Sample-to-result STR genotyping systems: Potential and status

Forensic Science Review

Volumn 24, Issue 2, 2012, Pages 123-142

  Lounsbury, J A ^a   Bienvenue, J M ^a   Landers, J P ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

DNA extraction; Micro total analysis system; Microchip electrophoresis; PCR; Rapid ID; STR typing

Indexed keywords

EID: 84874869688     PISSN: 10427201     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (119)

1. Belder D, Ludwig M: Surface modification in microchip electrophoresis; Electrophoresis 24:3595; 2003.
2. Belgrader P, Young S, Yuan B., Primeau M, Christel LA, Pourahmadi F, Northrup M A: A battery-powered notebook thermal cycler for rapid multiplex real time PCR analysis; Anal Chem 73:286; 2001.
3. Bessetti J: An introduction to PCR inhibitors; Profiles in DNA 10:9; 2007.
4. Bienvenue JM, Duncalf N, Marchiarullo D., Ferrance JP, Landers JP: Microchip-based cell lysis and DNA extraction from sperm cells for application to forensic analysis; J Forensic Sci 51:266; 2006.
5. Bienvenue JM, Giles R, Landers J.P., Bell S: Integrated microfluidics for forensic applications; Presentation - 61st Annual Meeting of the American Academy of Forensic Sciences; Denver, CO; February 16-21; 2009.
6. Bienvenue JM. Legendre LA, Ferrance JP, Landers JP: An integrated microfluidic device for DNA purification and PCR amplification of STR fragments; Forensic Sci Int Genet 4:178; 2010.
7. Bienvenue JM, Wilson KL, Landers J.P., Ferrance JP: Evaluation of sieving polymers for fast, reproducible electrophoretic analysis of short tandem repeats (STR) in capillaries; J Forensic Sci 50:2005.
8. Breadmore MC, Wolfe KA, Arcibal I.G., Leung WK, Dickson D, Giordano BC, Power ME, Ferrance JP, Feldman SH, Norris PM, Landers JP: Microchip-based purification of DNA from biological samples; Anal Chem 75:1880; 2003.
9. Brevnov MG, Pawar HS, Mundt J, Calandro L.M., Furtado MR, Shewale JG: Developmental validation of the PrepFiler™ forensic DNA extraction kit for extraction of genomic DNA from biological samples; J Forensic Sci 54:599; 2009.
10. Butler JM: Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers; Elsevier Academic Press: Burlington, MA; 2005.
11. Butler JM, Buel E, Crivellente F., McCord BR: Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis; Electrophoresis 25:1397; 2004.
12. Callaghan T: Rapid DNA Analysis in the Police Booking Suite: "FBI Inititative for Reference Sample Point of Collection Analysis,"; presentation - 22nd International Symposium on Human Identification; National Harbor, MD; October 3-6, 2011.
13. Chen L, Manz A, Day PJR: Total nucleic acid analysis integrated on microfluidic devices; Lab on a Chip 7:1413; 2007.
14. Chen X, Cui DF: Microfluidic devices for sample pretreat-ment and applications; Microsyst Technol 15:667; 2009.
15. Christel LA, Petersen K, McMillan W., Northrup MA: Rapid, automated nucleic acid probe assays using silicon microstructures for nucleic acid concentration; J Biomech Eng-TASME 121:22; 1999.
16. Defining, counting and reducing the casework backlog; In The DNA Initiative; http://www.dna.gov (accessed August 12, 2011).
17. do Lago C.L., da Silva HDT, Neves CA, Brito-Neto JGA, da Silva JAF: A dry process for production of microfluidic devices based on the lamination of laser-printed polyester films; Anal Chem 75:2003.
18. Duarte GRM, Price CW, Augustine B.H., Carrilho E., Landers JP: Dynamic solid phase DNA extraction and PCR amplification in polyester-toner based microchip; Anal Chem 83:5182; 2011.
19. Duarte GRM, Price CW, Littlewood J.L., Haverstick DM, Ferrance JP, Carrilho E, Landers JP: Characterization of dynamic solid phase DNA extraction from blood with magnetically controlled silica beads; Analyst 135:531; 2010.
20. Easley CJ, Humphrey JAC, Landers JP: Thermal isolation of microchip reaction chambers for rapid non-contact DNA amplification; J Micromech Microeng 17:1758; 2007.
