthony P ^b,c   Ghazi, Alexandre ^a  

^a INSTITUTE FOR INTEGRATIVE BIOLOGY OF THE CELL I2BC   (France)

^b INSTITUT PASTEUR   (France)

^c CNRS   (France)

^d UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Cell free synthesis; Insertase; Ion channel; Liposome; Membrane protein

Indexed keywords

BACTERIAL PROTEIN; ION CHANNEL; LIPOSOME; MEMBRANE PROTEIN; PROTEIN MSCL; PROTEIN YIDC; UNCLASSIFIED DRUG;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; ELECTRON MICROSCOPY; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; FREEZE ETCHING; IN VITRO STUDY; LIPID BILAYER; LIPID VESICLE; NONHUMAN; OLIGOMERIZATION; PATCH CLAMP; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SUCROSE DENSITY GRADIENT CENTRIFUGATION; SUCROSE GRADIENT;

BIOCHEMISTRY; CELL-FREE SYSTEM; CHLOROPLASTS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FREEZE FRACTURING; ION CHANNELS; LIPID BILAYERS; LIPIDS; LIPOSOMES; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA; OSMOSIS; PATCH-CLAMP TECHNIQUES; PEPTIDES;

EID: 78649764518     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2010.09.018     Document Type: Article

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