Activation of a human chromosomal replication origin by protein tethering

Nucleic Acids Research

Volumn 41, Issue 13, 2013, Pages 6460-6474

  Chen, Xiaomi ^a   Liu, Guoqi ^a   Leffak, Michael ^a  

^a WRIGHT STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; CELL CYCLE PROTEIN; CELL CYCLE PROTEIN 45; DNA BINDING PROTEIN; MINICHROMOSOME MAINTENANCE PROTEIN; MINICHROMOSOME MAINTENANCE PROTEIN 7; MYC PROTEIN; REPLICATION LICENSING FACTOR CDT1; SINGLE STRANDED DNA BINDING PROTEIN; TRANSCRIPTION FACTOR E2F1; TRANSCRIPTION FACTOR GAL4; UNCLASSIFIED DRUG;

ACETYLATION; ARTICLE; CHROMOSOME REPLICATION; DNA DENATURATION; DNA SEQUENCE; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION;

CELL CYCLE PROTEINS; CHROMOSOMES, HUMAN; DNA-BINDING PROTEINS; E2F1 TRANSCRIPTION FACTOR; GENES, MYC; HELA CELLS; HISTONE ACETYLTRANSFERASES; HUMANS; NUCLEAR PROTEINS; ORIGIN RECOGNITION COMPLEX; REPLICATION ORIGIN;

EID: 84880518655     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkt368     Document Type: Article

References (98)

1. Jacob, F., Brenner, S. and Cuzin, F. (1963) On the regulation of DNA replication in bacteria. Cold Spring Harbor Symp. Quant. Biol., 28, 329-348.
2. Masai, H., Matsumoto, S., You, Z., Yoshizawa-Sugata, N. and Oda, M. (2010) Eukaryotic chromosome DNA replication: where, when, and how? Annu. Rev. Biochem., 79, 89-130.
3. Chuang, R.Y. and Kelly, T.J. (1999) The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks. Proc. Natl Acad. Sci. USA, 96, 2656-2661. (Pubitemid 29148773)
4. Kong, D. and DePamphilis, M.L. (2001) Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit. Mol. Cell. Biol., 21, 8095-8103. (Pubitemid 33051799)
5. Lee, J.K., Moon, K.Y., Jiang, Y. and Hurwitz, J. (2001) The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein. Proc. Natl Acad. Sci. USA, 98, 13589-13594. (Pubitemid 33115942)
6. Remus, D., Beall, E.L. and Botchan, M.R. (2004) DNA topology, not DNA sequence, is a critical determinant for Drosophila ORC-DNA binding. EMBO J., 23, 897-907. (Pubitemid 38418756)
7. Vashee, S., Cvetic, C., Lu, W., Simancek, P., Kelly, T.J. and Walter, J.C. (2003) Sequence-independent DNA binding and replication initiation by the human origin recognition complex. Genes Dev., 17, 1894-1908. (Pubitemid 36944566)
8. DePamphilis, M.L., Blow, J.J., Ghosh, S., Saha, T., Noguchi, K. and Vassilev, A. (2006) Regulating the licensing of DNA replication origins in metazoa. Curr. Opin. Cell Biol., 18, 231-239.
9. Santocanale, C., Sharma, K. and Diffley, J.F. (1999) Activation of dormant origins of DNA replication in budding yeast. Genes Dev., 13, 2360-2364. (Pubitemid 29453606)
10. Santocanale, C. and Diffley, J.F. (1996) ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J., 15, 6671-6679. (Pubitemid 26413799)
11. Woodward, A.M., Gohler, T., Luciani, M.G., Oehlmann, M., Ge, X., Gartner, A., Jackson, D.A. and Blow, J.J. (2006) Excess Mcm2-7 license dormant origins of replication that can be used under conditions of replicative stress. J. Cell Biol., 173, 673-683. (Pubitemid 46165576)
12. Karnani, N. and Dutta, A. (2011) The effect of the intra-S-phase checkpoint on origins of replication in human cells. Genes Dev., 25, 621-633.
