The Skin Barrier in Atopic Dermatitis

Harper's Textbook of Pediatric Dermatology: Third Edition

Volumn 1, Issue , 2011, Pages 1-18

  Danby, Simon G ^a   Cork, Michael J ^a,b  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b SHEFFIELD CHILDREN S HOSPITAL   (United Kingdom)

Author keywords

Atopic dermatitis; Desquamation; Differentiation; Filaggrin; Kallikrein; PH; Protease; Stratum corneum; TEWL

Indexed keywords

EID: 84875051926     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781444345384.ch27     Document Type: Chapter

References (150)

1. Cork MJ, Danby SG, Vasilopoulos Y et al. Epidermal barrier dysfunction in atopic dermatitis. J Invest Dermatol 2009; 129 (8): 1892-908.
2. Bieber T. Atopic dermatitis. N Engl J Med 2008; 358 (14): 1483-94.
3. Wood LC, Stalder AK, Liou A et al. Barrier disruption increases gene expression of cytokines and the 55 kD TNF receptor in murine skin. Exp Dermatol 1997; 6 (2): 98-104.
4. Elias PM, Steinhoff M.'Outside-to-inside'(and now back to'outside ') pathogenic mechanisms in atopic dermatitis. J Invest Dermatol 2008; 128 (5): 1067-70.
5. Candi E, Schmidt R, Melino G. The cornifi ed envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol 2005; 6 (4): 328-40.
6. Al-Amoudi A, Frangakis AS. Structural studies on desmosomes. Biochem Soc Trans 2008; 36 (Pt 2): 181-7.
7. Ya-Xian Z, Suetake T, Tagami H. Number of cell layers of the stratum corneum in normal skin-relationship to the anatomical location on the body, age, sex and physical parameters. Arch Dermatol Res 1999; 291 (10): 555-9.
8. Harding CR, Watkinson A, Rawlings AV et al. Dry skin, moisturization and corneodesmolysis. Int J Cosmet Sci 2000; 22 (1): 21-52.
9. Rawlings AV. Trends in stratum corneum research and the management of dry skin conditions. Int J Cosmet Sci 2003; 25 (1-2): 63-95.
10. Norlen L, Al-Amoudi A, Dubochet J. A cryotransmission electron microscopy study of skin barrier formation. J Invest Dermatol 2003; 120 (4): 555-60.
11. Serre G, Mils V, Haftek M et al. Identifi cation of late differentiation antigens of human cornifi ed epithelia, expressed in re-organized desmosomes and bound to cross-linked envelope. J Invest Dermatol 1991; 97 (6): 1061-72.
12. Ishida-Yamamoto A, Simon M, Kishibe M et al. Epidermal lamellar granules transport different cargoes as distinct aggregates. J Invest Dermatol 2004; 122 (5): 1137-44.
13. Caubet C, Jonca N, Brattsand M et al. Degradation of corneodesmosome proteins by two serine proteases of the kallikrein family, SCTE/KLK5/hK5 and SCCE/KLK7/hK7. J Invest Dermatol 2004; 122 (5): 1235-44.
14. Elias PM. Epidermal lipids, barrier function, and desquamation. J Invest Dermatol 1983; 80 Suppl: 44s-9s.
15. Cork MJ, Robinson DA, Vasilopoulos Y et al. New perspectives on epidermal barrier dysfunction in atopic dermatitis: gene-environment interactions. J Allergy Clin Immunol 2006; 118 (1): 3-21; quiz 2-3.
16. Eissa A, Diamandis EP. Human tissue kallikreins as promiscuous modulators of homeostatic skin barrier functions. Biol Chem 2008; 389 (6): 669-80.
17. Bernard D, Mehul B, Thomas-Collignon A et al. Analysis of proteins with caseinolytic activity in a human stratum corneum extract revealed a yet unidentifi ed cysteine protease and identifi ed the so-called "stratum corneum thiol protease" as cathepsin l2. J Invest Dermatol 2003; 120 (4): 592-600.
