Recent advances in plant-virus interaction with emphasis on small interfering RNAs (siRNAs)

Molecular Biotechnology

Volumn 55, Issue 1, 2013, Pages 63-77

  Sharma, Namisha ^a   Sahu, Pranav Pankaj ^a   Puranik, Swati ^a   Prasad, Manoj ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

DNA methylation; Gene silencing; Host virus interaction; Plant virus; Small RNA

Indexed keywords

DNA METHYLATION; GENE SILENCING; HOST-VIRUS INTERACTIONS; PLANT VIRUS; SMALL RNA;

ALKYLATION; GENE EXPRESSION; METHYLATION; RNA;

VIRUSES;

ARGONAUTE PROTEIN; COMPLEMENTARY RNA; DICER; DOUBLE STRANDED RNA; PLANT DNA; RNA DIRECTED RNA POLYMERASE; SMALL INTERFERING RNA; VIRUS DNA;

ANTIVIRAL ACTIVITY; BIOLOGICAL FUNCTIONS; ENZYME INHIBITION; GENE CONTROL; GENE EXPRESSION; GENE INDUCTION; GENE SILENCING; NEPOVIRUS; NICOTIANA BENTHAMIANA; NONHUMAN; PLANT; PLANT VIRUS; POTYVIRUS; REVIEW; RNA METHYLATION; VIRUS CELL INTERACTION; VIRUS GENE; VIRUS GENOME; VIRUS INFECTION;

DNA VIRUSES;

EID: 84882454955     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9615-7     Document Type: Review

References (110)

