Neurotoxic potential and cellular uptake of T-2 toxin in human astrocytes in primary culture

Chemical Research in Toxicology

Volumn 26, Issue 3, 2013, Pages 347-355

  Weidner, Maria ^a   Lenczyk, Marlies ^a   Schwerdt, Gerald ^b   Gekle, Michael ^b   Humpf, Hans Ulrich ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; HT 2 TOXIN; LACTATE DEHYDROGENASE; LIPOCORTIN 5; T 2 TOXIN;

APOPTOSIS; ARTICLE; ASTROCYTE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG ACCUMULATION; DRUG BINDING; DRUG CYTOTOXICITY; DRUG MECHANISM; DRUG METABOLISM; DRUG UPTAKE; ENZYME ACTIVATION; ENZYME RELEASE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; NERVE CELL CULTURE; NERVE CELL NECROSIS; NEUROTOXICITY; PROTEIN CONTENT;

ANNEXIN A5; APOPTOSIS; ASTROCYTES; CASPASE 3; CELL MEMBRANE PERMEABILITY; CELLS, CULTURED; CEREALS; ENZYME ACTIVATION; FUSARIUM; HT29 CELLS; HUMANS; T-2 TOXIN;

EID: 84875195844     PISSN: 0893228X     EISSN: 15205010     Source Type: Journal    
DOI: 10.1021/tx3004664     Document Type: Article

References (40)