18. Meyer-Hoffert U. Reddish, scaly, and itchy: how proteases and their inhibitors contribute to infl ammatory skin diseases. Arch Immunol Ther Exp (Warsz) 2009; 57 (5): 345-54.
19. Deraison C, Bonnart C, Lopez F et al. LEKTI fragments specifi cally inhibit KLK5, KLK7, and KLK14 and control desquamation through a pH-dependent interaction. Mol Biol Cell 2007; 18 (9): 3607-19.
20. Kato T, Takai T, Mitsuishi K et al. Cystatin A inhibits IL-8 production by keratinocytes stimulated with Der p 1 and Der f 1: biochemical skin barrier against mite cysteine proteases. J Allergy Clin Immunol 2005; 116 (1): 169-76.
21. Fluhr J, Bankova LG. Skin surface pH: mechanism, measurement, importance. In: Serup J, Jemec GB, Grove GL (eds) Handbook of Non-Invasive Methods and the Skin. Boca Raton, FL: CRC Press, 2006, pp. 411-20.
22. Nakagawa N, Sakai S, Matsumoto M et al. Relationship between NMF (lactate and potassium) content and the physical properties of the stratum corneum in healthy subjects. J Invest Dermatol 2004; 122 (3): 755-63.
23. Houben E, Hachem JP, de Paepe K et al. Epidermal ceramidase activity regulates epidermal desquamation via stratum corneum acidifi cation. Skin Pharmacol Physiol 2008; 21 (2): 111-18.
24. Behne MJ, Meyer JW, Hanson KM et al. NHE1 regulates the stratum corneum permeability barrier homeostasis. Microenvironment acidifi cation assessed with fl uorescence lifetime imaging. J Biol Chem 2002; 277 (49): 47399-406.
25. Fluhr JW, Kao J, Jain M et al. Generation of free fatty acids from phospholipids regulates stratum corneum acidifi cation and integrity. J Invest Dermatol 2001; 117 (1): 44-51.
26. Mauro T, Holleran WM, Grayson S et al. Barrier recovery is impeded at neutral pH, independent of ionic effects: implications for extracellular lipid processing. Arch Dermatol Res 1998; 290 (4): 215-22.
27. Hachem JP, Man MQ, Crumrine D et al. Sustained serine proteases activity by prolonged increase in pH leads to degradation of lipid processing enzymes and profound alterations of barrier function and stratum corneum integrity. J Invest Dermatol 2005; 125 (3): 510-20.
28. Elias P, Ahn S, Brown B et al. Origin of the epidermal calcium gradient: regulation by barrier status and role of active vs passive mechanisms. J Invest Dermatol 2002; 119 (6): 1269-74.
29. Forslind B, Werner-Linde Y, Lindberg M et al. Elemental analysis mirrors epidermal differentiation. Acta Derm Venereol 1999; 79 (1): 12-7.
30. Elias PM, Ahn SK, Denda M et al. Modulations in epidermal calcium regulate the expression of differentiation-specifi c markers. J Invest Dermatol 2002; 119 (5): 1128-36.
31. Lee SH, Elias PM, Proksch E et al. Calcium and potassium are important regulators of barrier homeostasis in murine epidermis. J Clin Invest 1992; 89 (2): 530-8.
32. Hachem JP, Houben E, Crumrine D et al. Serine protease signaling of epidermal permeability barrier homeostasis. J Invest Dermatol 2006; 126 (9): 2074-86.
33. Stefansson K, Brattsand M, Roosterman D et al. Activation of proteinase-activated receptor-2 by human kallikrein-related peptidases. J Invest Dermatol 2008; 128 (1): 18-25.
34. Ramachandran R, Hollenberg MD. Proteinases and signalling: pathophysiological and therapeutic implications via PARs and more. Br J Pharmacol 2008; 153 Suppl 1: S263-82.