21. Easley CJ, Karlinsey JM, Bienvenue J.M., Legendre LA, Roper MG, Feldman SH, Hughes M.A., Hewlett EL, Merkel TJ, Ferrance JP, et al.: A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability; Proc Natl Acad Sci USA 103:19272; 2006.
22. Easley CJ, Karlinsey JM, Landers JP: On-chip pressure injection for integration of infrared-mediated DNA amplification with electrophoretic separation; Lab on a Chip 6:2006.
23. Edwards A, Civitello A, Hammond H.A., Caskey CT: DNA typing and geretic-mapping with trimeric and tetrameric tandem repeats; Am J Hum Genet 49:746; 1991.
24. Edwards A, Hammond HA, Jin L, Caskey C.T., Chakraborty R: Genetic-variation at 5 trimeric and tetrameric tandem repeat loci in 4 human-population groups; Genomics 12:241; 1992.
25. El-Sissi O., Franklin H, Nielsen B., Pagano S, Belcinski R, Bogdan G., Jovanovich S: RapidHIT 200 human DNA identification system; http://integenx.com/ applications-2/dna-identity/ (accessed March 5, 2012).
26. Epstein D, Grates K: Evaluating rugged and portable chemical testing platforms; Presentation - 25 th National Environmental Monitorir, g Conference; San Antonio, TX; August 10-14, 2009.
27. Felhofer JL, Blanes L, Gacia CD: Recent developments in instrumentation for capillary electrophoresis and microchip-capillary electrophoresis; Electrophoresis 31:2469; 2010.
28. Forster RE, Chiesl TN, Fredlake C.P., White CV, Barron AE: Hydrophpbically modified Polyacrylamide block copolymers for fast, high-resolution DNA sequencing in microfluidic chips; Electrophoresis 29:4669; 2008.
29. Fredlake CP, Hert DG, Can C.W., Chiesl TN, Root BE, Forster RE, Barron AE: Ultrafast DNA sequencing on a microchip by a hybrid separation mechanism that gives 600 bases in 6.5 minutes; Proc Natl Acad Sci USA 105:476; 2008.
30. Fuhr G, Muller T, Bauklph V., Lucas K: High-frequency electric field trapping of individual human spermatozoa; Hum Reprod 13:136; 1998.
31. Fujita Y, Kubo S: Application of FTA technology to extraction of sperm DNA from mixed body fluids containing semen; Leg Med (Tokyo) 8:43; 2006.
32. Giddings MC, Severin J, Westphall M., Wu J, Smith LM: A software system for data analysis in automated DNA sequencing; Genom Res 5:644; 1998.
33. Giese H, Lam R, Seiden R., Tan E: Fast multiplexed polymerase chain reaction for conventional and microfluidic short tandem repea analysis; J Forensic Sci 54:1287; 2009.
34. Gill P, Jeffreys AJ, Werrett DJ: Forensic application of DNA fingerprints; Nature 318:577; 1985.
35. Goedecke N, j McKenna B, El-Difrawy S, Carey L., Matsudaira P, Ehrlich D: A high-performance multilane microdevice system designed for the DNA forensic laboratory; Electrophoresis 25:1678; 2004.
36. Goedecke N, McKenna B, El-Difrawy S, Gismondi E., Swenson A, Carey L, MJatsudaira P., Ehrlich DJ: Microfluidic DNA forensics by the simple tandem repeat method; J Chromatogr A 1111:206; 2006.
37. Greenspoon SA, Yeung SHI, Johnson KR, Chu W.K., Rhee HN, McGuckian AB, Crouse CA, Chiesl TN, Barron AE, Scherer JR, et al.: A forensic laboratory tests the Berkeley microfabricated capillary array electrophoresis device; J Forensic Sci 53:828; 2008.
38. Grover WH, Skelley AM, Liu C.N., Lagally ET, Mathies RA: Monolith c membrane valves and diaphragm pumps for practical la ge-scale integration into glass microfluidic devices; Sensor Actuat B-Chem 89:315; 2003.
39. Grunenwald H: Optimization of polymerase chain reactions; In Bartlett JM, Stirling D (Eds): PCR Protocols, 2nd ed; Humana Press: Totowa, NJ; p 89; 2003.
