Direct analysis of a GPCR-agonist interaction by surface plasmon resonance

European Biophysics Journal

Volumn 35, Issue 8, 2006, Pages 709-712

  Harding, Peter J ^a   Hadingham, Timothy C ^a   McDonnell, James M ^a   Watts, Anthony ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

GPCR; High throughput screening; Ligand binding; Neurotensin receptor; Surface plasmon resonance

Indexed keywords

G PROTEIN COUPLED RECEPTOR; NEUROTENSIN; NEUROTENSIN RECEPTOR; RECOMBINANT PROTEIN;

ARTICLE; BINDING ASSAY; BIOSENSOR; BIOTINYLATION; LIGAND BINDING; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RECEPTOR BINDING; SOLUBILIZATION; SURFACE PLASMON RESONANCE;

ANIMALS; KINETICS; LIGANDS; NEUROTENSIN; RATS; RECEPTORS, NEUROTENSIN; SURFACE PLASMON RESONANCE;

EID: 33749238054     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-006-0070-x     Document Type: Article

References (21)

1. Carraway RE, Leeman SE (1973) Discovery of neurotensin. J Biol Chem 248:6854
2. De Crescenzo G et al (2000) Real-time kinetic studies on the interaction of transforming growth factor R with the epidermal growth factor receptor extracellular domain reveal a conformational change model. Biochemistry 39:9466-9476
3. Grisshammer R et al (1999) Improved purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem Soc 27(5):899-903
4. Heyse S et al (1998) Incorporation of rhodopsin in laterally structured supported membranes: observation of transducin activation with spatially and time-resolved surface plasmon resonance. Biochemistry 37:507-522
5. Iyengar R et al (2002) G-protein pathways. Methods Enzymol 343, 344, 345: Parts A, B and C
6. Ji TH, Ji I (1998) G Protein-coupled receptors. J Biol Chem 28(10):17299-17302
7. Karlsson OP, Lofas S (2002) Flow-mediated on-surface reconstitution of G-protein coupled receptors for applications in surface plasmon resonance biosensors. Anal Biochem 300:132-138
8. Kroger D et al (1999) Ligand binding to nicotinic acetylcholine receptor investigated by surface plasmon resonance. Anal Chem 71:3157
9. McDonnell JM (2001) Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition. Curr Opin Chem Biol 5:572-577
10. Rich RL et al (2002) Kinetic analysis of estrogen receptor/ligand interactions. Proc Natl Acad Sci USA 99(13):8562-8567
11. Sen S et al (2005) Functional studies with membrane-bound and detergent-solubilized a2-adrenergic receptors expressed in Sf 9 cells. Biochim Biophys Acta 1712:62-70
12. Stenlund P et al (2004) Capture and reconstitution of G protein-coupled receptors on a biosensor surface. Anal Biochem 316:243-250
13. Tanaka K et al (1990) Structure and functional expression of the cloned rat neurotensin receptor. Neuron 4(6):847-854
14. Terstappen GC, Reggiani A (2001) In silico research in drug discovery. Trends Pharmacol Sci 22:23-26
15. Tucker J, Grisshammer R (1996) Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem J 317:891-899
16. Van der Merwe PA (2000) Surface plasmon resonance in protein-ligand interactions: a practical approach. Oxford University Press, Oxford
17. Vincent JP et al (1999) Neurotensin and neurotensin receptors. Trends Pharmacol Sci 20:302-309
18. Watts A (2005) Solid state NMR in drug design and discovery for membrane embedded targets. Nat Rev Drug Discov 4:555-568
19. White JF et al (2004) Automated large-scale purification of a G protein-coupled receptor for neurotensin. FEBS Lett 564:289-293
20. Williamson PTF et al (2001) Characterization and assignment of uniformly labeled NT(8-13) at the agonist binding site of the G-protein coupled neurotensin receptor. In: Kühne SR, de Groot HJM (eds) Focus on structural biology, vol 1. Perspectives on solid state NMR in biology. Kluwer, Dordrecht, pp 191-202
21. Williamson PTF et al (2002) Probing the environment of neurotensin whilst bound to the neurotensin receptor by solid state NMR. FEBS Lett 518:111-115