Zebularine-induced apoptosis in Calu-6 lung cancer cells is influenced by ROS and GSH level changes

Tumor Biology

Volumn 34, Issue 2, 2013, Pages 1145-1153

  You, Bo Ra ^a   Park, Woo Hyun ^a  

^a Chonbuk National University Medical School   (South Korea)

Author keywords

Apoptosis; Calu 6; DNA methyltransferase; Glutathione; Zebularine

Indexed keywords

ACETYLCYSTEINE; ANTINEOPLASTIC AGENT; BENZYLOXYCARBONYLASPARTYLGLUTAMYLVALYLASPARTYL FLUOROMETHYL KETONE; BENZYLOXYCARBONYLISOLEUCYLGLUTAMYLTHREONYLASPARTYL FLUOROMETHYL KETONE; BENZYLOXYCARBONYLLEUCYLGLUTAMYLHISTIDINYLASPARTYL FLUOROMETHYL KETONE; BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; BUTHIONINE SULFOXIMINE; CASPASE 3; CASPASE 8; GLUTATHIONE; PROTEIN BAX; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG; ZEBULARINE; CASPASE; CYTIDINE; DRUG DERIVATIVE; PYRIMIDIN 2 ONE BETA RIBOFURANOSIDE; PYRIMIDIN-2-ONE BETA-RIBOFURANOSIDE;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CALU 6 LUNG CANCER CELL; CANCER INHIBITION; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL LEVEL; CELL STRAIN; CONCENTRATION RESPONSE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IC 50; LUNG CANCER; OXIDATIVE STRESS; PRIORITY JOURNAL; S PHASE CELL CYCLE CHECKPOINT; CELL CYCLE; CELL PROLIFERATION; DRUG EFFECT; LUNG TUMOR; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; PATHOLOGY; TUMOR CELL CULTURE; WESTERN BLOTTING;

APOPTOSIS; BLOTTING, WESTERN; CASPASES; CELL CYCLE; CELL PROLIFERATION; CYTIDINE; GLUTATHIONE; HUMANS; LUNG NEOPLASMS; MEMBRANE POTENTIAL, MITOCHONDRIAL; REACTIVE OXYGEN SPECIES; TUMOR CELLS, CULTURED;

MLCS; MLOWN;

EID: 84877148468     PISSN: 10104283     EISSN: 14230380     Source Type: Journal    
DOI: 10.1007/s13277-013-0656-8     Document Type: Article

References (37)

