Detergent binding explains anomalous SDS-PAGE migration of membrane proteins

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 6, 2009, Pages 1760-1765

  Rath, Arianna ^a,b   Glibowicka, Mira ^a   Nadeau, Vincent G ^a,b   Chen, Gong ^a,b   Deber, Charles M ^a,b  

^a HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Detergent binding; Gel shifting; Membrane proteins; SDS PAGE; Sodium dodecyl sulfate

Indexed keywords

DETERGENT; DODECYL SULFATE SODIUM; HELIX LOOP HELIX PROTEIN; MEMBRANE PROTEIN; MUTANT PROTEIN; TRANSMEMBRANE CONDUCTANCE REGULATOR;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CORRELATION ANALYSIS; GEL MOBILITY SHIFT ASSAY; HYDRODYNAMICS; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; SOLVATION; WILD TYPE;

CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MEMBRANE PROTEINS; MOLECULAR WEIGHT; MUTANT PROTEINS; PEPTIDE FRAGMENTS; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN CONFORMATION; REPRODUCIBILITY OF RESULTS;

EID: 60549094781     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0813167106     Document Type: Article

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