Microchip-based capillary electrophoresis for DNA analysis in modern biotechnology: A review

Separation and Purification Reviews

Volumn 38, Issue 3, 2009, Pages 242-288

  Kailasa, Suresh Kumar ^a,b,c   Kang, Seong Ho ^a  

^a Chonbuk National University   (South Korea)

^b NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

^c NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

Author keywords

DNA; Genetically modified organisms; Microchip electrophoresis; Programmed field strength gradients

Indexed keywords

ANALYTICAL TECHNOLOGY; CLINICAL RESEARCH; DNA ANALYSIS; DNA FRAGMENT; FIELD STRENGTHS; GENETICALLY MODIFIED ORGANISMS; MICRO-FLUIDIC DEVICES; MICROCHIP ELECTROPHORESIS; MODERN BIOLOGY; MOLECULAR MANIPULATION; POTENTIAL APPLICATIONS; PROGRAMMED FIELD STRENGTH GRADIENTS; SIMULTANEOUS ANALYSIS; SMART DEVICES; VOLUME ANALYSIS;

BIOLOGY; BIOTECHNOLOGY; CAPILLARY ELECTROPHORESIS; COMPOSITE MICROMECHANICS; DNA; ELECTROCHEMISTRY; MICROPROCESSOR CHIPS; NUCLEIC ACIDS;

GENES;

EID: 70449635653     PISSN: 15422119     EISSN: 15422127     Source Type: Journal    
DOI: 10.1080/15422110903095136     Document Type: Article

References (157)

