The LightCycler(TM): A microvolume multisample fluorimeter with rapid temperature control

BioTechniques

Volumn 22, Issue 1, 1997, Pages 176-181

  Wittwer, C T ^a,b   Ririe, K M ^a   Andrew, R V ^a   David, D A ^a   Gundry, R A ^a   Balis, U J ^a  

^a Idaho Technology Inc   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; FLUORESCEIN; RHODAMINE; CARBOCYANINE; CYANINE DYE 5; DNA; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; ORGANIC COMPOUND; SYBR GREEN I;

ARTICLE; DNA PROBE; DNA SEQUENCE; FLUORESCENCE; FLUOROMETRY; GENE AMPLIFICATION; OPTICS; TEMPERATURE DEPENDENCE; CALORIMETRY; CELL SEPARATION; FLOW CYTOMETRY; INSTRUMENTATION;

CALORIMETRY; CARBOCYANINES; CELL SEPARATION; DNA; FLOW CYTOMETRY; FLUORESCEIN; FLUORESCEINS; FLUORESCENT DYES; FLUOROMETRY; ORGANIC CHEMICALS; RHODAMINES;

EID: 0030614426     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97221pf02     Document Type: Article

References (30)

1. Black, S.F., D.I. Gray, D.R. Fenlon and R.G. Kroll. 1995. Rapid RAPD analysis for distinguishing listeria species and listeria-monocytogenes serotypes using a capillary air thermal cycler. Lett. Appl. Microbiol. 20:188-190.
2. Buck, G.E. 1996. Detection of Bordetella pertussis by rapid-cycle PCR and colorimetric microwell hybridization. J. Clin. Microbiol. 34:1355-1358.
3. Burns, M.A., C.H. Mastrangelo, T.S. Sammarco, F.P. Man, J.R. Webster, B.N. Johnson, B Foerster, D. Jones, Y. Fields, A.R. Kaiser and D.T. Burke. 1996. Microfabricated structures for integrated DNA analysis. Proc. Natl. Acad. Sci. USA 93:5556-5561.
4. Cheng, J., M.A. Shoffner, K.R. Mitchelson, L.J. Kricka and P. Wilding. 1996. Analysis of ligase chain reaction products amplified in a silicon-glass chip using capillary electropheresis. J. Chromatogr. A 732:151-158.
5. Cheng, J., M.A. Shoffner, G.E. Hvichia, L.J. Kricka and P. Wilding. 1996. Chip PCR. II. Investigations of different PCR amplification systems in microfabricated silicon-glass chips. Nucleic Acids Res. 24:380-385.
6. Ghosh, S.S., P.S. Eis, K. Blumeyer, K. Fearon and D.P. Millar. 1994. Real time kinetics of restriction endonuclease cleavage monitored by fluorecence resonance energy transfer. Nucleic Acids Res. 22:3155-3159.
7. Higuchi, R., G. Dollinger, P.S. Walsh and R. Griffith. 1992. Simultaneous amplification and detection of specific DNA sequences. Bio/Technology 10:413-417.
8. Higuchi, R., C. Fockler, G. Dollinger and R. Watson. 1993. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Bio/Technology 11:1026-1030.
9. Houston, P. and T. Kodadek. 1994. Spectrophotometric assay for enzyme-mediated unwinding of double-stranded DNA. Proc. Natl. Acad. Sci. USA 91:5471-5474.
10. Ishiguro, T., J. Saitch, H. Yawata, H. Yamagishi, S. Iwasaki and Y. Mitoma. 1995. Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater. Anal. Biochem. 229:207-213.
11. Lee, L.G., C.R. Connell and W. Bloch. 1993. Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Res. 21:3761-3766.
12. Matayoshi, E.D., G.T. Wang, G.A. Krafft and J. Erickson. 1990. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science 247:954-958.
13. Morrison, L.E. and L.M. Stols. 1993. Sensitive fluorescence-based thermodynamic and kinetic measurements of DNA hybridization in solution. Biochemistry 32:3095-3104.
14. Mouritsen C.L., C.T. Wittwer, C.M. Litwin, L. Yang, J.J. Weis, T.B. Martins, T.D. Jaskowski and H.H. Hill. 1996. Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses. Am. J. Clin. Pathol. 105:647-654.
15. Patel, L.R., T. Curran and T.K. Kerppola. 1994. Energy transfer analysis of Fos-Jun dimerization and DNA binding. Proc. Natl. Acad. Sci. USA 91:7360-7364.
16. Ririe, K.M., R.P. Rasmussen and C.T. Wittwer. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. (In press).
17. Rye, H.S., J.M. Dabora, M.A. Quesada, R.A. Mathies and A.N. Glazer. 1993. Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal. Biochem. 208:144-150.
18. Shapiro, H.M. 1995. Practical Flow Cytometry, 3rd ed. Wiley-Liss, New York.
19. Stokke, T., C. Colins, W.L. Kuo, D. Kowbel, F. Shadravan, M. Tanner, A. Kallioniemi, O.P. Kallioniemi, D. Pinkel, L. Deaven and J.W. Gray. 1995. A physical map of chromosome 20 established using fluorescence in situ hybridization and digital image analysis. Genomics 26:134-137.
20. Tuschl, T., C. Gohlke, T.M. Jovin, E. Westhof and F. Eckstein. 1994. A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements. Science 266:785-789.
21. Walker, G.T., C.P. Linn and J.G. Nadeau. 1996. DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein. Nucleic Acids Res. 24:348-353.
22. Wittwer, C.T., G.C. Fillmore and D.J. Garling. 1990. Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal. Biochem. 186:328-331.
23. Wittwer, C.T., G.C. Fillmore and D.R. Hillyard. 1989. Automated polymerase chain reaction in capillary tubes with hot air. Nucleic Acids. Res. 17:4353-4357.
24. Wittwer, C.T. and D.J. Garling. 1991. Rapid cycle DNA amplification: time and temperature optimization. BioTechniques 10:76-83.
25. Wittwer, C.T., B.C. Marshall, G.B. Reed and J.L. Cherry. 1993. Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus. Clin. Chem. 39:804-809.
26. Wittwer, C.T., M.G. Herrmann, A.A. Moss and R.P. Rasmussen. 1997. Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques 22:130-138.
27. Wittwer, C.T., G.B. Reed and K.M. Ririe. 1994. Rapid cycle DNA amplification, p. 174-181. In K.B. Mullis, F. Ferre and R.A. Gibbs (Eds.), The Polymerase Chain Reaction. Birkhauser, Boston.
28. Wittwer, C.T., K.M. Ririe and R.P. Rasmussen. Fluorescence monitoring of rapid cycle PCR for quantification, In F. Ferre (ed.), Gene Quantification. Birkhauser, Boston (In press).
29. Wu, P. and L. Brand. 1994. Resonance energy transfer: methods and applications. Anal. Biochem. 218:1-13.
30. Yguerabide, J. and A. Ceballos. 1995. Quantitative fluorescence method for continuous measurement of DNA hybridization kinetics using a fluorescent intercalator. Anal. Biochem. 228:208-220.