Recruitment of ORC or CDC6 to DNA is sufficient to create an artificial origin of replication in mammalian cells

Genes and Development

Volumn 19, Issue 23, 2005, Pages 2827-2836

  Takeda, David Y ^a   Shibata, Yoshiyuki ^b   Parvin, Jeffrey D ^a   Dutta, Anindya ^a,b  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Cdc6; Episome; GAL4; Licensing; ORC; Origin; Replication

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CELL CYCLE PROTEIN 6; DNA; GEMININ; INITIATION FACTOR; PLASMID DNA; TRANSCRIPTION FACTOR GAL4;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL DIVISION; CONTROLLED STUDY; DNA BINDING; DNA REPLICATION ORIGIN; DNA REPLICATION TIMING; DNA SEQUENCE; DNA STRAND; DNA SYNTHESIS INHIBITION; EPISOME; HUMAN; HUMAN CELL; MAMMAL CELL; NONVIRAL GENE DELIVERY SYSTEM; NONVIRAL GENE THERAPY; PLASMID; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; TANDEM REPEAT;

ADENOSINE TRIPHOSPHATASES; BINDING SITES; CELL CYCLE PROTEINS; CELL LINE; DNA; HUMANS; NUCLEAR PROTEINS; ORIGIN RECOGNITION COMPLEX; PLASMIDS; RECOMBINANT FUSION PROTEINS; REPLICATION ORIGIN; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS;

EUKARYOTA; MAMMALIA;

EID: 28444493713     PISSN: 08909369     EISSN: 15495477     Source Type: Journal    
DOI: 10.1101/gad.1369805     Document Type: Article

References (44)

