An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal

Plant Physiology

Volumn 138, Issue 4, 2005, Pages 1842-1852

  Schwach, Frank ^a   Vaistij, Fabian E ^a,b   Jones, Louise ^a,b   Baulcombe, David C ^a  

^a SAINSBURY LABORATORY   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CROPS; ENZYME KINETICS; ENZYMES; GRAFTING (CHEMICAL); PLANTS (BOTANY); RNA; VIRUSES;

NICOTIANA BENTHAMIANA; POLYMERASE; RNA SILENCING; SILENCING SIGNAL;

GENETIC ENGINEERING;

ENZYMES; FARM CROPS; GENETIC ENGINEERING; GRAFTS; NUCLEIC ACIDS; PLANTS; VIRUSES;

NICOTIANA BENTHAMIANA; POTATO VIRUS X; SOLANUM TUBEROSUM;

PLANT RNA; RNA DIRECTED RNA POLYMERASE; VEGETABLE PROTEIN;

ARTICLE; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; GENOTYPE; MERISTEM; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYSIOLOGY; PLANT LEAF; POTEXVIRUS; SEQUENCE HOMOLOGY; TOBACCO; TRANSGENIC PLANT; VIROLOGY;

BASE SEQUENCE; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GENOTYPE; MERISTEM; MOLECULAR SEQUENCE DATA; PHENOTYPE; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POTEXVIRUS; RNA REPLICASE; RNA, PLANT; SEQUENCE HOMOLOGY, AMINO ACID; TOBACCO;

EID: 26944466621     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.105.063537     Document Type: Article

References (49)