1. Broderson, P., Sakvarelidze-Achard, L., Bruun-Rasmussen, M., Dunoyer, P., Yamamoto, Y. Y., Sieburth, L., et al. (2008). Widespread translational inhibition by plant miRNAs and siRNAs. Science, 320, 1185-1190.
2. Hannon, G. J. (2002). RNA interferences. Nature, 418, 244-251.
3. Chapman, E. J., & Carrington, J. C. (2007). Specialization and evolution of endogenous small RNA pathways. Nature Reviews Genetics, 8, 884-896.
4. Chan, S. W. L., Henderson, I. R., & Jacobsen, S. E. (2005). Gardening the genome: DNA methylation in Arabidopsis thaliana. Nature Review, 6, 351-360.
5. Carmell, M. A., & Hannon, G. J. (2004). RNase III enzymes and the initiation of gene silencing. Nature Structural & Molecular Biology, 11, 214-218.
6. Baulcombe, D. (2004). RNA silencing in plants. Nature, 431, 356-363.
7. Malone, C. D., & Hannon, G. J. (2009). Small RNAs as guardians of the genome. Cell, 136, 656-668.
8. Pantaleo, V., Szittya, G., & Burgyan, J. (2007). Molecular bases of viral RNA targeting by viral small interfering RNA-programmed RISC. Journal of Virology, 81, 3797-3806.
9. Tomari, Y., Du, T., Haley, B., Schwarz, D. S., Bennett, R., Cook, A. H., et al. (2004). RISC assembly defects in the Drosophila RNAi mutant armitage. Cell, 116, 831-841.
10. Bender, J. (2004). DNA methylation and epigenetics. Annual Review of Plant Biology, 55, 41-68.
11. Chen, X. (2009). Small RNAs and their roles in plant development. Annual review of Phytopathology, 35, 21-44.
12. Prins, M., Laimer, M., Noris, E., Schubert, J., Wassenegger, M., & Tepfer, M. (2008). Strategies for antiviral resistance in transgenic plants. Molecular Plant Pathology, 1, 73-83.
13. Lindbo, J. A., & Dougherty, W. G. (1992). Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts. Virology, 189, 725-733.
14. Shepherd, D. N., Martin, D. P., & Jennifer, A. T. (2009). Transgenic strategies for developing crop resistant to geminiviruses. Plant Science, 176, 1-11.
15. Mangwende, T. (2000). The effect of mutant replication proteins and antisense RNA to the gene on replication, Ph.D. Thesis, University of Cape Town, Cape Town, South Africa.
16. Baulcombe, D. C. (1996). Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell, 8, 1833-1844.
17. Guo, H. S., & Garcia, J. A. (1997). Delayed resistance to plum pox potyvirus mediated by a mutated RNA replicase gene: Involvement of a gene-silencing mechanism. Molecular Plant-Microbe Interaction, 10, 160-170.
18. Ratcliff, F., Harrison, B. D., & Baulcombe, D. C. (1997). A similarity between viral defense and gene silencing in plants. Science, 276, 1558-1560.
19. Hamilton, A. J., & Baulcombe, D. C. (1999). A species of small antisense RNA in posttranscriptional gene silencing in plants. Science, 286, 950-952.
20. Ruiz-Ferrer, V., & Voinnet, O. (2009). Roles of plant small RNAs in biotic stress responses. Annual Review of Plant Biology, 60, 485-510.
21. Ahlquist, P. (2006). Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses. Nature Reviews Microbiology, 4, 371-382.
22. Molnar, A., Csorba, T., Lakatos, L., Varallyay, E., Lacomme, C., & Burgyan, J. (2005). Plant virus-derived small interfering RNAs originate predominantly from highly structured single-stranded viral RNAs. Journal of Virology, 79, 7812-7818.
23. Ding, S. W., & Voinnet, O. (2007). Antiviral immunity directed by small RNAs. Cell, 130, 413-426.
24. Berstein, E., Caudy, A. A., Hammond, S. M., & Hannon, G. J. (2001). Role for a bidentate ribonuclease in initiation step of RNA interference. Nature, 409, 363-366.
25. Qu, F., Ye, X., & Morris, T. J. (2008). Arabidopsis DRB4, AGO1, AGO7 and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1. Proceedings of the National Academy of Sciences, USA, 105, 14732-14737.
26. Moissiard, G., Parizotto, E. A., Himbe, R. C., & Voinnet, O. (2007). Transitivity in Arabidopsis can be primed, requires the redundant action of the antiviral Dicer-like 4 and Dicer-like 2, and is compromised by viral-encoded suppressor proteins. RNA, 13, 1268-1278.