35. Briot A, Deraison C, Lacroix M et al. Kallikrein 5 induces atopic dermatitis-like lesions through PAR2-mediated thymic stromal lymphopoietin expression in Netherton syndrome. J Exp Med 2009; 206 (5): 1135-47.
36. He R, Geha RS. Thymic stromal lymphopoietin. Ann N Y Acad Sci 2010; 1183: 13-24.
37. Yoo J, Omori M, Gyarmati D et al. Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifi cally in the skin. J Exp Med 2005; 202 (4): 541-9.
38. Demerjian M, Hachem JP, Tschachler E et al. Acute modulations in permeability barrier function regulate epidermal cornifi cation: role of caspase-14 and the protease-activated receptor type 2. Am J Pathol 2008; 172 (1): 86-97.
39. Macfarlane SR, Sloss CM, Cameron P et al. The role of intracellular Ca2+ in the regulation of proteinase-activated receptor-2 mediated nuclear factor kappa B signalling in keratinocytes. Br J Pharmacol 2005; 145 (4): 535-44.
40. Hachem JP, Man MQ, Crumrine D et al. Sustained serine proteases activity by prolonged increase in pH leads to degradation of lipid processing enzymes and profound alterations of barrier function and stratum corneum integrity. J Invest Dermatol 2005; 125 (3): 510-20.
41. Katsuta Y, Iida T, Hasegawa K et al. Function of oleic acid on epidermal barrier and calcium infl ux into keratinocytes is associated with N-methyl D-aspartate-type glutamate receptors. Br J Dermatol 2009; 160 (1): 69-74.
42. Darmstadt GL, Mao-Qiang M, Chi E et al. Impact of topical oils on the skin barrier: possible implications for neonatal health in developing countries. Acta Paediatr 2002; 91 (5): 546-54.
43. Hanley K, Jiang Y, Crumrine D et al. Activators of the nuclear hormone receptors PPARalpha and FXR accelerate the development of the fetal epidermal permeability barrier. J Clin Invest 1997; 100 (3): 705-12.
44. Cork MJ, Danby SG, Vasilopoulos Y et al. Epidermal barrier dysfunction in atopic dermatitis. J Invest Dermatol 2009; 129 (8): 1892-908.
45. Mucke H, Mohr KT, Rummler A et al. [Skin pH value on hands after application of soap, cleaners and hand disinfectants]. Pharmazie 1993; 48 (6): 468-9.
46. Voegeli D. The effect of washing and drying practices on skin barrier function. J Wound Ostomy Continence Nurs 2008; 35 (1): 84-90.
47. White MI, Jenkinson DM, Lloyd DH. The effect of washing on the thickness of the stratum corneum in normal and atopic individuals. Br J Dermatol 1987; 116 (4): 525-30.
48. Hirasawa Y, Takai T, Nakamura T et al. Staphylococcus aureus extracellular protease causes epidermal barrier dysfunction. J Invest Dermatol 2010; 130 (2): 614-17.
49. Leyden JJ, Marples RR, Kligman AM. Staphylococcus aureus in the lesions of atopic dermatitis. Br J Dermatol 1974; 90 (5): 525-30.
50. Jeong SK, Kim HJ, Youm JK et al. Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery. J Invest Dermatol 2008; 128 (8): 1930-9.
51. Kunz B, Ring J. Clinical features and diagnostic criteria of atopic dermatitis. In: Harper JI, Oranje A, Prose N (eds) Textbook of Pediatric Dermatology. Oxford: Blackwell Science, 2002.
52. Dotterud LK, Kvammen B, Lund E et al. Prevalence and some clinical aspects of atopic dermatitis in the community of Sor-Varanger. Acta Derm Venereol 1995; 75 (1): 50-3.
53. Schudel P, Wuthrich B. [Clinical course of childhood atopic neurodermatitis. A catamnestic study of 121 cases]. Z Hautkr 1985; 60 (6): 479-86.