1. Lee JY, Lee TH. Effects of DNA methylation on the structure of nucleosomes. J Am Chem Soc. 2012;134:173-5.
2. Gebhard C, Benner C, Ehrich M, Schwarzfischer L, Schilling E, Klug M, et al. General transcription factor binding at cpg islands in normal cells correlates with resistance to de novo DNA methylation in cancer cells. Cancer Res. 2010;70:1398-407.
3. Takeshima H, Yamashita S, Shimazu T, Ushijima T. Effects of genome architecture and epigenetic factors on susceptibility of promoter cpg islands to aberrant DNA methylation induction. Genomics. 2011;98:182-8.
4. Gan L, Chen S, Zhong J, Wang X, Lam EK, Liu X, et al. Zic1 is downregulated through promoter hypermethylation, and functions as a tumor suppressor gene in colorectal cancer. PLoS One. 2011;6:e16916.
5. Lin RK, Hsieh YS, Lin P, Hsu HS, Chen CY, Tang YA, et al. The tobacco-specific carcinogen nnk induces DNA methyltransferase 1 accumulation and tumor suppressor gene hypermethylation in mice and lung cancer patients. J Clin Invest. 2010;120:521-32.
6. Kobayashi Y, Absher DM, Gulzar ZG, Young SR, McKenney JK, Peehl DM, et al. DNA methylation profiling reveals novel biomarkers and important roles for DNA methyltransferases in prostate cancer. Genome Res. 2011;21:1017-27.
7. Weisenberger DJ, Velicescu M, Cheng JC, Gonzales FA, Liang G, Jones PA. Role of the DNA methyltransferase variant dnmt3b3 in DNA methylation. Molecular cancer research: MCR. 2004;2:62-72.
8. Qu Y, Mu G, Wu Y, Dai X, Zhou F, Xu X, et al. Overexpression of DNA methyltransferases 1, 3a, and 3b significantly correlates with retinoblastoma tumorigenesis. Am J Clin Pathol. 2010;134:826-34.
9. Amara K, Ziadi S, Hachana M, Soltani N, Korbi S, Trimeche M. DNA methyltransferase dnmt3b protein overexpression as a prognostic factor in patients with diffuse large b-cell lymphomas. Cancer Sci. 2010;101:1722-30.
10. Gravina GL, Festuccia C, Marampon F, Popov VM, Pestell RG, Zani BM, et al. Biological rationale for the use of DNA methyltransferase inhibitors as new strategy for modulation of tumor response to chemotherapy and radiation. Mol Cancer. 2010;9:305.
11. Lim SP, Neilsen P, Kumar R, Abell A, Callen DF. The application of delivery systems for DNA methyltransferase inhibitors. BioDrugs: clinical immunotherapeutics, biopharmaceuticals and gene therapy. 2011;25:227-42.
12. Gore SD. New ways to use DNA methyltransferase inhibitors for the treatment of myelodysplastic syndrome. Hematology/the Education Program of the American Society of Hematology American Society of Hematology Education Program. 2011;2011:550-5.
13. Flotho C, Claus R, Batz C, Schneider M, Sandrock I, Ihde S, et al. The DNA methyltransferase inhibitors azacitidine, decitabine and zebularine exert differential effects on cancer gene expression in acute myeloid leukemia cells. Leukemia: official journal of the Leukemia Society of America, Leukemia Research Fund, UK. 2009;23:1019-28.
14. Savickiene J, Treigyte G, Jonusiene V, Bruzaite R, Borutinskaite VV, Navakauskiene R. Epigenetic changes by zebularine leading to enhanced differentiation of human promyelocytic leukemia nb4 and kg1 cells. Mol Cell Biochem. 2012;359:245-61.
15. Xiong H, Chen ZF, Liang QC, Du W, Chen HM, Su WY, et al. Inhibition of DNA methyltransferase induces g2 cell cycle arrest and apoptosis in human colorectal cancer cells via inhibition of jak2/stat3/stat5 signalling. J Cell Mol Med. 2009;13:3668-79.
16. Cui M, Wen Z, Chen J, Yang Z, Zhang H. 5-aza-2′-deoxycytidine is a potent inhibitor of DNA methyltransferase 3b and induces apoptosis in human endometrial cancer cell lines with the up-regulation of hmlh1. Med Oncol. 2010;27:278-85.
17. Vivaldi A, Miasaki FY, Ciampi R, Agate L, Collecchi P, Capodanno A, et al. Re-differentiation of thyroid carcinoma cell lines treated with 5-aza-2′-deoxycytidine and retinoic acid. Mol Cell Endocrinol. 2009;307:142-8.
18. Gao S, Mobley A, Miller C, Boklan J, Chandra J. Potentiation of reactive oxygen species is a marker for synergistic cytotoxicity of ms-275 and 5-azacytidine in leukemic cells. Leuk Res. 2008;32:771-80.