40. Hagan KA, Reedy CR, Bienvenue J.M., Dewald AH, Landers JP: A valveless microfluidic device for integrated solid phase extraction and polymerase chain reaction for short tandem repeat (STR) analysis; Analyst 136:2011.
41. Hammond HA, Edwards A, Jin L., Chakraborty R, Caskey CT: Studies of multilocus genotype data validate the use of DNA typing with polymorphic trimeric and tetrameric tandem repeats for personal identification; Am J Hum Genet 49:441; 1991.
42. Henry CS, Zhong M, Lunte S.M., Kim M., Bau H, Santiago JJ: Ceramic microchips for capillary electrophoresis-electrochemistry; Anal Commun 36:1999.
43. Heyries KA, Tropini C, Vanlnsberghe M., Doolin C, Petriv OI, Singhal A, Leung K., Hughesman CB, Hansen CL: Megapixel digital PCR; Nat Methods 8:649; 2011.
44. Hill CR, Butler JM, Vallone PM: A 26plex autosomal STR assay to aid human identity testing; J Forensic Sci 54:1008; 2009.
45. Hopwood AJ, Hurth C, Yang J., Cai Z, Moran N, Lee-Edghill JG, Nordquist A., Lenigk R, Estes MD, Haley JP, et al.: Integrated microfluidic system for rapid forensic DNA analysis: Sample collection to DNA profile; Anal Chem 82:6991; 2010.
46. Horsman KM, Barker SLR, Ferrance JP, Forrest K.A., Koen KA, Landers JP: Separation of sperm and epithelial cells in a microfabricated device: Potential application to forensic analysis of sexual assault evidence; Anal Chem 77:742; 2005.
47. Hua ZS, Rouse JL, Eckhardt A.E., Srinivasan V., Pamula VK, Schell WA, Benton JL, Mitchell T.G., Pollack MG: Multiplexed real-time polymerase chain reaction on a digital microfluidic platform; Anal Chem 82:2310; 2010.
48. Hurth C, Smith SD, Nordquist A.R., Lenigk R., Duane B, Nguyen D, Surve A., Hopwood AJ, Estes MD, Yang J, et al.: An automated instrument for human STR identification: Design, characterization, and experimental validation; Electrophoresis 31:3510; 2010.
49. Innis MA, Myambo KB, Gelfand D.H., Brow MAD: DNA sequencing with thermus-aquaticus DNA-polymerase and direct sequencing of polymerase chain reaction-amplified DNA; Proc Natl Acad Sci USA 85:9436; 1988.
50. Iverson BD, Garimella SV: Recent advances in microscale pumping technologies: A review and evaluation; Micro-fluidics and Nano fluidics 5:145; 2008.
51. Jeffreys AJ, Wilson V, Thein SL: Hypervariable mini-satellite regions in human DNA; Nature 314:67; 1985.
52. Kailasa SK, Kang SH: Microchip-based capillary electrophoresis for DNA analysis in modern biotechnology: A review; Sep Purif Rev 38:242; 2009.
53. Karle M, Miwa J, Czilwik G., Auwarter V, Roth G, Zengerle R., von Stetten F: Continuous microfluidic DNA extraction using phase-transfer magnetophoresis; Lab on a Chip 10: 3284; 2010.
54. Karlinsey JM, Landers JP: AOTF-based multicolor fluorescence detection for short tandem repeat (STR) analysis in an electrophoretic microdevice; Lab on a Chip 8:1285; 2008.
55. Karlinsey JM, Landers JP: Multicolor fluorescence detection on an electrophoretic microdevice using an acoustooptic tunable filter; Anal Chem 78:5590; 2006.
56. Karlinsey JM, Monahan J, Marchiarullo D.J., Ferrance JP, Landers JP: Pressure injection on a valved microdevice for electrophoretic analysis of submicroliter samples; Anal Chem 77:3637; 2005.
57. Kim J, Johnson M, Hill P., Gale BK: Microfluidic sample preparation: Cell lysis and nucleic acid purification; Integr Biol 1:574; 2009.
58. Kiss MM, Ortoleva-Donnelly L, Beer N.R., Warner J., Bailey CG, Colston BW, Rothberg JM, Leamon J.H., Link DR: High-throughput quantitative polymerase chain reaction in picoliter droplets; Anal Chem 80:8975; 2008.