54. Lee Y, Hwang K. Skin thickness of Korean adults. Surg Radiol Anat 2002; 24 (3-4): 183-9.
55. Barker DE. Skin thickness in the human. Plast Reconstr Surg 1951; 7 (2): 115-16.
56. Southwood WF. The thickness of the skin. Plast Reconstr Surg 1955; 15 (5): 423-9.
57. Ya-Xian Z, Suetake T, Tagami H. Number of cell layers of the stratum corneum in normal skin-relationship to the anatomical location on the body, age, sex and physical parameters. Arch Dermatol Res 1999; 291 (10): 555-9.
58. Feldmann RJ, Maibach HI. Regional variation in percutaneous penetration of 14C cortisol in man. J Invest Dermatol 1967; 48 (2): 181-3.
59. Marzulli FN. Barriers to skin penetration. J Invest Dermatol 1962; 39: 387-93.
60. Schaefer KE, Scheer K. Regional differences in CO2 elimination through the skin. Exp Med Surg 1951; 9 (2-4): 449-57.
61. Cronin E, Stoughton RB. Percutaneous absorption of nicotinic acid and ethyl nicotinate in human skin. Nature 1962; 195: 1103-4.
62. Kashibuchi N, Hirai Y, O'Goshi K et al. Three-dimensional analyses of individual corneocytes with atomic force microscope: morphological changes related to age, location and to the pathologic skin conditions. Skin Res Technol 2002; 8 (4): 203-11.
63. Rougier A, Lotte C, Corcuff TP et al. Relationship between skin permeability and corneocyte size according to anatomic site, age and sex in a man. J Soc Cosmet Chem 1988; 39: 15-26.
64. Holzle E, Plewig G. Effects of dermatitis, stripping, and steroids on the morphology of corneocytes. A new bioassay. J Invest Dermatol 1977; 68 (6): 350-6.
65. Voegeli R, Rawlings AV, Doppler S et al. Profi ling of serine protease activities in human stratum corneum and detection of a stratum corneum tryptase-like enzyme. Int J Cosmet Sci 2007; 29 (3): 191-200.
66. Fluhr J, Bankova LG. Skin surface pH: mechanism, measurement, importance. In: Serup J, Jemec GB, Grove GL (eds) Handbook of Non-Invasive Methods and the Skin. Boca Raton, FL: CRC Press, 2006, pp. 411-20.
67. Nikolovski J, Stamatas GN, Kollias N et al. Barrier function and water-holding and transport properties of infant stratum corneum are different from adult and continue to develop through the fi rst year of life. J Invest Dermatol 2008; 128 (7): 1728-36.
68. Voegeli R, Rawlings AV, Doppler S et al. Increased basal transepidermal water loss leads to elevation of some but not all stratum corneum serine proteases. Int J Cosmet Sci 2008; 30 (6): 435-42.
69. Palmer CN, Irvine AD, Terron-Kwiatkowski A et al. Common loss-of-function variants of the epidermal barrier protein fi laggrin are a major predisposing factor for atopic dermatitis. Nat Genet 2006; 38 (4): 441-6.
70. Brown SJ, McLean WH. Eczema genetics: current state of knowledge and future goals. J Invest Dermatol 2009; 129 (3): 543-52.
71. Saaf AM, Tengvall-Linder M, Chang HY et al. Global expression profi ling in atopic eczema reveals reciprocal expression of infl ammatory and lipid genes. PLoS ONE 2008; 3 (12): e4017.
72. Guttman-Yassky E, Suarez-Farinas M, Chiricozzi A et al. Broad defects in epidermal cornifi cation in atopic dermatitis identifi ed through genomic analysis. J Allergy Clin Immunol 2009; 124 (6): 1235-44 e58.
73. Sugiura H, Ebise H, Tazawa T et al. Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornifi ed envelope. Br J Dermatol 2005; 152 (1): 146-9.
74. Kezic S, Kemperman PM, Koster ES et al. Loss-of-function mutations in the fi laggrin gene lead to reduced level of natural moisturizing factor in the stratum corneum. J Invest Dermatol 2008; 128 (8): 2117-19.