19. You BR, Park WH: Zebularine inhibits the growth of hela cervical cancer cells via cell cycle arrest and caspase-dependent apoptosis. Molecular biology reports 2012
20. Bogurcu N, Sevimli-Gur C, Ozmen B, Bedir E, Korkmaz KS. Alcaps induce mitochondrial apoptosis and activate DNA damage response by generating ros and inhibiting topoisomerase i enzyme activity in k562 leukemia cell line. Biochem Biophys Res Commun. 2011;409:738-44.
21. Ravindran J, Gupta N, Agrawal M. Bala Bhaskar AS, Lakshmana Rao PV: Modulation of ros/mapk signaling pathways by okadaic acid leads to cell death via, mitochondrial mediated caspase-dependent mechanism. Apoptosis: an international journal on programmed cell death. 2011;16:145-61.
22. Wagner KW, Ye Y, Lin J, Vaporciyan AA, Roth JA, Wu X. Genetic variations in epigenetic genes are predictors of recurrence in stage i or ii non-small cell lung cancer patients. Clinical cancer research: an official journal of the American Association for Cancer Research. 2012;18:585-92.
23. Feng Q, Hawes SE, Stern JE, Wiens L, Lu H, Dong ZM, et al. DNA methylation in tumor and matched normal tissues from non-small cell lung cancer patients. Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2008;17:645-54.
24. Fulda S, Kufer MU, Meyer E, van Valen F, Dockhorn-Dworniczak B, Debatin KM. Sensitization for death receptor- or drug-induced apoptosis by re-expression of caspase-8 through demethylation or gene transfer. Oncogene. 2001;20:5865-77.
25. Kouidou S, Agidou T, Kyrkou A, Andreou A, Katopodi T, Georgiou E, et al. Non-cpg cytosine methylation of p53 exon 5 in non-small cell lung carcinoma. Lung Cancer. 2005;50:299-307.
26. Billam M, Sobolewski MD, Davidson NE. Effects of a novel DNA methyltransferase inhibitor zebularine on human breast cancer cells. Breast Cancer Res Treat. 2010;120:581-92.
27. Andersen JB, Factor VM, Marquardt JU, Raggi C, Lee YH, Seo D, et al. An integrated genomic and epigenomic approach predicts therapeutic response to zebularine in human liver cancer. Sci Transl Med. 2010;2:54ra77.
28. Park WH, Han YH, Kim SH, Kim SZ. Pyrogallol, ros generator inhibits as4.1 juxtaglomerular cells via cell cycle arrest of g2 phase and apoptosis. Toxicology. 2007;235:130-9.
29. Han YH, Kim SZ, Kim SH, Park WH. Arsenic trioxide inhibits growth of as4.1 juxtaglomerular cells via cell cycle arrest and caspase-independent apoptosis. Am J Physiol Renal Physiol. 2007;293:F511-20.
30. Han YH, Kim SH, Kim SZ, Park WH. Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer calu-6 cells via the prevention of gsh depletion. Int J Oncol. 2008;33:1099-105.
31. You BR, Park WH. Gallic acid-induced lung cancer cell death is related to glutathione depletion as well as reactive oxygen species increase. Toxicology in vitro: an international journal published in association with BIBRA. 2010;24:1356-62.
32. Cheng JC, Weisenberger DJ, Gonzales FA, Liang G, Xu GL, Hu YG, et al. Continuous zebularine treatment effectively sustains demethylation in human bladder cancer cells. Mol Cell Biol. 2004;24:1270-8.
33. Chen M, Shabashvili D, Nawab A, Yang SX, Dyer LM, Brown KD, et al. DNA methyltransferase inhibitor, zebularine, delays tumor growth and induces apoptosis in a genetically engineered mouse model of breast cancer. Mol Cancer Ther. 2012;11:370-82.
34. Bae SI, Cheriyath V, Jacobs BS, Reu FJ, Borden EC. Reversal of methylation silencing of apo2l/trail receptor 1 (dr4) expression overcomes resistance of sk-mel-3 and sk-mel-28 melanoma cells to interferons (ifns) or apo2l/trail. Oncogene. 2008;27:490-8.
35. Li D, Ueta E, Kimura T, Yamamoto T, Osaki T. Reactive oxygen species (ros) control the expression of bcl-2 family proteins by regulating their phosphorylation and ubiquitination. Cancer Sci. 2004;95:644-50.
36. Chaudhari AA, Seol JW, Kim SJ, Lee YJ, Kang HS, Kim IS, et al. Reactive oxygen species regulate bax translocation and mitochondrial transmembrane potential, a possible mechanism for enhanced trail-induced apoptosis by cccp. Oncol Rep. 2007;18:71-6.
37. Schnelldorfer T, Gansauge S, Gansauge F, Schlosser S, Beger HG, Nussler AK. Glutathione depletion causes cell growth inhibition and enhanced apoptosis in pancreatic cancer cells. Cancer. 2000;89:1440-7.