1. Aggarwal, B.D. and Calvi, B.R. 2004. Chromatin regulates origin activity in Drosophila follicle cells. Nature 430: 372-376.
2. Aladjem, M.I., Rodewald, L.W., Lin, C.M., Bowman, S., Cimbora, D.M., Brody, L.L., Epner, E.M., Groudine, M., and Wahl, G.M. 2002. Replication initiation patterns in the β-globin loci of totipotent and differentiated murine cells: Evidence for multiple initiation regions. Mol. Cell. Biol. 22: 442-452.
3. Altman, A.L. and Fanning, E. 2004. Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin. Mol. Cell. Biol. 24: 4138-4150.
4. Bell, S.P. and Dutta, A. 2002. DNA replication in eukaryotic cells. Annu. Rev. Biochem. 71: 333-374.
5. Bell, S.P. and Stillman, B. 1992. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357: 128-134.
6. Blow, J.J. and Dutta, A. 2005. Preventing re-replication of chromosomal DNA. Nat. Rev. Mol. Cell. Biol. 6: 476-486.
7. Chaudhuri, B., Xu, H., Todorov, I., Dutta, A., and Yates, J.L. 2001. Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus. Proc. Natl. Acad. Sci. 98: 10085-10089.
8. Cheng, L.Z., Workman, J.L., Kingston, R.E., and Kelly, T.J. 1992. Regulation of DNA replication in vitro by the transcriptional activation domain of GAL4-VP16. Proc. Natl. Acad. Sci. 89: 589-593.
9. Chuang, R.Y. and Kelly, T.J. 1999. The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks. Proc. Natl. Acad. Sci. 96: 2656-2661.
10. Cvetic, C. and Walter, J.C. 2005. Eukaryotic origins of DNA replication: Could you please be more specific? Semin. Cell Dev. Biol. 16: 343-353.
11. Danis, E., Brodolin, K., Menut, S., Maiorano, D., Girard-Reydet, C., and Mechali, M. 2004. Specification of a DNA replication origin by a transcription complex. Nat. Cell Biol. 6: 721-730.
12. Dhar, S.K. and Dutta, A. 2000. Identification and characterization of the human ORC6 homolog. J. Biol. Chem. 275: 34983-34988.
13. Dhar, S.K., Delmolino, L., and Dutta, A. 2001a. Architecture of the human origin recognition complex. J. Biol. Chem. 276: 29067-29071.
14. Dhar, S.K., Yoshida, K., Machida, Y., Khaira, P., Chaudhuri, B., Wohlschlegel, J.A., Leffak, M., Yates, J., and Dutta, A. 2001b. Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin. Cell 106: 287-296.
15. Frolova, N.S., Schek, N., Tikhmyanova, N., and Coleman, T.R. 2002. Xenopus Cdc6 performs separate functions in initiating DNA replication. Mol. Biol. Cell 13: 1298-1312.
16. Gerbi, S.A. and Bielinsky, A.K. 1997. Replication initiation point mapping. Methods 13: 271-280.
17. Giacca, M., Pelizon, C., and Falaschi, A. 1997. Mapping replication origins by quantifying relative abundance of nascent DNA strands using competitive polymerase chain reaction. Methods 13: 301-312.
18. Gilbert, D.M. 2001. Making sense of eukaryotic DNA replication origins. Science 294: 96-100.
19. -. 2004. In search of the holy replicator. Nat. Rev. Mol. Cell. Biol. 5: 848-855.
20. Harvey, K.J. and Newport, J. 2003. Metazoan origin selection: Origin recognition complex chromatin binding is regulated by CDC6 recruitment and ATP hydrolysis. J. Biol. Chem. 278: 48524-48528.
21. Hirt, B. 1967. Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 26: 365-369.
22. Huang, Y. and Kowalski, D. 2003. Web-thermodyn: Sequence analysis software for profiling DNA helical stability. Nucleic Acids Res. 31: 3819-3821.
23. Kamath, S. and Leffak, M. 2001. Multiple sites of replication initiation in the human β-globin gene locus. Nucleic Acids Res. 29: 809-817.
24. Lee, J.K., Moon, K.Y., Jiang, Y., and Hurwitz, J. 2001. The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein. Proc. Natl. Acad. Sci. 98: 13589-13594.
25. Lipford, J.R. and Bell, S.P. 2001. Nucleosomes positioned by ORC facilitate the initiation of DNA replication. Mol. Cell 7: 21-30.
26. Liu, J., Smith, C.L., DeRyckere, D., DeAngelis, K., Martin, G.S., and Berger, J.M. 2000. Structure and function of Cdc6/Cdc18: Implications for origin recognition and checkpoint control. Mol. Cell 6: 637-648.
27. Liu, G., Malott, M., and Leffak, M. 2003. Multiple functional elements comprise a mammalian chromosomal replicator. Mol. Cell. Biol. 23: 1832-1842.
28. Machida, Y.J., Teer, J.K., and Dutta, A. 2005. Acute reduction of an origin recognition complex (ORC) subunit in human cells reveals a requirement of ORC for Cdk2 activation. J. Biol. Chem. 280: 27624-27630.
29. Mendez, J. and Stillman, B. 2003. Perpetuating the double helix: Molecular machines at eukaryotic DNA replication origins. Bioessays 25: 1158-1167.
30. Ohta, S., Tatsumi, Y., Fujita, M., Tsurimoto, T., and Obuse, C. 2003. The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle. J. Biol. Chem. 278: 41535-41540.
31. Prasanth, S.G., Prasanth, K.V., and Stillman, B. 2002. Orc6 involved in DNA replication, chromosome segregation, and cytokinesis. Science 297: 1026-1031.
32. Prasanth, S.G., Prasanth, K.V., Siddiqui, K., Spector, D.L., and Stillman, B. 2004. Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance. EMBO J. 23: 2651-2663.
33. Saha, P., Chen, J., Thome, K.C., Lawlis, S.J., Hou, Z.H., Hendricks, M., Parvin, J.D., and Dutta, A. 1998. Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase. Mol. Cell. Biol. 18: 2758-2767.
34. Schaarschmidt, D., Baltin, J., Stehle, I.M., Lipps, H.J., and Knippers, R. 2004. An episomal mammalian replicon: Sequence-independent binding of the origin recognition complex. EMBO J. 23: 191-201.
35. Schepers, A. and Diffley, J.F. 2001. Mutational analysis of conserved sequence motifs in the budding yeast Cdc6 protein. J. Mol. Biol. 308: 597-608.
36. Schepers, A., Ritzi, M., Bousset, K., Kremmer, E., Yates, J.L., Harwood, J., Diffley, J.F., and Hammerschmidt, W. 2001. Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus. EMBO J. 20: 4588-4602.
37. Tada, S., Li, A., Maiorano, D., Mechali, M., and Blow, J.J. 2001. Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. Nat. Cell Biol. 3: 107-113.
38. Taylor, E.R. and Morgan, I.M. 2003. A novel technique with enhanced detection and quantitation of HPV-16 E1- and E2-mediated DNA replication. Virology 315: 103-109.
39. Vashee, S., Cvetic, C., Lu, W., Simancek, P., Kelly, T.J., and Walter, J.C. 2003. Sequence-independent DNA binding and replication initiation by the human origin recognition complex. Genes & Dev. 17: 1894-1908.
40. Vaughn, J.P., Dijkwel, P.A., and Hamlin, J.L. 1990. Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain. Cell 61: 1075-1087.
41. Waga, S. and Stillman, B. 1998. The DNA replication fork in eukaryotic cells. Annu. Rev. Biochem. 67: 721-751.
42. Witmer, X., Alvarez-Venegas, R., San-Miguel, P., Danilevskaya, O., and Avramova, Z. 2003. Putative subunits of the maize origin of replication recognition complex ZmORC1-ZmORC5. Nucleic Acids Res. 31: 619-628.
43. Wohlschlegel, J.A., Dwyer, B.T., Dhar, S.K., Cvetic, C., Walter, J.C., and Dutta, A. 2000. Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. Science 290: 2309-2312.
44. Wohlschlegel, J.A., Kutok, J.L., Weng, A.P., and Dutta, A. 2002. Expression of geminin as a marker of cell proliferation in normal tissues and malignancies. Am. J. Pathol. 161: 267-273.