75. Harding CR, Watkinson A, Rawlings AV et al. Dry skin, moisturization and corneodesmolysis. Int J Cosmet Sci 2000; 22 (1): 21-52.
76. Nakagawa N, Sakai S, Matsumoto M et al. Relationship between NMF (lactate and potassium) content and the physical properties of the stratum corneum in healthy subjects. J Invest Dermatol 2004; 122 (3): 755-63.
77. Schurer NY, Elias PM. The biochemistry and function of stratum corneum lipids. Adv Lipid Res 1991; 24: 27-56.
78. Proksch E, Jensen JM, Elias PM. Skin lipids and epidermal differentiation in atopic dermatitis. Clin Dermatol 2003; 21 (2): 134-44.
79. Meguro S, Arai Y, Masukawa Y et al. Relationship between covalently bound ceramides and transepidermal water loss (TEWL). Arch Dermatol Res 2000; 292 (9): 463-8.
80. Schafer L, Kragballe K. Abnormalities in epidermal lipid metabolism in patients with atopic dermatitis. J Invest Dermatol 1991; 96 (1): 10-15.
81. Katsuta Y, Iida T, Hasegawa K et al. Function of oleic acid on epidermal barrier and calcium infl ux into keratinocytes is associated with N-methyl D-aspartate-type glutamate receptors. Br J Dermatol 2009; 160 (1): 69-74.
82. Fluhr J, Bankova LG. Skin surface pH: mechanism, measurement, importance. In: Serup J, Jemec GB, Grove GL (eds) Handbook of Non-Invasive Methods and the Skin. Boca Raton, FL: CRC Press, 2006, pp. 411-20.
83. Hachem JP, Man MQ, Crumrine D et al. Sustained serine proteases activity by prolonged increase in pH leads to degradation of lipid processing enzymes and profound alterations of barrier function and stratum corneum integrity. J Invest Dermatol 2005; 125 (3): 510-20.
84. Hatano Y, Man MQ, Uchida Y et al. Maintenance of an acidic stratum corneum prevents emergence of murine atopic dermatitis. J Invest Dermatol 2009; 129 (7): 1824-35.
85. Demerjian M, Hachem JP, Tschachler E et al. Acute modulations in permeability barrier function regulate epidermal cornifi cation: role of caspase-14 and the protease-activated receptor type 2. Am J Pathol 2008; 172 (1): 86-97.
86. Ramachandran R, Hollenberg MD. Proteinases and signalling: pathophysiological and therapeutic implications via PARs and more. Br J Pharmacol 2008; 153 Suppl 1: S263-82.
87. Komatsu N, Saijoh K, Kuk C et al. Human tissue kallikrein expression in the stratum corneum and serum of atopic dermatitis patients. Exp Dermatol 2007; 16 (6): 513-19.
88. Voegeli R, Rawlings AV, Breternitz M et al. Increased stratum corneum serine protease activity in acute eczematous atopic skin. Br J Dermatol 2009; 161 (1): 70-7.
89. Hansson L, Backman A, Ny A et al. Epidermal overexpression of stratum corneum chymotryptic enzyme in mice: a model for chronic itchy dermatitis. J Invest Dermatol 2002; 118 (3): 444-9.
90. Vasilopoulos Y, Cork MJ, Murphy R et al. Genetic association between an AACC insertion in the 3'UTR of the stratum corneum chymotryptic enzyme gene and atopic dermatitis. J Invest Dermatol 2002; 123 (1): 62-6.
91. Weidinger S, Baurecht H, Wagenpfeil S et al. Analysis of the individual and aggregate genetic contributions of previously identifi ed serine peptidase inhibitor Kazal type 5 (SPINK5), kallikrein-related peptidase 7 (KLK7), and fi laggrin (FLG) polymorphisms to eczema risk. J Allergy Clin Immunol 2008; 122 (3): 560-8 e4.