13. Chowdhury, A., Liu, G., Kemp, M., Chen, X., Katrangi, N., Myers, S., Ghosh, M., Yao, J., Gao, Y., Bubulya, P. et al. (2010) The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation. Mol. Cell. Biol., 30, 1495-1507.
14. *(AGAAT) repeats are associated with aberrant replication at the ATX10 locus and replication origin-dependent expansion at an ectopic site in human cells. Mol. Cell. Biol., 27, 7828-7838. (Pubitemid 350086416)
15. Ilves, I., Petojevic, T., Pesavento, J.J. and Botchan, M.R. (2010) Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins. Mol. Cell, 37, 247-258.
16. Costa, A., Ilves, I., Tamberg, N., Petojevic, T., Nogales, E., Botchan, M.R. and Berger, J.M. (2011) The structural basis for MCM2-7 helicase activation by GINS and Cdc45. Nat. Struct. Mol. Biol., 18, 471-477.
17. Gambus, A., Khoudoli, G.A., Jones, R.C. and Blow, J.J. (2011) MCM2-7 form double hexamers at licensed origins in Xenopus egg extract. J. Biol. Chem., 286, 11855-11864.
18. Sansam, C.L., Cruz, N.M., Danielian, P.S., Amsterdam, A., Lau, M.L., Hopkins, N. and Lees, J.A. A vertebrate gene, ticrr, is an essential checkpoint and replication regulator. Genes Dev., 24, 183-194.
19. Kumagai, A., Shevchenko, A. and Dunphy, W.G. (2010) Treslin collaborates with TopBP1 in triggering the initiation of DNA replication. Cell, 140, 349-359.
20. Balestrini, A., Cosentino, C., Errico, A., Garner, E. and Costanzo, V. (2010) GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication. Nat. Cell Biol., 12, 484-491.
21. Kemp, M., Bae, B., Yu, J.P., Ghosh, M., Leffak, M. and Nair, S.K. (2007) Structure and function of the c-myc DNA-unwinding element-binding protein DUE-B. J. Biol. Chem., 282, 10441-10448. (Pubitemid 47093393)
22. Casper, J.M., Kemp, M.G., Ghosh, M., Randall, G.M., Vaillant, A. and Leffak, M. (2005) The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro. J. Biol. Chem., 280, 13071-13083.
23. Wang, Z., Kim, E., Leffak, M. and Xu, Y.J. (2012) Treslin, DUE-B, and GEMC1 cannot complement Sld3 mutants in fission yeast. FEMS Yeast Res., 12, 486-490.
24. Mueller, A.C., Keaton, M.A. and Dutta, A. (2011) DNA replication: mammalian Treslin-TopBP1 interaction mirrors yeast Sld3-Dpb11. Current Biol., 21, R638-R640.
25. Boos, D., Sanchez-Pulido, L., Rappas, M., Pearl, L.H., Oliver, A.W., Ponting, C.P. and Diffley, J.F. (2011) Regulation of DNA replication through Sld3-Dpb11 interaction Is conserved from yeast to humans. Current Biol., 21, 1152-1157.
26. Takeda, D.Y., Shibata, Y., Parvin, J.D. and Dutta, A. (2005) Recruitment of ORC or CDC6 to DNA is sufficient to create an artificial origin of replication in mammalian cells. Genes Dev., 19, 2827-2836. (Pubitemid 41739968)
27. Eaton, M.L., Galani, K., Kang, S., Bell, S.P. and MacAlpine, D.M. (2010) Conserved nucleosome positioning defines replication origins. Genes Dev., 24, 748-753.
28. Xu, J., Yanagisawa, Y., Tsankov, A.M., Hart, C., Aoki, K., Kommajosyula, N., Steinmann, K.E., Bochicchio, J., Russ, C., Regev, A. et al. (2012) Genome-wide identification and characterization of replication origins by deep sequencing. Genome Biol., 13, R27.
29. MacAlpine, H.K., Gordan, R., Powell, S.K., Hartemink, A.J. and MacAlpine, D.M. (2010) Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. Genome Res., 20, 201-211.