59. Koh CG, Tan W, Zhao M.Q., Fan ZH, Ricco AJ: Integrating polymerase chain reaction, valving, and electrophoresis in aplastic device forbacterial detection; Anal Chem 75:4591; 2003.
60. Lagally ET, Emrich CA, Mathies RA: Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis; Lab on a Chip 1:102; 2001.
61. Lang M: The Pocket Laboratory; Siemens'Pictures of the Future 74; Fall 2004.
62. Leslie DC, Easley CJ, Seker E, Karlinsey J.M., Utz M., Begley MR, Landers JP: Frequency-specific flow control in microfluidic circuits with passive elastomeric features; Nature Phys 5:231; 2009.
63. Liu JK, Lee ML: Permanent surface modification of polymeric capillary electrophoresis microchips for protein and peptide analysis; Electrophoresis 27:3533; 2006.
64. Liu P, Li X, Greenspoon S.A., Scherer JR, Mathies RA: Integrated DNA purification, PCR, sample cleanup, and capillary electrophoresis microchip for forensic human identification; Lab on a Chip 11:1041; 2011.
65. Liu P, Seo TS, Beyor N, Shin K.J., Scherer JR, Mathies RA: Integrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing; Anal Chem 79:1881; 2007.
66. Liu P, Yeung SHI, Crenshaw KA, Crouse C.A., Scherer JR, Mathies RA: Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer; Forensic Sci Int Genet 2:301; 2008.
67. Liu RH, Grodzinski P, Yang J., Lenigk R: Self-contained, fully integrated biochips for sample preparation, PCR amplification and DNA microarray analysis; In Liu RH, Lee AP (Eds): Integrated Biochips for DNA Analysis; Landes Bioscience: Austin, TX; p 46; 2007.
68. Liu Y, Fanguy JC, Bledsoe J.M., Henry CS: Dynamic coating using polyelectrolyte multilayers for chemical control of electroosmotic flow in capillary electrophoresis microchips; Anal Chem 72:5939; 2000.
69. Llopis SL, Osiri J, Soper SA: Surface modification of poly(methyl methacrylate) microfluidic devices for high-resolution separations of single-stranded DNA; Electrophoresis 28:984; 2007.
70. Lyon E, Wittwer CT: Light Cyclertechnology in molecular diagnostics; J Mol Diagn 11:93; 2009.
71. Manage DP, Morrissey YC, Stickel A.J., Lauzon J., Atrazhev A, Acker JP, Pilarski LM: On-chip PCR amplification of genomic and viral templates in unprocessed whole blood; Microfluid Nanofluid 10:697; 2011.
72. Manz A, Graber N, Widmer HM: Miniaturized total chemical analysis systems: A novel concept for chemical sensing; Sensor Actual B 1:244; 1990.
73. Markey AL, Mohr S, Day PJR: High-throughput droplet PCR; Methods 50:277; 2010.
74. McMahon G: Analytical Instrumentation: A Guide to Laboratory, Portable and Miniaturized Instruments; John Wiley and Sons: West Sussex, England; 2007.
75. MiDAS Rapid DNA Analysis; http://anbm.arizona.edu/research/current- projects-programs/rapid-dna-analysis (accessed December 2; 2011
76. Mitnick L, Carey L, Burger R., Desmarais S, Koutny L, Wernet O., Matsudaira P, Ehrlich D: High-speed analysis of multiplexed short tandem repeats with an electrophoretic microdevice; Electrophoresis 23:719; 2002.
77. Moss D, Harbison SA, Saul DJ: An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase; Int J Legal Med 117:340; 2003.
78. Nelson M: Making Sense of DNA Backlogs - Myths vs. Reality (National Institute of Justice Special Report); 2010.
79. Norris JV, Evander M, Horsman-Hall KM, Nilsson J., Laurell T, Landers JP: Acoustic differential extraction for forensic analysis of sexual assault evidence; Anal Chem 81:6089; 2009.
80. Norris JV, Root BE, Scott O.N., Marchiarullo D., Linke SJ, Reedy CR, Hagan KA, Pettit J, Mackness A., Bell R, et al.: Fully integrated, multiplexed STR-based human identification using a single microfluidic chip and automated instrument; Presentation-20th International Symposium on Human Identification; Las Vegas, NV; October 12-15, 2009.