27. Raja, P., Wolf, J. N., & Bisaro, D. M. (2010). RNA silencing directed against geminiviruses: Post-transcriptional and epigenetic components. Biochimica et Biophysica Acta, 1799, 337-351.
28. Wang, X. B., Jovel, J., Udomporn, P., Wang, Y., Wu, Q., Li, W.-X., et al. (2011). The 21-nucleotide, but not 22-nucleotide, viral secondary small interfering RNAs direct potent antiviral defense by two cooperative Argonautes in Arabidopsis thaliana. The Plant Cell, 23, 1625-1638.
29. Khvorova, A., Reynolds, A., & Jayasena, S. D. (2003). Functional siRNAs and miRNA exhibit strand bias. Cell, 115, 209-216.
30. Orban, T. I., & Izaurralde, E. (2005). Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome. RNA, 11, 459-469.
31. Ruiz-Medrano, R., Xoconostle-Cazares, B., & Kragler, F. (2004). The plasmodesmata transport pathway for homeotic proteins, silencing signals and viruses. Current Opinion in Plant Biology, 7, 641-650.
32. Mlotshwa, S., Voineet, O., Mette, M. F., Matzke, M., Vaucheret, H., Ding, S. W., et al. (2002). RNA silencing and the mobile silencing signal. Plant Cell, 14, S289-S301.
33. Vance, V., & Vaucheret, H. (2001). RNA silencing in plants-defense and counter defense. Science, 292, 2277-2280.
34. Mourrain, P., Beclin, C., Elmayan, T., Feuerbach, F., Godon, C., Morel, J. B., et al. (2000). Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell, 101, 533-542.
35. Herr, A., Jensen, M. B., Dalmay, T., & Baulcombe, D. C. (2005). RNA polymerase IV directs silencing of endogenous DNA. Science, 308, 118-120.
36. Kanno, T., Huettel, B., Mette, M. F., Aufsatz, W., Jaligot, E., et al. (2005). Atypical RNA polymerase subunits required for RNA-directed DNA methylation. Nature Genetics, 37, 761-765.
37. Dunoyer, P., Himber, C., & Voinnet, O. (2005). DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal. Nature Genetics, 37, 1356-1360.
38. Lister, R., O'Malley, R. C., Tonti-Filippini, J., Gregory, B. D., Berry, C. C., Millar, A. H., et al. (2008). Highly integrated single-base resolution maps of the epigenome in Arabidopsis. Cell, 133, 523-536.
39. Huang, L., Jones, A. M., Searle, I., Patel, K., Vogler, H., et al. (2009). An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II. Nature Structure and Molecular Biology, 16, 91-93.
40. Yadav, R. K., & Chattopadhyay, D. (2011). Enhanced viral intergenic region-specific short interfering RNA accumulation and DNA methylation correlates with resistance against a Geminivirus. Molecular Plant and Microbe Interactions, 24, 1189-1197.
41. Glick, E., Zrarchya, A., Levy, Y., Mett, A., Gidoni, D., et al. (2008). Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein. Proceedings of the National Academy of Sciences USA, 105, 157-161.
42. Goto, K., Kobori, T., Kosaka, Y., Natsuaki, T., & Masuta, C. (2007). Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Plant Cell Physiology, 7, 1050-1060.
43. Lozsa, R., Csorba, T., Lakatos, L., & Burgyan, J. (2008). Inhibition of 3′ modification of small RNAs in virus infected plants require spatial and temporal coexpression of small RNAs and viral silencing-suppressor proteins. Nucleic Acids Research, 36, 4099-4107.
44. Vargason, J. M., Szittya, G., Burgyan, J., & Tanaka Hall, T. M. (2003). Size selective recognition of siRNA by an RNA silencing suppressor. Cell, 115, 799-811.
45. Chao, J. A., Lee, J. H., Chapados, B. R., Debler, E. W., Schneemann, A., & Williamson, J. R. (2005). Dual modes of RNA-silencing suppression by flock house virus protein B2. Nature Structural & Molecular Biology, 12, 952-957.
46. Singh, G., Korde, R., Malhotra, P., Mukherjee, S., & Bhatnagar, R. K. (2010). Systematic deletion and site-directed mutagenesis of FHVB2 establish the role of C-terminal amino acid residues in RNAi suppression. Biochemical and Biophysical Research Communications, 398, 290-295.
47. Bortolamiol, D., Pazhouhandeh, M., Marrocco, K., Genschik, P., & Ziegler-Graff, V. (2007). The Polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing. Current Biology, 17, 1615-1621.
48. Lozano-Duran, R., & Bejarano, E. R. (2011). Geminivirus C2 protein might be the key player for geminiviral co-option of SCF-mediated ubiquitination. Plant Signal Behaviour, 7, 999-1001.
