Double site saturation mutagenesis of the human cytochrome P450 2D6 results in regioselective steroid hydroxylation

FEBS Journal

Volumn 280, Issue 13, 2013, Pages 3094-3108

  Geier, Martina ^a   Braun, Andreas ^a   Fladischer, Patrik ^a   Stepniak, Piotr ^b   Rudroff, Florian ^c   Hametner, Christian ^c   Mihovilovic, Marko D ^c   Glieder, Anton ^a,d  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b BIOINFOBANK INSTITUTE   (Poland)

^c VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^d AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

biocatalysis; high throughput Pichia transformation; MuteinDB; Pichia pastoris; protein engineering; regioselectivity; steroid hydroxylation; testosterone; whole cell biotransformation

Indexed keywords

6BETA HYDROXYTESTOSTERONE; CYTOCHROME P450 2D6; STEROID MONOOXYGENASE; TESTOSTERONE; TESTOSTERONE DERIVATIVE; TESTOSTERONE HYDROXYLASE; UNCLASSIFIED DRUG;

BIOTRANSFORMATION; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MUTAGENESIS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; STEROID HYDROXYLATION;

AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 CYP2D6; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROXYLATION; HYDROXYTESTOSTERONES; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUTANT PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TESTOSTERONE;

PICHIA; PICHIA PASTORIS;

EID: 84879198581     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12270     Document Type: Conference Paper

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