1. Dolnik, V. and Liu, S. (2005) Application of capillary electrophoresis on microchip. J. Sep. Sci., 28: 1994-2009
2. Dolnik, V., Liu, S., and Jovanovich, S. (2000) Capillary electrophoresis on microchip. Electrophoresis, 21: 41-54
3. Li, S.F.Y. and Kricka, J.L. (2006) Clinical analysis by microchip capillary electrophoresis. Clin. Chem., 52: 37-45
4. Hjerten, S. (1967) Free zone electrophoresis. Chromatogr. Rev., 9: 122-219
5. Jorgenson, J.W. and Lukacs, K.D. (1981) Zone electrophoresis in opentubular glass capillaries. Anal. Chem., 53: 1298-1302
6. Jacobson, S.C., Hergenroder, R., Koutny, L.B., and Ramsey, J. (1994) Open channel electrochromatography on a microchip. Anal. Chem., 66: 2369-2373
7. Manz, A., Graber, N., and Widmer, H.M. (1990) Miniaturized total chemical analysis systems: A novel concept for chemical sensing. Sensors and Actuators B, 1: 244-248
8. Reyes, D.R., Lossifidis, D., Auroux, P.A., and Manz, A. (2002) Micro total analysis systems. 1. Introduction, theory and technology. Anal. Chem., 74: 2623-2636 (Pubitemid 34651165)
9. Verpoorte, E., Manz, A., Lüdi, H., Bruno, A.E., Maystre, F., Krattiger, B., Widmer, H.M., van der Schoot, B.H., de Rooij, N.F. (1992) A silicon flow cell for optical detection in miniaturized total chemical analysis systems. Sensors and Actuators B, 6: 66-70
10. Vandaveer IV, W.R., Pasas-Farmer, S.A., Fischer, D.J., Frankenfeld, C.N., and Lunte, S. M. (2004) Recent developments in electrochemical detection for microchip capillary electrophoresis. Electrophoresis, 25: 3528-3549
11. Khandurina, J. and Guttman, A. (2002) Bioanalysis in microfluidic devices. J. Chromatogr. A, 943: 159-183
12. Pumera, M. (2005) Microchip-based electrochromatography: designs and applications. Talanta, 66: 1048-1062
13. Feng, X. and Baba, Y. (2004) Polymer solutions and entropic-based systems for double-stranded DNA capillary electrophoresis and microchip electrophoresis. Electrophoresis, 25: 2332-2345
14. Lee, S.J. and Lee, S.Y. (2004) Micro total analysis system (m-TAS) in biotechnology. Appl. Microbiol. Biotechnol., 64: 289-299
15. Hataoka, Y., Zhang, L., Yukimasa, T., and Baba, Y. (2005) Rapid microvolume PCR of DNA confirmed by microchip electrophoresis. Anal. Sci., 21: 53-56
16. Liu, Y., Foote, R.S., Culbertson, C.T., Jacobson, S.C., Ramsey, R.S., and Ramsey, J.M. (2000) Electrophoretic separation of proteins on microchips. J. Microcolumn Separ., 12: 407-411
17. Chen, Y. and Chen, S. (2000) Analysis of DNA fragments by microchip electrophoresis fabricated on poly(methyl methacrylate) substrates using a wire-imprinting method. Electrophoresis, 21: 165-170
18. Baldessari, F. and Santiago, J.G. (2006) Electrophoresis in nanochannels: brief review and speculation. J. Nanobiotechnol., 4: 1-6
19. Liu, S., Ren, H., Gao, Q., Roach, D.J., Loder, R.T., Armstrong, T.M., Mao, Q., Blaga, I., Barker, D.L., and Jovanovich, S.B. (2000) Automated parallel DNA sequencing on multiple channel microchips. PNAS, 10: 5369-5374
20. Tian, H.J., Brody, L.C., and Landers, J.P. (2000) Rapid detection of deletion, insertion, and substitution mutations via heteroduplex analysis using capillary- and microchip-based electrophoresis. Genome Res., 10: 1403-1413
21. Zhang, L., Dang, F., and Baba, Y. (2003) Microchip electrophoresis-based separation of DNA. J. Pharmaceut. Biomed. Anal., 30: 1645-1654
22. Harrison, D.J., Manz, A., Fan, Z., Luedi, H., and Widmer, H.M. (1992) Capillary electrophoresis and sample injection systems integrated on a planar glass chip. Anal. Chem., 64: 1926-1932
23. Mathies, R.A., Emrich, C.A., Tian, H., Medintz, I., Berti, L., and Tom, J. (2001) in: Ramsey, J.M. and van der Berg A. (Eds.), Micro Total Analysis Systems, Kluwer Academic Publishers, Dordrecht, pp. 13-15
24. Gao, Q., Shi, Y., and Liu, S. (2001) Multiple-channel microchips for highthroughput DNA analysis by capillary electrophoresis. Fres. J. Anal. Chem., 371: 137-145
25. Medintz, I.L., Paegel, B.M., Blazej, R.G., Emrich, C.A., Berti, L., Scherer, J.R., and Mathies, R.A. (2001) High-performance genetic analysis using microfabricated capillary array electrophoresis microplates. Electrophoresis, 22: 3845-3856
26. Huang, Z., Munro, N., Hühmer, A.F.R., and Landers, J.P. (1999) Acoustooptical deflection-based laser beam scanning for fluorescence detection on multichannel electrophoretic microchips. Anal. Chem., 71: 5309-5314
27. Simpson, J.W., Ruiz-Martinez, M.C., Mulhern, G.T., Berka, J., Latimer, D.R., Ball, J.A., Rothberg, J.M., and Went, G.T. (2000) A transmission imaging spectrograph and microfabricated channel system for DNA analysis. Electrophoresis, 21: 135-149