49. Wang, L. Y., Lin, S. S., Hung, T. H., Li, T. K., Lin, N. C., & Shen, T. L. (2012). Multiple domains of the Tobacco mosaic virus p126 protein can independently suppress local and systemic RNA silencing. Molecular Plant-Microbe Interactions, 25, 648-657.
50. Chellappan, P., Vanitharani, R., Pita, J., & Fauquet, C. M. (2004). Short interfering RNA accumulation correlates with host recovery in DNA virus-infected hosts, and gene silencing targets specific viral sequences. Journal of Virology, 78, 7465-7477.
51. Haas, G., Azevedo, J., Moissiard, G., Geldreich, A., Himber, C., et al. (2008). Nuclear import of CaMV P6 is required for infection and suppression of the RNA silencing factor DRB4. EMBO Journal, 15, 2012-2102.
52. Raja, P., Sanville, B. C., Buchmann, R. C., & Bisaro, D. M. (2008). Viral genome methylation as an epigenetic defense against geminiviruses. Journal of Virology, 82, 8997-9007.
53. Buchmann, R. C., Asad, S., Wolf, J. N., Mohannath, G., & Bisaro, D. M. (2009). Geminivirus AL2 and L2 proteins suppress transcriptional gene silencing and cause genome-wide reductions in cytosine methylation. Journal of Virology, 83, 5005-5013.
54. Ahlquist, P. (2002). RNA-dependent RNA polymerases, viruses and RNA silencing. Science, 296, 1270-1273.
55. Donaire, L., Wang, Y., Gonzalez-Ibeas, D., Mayer, K. F., Aranda, M. A., & Llave, C. (2009). Deep-sequencing of plant viral small RNAs reveals effective and widespread targeting of viral genomes. Virology, 392, 203-214.
56. Qi, X., Bao, F. S., & Xie, Z. (2009). Small RNA deep sequencing reveals role for Arabidopsis thaliana RNA-dependent RNA polymerases in viral siRNA biogenesis. PLoS ONE, 4(3), e4971.
57. Ho, T., Wang, H., Pallett, D., & Dalmay, T. (2007). Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins. FEBS Letter, 581, 3267-3272.
58. Szittya, G., Moxon, S., Pantaleo, V., Toth, G., Rusholme Pilcher, R. L., Moulton, V., et al. (2010). Structural and functional analysis of viral siRNAs. PLoS Pathogen, 6, e1000838.
59. Takeda, A., Iwasaki, S., Watanabe, T., Utsumi, M., & Watanabe, Y. (2008). The mechanism selecting the guide strand from small RNA duplexes is different among argonaute proteins. Plant Cell Physiology, 49, 493-500.
60. Wang, X. B., Wu, Q., Ito, T., Cillo, F., Li, W. X., Chen, X., et al. (2010). RNAi-mediated viral immunity requires amplification of virus-derived siRNAs in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, USA, 107, 484-489.
61. Diaz-Pendon, J. A., Li, F., Li, W. X., & Ding, S. W. (2007). Suppression of antiviral silencing by cucumber mosaic virus 2b protein in Arabidopsis is associated with drastically reduced accumulation of three classes of viral small interfering RNAs. The Plant Cell, 19, 2053-2063.
62. Garcia-Ruiz, H., Takeda, A., Chapman, E. J., Sullivan, C. M., Fahlgren, N., Brempelis, K. J., et al. (2010). Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during turnip mosaic virus infection. Plant Cell, 22, 481-496.
63. Hagen, C., Frizzi, A., Kao, J., Jia, L., Huang, M., et al. (2011). Using small RNA sequences to diagnose, sequence, and investigate the infectivity characteristics of vegetable-infecting viruses. Archives of Virology, 156, 1209-1216.
64. Ding, S. W. (2010). RNA-based antiviral immunity. Nature Reviews Immunology, 10, 632-644.
65. Schwach, F., Vaistij, F. E., Jones, L., & Baulcombe, D. C. (2005). An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiology, 138, 1842-1852.
66. Dalmay, T., Hamilton, A., Rudd, S., Angell, S., & Baulcombe, D. C. (2000). An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell, 101, 543-553.
67. Sahu, P. P., Rai, N. K., Chakraborty, S., Singh, M., Ramesh, B., Chattopadhyay, D., et al. (2010). Tomato cultivar tolerant to Tomato leaf curl New Delhi virus infection induces virus-specific short interfering RNA accumulation and defence-associated host gene expression. Molecular Plant Pathology, 11, 531-544.