92. Voegeli R, Rawlings AV, Doppler S et al. Increased basal transepidermal water loss leads to elevation of some but not all stratum corneum serine proteases. Int J Cosmet Sci 2008; 30 (6): 435-42.
93. Stefansson K, Brattsand M, Roosterman D et al. Activation of proteinase-activated receptor-2 by human kallikrein-related peptidases. J Invest Dermatol 2008; 128 (1): 18-25.
94. Soumelis V, Reche PA, Kanzler H et al. Human epithelial cells trigger dendritic cell mediated allergic infl ammation by producing TSLP. Nat Immunol 2002; 3 (7): 673-80.
95. Walley AJ, Chavanas S, Moffatt MF et al. Gene polymorphism in Netherton and common atopic disease. Nat Genet 2001; 29 (2): 175-8.
96. Morar N, Willis-Owen SA, Moffatt MF et al. The genetics of atopic dermatitis. J Allergy Clin Immunol 2006; 118 (1): 24-34.
97. Briot A, Deraison C, Lacroix M et al. Kallikrein 5 induces atopic dermatitis-like lesions through PAR2-mediated thymic stromal lymphopoietin expression in Netherton syndrome. J Exp Med 2009; 206 (5): 1135-47.
98. Vasilopoulos Y, Cork MJ, Teare D et al. A nonsynonymous substitution of cystatin A, a cysteine protease inhibitor of house dust mite protease, leads to decreased mRNA stability and shows a signifi cant association with atopic dermatitis. Allergy 2007; 62 (5): 514-19.
99. Spergel JM, Paller AS. Atopic dermatitis and the atopic march. J Allergy Clin Immunol 2003; 112 (6 Suppl): S118-27.
100. Chiou YB, Blume-Peytavi U. Stratum corneum maturation. A review of neonatal skin function. Skin Pharmacol Physiol 2004; 17 (2): 57-66.
101. Nuutinen J. Measurement of transepidermal water loss by closed-chamber systems. In: Serup J, Jemec GBE, Grove GL (eds) Handbook of Non-Invasive Methods And The Skin, 2nd edn. Boca Raton, FL: CRC Press, 2006, pp. 393-6.
102. Nikolovski J, Stamatas GN, Kollias N et al. Barrier function and water-holding and transport properties of infant stratum corneum are different from adult and continue to develop through the fi rst year of life. J Invest Dermatol 2008; 128 (7): 1728-36.
103. Fluhr JW, Mao-Qiang M, Brown BE et al. Functional consequences of a neutral pH in neonatal rat stratum corneum. J Invest Dermatol 2004; 123 (1): 140-51.
104. Levin J, Maibach H. The correlation between transepidermal water loss and percutaneous absorption: an overview. J Control Release 2005; 103 (2): 291-9.
105. West DP, Halket JM, Harvey DR et al. Percutaneous absorption in preterm infants. Pediatr Dermatol 1987; 4 (3): 234-7.
106. Behne MJ, Barry NP, Hanson KM et al. Neonatal development of the stratum corneum pH gradient: localization and mechanisms leading to emergence of optimal barrier function. J Invest Dermatol 2003; 120 (6): 998-1006.
107. Stamatas GN, Nikolovski J, Luedtke MA et al. Infant skin microstructure assessed in vivo differs from adult skin in organization and at the cellular level. Pediatr Dermatol 2010; 27 (2): 125-31.
108. Nakagawa N, Sakai S, Matsumoto M et al. Relationship between NMF (lactate and potassium) content and the physical properties of the stratum corneum in healthy subjects. J Invest Dermatol 2004; 122 (3): 755-63.
109. Fluhr JW, Man MQ, Hachem JP et al. Topical peroxisome proliferator activated receptor activators accelerate postnatal stratum corneum acidifi cation. J Invest Dermatol 2009; 129 (2): 365-74.