1. Astier-Manifacier S, Cornuet P (1974) RNA-dependent RNA polymerase in Chinese cabbage. Biochim Biophys Acta 232: 434-493
2. Baulcombe D (2004) RNA silencing in plants. Nature 431: 356-363
3. Baulcombe DC, Chapman SN, Santa Cruz S (1995) Jellyfish green fluorescent protein as a reporter for virus infections. Plant J 7: 1045-1053
4. Beclin C, Bautet S, Waterhouse P, Vaucheret H (2002) A branched pathway for transgene-induced RNA silencing in plants. Curr Biol 12: 684-688
5. Bernstein E, Caudy AA, Hammond SM, Hannon GJ (2001) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409: 363-366
6. Brennecke J, Stark A, Russell RB, Cohen SM (2005) Principles of micro-RNA target recognition. PLoS Biol 3: e85
7. Carmell MA, Hannon GJ (2004) RNase III enzymes and the initiation of gene silencing. Nat Struct Mol Biol 11: 214-218
8. Chan SW-L, Zilberman D, Xie Z, Johansen LK, Carrington JC, Jacobsen SE (2004) RNA silencing genes control de novo DNA methylation. Science 303: 1336
9. Covey SN, Al-Kaff NS, Langara A, Turner DS (1997) Plants combat infection by gene silencing. Nature 385: 781-782
10. Dalmay T, Hamilton AJ, Rudd S, Angell S, Baulcombe DC (2000) An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell 101: 543-553
11. Dalmay TD, Horsefield R, Braunstein TH, Baulcombe DC (2001) SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis. EMBO J 20: 2069-2078
12. English JJ, Davenport GF, Elmayan T, Vaucheret H, Baulcombe DC (1997) Requirement of sense transcription for homology-dependent virus resistance and trans-inactivation. Plant J 12:597-603
13. Fagard M, Vaucheret H (2000) Systemic silencing signal(s). Plant Mol Biol 43: 285-293
14. Foster TM, Lough TJ, Emerson SJ, Lee RH, Bowman JL, Forster RLS, Lucas WJ (2002) A surveillance system regulates selective entry of RNA into the shoot apex. Plant Cell 14: 1497-1508
15. Golden TA, Schauer SE, Lang JD, Pien S, Mushegian AR, Grossniklaus U, Meinke DW, Ray A (2002) SHORT INTEGUMENTS1/SUSPENSOR1/CARPEL FACTORY, a Dicer homolog, is a maternal effect gene required for embryo development in Arabidopsis. Plant Physiol 130: 808-822
16. Haseloff J, Siemering KR, Prasher DC, Hodge S (1997) Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc Natl Acad Sci USA 94: 2122-2127
17. Herr AJ, Jensen MB, Dalmay T, Baulcombe D (2005) RNA polymerase IV directs silencing of endogenous DNA. Science 308: 118-120
18. Himber C, Dunoyer P, Moissiard G, Ritzenthaler C, Voinnet O (2003) Transitivity-dependent and -independent cell-to-cell movement of RNA silencing. EMBO J 22: 4523-4533
19. Jones L, Hamilton AJ, Voinnet O, Thomas CL, Maule AJ, Baulcombe DC (1999) RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing. Plant Cell 11: 2291-2302
20. Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, Bartel DP, Linsley PS, Johnson JM (2005) Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 433: 769-773
21. Lindbo JA, Silva-Rosales L, Proebsting WM, Dougherty WG (1993) Induction of a highly specific antiviral state in transgenic plants: implications for regulation of gene expression and virus resistance. Plant Cell 5: 1749-1759
22. ΔΔC Method. Methods 25: 402-408
23. Lu R, Malcuit I, Moffett P, Ruiz MT, Peart J, Wu A-J, Rathjen JP, Bendahmane A, Day L, Baulcombe D (2003) High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance. EMBO J 22: 5690-5699
24. Makeyev EV, Bamford DH (2002) Cellular RNA-dependent RNA polymerase involved in posttranscriptional gene silencing has two distinct activity modes. Mol Cell 10: 1417-1427
25. Matthews REF (1991) Plant Virology, Ed 3. Academic Press, San Diego
26. Mlotshwa S, Voinnet O, Mette MF, Matzke M, Vaucheret H, Ding SW, Pruss G, Vance VB (2002) RNA silencing and the mobile silencing signal. Plant Cell (Suppl) 14: S289-S301
27. Molnár A, Csorba T, Lakatos L, Várallyay É, Lacomme C, Burgyán J (2005) Plant virus derived siRNAs predominantly originate from highly structured single-stranded viral RNAs. J Virol 79: 7812-7818
28. Motamedi MR, Verdel A, Colmenares SU, Gerber SA, Gygi SP, Moazed D (2004) Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. Cell 119: 789-802
29. Mourrain P, Beclin C, Elmayan T, Feuerbach F, Godon C, Morel J-B, Jouette D, Lacombe AM, Nikic S, Picault N, et al (2000) Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell 101: 533-542
30. Palauqui JC, Elmayan T, Pollien JM, Vaucheret H (1997) Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J 16: 4738-4745
31. Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS (2004) SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis. Genes Dev 18: 2368-2379
32. Ramakers C, Ruijter JM, Lekanne Deprez RH, Moorman AFM (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCE) data. Neurosci Lett 339: 62-66
33. Ratcliff F, Harrison BD, Baulcombe DC (1997) A similarity between viral defense and gene silencing in plants. Science 276: 1558-1560
34. Ratcliff F, Martin-Hernandez AM, Baulcombe DC (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J 25: 237-245
35. Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstruction of phylogenetic trees. Mol Biol Evol 4: 406-425
36. Sambrook J, Russel DW (2001) Molecular Cloning: A Laboratory Manual, Ed 3. Cold Spring Harbor Laboratory Press, New York
37. Schiebel W, Haas B, Marinkovic S, Klanner A, Sanger HL (1993a) RNA-directed RNA polymerase from tomato leaves. I. Purification and physical properties. J Biol Chem 268: 11851-11857
38. Schiebel W, Haas B, Marinkovic S, Klanner A, Sanger HL (1993b) RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties. J Biol Chem 268: 11858-11867
39. Schiebel W, Pelissier T, Reidel L, Thalmeir S, Schiebel R, Kempe D, Lottspeich F, Sauger HL, Wassenegger M (1998) Isolation of an RNA-directed RNA polymerase-specific cDNA done from tomato. Plant Cell 10: 2087-2102
40. Schwarz DS, Hutvagner G, Haley B, Zamore PD (2002) Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol Cell 10: 537-548
41. Vaucheret H, Vazquez F, Crete P, Bartel DP (2004) The action of ARGO-NAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev 18: 1187-1197
42. Vazquez F, Vaucheret H, Rajagopalan R, Lepers C, Gasciolli V, Mallory AC, Hilbert J-L, Bartel DP, Crete P (2004) Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs. Mol Cell 16: 69-79
43. Voinnet O, Baulcombe DC (1997) Systemic signalling in gene silencing. Nature 389:553
44. Voinnet O, Lederer C, Baulcombe DC (2000) Aviral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana. Cell 103: 157-167
45. Voinnet O, Vain P, Angell S, Baulcombe DC (1998) Systemic spread of sequence-specific transgene RNA degradation is initiated by localised introduction of ectopic promoterless DNA. Cell 95: 177-187
46. White JL, Kaper JM (1989) A simple method for detection of viral satellite RNAs in small tissue samples. J Virol Methods 23: 83-94
47. Xie Z, Fan B, Chen CH, Chen Z (2001) An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense. Proc Natl Acad Sci USA 98: 6516-6521
48. Xie Z, Johansen LK, Gustafson AM, Kasschau KD, Lellis AD, Zilberman D, Jacobsen SE, Carrington JC (2004) Genetic and functional diversification of small RNA pathways in plants. PLoS Biol 2: 642-652
49. Yang SJ, Carter SA, Cole AB, Cheng NH, Nelson RS (2004) A natural variant of a host RNA-dependent RNA polymerase is associated with increased susceptibility to viruses by Nicotiana benthamiana. Proc Natl Acad Sci USA 101: 6297-6302