Application of FTA® technology to extraction of sperm DNA from mixed body fluids containing semen

Legal Medicine

Volumn 8, Issue 1, 2006, Pages 43-47

  Fujita, Yoshihiko ^a,b   Kubo, Shin Ichi ^a  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b Scientific Investigation Research Laboratory   (Japan)

Author keywords

Body fluid mixed with semen; Forensic casework; FTA technology; Sexual assault; Short tandem repeat (STR) analysis; Sperm origin DNA extraction

Indexed keywords

DITHIOTHREITOL; PROTEINASE K; DNA; WATER;

ADULT; ARTICLE; BODY FLUID; BODY WATER; CARD; CELL FRACTIONATION; CONTROLLED STUDY; DEVICE; DNA EXTRACTION; DNA PURIFICATION; ENZYME DEGRADATION; EPITHELIUM CELL; GENETIC PROCEDURES; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; POLYMERASE CHAIN REACTION; SALIVA; SEPARATION TECHNIQUE; SPERM; SUPERNATANT; SUSPENSION; TECHNOLOGY; DNA FINGERPRINTING; FEMALE; GENOTYPE; INSTRUMENTATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; RAPE; SPERMATOZOON; TANDEM REPEAT; VAGINA;

DNA; DNA FINGERPRINTING; ENDOPEPTIDASE K; FEMALE; GENOTYPE; HUMANS; MALE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; RAPE; SALIVA; SPERMATOZOA; TANDEM REPEAT SEQUENCES; VAGINA; WATER;

EID: 29144491428     PISSN: 13446223     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.legalmed.2005.06.007     Document Type: Article

References (9)

1. P. Gill, A.J. Jefferys, and D.J. Werrett Forensic application of DNA 'fingerprints' Nature 318 1985 577 579
2. A. Giusti, M. Baird, S. Pasquale, I. Balazs, and J. Glassberg Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm J Forensic Sci 31 1986 409 417
3. M. Iwasaki, S. Kubo, M. Ogata, and I. Nakasono A demonstration of spermatozoa on vaginal swabs complete destraction of vaginal cell deposits J Forensic Sci 34 1989 659 664
4. K. Yoshida, K. Sekiguchi, N. Mizuno, K. Kasai, I. Sakai, H. Sato, and S. Seta The modified method of two-step differential extraction of sperm and vaginal epithelial cell DNA from vaginal fluid mixed with semen Forensic Sci Int 72 1995 25 33
5. ® paper and the Fosys Plato robotic microplate processor. Proceeding from the first European symposium on human identification 1996 Promega Corporation p. 20
6. M.J. La Fountain, M.B. Schwartz, P.A. Svete, M.A. Walkinshaw, and E. Buel TWGDAM validation of the AmpFlSTR profiler plus and AmpFlSTR cofilter STR multiplex system using capillary electrophoresis J Forensic Sci 46 2001 1191 1198
7. M.S. Nelson, E.N. Levedakou, J.R. Matthews, B.E. Early, D.A. Freeman, C.A. Kuhn, C.J. Sprecher, A.S. Amin, K.C. McElfresh, and J.W. Schumm Detection of primer-binding site polymorphism for the STR locus D16S539 using the Powerplex™ 1.1 sstm and validation of a degenerate primer to correct for the polymorphism J Forensic Sci 47 2002 345 349
8. B.E. Krenke, A. Tereba, S.J. Anderson, E. Buel, S. Culhane, C.J. Finis, C.S. Tomsey, J.M. Zachetti, and C.J. Sprecher Validaion of a 16-locus fluorescent multiplex system J Forensic Sci 47 2002 773 785
9. L.H. Seah, N.H. Jeevan, M.I. Othman, P. Jaya, Y.S. Ooi, P.C. Wong, and S.S. Kee STR data for the AmpFlSTR identifiler loci in three ethnic groups (Malay, Chinese, Indean) of the Malaysian population Forensic Sci Int 138 2003 134 137