30. Chandok, G.S., Kapoor, K.K., Brick, R.M., Sidorova, J.M. and Krasilnikova, M.M. (2011) A distinct first replication cycle of DNA introduced in mammalian cells. Nucleic Acids Res., 39, 2103-2115.
31. Crevel, G. and Cotterill, S. (2012) Forced binding of the origin of replication complex to chromosomal sites in Drosophila S2 cells creates an origin of replication. J. Cell Sci., 125, 965-972.
32. Kemp, M.G., Ghosh, M., Liu, G. and Leffak, M. (2005) The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells. Nucleic Acids Res., 33, 325-336. (Pubitemid 40276954)
33. Hartl, T., Boswell, C., Orr-Weaver, T.L. and Bosco, G. (2007) Developmentally regulated histone modifications in Drosophila follicle cells: initiation of gene amplification is associated with histone H3 and H4 hyperacetylation and H1 phosphorylation. Chromosoma, 116, 197-214. (Pubitemid 46404514)
34. Wong, P.G., Glozak, M.A., Cao, T.V., Vaziri, C., Seto, E. and Alexandrow, M.G. (2010) Chromatin unfolding by Cdt1 regulates MCM loading via opposing functions of HBO1 and HDAC11-geminin. Cell Cycle, 9, 4351-4363.
35. Miotto, B. and Struhl, K. (2010) HBO1 histone acetylase activity is essential for DNA replication licensing and inhibited by Geminin. Mol. Cell, 37, 57-66.
36. Lipford, J.R. and Bell, S.P. (2001) Nucleosomes positioned by ORC facilitate the initiation of DNA replication. Mol. Cell, 7, 21-30. (Pubitemid 32162896)
37. Ghosh, M., Liu, G., Randall, G., Bevington, J. and Leffak, M. (2004) Transcription factor binding and induced transcription alter chromosomal c-myc replicator activity. Mol. Cell. Biol., 24, 10193-10207. (Pubitemid 39507859)
38. Ghosh, M., Kemp, M., Liu, G., Ritzi, M., Schepers, A. and Leffak, M. (2006) Differential binding of replication proteins across the human c-myc replicator. Mol. Cell. Biol., 26, 5270-5283. (Pubitemid 44036198)
39. Aggarwal, B.D. and Calvi, B.R. (2004) Chromatin regulates origin activity in Drosophila follicle cells. Nature, 430, 372-376. (Pubitemid 38937747)
40. Liu, G., Malott, M. and Leffak, M. (2003) Multiple functional elements comprise a mammalian chromosomal replicator. Mol. Cell. Biol., 23, 1832-1842. (Pubitemid 36246062)
41. Liu, G., Myers, S., Chen, X., Bissler, J.J., Sinden, R.R. and Leffak, M. (2012) Replication fork stalling and checkpoint activation by a PKD1 locus mirror repeat polypurine-polypyrimidine (Pu-Py) tract. J. Biol. Chem., 287, 33412-33423.
42. Royzman, I., Austin, R.J., Bosco, G., Bell, S.P. and Orr-Weaver, T.L. (1999) ORC localization in Drosophila follicle cells and the effects of mutations in dE2F and dDP. Genes Dev., 13, 827-840. (Pubitemid 29169842)
43. Taubert, S., Gorrini, C., Frank, S.R., Parisi, T., Fuchs, M., Chan, H.M., Livingston, D.M. and Amati, B. (2004) E2F-dependent histone acetylation and recruitment of the Tip60 acetyltransferase complex to chromatin in late G1. Mol. Cell. Biol., 24, 4546-4556. (Pubitemid 44109137)
44. Hiebert, S.W., Lipp, M. and Nevins, J.R. (1989) E1A-dependent trans-activation of the human MYC promoter is mediated by the E2F factor. Proc. Natl Acad. Sci. USA, 86, 3594-3598.