68. Sahu, P. P., Rai, N. K., Puranik, S., Roy, A., Khan, M., & Prasad, M. (2012). Dynamics of defense related components in two contrasting genotypes of tomato upon infection with Tomato Leaf Curl New Delhi Virus. Molecular Biotechnology, 52, 140-150.
69. Akbergenov, R., Si-Ammour, A., Blevins, T., Amin, I., Kutter, C., Vanderschuren, H., et al. (2006). Molecular characterization of geminivirus-derived small RNAs in different plant species. Nucleic Acids Research, 34, 462-471.
70. Hagen, C., Rojas, M. R., Kon, T., & Gilbertson, R. L. (2008). Recovery from cucurbit leaf crumple virus (family Geminiviridae, genus Begomovirus) infection is an adaptive antiviral response associated with changes in viral small RNAs. Phytopathology, 98, 1029-1037.
71. Carrillo-Tripp, J., Lozoya-Gloria, E., & Rivera-Bustamante, R. F. (2007). Symptom remission and specific resistance of pepper plants after infection by Pepper golden mosaic virus. Phytopathology, 97, 51-59.
72. Lozano-Duran, R., Rosas-DIaz, T., Luna, A. P., & Bejarano, E. R. (2011). Identification of host genes involved in geminivirus infection using a reverse genetics approach. PLoS ONE, 6, e22383.
73. Sahu, P. P., Puranik, S., Khan, M., & Prasad, M. (2012). Recent advances in tomato functional genomics: Utilization of VIGS. Protoplasma, 249(4), 1017-1027.
74. Llave, C. (2010). Virus-derived small interfering RNAs at the core of plant-virus interactions. Trends in Plant Science, 15, 701-707.
75. Deleris, A., Gallego-Bartolome, J., Bao, J., Kasschau, K., Carrington, J. C., & Voinnet, O. (2006). Hierarchical action and inhibition of plant dicer-like proteins in antiviral defense. Science, 313, 68-71.
76. Donaire, L., Barajas, D., MartInez-GarcIa, B., MartInez-Priego, L., Pagain, I., & Llave, C. (2008). Structural and genetic requirements for the biogenesis of tobacco rattle virus-derived small interfering RNAs. Journal of Virology, 82, 5167-5177.
77. Boutet, S., Vazquez, F., Liu, J., Beclin, C., Fagard, M., Gratias, A., et al. (2003). Arabidopsis HEN1: A genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Current Biology, 13, 843-848.
78. Brough, C. L., Gardiner, W. E., Inamdar, N. M., Zhang, X. Y., Ehrlich, M., & Bisaro, D. M. (1992). DNA methylation inhibits propagation of tomato golden mosaic virus DNA in transfected protoplasts. Plant Molecular Biology, 18, 703-712.
79. Ermak, G., Paszkowski, U., Wohlmuth, M., Scheid, O. M., & Paszkowski, J. (1993). Cytosine methylation inhibits replication of African cassava mosaic virus by two distinct mechanisms. Nucleic Acids Research, 21, 3445-3450.
80. Blevins, T., Rajeswaran, R., Shivaprasad, P. V., Beknazariants, D., Si-Ammour, A., Park, H. S., et al. (2006). Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Research, 34, 6233-6246.
81. Rodriguez-Negrete, E. A., Carrillo-Tripp, J., & Rivera-Bustamante, R. F. (2009). RNA silencing against geminivirus: Complementary action of posttranscriptional gene silencing and transcriptional gene silencing in host recovery. Journal of Virology, 83, 1332-1340.
82. Lee, Y., Jeon, K., Lee, J. T., Kim, S., & Kim, V. N. (2002). MicroRNA maturation: Stepwise processing and subcellular localization. EMBO Journal, 21, 4663-4670.
83. Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D., Zilberman, D., et al. (2004). Genetic and functional diversification of small RNA pathways in plants. PLoS Biology, 2, E104.
84. Vazquez, F., Gasciolli, V., Crete, P., & Vaucheret, H. (2004). The nuclear dsRNA binding protein HYL1 is required for microRNA accumulation and plant development, but not posttranscriptional transgene silencing. Current Biology, 14, 346-351.
85. Allen, E., Xie, Z., Gustafson, A. M., & Carrington, J. C. (2005). microRNA-directed phasing during transacting siRNA biogenesis in plants. Cell, 121, 207-221.
86. Borsani, O., Zhu, J., Verslues, P. E., Sunkar, R., & Zhu, J. K. (2005). Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis. Cell, 123, 1279-1291.
87. Agarwal, S. K., & Jin, H. (2010). Role of small RNAs in host-microbe interactions. Annual Review Phytopathology, 48, 225-246.
