A similarity between viral defense and gene silencing in plants

Science

Volumn 276, Issue 5318, 1997, Pages 1558-1560

  Ratcliff, Frank ^a   Harrison, Bryan D ^b   Baulcombe, David C ^a  

^a SAINSBURY LABORATORY   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA SEQUENCE; GENE INDUCTION; PLANT; PRIORITY JOURNAL; SILENCER GENE; TRANSGENE; VIRUS INFECTION;

BLOTTING, NORTHERN; GENE SILENCING; GENES, PLANT; GENES, VIRAL; NEPOVIRUS; PLANT DISEASES; PLANT LEAVES; RNA, VIRAL; TOBACCO; TRANSGENES;

EID: 0030992349     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5318.1558     Document Type: Article

References (31)

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25. The primary inoculations were to 4-week-old seedlings maintained in a glasshouse under added artificial illumination at a temperature between 20° and 25°C.
26. Cultures of tomato black ring nepovirus and of tomato ringspot nepovirus (Wisconsin strain) were obtained from the Scottish Crop Research Institute, Dundee, UK. They were propagated by inoculation to Nicotiana sp. The crude sap of infected plants was used as inoculum for these viruses. PVX inocula consisted of sap of plants infected with in vitro transcripts of a full-length cDNA clone (pTXS) (11).
27. 32P-labeled cDNA produced by oligo dT-primed reverse transcription of purified tomato ringspot viral RNA. RNA isolation and Northern analysis were done as described previously (11). The Northern analysis images were generated on a Fuji BAS1000 phosphorimager.
28. The construct pPVX.W22 was obtained by transfer of a cDNA fragment of W22 into the Eco RV site of PVX vector cDNA in pP2C2S (11). This cDNA fragment was obtained by PCR amplification of W22 RNA1 between nucleotide positions 4431 and 5409 of the sequence of RNA1 (9).
29. 32P-labeled cDNA from the 3′ terminal 1.5 kb of the PVX genome.
30. S. N. Covey, N. S. Al-Kaff, A. Lángara, D. S. Turner, Nature 385, 781 (1997).
31. We are grateful to the Gatsby Charitable Foundation for continuing support to the Sainsbury Laboratory.