45. Roussel, M.F., Davis, J.N., Cleveland, J.L., Ghysdael, J. and Hiebert, S.W. (1994) Dual control of myc expression through a single DNA binding site targeted by ets family proteins and E2F-1. Oncogene, 9, 405-415. (Pubitemid 24041519)
46. Maser, R.S., Mirzoeva, O.K., Wells, J., Olivares, H., Williams, B.R., Zinkel, R.A., Farnham, P.J. and Petrini, J.H. (2001) Mre11 complex and DNA replication: linkage to E2F and sites of DNA synthesis. Mol. Cell. Biol., 21, 6006-6016. (Pubitemid 32743151)
47. Liu, G., Chen, X., Gao, Y., Lewis, T., Barthelemy, J. and Leffak, M. (2012) Altered replication in human cells promotes DMPK (CTG)(n). (CAG)(n) repeat instability. Mol. Cell. Biol., 32, 1618-1632.
48. Malott, M. and Leffak, M. (1999) Activity of the c-myc replicator at an ectopic chromosomal location. Mol. Cell. Biol., 19, 5685-5695. (Pubitemid 29339925)
49. Flemington, E.K., Speck, S.H. and Kaelin, W.G. Jr (1993) E2F-1-mediated transactivation is inhibited by complex formation with the retinoblastoma susceptibility gene product. Proc. Natl Acad. Sci. USA, 90, 6914-6918. (Pubitemid 23222358)
50. Iizuka, M., Sarmento, O.F., Sekiya, T., Scrable, H., Allis, C.D. and Smith, M.M. (2008) HBO1 links p53-dependent stress signaling to DNA replication licensing. Mol. Cell. Biol., 28, 140-153.
51. Kukimoto, I., Igaki, H. and Kanda, T. (1999) Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha. Eur. J. Biochem., 265, 936-943.
52. Nishitani, H., Taraviras, S., Lygerou, Z. and Nishimoto, T. (2001) The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase. J. Biol. Chem., 276, 44905-44911.
53. Kamath, S. and Leffak, M. (2001) Multiple sites of replication initiation in the human beta-globin gene locus. Nucleic Acids Res., 29, 809-817. (Pubitemid 32124394)
54. Liu, G., Chen, X., Bissler, J.J., Sinden, R.R. and Leffak, M. (2010) Replication-dependent instability at (CTG) x (CAG) repeat hairpins in human cells. Nat. Chem. Biol., 6, 652-659.
55. Kinoshita, Y. and Johnson, E.M. (2004) Site-specific loading of an MCM protein complex in a DNA replication initiation zone upstream of the c-MYC gene in the HeLa cell cycle. J. Biol. Chem., 279, 35879-35889. (Pubitemid 39100595)
56. Weinmann, A.S., Bartley, S.M., Zhang, T., Zhang, M.Q. and Farnham, P.J. (2001) Use of chromatin immunoprecipitation to clone novel E2F target promoters. Mol. Cell. Biol., 21, 6820-6832. (Pubitemid 32911243)
57. Bieda, M., Xu, X., Singer, MA., Green, R. and Farnham, P.J. (2006) Unbiased location analysis of E2F1-binding sites suggests a widespread role for E2F1 in the human genome. Genome Res., 16, 595-605.
58. Rabinovich, A., Jin, V.X., Rabinovich, R., Xu, X. and Farnham, PJ. (2008) E2F in vivo binding specificity: comparison of consensus versus nonconsensus binding sites. Genome Res., 18, 1763-1777.
59. Evrin, C, Clarke, P., Zech, J., Lurz, R., Sun, J., Uhle, S., Li, H., Stillman, B. and Speck, C. (2009) A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. Proc. Natl Acad. Sci. USA, 106, 20240-20245.
60. Remus, D. and Diffley, J.F. (2009) Eukaryotic DNA replication control: lock and load, then fire. Curr. Opin. Cell Biol., 21, 771-777.