110. Ramasastry P, Downing DT, Pochi PE et al. Chemical composition of human skin surface lipids from birth to puberty. J Invest Dermatol 1970; 54 (2): 139-44.
111. Hanley K, Jiang Y, He SS et al. Keratinocyte differentiation is stimulated by activators of the nuclear hormone receptor PPARalpha. J Invest Dermatol 1998; 110 (4): 368-75.
112. Wertz PW, Downing DT. Linoleate content of epidermal acylglucosylceramide in newborn, growing and mature mice. Biochim Biophys Acta 1986; 876 (3): 469-73.
113. Drake DR, Brogden KA, Dawson DV et al. Thematic review series: skin lipids. Antimicrobial lipids at the skin surface. J Lipid Res 2008; 49 (1): 4-11.
114. Basta M, Wilburg J, Heczko PB. In vitro effects of skin lipid extracts on skin bacteria in relation to age and acne changes. J Invest Dermatol 1980; 74 (6): 437-9.
115. Fluhr J, Bankova LG. Skin surface pH: mechanism, measurement, importance. In: Serup J, Jemec GBE, Grove GL (eds) Handbook of Non-Invasive Methods and the Skin, 2nd edn. Boca Raton, FL: CRC Press, 2006, pp. 411-20.
116. Hirasawa Y, Takai T, Nakamura T et al. Staphylococcus aureus extracellular protease causes epidermal barrier dysfunction. J Invest Dermatol 2010; 130 (2): 614-17.
117. Leyden JJ, Marples RR, Kligman AM. Staphylococcus aureus in the lesions of atopic dermatitis. Br J Dermatol 1974; 90 (5): 525-30.
118. Huang JT, Abrams M, Tlougan B et al. Treatment of Staphylococcus aureus colonization in atopic dermatitis decreases disease severity. Pediatrics 2009; 123 (5): e808-14.
119. Bieber T. Atopic dermatitis. N Engl J Med 2008; 358 (14): 1483-94.
120. Illi S, von Mutius E, Lau S et al. The natural course of atopic dermatitis from birth to age 7 years and the association with asthma. J Allergy Clin Immunol 2004; 113 (5): 925-31.
121. Lee Y, Hwang K. Skin thickness of Korean adults. Surg Radiol Anat 2002; 24 (3-4): 183-9.
122. White MI, Jenkinson DM, Lloyd DH. The effect of washing on the thickness of the stratum corneum in normal and atopic individuals. Br J Dermatol 1987; 116 (4): 525-30.
123. Paller AS, Mancini A. Hurwitz Clinical Pediatric Dermatology, 3rd edn. Phildelphia: Saunders, 2006.
124. Siqueira WL, Bermejo PR, Mustacchi Z et al. Buffer capacity, pH, and fl ow rate in saliva of children aged 2-60 months with Down syndrome. Clin Oral Invest 2005; 9 (1): 26-9.
125. Harrison VC, Peat G. Signifi cance of milk pH in newborn infants. BMJ 1972; 4 (5839): 515-18.
126. Ireson NJ, Tait JS, MacGregor GA et al. Comparison of nasal pH values in black and white individuals with normal and high blood pressure. Clin Sci (Lond) 2001; 100 (3): 327-33.
127. Adam R. Skin care of the diaper area. Pediatr Dermatol 2008; 25 (4): 427-33.
128. Lack G, Fox D, Northstone K et al. Factors associated with the development of peanut allergy in childhood. N Engl J Med 2003; 348 (11): 977-85.
129. Lewis-Jones S, Cork MJ, Clark C et al. Atopic eczema in children-guideline consultation: a systematic review of the treatments for atopic eczema and guideline for its management [electronic article]. London: National Institute for Clinical Excellence (NICE), Department of Health, 2007. Available from: http://guidance.nice.org.uk/page.aspx?o= 434713.