88. Houwing, S., Kamminga, L. M., Berezikov, E., Cronembold, D., Girard, A., van den Elst, H., et al. (2007). A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish. Cell, 129, 69-82.
89. Mochizuki, K., & Gorovsky, M. A. (2004). Conjugation-specific small RNAs inTetrahymena have predicted properties of scan (scn) RNAs involved in genome rearrangement. Genes & Development, 18, 2068-2073.
90. Xie, Z., & Qi, X. (2008). Diverse small RNA-directed silencing pathways in plants. Biochimica et Biophysica Acta, 1779, 720-724.
91. Ambros, V., Lee, R. C., Lavanway, A., Williams, P. T., & Jewell, D. (2003). MicroRNAs and other tiny endogenous RNAs in C. elegans. Current Biology, 13, 807-818.
92. Kuwabara, T., Hsieh, J., Nakashima, K., Taira, K., & Gage, F. H. (2004). A small modulatory dsRNA specifies the fate of adult neural stem cells. Cell, 116, 779-793.
93. Ghildiyal, M., & Zamore, P. D. (2009). Small silencing RNAs: An expanding universe. Nature Reviews Genetics, 10, 94-108.
94. Bartel, D. P. (2004). MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell, 116, 281-297.
95. Baumberger, N., & Baulcombe, D. C. (2005). Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. Proceedings of the National Academy of Sciences, USA, 102, 11928-11933.
96. Yu, B., Yang, Z., Li, J., Minakhina, S., Yang, M., Padgett, R. W., et al. (2005). Methylation as a crucial step in plant microRNA biogenesis. Science, 307, 932-935.
97. Xie, Z., Allen, E., Wilken, A., & Carrington, J. C. (2005). DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana. Proceedings of the National Academy of sciences, USA, 102, 12984-12989.
98. Yamada, K., Lin, J., Dale, J., Chen, H., Shinn, P., Palm, C. J., et al. (2003). Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 302, 842-846.
99. Qi, Y., Denli, A. M., & Hannon, G. J. (2005). Biochemical specialization within Arabidopsis RNA silencing pathways. Molecular Cell, 19, 421-428.
100. Zilberman, D., Cao, X., & Jacobsen, S. E. (2003). ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation. Science, 299, 716-719.
101. Aufsatz, W., Mette, M. F., van der Winden, J., Matzke, M., & Matzke, A. J. M. (2002). HDA6, a putative histone deacetylase needed to enhance DNA methylation induced by double-stranded RNA. EMBO Journal, 21, 6832-6841.
102. Kanno, T., Mette, D. P., Kreil, D. P., Aufsatz, W., Matzke, M., & Matzke, A. J. M. (2004). Involvement of putative SNF2 chromatinremodeling protein DRD1 in RNA-directed DNA methylation. Current Biology, 14, 801-805.
103. Cao, X., & Jacobsen, S. E. (2002). Locus-specific control of asymmetric and CpNpGmethylation by the DRM and CMT3 methyltransferase genes. Proceedings of the National Academy of Sciences, USA, 99, 16491-16498.
104. Aufsatz, W., Mette, M. F., Matzke, A. J. M., & Matzke, M. (2004). The role of MET1 in RNA-directed de novo and maintenance methylation of CG dinucleotides. Plant Molecular Biology, 54, 793-804.
105. Jackson, J. P., Lindroth, A. M., Cao, X., & Jacobsen, S. E. (2002). Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase. Nature, 416, 556-560.
106. Matzke, M. A., & Birchler, J. A. (2005). RNAi-mediated pathways in the nucleus. Nature Reviews Genetics, 6, 24-35.
107. Bian, X. Y., Rasheed, M. S., Seemanpillai, M., & Rezaian, A. (2006). Analysis of silencing escape of Tomato leaf curl virus: An evaluation of the role of DNA methylation. Molecular Plant Microbe Interaction, 19, 614-624.
108. Leibman, D., Wolf, D., Saharan, V., Zelcer, A., Arazi, T., Yoel, S., et al. (2011). A high level of transgenic viral small RNA is associated with broad potyvirus resistance in cucurbits. Molecular Plant-Microbe Interactions, 24, 1220-1228.
109. Hassani-Mehraban, A., Brenkman, A. B., van den Broek, N. J., Goldbach, R., & Kormelink, R. (2009). RNAi-mediated transgenic Tospovirus resistance broken by intraspecies silencing suppressor protein complementation. Molecular Plant Microbe Interaction, 22, 1250-1257.
110. Brodersen, P., & Voinnet, O. (2006). The diversity of RNA silencing pathways in plants. Trends Genetics, 22, 268-280.