61. Maiorano, D., Moreau, J. and Mechali, M. (2000) XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. Nature, 404, 622-625. (Pubitemid 30205062)
62. Rialland, M., Sola, F. and Santocanale, C. (2002) Essential role of human CDT1 in DNA replication and chromatin licensing. J. Cell. Sci., 115, 1435-1440. (Pubitemid 34428701)
63. Asano, T., Makise, M., Takehara, M. and Mizushima, T. (2007) Interaction between ORC and Cdt1p of Saccharomyces cerevisiae. FEMS Yeast Res., 7, 1256-1262. (Pubitemid 47622055)
64. Speck, C. and Stillman, B. (2007) Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication. J. Biol. Chem., 282, 11705-11714. (Pubitemid 47100659)
65. Tsuyama, T., Tada, S., Watanabe, S., Seki, M. and Enomoto, T. (2005) Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts. Nucleic Acids Res., 33, 765-775. (Pubitemid 40361006)
66. Drury, L.S. and Diffley, J.F. (2009) Factors affecting the diversity of DNA replication licensing control in eukaryotes. Curr. Biol., 19, 530-535.
67. Liontos, M., Koutsami, M., Sideridou, M., Evangelou, K., Kletsas, D., Levy, B., Kotsinas, A., Nahum, O., Zoumpourlis, V., Kouloukoussa, M. et al. (2007) Deregulated overexpression of hCdt1 and hCdc6 promotes malignant behavior. Cancer Res., 67, 10899-10909. (Pubitemid 350145919)
68. Kulartz, M. and Knippers, R. (2004) The replicative regulator protein geminin on chromatin in the HeLa cell cycle. J. Biol. Chem., 279, 41686-41694. (Pubitemid 39313614)
69. Lovejoy, CA., Lock, K., Yenamandra, A. and Cortez, D. (2006) DDB1 maintains genome integrity through regulation of Cdt1. Mol. Cell. Biol., 26, 7977-7990. (Pubitemid 44630997)
70. Arias, E.E. and Walter, J.C. (2005) Replication-dependent destruction of Cdt1 limits DNA replication to a single round per cell cycle in Xenopus egg extracts. Genes Dev., 19, 114-126. (Pubitemid 40095821)
71. Arias, E.E. and Walter, J.C. (2006) PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication. Nat. Cell Biol., 8, 84-90. (Pubitemid 43064801)
72. Arias, E.E. and Walter, J.C. (2007) Strength in numbers: preventing rereplication via multiple mechanisms in eukaryotic cells. Genes Dev., 21, 497-518. (Pubitemid 46409465)
73. Vaziri, C., Saxena, S., Jeon, Y., Lee, C., Murata, K., Machida, Y., Wagle, N., Hwang, D.S. and Dutta, A. (2003) A p53-dependent checkpoint pathway prevents rereplication. Mol. Cell, 11, 997-1008. (Pubitemid 36566321)
74. Dorn, E.S., Chastain, P.D., Hall, J.R. and Cook, J.G. (2009) Analysis of re-replication from deregulated origin licensing by DNA fiber spreading. Nucleic Acids Res., 37, 60-69.
75. Hall, J.R., Lee, H.O., Bunker, B.D., Dorn, E.S., Rogers, G.C., Duronio, R.J. and Cook, J.G. (2008) Cdt1 and Cdc6 are destabilized by rereplication-induced DNA damage. J. Biol. Chem., 283, 25356-25363.
76. Melixetian, M., Ballabeni, A., Masiero, L., Gasparini, P., Zamponi, R., Bartek, J., Lukas, J. and Helin, K. (2004) Loss of geminin induces rereplication in the presence of functional p53. J. Cell Biol., 165, 473-482. (Pubitemid 38679613)
77. Zhu, W., Chen, Y. and Dutta, A. (2004) Rereplication by depletion of geminin is seen regardless of p53 status and activates a G2/M checkpoint. Mol. Cell. Biol., 24, 7140-7150. (Pubitemid 39014440)
78. Maiorano, D., Krasinska, L., Lutzmann, M. and Mechali, M. (2005) Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts. Curr. Biol., 15, 146-153.