1. Desjardins, A. E., Hohn, T. M., and McCormick, S. P. (1993) Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance Microbiol. Rev. 57, 595-604
2. Sudakin, D. (2003) Trichothecenes in the environment: relevance to human health Toxicol. Lett. 143, 97-107
3. Bouaziz, C., Abidessefi, S., Bouslimi, A., Elgolli, E., and Bacha, H. (2006) Cytotoxicity and related effects of T-2 toxin on cultured Vero cells Toxicon 48, 343-352
4. Edwards, S. G. (2009) Fusarium mycotoxin content of UK organic and conventional oats Food Addit. Contam., Part A 26, 1063-1069
5. Schothorst, R. C. and van Egmond, H. P. (2004) Report from SCOOP task 3.2.10 "collection of occurrence data of Fusarium toxins in food and assessment of dietary intake by the population of EU member states" Toxicol. Lett. 153, 133-143
6. Schwake-Anduschus, C., Langenkämper, G., Unbehend, G., Dietrich, R., Märtlbauer, E., and Münzing, K. (2010) Occurrence of Fusarium T-2 and HT-2 toxins in oats from cultivar studies in Germany and degradation of the toxins during grain cleaning treatment and food processing Food Addit. Contam., Part A 27, 1253-1260
7. van der Fels-Klerx, H., J., and Stratakou, I. (2010) T-2 toxin and HT-2 toxin in grain and grain-based commodities in Europe occurrence, factors affecting occurrence, co-occurrence and toxicological effects World Mycotoxin J. 3, 349-367
8. EFSA Panel on Contaminants in the Food Chain (CONTAM) (2011) Scientific opinion on the risks for animal and public health related to the presence of T-2 and HT-2 toxin in food and feed EFSA J. 12, 2481
9. Wu, Q., Dohnal, V., Huang, L., Kuca, K., and Yuan, Z. (2010) Metabolic pathways of trichothecenes Drug. Metab. Rev. 42, 250-267
10. Johnsen, H., Odden, E., Johnsen, B. A., and Fonnum, F. (1988) Metabolism of T-2 toxin by blood cell carboxylesterases Biochem. Pharmacol. 37, 3193-3197
11. Li, Y., Wang, Z., Beier, R. C., Shen, J., Smet, D. D., De Saeger, S., and Zhang, S. (2011) T-2 Toxin, a trichothecene mycotoxin: Review of toxicity, metabolism, and analytical methods J. Agric. Food Chem. 59, 3441-3453
12. Ohta, M., Ishii, K., and Ueno, Y. (1977) Metabolism of trichothecene mycotoxins. I. Microsomal deacetylation of T-2 toxin in animal tissues J. Biochem. 82, 1591-1598
13. Trusal, L. R. (1986) Metabolism of T-2 mycotoxin by cultured cells Toxicon 24, 597-603
14. Königs, M., Mulac, D., Schwerdt, G., Gekle, M., and Humpf, H.-U. (2009) Metabolism and cytotoxic effects of T-2 toxin and its metabolites on human cells in primary culture Toxicology 258, 106-115
15. Weidner, M., Welsch, T., Hübner, F., Schwerdt, G., Gekle, M., and Humpf, H.-U. (2012) Identification and apoptotic potential of T-2 toxin metabolites in human cells J. Agric. Food Chem. 60, 5676-5684
16. Dugyala, R. R., Kim, Y. W., and Sharma, R. P. (1994) Effects of aflatoxin B1 and T-2 toxin on the granulocyte-macrophage progenitor cells in mouse bone marrow cultures Immunopharmacology 27, 57-65
17. Fairhurst, S., Marrs, T. C., Parker, H. C., Scawin, J. W., and Swanston, D. W. (1987) Acute toxicity of T-2 toxin in rats, mice, guinea-pigs, and pigeons Toxicology 43, 31-49
18. Ueno, Y. (1985) The toxicology of mycotoxins Crit. Rev. Toxicol. 14, 99-132
19. Bennett, J. W. and Klich, M. (2003) Mycotoxins Clin. Microbiol. Rev. 16, 497-516
20. Foroud, N. A. and Eudes, F. (2009) Trichothecenes in cereal grains Int. J. Mol. Sci. 10, 147-173
21. Bouaziz, C., Sharaf El Dein, O., El Golli, E., Abid-Essefi, S., Brenner, C., Lemaire, C., and Bacha, H. (2008) Different apoptotic pathways induced by zearalenone, T-2 toxin and ochratoxin A in human hepatoma cells Toxicology 254, 19-28
22. Chaudhari, M., Jayaraj, R., Bhaskar, A. S. B., and Lakshmana Rao, P. V. (2009) Oxidative stress induction by T-2 toxin causes DNA damage and triggers apoptosis via caspase pathway in human cervical cancer cells Toxicology 262, 153-161
23. Shifrin, V. I. and Anderson, P. (1999) Trichothecene mycotoxins trigger a ribotoxic stress response that activates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase and induces apoptosis J. Biol. Chem. 274, 13985-13992
24. Sirkka, U., Nieminen, S. A., and Ylitalo, P. (1992) Acute neurobehavioural toxicity of trichothecene T-2 toxin in the rat Pharmacol. Toxicol. 70, 111-114
25. Wang, J., Fitzpatrick, D. W., and Wilson, J. R. (1998) Effects of the trichothecene mycotoxin T-2 toxin on neurotransmitters and metabolites in discrete areas of the rat brain Food Chem. Toxicol. 36, 947-953
26. Doi, K. and Uetsuka, K. (2011) Mechanisms of mycotoxin-induced neurotoxicity through oxidative stress-associated pathways Int. J. Mol. Sci. 12, 5213-5237
27. Ravindran, J., Agrawal, M., Gupta, N., and Lakshmana Rao, P. V. (2011) Alteration of blood brain barrier permeability by T-2 toxin: Role of MMP-9 and inflammatory cytokines Toxicology 280, 44-52
28. Königs, M., Schwerdt, G., Gekle, M., and Humpf, H.-U. (2008) Effects of the mycotoxin deoxynivalenol on human primary hepatocytes Mol. Nutr. Food Res. 52, 830-839
29. 3-HT-2 toxin Mycotoxin Res. 25, 41-52
30. Mulac, D. and Humpf, H.-U. (2011) Cytotoxicity and accumulation of ergot alkaloids in human primary cells Toxicology 282, 112-121
31. Königs, M., Lenczyk, M., Schwerdt, G., Holzinger, H., Gekle, M., and Humpf, H.-U. (2007) Cytotoxicity, metabolism and cellular uptake of the mycotoxin deoxynivalenol in human proximal tubule cells and lung fibroblasts in primary culture Toxicology 240, 48-59
32. Bergmeyer, H. U. (1970) Methoden der enzymatischen Analyse, Verlag Chemie, Weinheim, Germany.
33. Vermes, I., Haanen, C., Steffens-Nakken, H., and Reutelingsperger, C. (1995) A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V J. Immunol. Methods 184, 39-51
34. DeLean, A., Munson, P. J., and Rodbard, D. (1978) Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves Am. J. Physiol. 235, E97-102
35. Gutleb, A. C., Morrison, E., and Murk, A. J. (2002) Cytotoxicity assays for mycotoxins produced by Fusarium strains: a review Environ. Toxicol. Pharmacol. 11, 309-320
36. Minervini, F., Fornelli, F., Lucivero, G., Romano, C., and Visconti, A. (2005) T-2 toxin immunotoxicity on human B and T lymphoid cell lines Toxicology 210, 81-91
37. Mikami, O., Yamamoto, S., Yamanaka, N., and Nakajima, Y. (2004) Porcine hepatocyte apoptosis and reduction of albumin secretion induced by deoxynivalenol Toxicology 204, 241-249
38. EU (2001) Opinion of the Scientific Committee on Food on Fusarium Toxins Part 5: T-2 Toxin and HT-2 Toxin, Scientific Committee on Food (SCF), Brussels, Belgium.
39. Welsch, T. and Humpf, H.-U. (2012) HT-2 toxin 4-glucuronide as new T-2 toxin metabolite: enzymatic synthesis, analysis, and species specific formation of T-2 and HT-2 toxin glucuronides by rat, mouse, pig, and human liver microsomes J. Agric. Food Chem. 60, 10170-10178
40. 3H]T-2 toxin Toxicon 27, 1307-1311