28. Woolley, A.T., Sensabaugh, G.F., and Mathies, R.A. (1997) High-speed DNA genotyping using microfabricated capillary array electrophoresis chips. Anal. Chem., 69: 2181-2186
29. Simpson, P.C., Roach, D., Woolley, A.T., Thorsen, T., Johnston, R., Sensabaugh, G.F., and Mathies, R.A. (1998) High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates. Proc. Natl. Acad. Sci. USA, 95: 2256-2261
30. Paegel, B.M., Emrich, C.A., Wedemayer, G.J., Scherer, J.R., and Mathies, R.A. (2002) High throughput DNA sequencing with a microfabricated 96- lane capillary array electrophoresis bioprocessor. Proc. Natl. Acad. Sci. USA, 99: 574-579
31. Liu, D., Zhou, X., Zhong, R., Ye, N., Chang, G., Xiong, W., Mei, X., and Lin, B. (2006) Analysis of multiplex PCR fragments with PMMA microchip electrophoresis. Talanta, 68: 616-622
32. Lee, J.C. and Gutell, R.R. (2004) Diversity of base-pair conformations and their occurrence in rRNA structure and RNA structural motifs. J. Mol. Biol., 344: 1225-1249
33. Barciszewski, J. and Frederic, B.F.C. (eds.). (1999) RNA Biochemistry and Biotechnology. Kluwer Academic: Boston, MA, pp. 73-87
34. Alberts, B., Alexander J., Lewis, J., Raff, M., Roberts, K., and Walters, P. (2002) Molecular Biology of the Cell, Fourth Edition. Garland Science: New York and London
35. Pauling, L. (1960) The Nature of the Chemical Bond, 3rd. ed., Cornell University Press: New York
36. Weiss, B., Davidkova, G., and Zhou, L.W. (1999) Antisense RNA gene therapy for studying and modulating biological processes. Cell. Mol. Life Sci., 55: 334-358
37. Quesada, M.A. and Menchen, S. (2001) Replaceable polymers for DNA sequencing by capillary electrophoresis. Methods Mol. Biol., 162: 139-166
38. Cohen, A.S. and Karger, B.L. (1987) High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins. J. Chromatogr., 397: 409-417
39. Gao, Q. and Yeung, E.S. (1998) A matrix for DNA separation: genotyping and sequencing using poly(vinylpyrrolidone) solution in uncoated capillaries. Anal. Chem., 70: 1382-1388 (Pubitemid 128669453)
40. Song, J.M. and Yeung, E.S. (2001) Optimization of DNA electrophoretic behavior in poly(vinyl pyrrolidone) sieving matrix for DNA sequencing. Electrophoresis, 22: 748-754
41. Kailasa, S.K. and Kang, S.H. (2007) Ultra-fast simultaneous analysis of GMOs in maize by microchip electrophoresis with laser-induced fluorescence detector. Electrophoresis, 28: 4247-4254
42. Rodriguez, I., Jin, L.J., and Li, S.F.Y. (2000) High-speed chiral separations on microchip electrophoresis devices. Electrophoresis, 21: 211-219
43. Tian, H., Jaquins-Gerstl, A., Munro, N., Trucco, M., Brody, L.C., and Landers, J.P. (2000) Single strand conformation polymorphism analysis by capillary and microchip electrophoresis: A fast, simple method for detection of common mutations in BRCA1 and BRCA2. Genomics, 63: 25-34
44. Ronal, Z., Barta, C., Sasvari-Szekely, M., and Guttman, A. (2001) DNA analysis on electrophoretic microchips: Effect of operational variables. Electrophoresis, 22: 294-299
45. Huang, X. and Ren, J. (2006) Chemiluminescence detection for capillary electrophoresis and microchip capillary electrophoresis, Trends Anal. Chem., 25: 155-166 (Pubitemid 43152037)
46. Vandaveer IV, W.R., Pasas, S.A., Martin, R.S., and Lunte, M.S. (2002) Recent developments in amperometric detection for microchip capillary electrophoresis. Electrophoresis, 23: 3667-3677
47. Turner, G.K. (1985) Measurement of light from chemical or biochemical reactions, in bioluminescence and chemiluminescence: Instruments and Applications, Vol.I,K. Van Dyke, Ed., CRC Press: Boca Raton, FL, pp. 43-78
48. Aldo, R., Mara, M., Simona, V., Monica, C., Francesca, B., Mario, B., Patrizia, P., Marialuisa, Z., and Monica, M. (2002) Microliter format for simultaneous multianalyte detection and development of a PCR chemiluminescent enzyme immunoassay for typing human papillomavirus DNAs. Clin. Chem., 48: 1654-1660
49. Wu, X., Zhou, Y., and Xu, S. (2004) Chemiluminescent detection of genetic polymorphisms based on mismatch hybridization: Application to cytochrome P4501A1. Mol. Cell. Probes, 18: 17-22
50. Zhao, X., You, T., Qiu, H., Yan, J., Yang, X., and Wang, E. (2004) Electrochemiluminescence detection with integrated indium tin oxideelectrode on electrophoretic microchip for direct bioanalysis of lincomycin in the urine. J. Chromatogr. B, 810: 137-142