81. O'Farrell PH: High resolution two-dimensional electrophoresis of proteins; J Biol Chem 250:4007; 1975.
82. Oh KW, Ahn CH: A review of microvalves; J Micromech Microeng 16:R13;2006.
83. Pekin D, Skhiri Y, Baret J.C., Le Corre D, Mazutis L, Ben Salem C, Millot F, El Harrak A, Hutchison JB, Larson J W, et al.: Quantitative and sensitive detection of rare mutations using droplet-based microfluidics; Lab on a Chip 11:2156; 2011.
84. Pjescic I, Tranter C, Hindmarsh P.L., Crews ND: Glass-composite prototyping for flow PCR with in situ DNA analysis; Biomed Microdevices 12:333; 2010.
85. Price CW, Leslie DC, Landers JP: Nucleic acid extraction techniques and application to the microchip; Lab on a Chip 9:2484; 2009.
86. Reedy CR, Bienvenue JM, Coletta L, Strachan B.C., Bhatri N., Greenspoon S, Landers JP: Volume reduction solid phase extraction of DNA from dilute, large-volume biological samples; Forensic Sci Int Genet 4:206; 2010.
87. Reedy CR, Hagan KA, Marchiarullo D.J., Dewald AH, Barron A, Bienvenue JM, Landers JP: A modular microfluidic system for deoxyribonucleic acid identification by short tandem repeat analysis; Anal Chim Acta 687:2011.
88. Reedy CR, Hagan KA, Strachan B.C., Higginson JJ, Bienvenue JM, Greenspoon SA, Ferrance J.P., Landers JP: Dual-domain microchip-based process for volume reduction solid phase extraction of nucleic acids from dilute, large volume biological samples; Anal Chem 82:5669; 2010.
89. Reedy CR, Price CW, Sniegowski J, Ferrance J.P., Begley M., Landers JP: Solid phase extraction of DNA from biological samples in a post-based, high surface area poly(methyl methacrylate) (PMMA) microdevice; Lab on a Chip 11:1603; 2011.
90. Root BE, Hagan KA, Reedy C.R., Norris JV, Egan M, Lovaglio R, Scott O.N., South DJ, Trost P, Albert D, et al.: A multisample platform for automated genetic analysis for human ID; Presertation - 22nd International Symposium on Human identification; National Harbor, MD; October 3-7, 2011.
91. Root BE, Reedy CR, Hagan K.A., Norris JV, Egan M, Lovaglio R, Scott O.N., South DJ, Trost P, Albert D, et al.: A multichannel microdevice for PCR amplification and electrophoretic separation of DNA; Presentation - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Seattle, WA; October 2-6, 2011.
92. Saiki RK, Scharf S, Faloona F., Mullis KB, Horn GT, Erlich HA, Arnheim N: Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle-cell ainemia; Science 230:1350; 1985.
93. Schaerli Y, Wootton RC, Robinson T, Stein V., Dunsby C, Neil MAA, French PMW, deMello AJ, Abell C, Hollfelder F: Continuous-flow polymerase chain reaction of single-copy DNA in micro fluidic microdroplets; Anal Chem 81: 302; 2009.
94. Schmalzing D, Koutny L, Adoourian A., Belgrader P, Matsudaira P. Ehrlich E: DNA typing in thirty seconds with a microfabricated device; Proc Natl Acad Sci USA 94:10273; 1997.
95. Schmalzing D, Koutny L, Chisholm D., Adoourian A, Matsudaira P, Ehrlich D: Two-color multiplexed analysis of eight short tandem repeat loci with an electrophoretic microdevice; Analy Bio chem 270:148; 1999.
96. Scientific Working Group on DNA Analysis Methods (SWGDAM): SWGDAM interpretation guidelines for autosomal STR typing by forensic DNA testing laboratories; http://www.fbi.gov/about-us/lab/codis/swgdam-interpretation- guidelines (accessed March 5, 2012).
97. Shaw KJ, Docker PT, Yelland J.V., Dyer CE, Greenman J, Greenway GM, Haswell SJ: Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling; Lab on a Chip 10:1725; 2010.