79. Chen, S., de Vries, M.A. and Bell, S.P. (2007) Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading. Genes Dev., 21, 2897-2907. (Pubitemid 350133438)
80. Miotto, B. and Struhl, K. (2008) HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1. Genes Dev., 22, 2633-2638.
81. Iizuka, M. and Stillman, B. (1999) Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein. J. Biol. Chem., 274, 23027-23034.
82. Burke, T.W., Cook, J.G., Asano, M. and Nevins, J.R. (2001) Replication factors MCM2 and ORC1 interact with the histone acetyltransferase HBO1. J. Biol. Chem., 276, 15397-15408.
83. Shen, Z., Chakraborty, A., Jain, A., Giri, S., Ha, T., Prasanth, K.V. and Prasanth, S.G. (2012) Dynamic association of ORCA with pre-RC components regulates DNA replication initiation. Mol. Cell. Biol., 323, 107-120.
84. Altman, A.L. and Fanning, E. (2001) The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity. Mol. Cell. Biol., 21, 1098-1110. (Pubitemid 32114959)
85. Glozak, M.A. and Seto, E. (2009) Acetylation/deacetylation modulates the stability of DNA replication licensing factor Cdt1. J. Biol. Chem., 284, 11446-11453.
86. Hasan, S. and Hottiger, M.O. (2002) Histone acetyl transferases: a role in DNA repair and DNA replication. J. Mol. Med., 80, 463-474. (Pubitemid 34983911)
87. Yang, S.C., Rhind, N. and Bechhoefer, J. (2010) Modeling genome-wide replication kinetics reveals a mechanism for regulation of replication timing. Mol. Syst. Biol., 6, 404.
88. Han, J., Zhou, H., Horazdovsky, B., Zhang, K., Xu, R.M. and Zhang, Z. (2007) Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication. Science, 315, 653-655. (Pubitemid 46214710)
89. Vashee, S., Simancek, P., Challberg, M.D. and Kelly, T.J. (2001) Assembly of the human origin recognition complex. J. Biol. Chem., 276, 26666-26673.
90. Houchens, C.R., Lu, W., Chuang, R.Y., Frattini, M.G., Fuller, A., Simancek, P. and Kelly, T.J. (2008) Multiple mechanisms contribute to Schizosaccharomyces pombe origin recognition complex-DNA interactions. J. Biol. Chem., 283, 30216-30224.
91. Cayrou, C., Coulombe, P., Puy, A., Rialle, S., Kaplan, N., Segal, E. and Mechali, M. (2012) New insights into replication origin characteristics in metazoans. Cell Cycle, 11, 658-667.
92. Altman, A.L. and Fanning, E. (2004) Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin. Mol. Cell. Biol., 24, 4138-4150. (Pubitemid 41070988)
93. Lutzmann, M. and Mechali, M. (2008) MCM9 binds Cdt1 and is required for the assembly of prereplication complexes. Mol. Cell, 31, 190-200. (Pubitemid 352001400)
94. Cook, J.G., Chasse, D.A. and Nevins, J.R. (2004) The regulated association of Cdt1 with Mcm proteins and Cdc6 in mammalian cells. J. Biol. Chem., 279, 9625-9633. (Pubitemid 38296031)
95. Nguyen, V.Q., Co, C., Irie, K. and Li, J.J. (2000) Clb/Cdc28 kinases promote nuclear export of the replication initiator proteins Mcm2-7. Curr. Biol., 10, 195-205. (Pubitemid 30146784)
96. Hopwood, B. and Dalton, S. (1996) Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication. Proc. Natl Acad. Sci. USA, 93, 12309-12314. (Pubitemid 26367139)
97. Im, J.S., Ki, S.H., Farina, A., Jung, D.S., Hurwitz, J. and Lee, J.K. (2009) Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc. Natl Acad. Sci. USA, 106, 15628-15632.
98. Fu, Y.V., Yardimci, H., Long, D.T., Ho, T.V., Guainazzi, A., Bermudez, V.P., Hurwitz, J., van Oijen, A., Scharer, O.D. and Walter, J.C. (2011) Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase. Cell, 146, 931-941.