130. Kuehl BL, Fyfe KS, Shear NH. Cutaneous cleansers. Skin Therapy Lett 2003; 8 (3): 1-4.
131. Cork MJ, Timmins J, Holden C et al. An audit of adverse drug reactions to aqueous cream in children with atopic eczema. Pharmaceut J 2003; 271: 746-7.
132. Al Enezi T, Sultan A, Chittock J et al. Breakdown of the skin barrier induced by aqueous cream: implications for the management of atopic eczema. 89th Annual Meeting of the British Association of Dermatologists, Glasgow, 2009.
133. Tsai TF, Maibach HI. How irritant is water?. An overview. Contact Dermatitis 1999; 41 (6): 311-14.
134. Warren R, Ertel KD, Bartolo RG et al. The infl uence of hard water (calcium) and surfactants on irritant contact dermatitis. Contact Dermatitis 1996; 35 (6): 337-43.
135. McNally NJ, Williams HC, Phillips DR et al. Atopic eczema and domestic water hardness. Lancet 1998; 352 (9127): 527-31.
136. Darmstadt GL, Mao-Qiang M, Chi E et al. Impact of topical oils on the skin barrier: possible implications for neonatal health in developing countries. Acta Paediatr 2002; 91 (5): 546-54.
137. Katsuta Y, Iida T, Hasegawa K et al. Function of oleic acid on epidermal barrier and calcium infl ux into keratinocytes is associated with N-methyl D-aspartate-type glutamate receptors. Br J Dermatol 2009; 160 (1): 69-74.
138. Hanley K, Jiang Y, Crumrine D et al. Activators of the nuclear hormone receptors PPARalpha and FXR accelerate the development of the fetal epidermal permeability barrier. J Clin Invest 1997; 100 (3): 705-12.
139. Proksch E, Jensen JM, Elias PM. Skin lipids and epidermal differentiation in atopic dermatitis. Clin Dermatol 2003; 21 (2): 134-44.
140. Lee SH, Elias PM, Proksch E et al. Calcium and potassium are important regulators of barrier homeostasis in murine epidermis. J Clin Invest 1992; 89 (2): 530-8.
141. Naik A, Pechtold LARM, Potts RO et al. Mechanism of oleic acid-induced skin penetration enhancement in vivo in humans. J Control Release 1995; 37 (3): 299-306.
142. Brown SJ, McLean WH. Eczema genetics: current state of knowledge and future goals. J Invest Dermatol 2009; 129 (3): 543-52.
143. Cork MJ, Danby SG, Vasilopoulos Y et al. Epidermal barrier dysfunction in atopic dermatitis. J Invest Dermatol 2009; 129 (8): 1892-908.
144. Brown SJ, McLean WH. Eczema genetics: current state of knowledge and future goals. J Invest Dermatol 2009; 129 (3): 543-52.
145. Morar N, Willis-Owen SA, Moffatt MF et al. The genetics of atopic dermatitis. J Allergy Clin Immunol 2006; 118 (1): 24-34.
146. Fluhr J, Bankova LG. Skin surface pH: mechanism, measurement, importance. In: Serup J, Jemec GB, Grove GL (eds) Handbook of Non-Invasive Methods and the Skin. Boca Raton, FL: CRC Press, 2006, pp. 411-20.
147. Hachem JP, Man MQ, Crumrine D et al. Sustained serine proteases activity by prolonged increase in pH leads to degradation of lipid processing enzymes and profound alterations of barrier function and stratum corneum integrity. J Invest Dermatol 2005; 125 (3): 510-20.
148. Briot A, Deraison C, Lacroix M et al. Kallikrein 5 induces atopic dermatitis-like lesions through PAR2-mediated thymic stromal lymphopoietin expression in Netherton syndrome. J Exp Med 2009; 206 (5): 1135-47.
149. Demehri S, Morimoto M, Holtzman MJ et al. Skin-derived TSLP triggers progression from epidermal barrier defects to asthma. PLoS Biol 2009; 7 (5): e1000067.
150. Bieber T. Atopic dermatitis. N Engl J Med 2008; 358 (14): 1483-94.