98. Shen F, Du WB, Kreutz J.E., Fok A., Ismagilov RF: Digital PCR on a SlipChip; Lab on a Chip 10:2666; 2010.
99. Shi Y, Anderson RC: High-resolution single-stranded DNA analysis on 4.5 cm plastic electrophoretic micro-channels; Electrophoresis 24:3371; 2003.
100. Sijen T, Benschop CCG, Wiebosch DC, Kloosterman AD: Post-coital vaginal sampling with nylon flocked swabs improves DNA typing; Forensic Sci Int Genet 4: 115; 2010.
101. Sikanen T, Heikkila L, Tuornikoski S., Ketola RA, Kostiainen R, Franssila S, Kotiaho T: Performance of SU-8 microchips as separation devices and comparison with glass microchips; Anal Chem 79:6255; 2007.
102. Sinville R, Sdper SA: High resolution DNA separations using microchip electrophoresis; J Sep Sci 30:1714; 2007.
103. Steadman SA; McDonald JD, Andrews JS, Watson ND: Recovery ana STR amplification of DNA from RFLP membranes; J Forensic Sci 53:349; 2008.
104. Sun Y, Kwok YC, Nguyen NT: A circular ferrofluid driven microchip for rapid polymerase chain reaction; Lab on a Chip 7:1012; 2007.
105. Sun Y, Lee PFP, Nguyen NT, Kwok YC: Rapid amplifi cation of genetically modified organisms using a circular ferrofluid-driven PCR microchip; Anal Bioanal Chem 394:1505; 2009.
106. Tan E, Giese H, Hartmann DM: Microfluidic DNA Extraction and Purification from Forensic Samples: Towards Rapid, Fully Integrated STR Analysis (Final report to the National Institute of Justice); https://www.ncjrs.gov/pdffilesl/ nij/grants/226810.pdf (accessed March 5, 2012).
107. Tian HJ, Huhmer AFR, Landers JP: Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format; Anal Biochem 283:175; 2000.
108. Ugaz VM, Brahmasandra SN, Burke D.T., Burns MA: Cross-linked polyacrylamine gel electrophoresis of single-stranded DNA for microfabricated genomic analysis systems; Electrophoresis 23:1450; 2002.
109. Unger MA, Chou H, Thorsen T., Scherer A, Quake SR: Monolithic microfabricated valves and pumps by multilayer soft lithography; Science 288:113; 2000.
110. Vallone PM, Hill CR, Butler JM: Demonstration of rapid multiplex PCR amplification involving 16 genetic loci; Forensic Sci Int Genet 3:42; 2008.
111. Vogelstein B, Kinzier KW: Digital PCR; PNAS 96:9236; 1999.
112. Wang J, Pumera M. Chatrathi MP, Escarpa A, Konrad R, Griebel A, Dorner W, Lowe H: Towards disposable lab-on-a-chip: Poly(methylmethacrylate) microchip electrophoresis device with electrochemical detection; Electrophoresis 23:596; 2002.
113. Wen J, Legendre LA, Bienvenue J.M., Landers JP: Purification of nucleic acids in microfluidic devices; Anal Chem 80:6472; 2008.
114. Witek MA, Llopis SD, Wheatley A, McCarley R.L., Soper SA: Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips; Nucleic Acids Res 34:e74; 2006.
115. Wittwer CT, Ririe KM, Andrew R.V., David DA, Gundry RA, Balis UJ: The Light Cycler(TM) a microvolume multisample fluorimeter with rapid temperature control; Bio Techniques 22:176; 1997.
116. Wu W, Lee NY: Three-dimensional on-chip continuous-flow polymerase chain reaction employing a single heater; Anal Bioanal Chem 400:2053; 2011.
117. Yeung SHI, Greenspoon SA, McGuckian A, Crouse C.A., Emrich CA, Ban J, Mathies RA: Rapid and high-throughput forensic short tandem repeat typing using a 96-lane microfabricated capillary array electrophoresis microdevice; J Forensic Sci 51:740; 2006.
118. Zhang CS, Xing D: Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends; Nucleic Acids Res 35:4223; 2007.
119. Zhang YH, Ozdemir P: Microfluidic DNA amplification - A review; Analy Chim Acta 638:115; 2009.