51. Ansorge, W., Sproat, B.S., Stegemann, J., and Schwager, C. (1986) A nonradioactive automated method for DNA sequence determination. J. Biochem. Biophys. Meth., 13: 315-323
52. Ansorge, W., Sproat, B., Stegemann, J., Schwager, C., and Zenke, M. (1987) Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucl. Acids Res., 15: 4593-4602
53. Prober, J.M., Trainor, G.L., Dam, R.J., Hobbs, F.W., Robertson, C.W., Zagursky, R.J., Cocuzza, A.J., Jensen, M.A., and Baumeister, K. (1987) A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. Science, 238: 336-341
54. Smith, L.M., Sanders, J.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. (1986) Fluorescence detection in automated DNA sequence analysis. Nature, 321: 674-679
55. Jang, S., Cho, K., Chae, J.S., and Kang, S.H. (2004) Fast diagnosis of bovine theileriosis by whole blood PCR and microchip electrophoresis. Bull. Korean Chem. Soc., 25: 757-760
56. Kang, S.H., Jang, S., and Park, S.K. (2006) Single-strand conformation polymorphism analysis by microchip electrophoresis for the rapid detection of point mutation in human obesity gene. Bull. Korean Chem. Soc., 27: 1346-1352
57. Kang, S.H., Park, M., and Cho, K. (2005) Separation of DNA fragments for fast diagnosis by microchip electrophoresis using programmed field strength gradient. Electrophoresis, 26: 3179-3184
58. Kim, Y.J., Chae, J.S., Chang, J.K., and Kang, S.H. (2005) Microchip capillary gel electrophoresis using programmed field strength gradients for the ultra-fast analysis of genetically modified organisms in soybeans. J. Chromatogr, A, 1083: 179-184
59. Gong, M., Wehmeyer, K.R., Limbach, P.A., and Heineman, W.R. (2007) Frontal analysis in microchip CE: A simple and accurate method for determination of protein-DNA dissociation constant. Electrophoresis, 28: 837-842
60. Ivan, R., Sergio, C., Alessandro, R., Massimo, G., Marcella, C., and Marina, C. (2006) Microchip and single-photon avalache diodes for DNA separation with high sensitivity. Electrophoresis, 27: 3797-3804
61. Tian, H.J. and Landers, J.P. (2002) Hydroxyethylcellulose as an effective polymer network for DNA analysis in uncoated glass microchips: optimization and application to mutation detection via heteroduplex analysis. Anal. Biochem., 309: 212-223
62. Wang, J. and Pumera, M. (2002) Dual conductivity/amperometric detection system for microchip capillary electrophoresis. Anal. Chem., 74: 5919-5923
63. Tanyanyiwa, J., Leuthardt, S., and Hauser, P.C. (2002) Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis. Electrophoresis, 23: 3659-3666 (Pubitemid 35469547)
64. Lacher, N.A. and Lunte, S.M. (2004) Development of a microfabricated palladium decoupler/electrochemical detector for microchip capillaryelectrophoresis using a hybrid glass/poly(dimethylsiloxane) device. Anal. Chem., 76: 2482-2491
65. Castano-Alvarez, M., Fernandez-Abedul, M., and Costa-Garcia, A. (2006) Analytical performance of CE microchips with amperometric detection instrumentation. Science & Technology, 34, pp. 697-710
66. Gavin, P.F. and Ewing, A.G. (1996) Continuous separations with microfabricated electrophoresis-electrochemical array detection. J. Am. Chem. Soc., 118: 8932-8936
67. Gavin, P.F. and Ewing, A.G. (1997) Characterization of electrochemical array detection for continuous channel electrophoretic separations in micrometer and submicrometer channels. Anal. Chem., 69: 3838-3845
68. Gavin, P.F. and Ewing, A.G. (1998) Characterization of lateral dispersion in microfabricated electrophoresis-electrochemical array detection. J. Microcolumn Separ., 10: 357-364
69. Woolley, A.T., Lao, K., Glazer, A.N., and Mathies, R.A. (1998) Capillary electrophoresis chips with integrated electrochemical detection. Anal. Chem., 70: 684-688
70. Shiddiky, M.J.A., Kim, R.E., and Shim, Y.B. (2005) Microchip electrophoresis with a cellulose-DNA modified screen printed electrode for the analysis of neurotransmitters. Electrophoresis, 26: 3043-3052
71. Vlckova, M. and Schwarz, M.A. (2007) Determination of cationic neurotransmitters and metabolites in brain homogenates by microchip electrophoresis and carbon nanotube-modified amperometry. J. Chromatogr. A, 1142: 214-221
72. Shiddiky, M.J.A. and Shim, Y.B. (2007) Trace analysis of DNA: preconcentration, separation, and electrochemical detection in microchip electrophoresis using Au nanoparticles. Anal. Chem., 79: 3724-3733
73. Kim, J.H.,Kang, C.J., and Kim, S.Y. (2005) Development of a microfabricated disposable microchip with a capillary electrophoresis and integrated threeelectrode electrochemcial detection. Biosensors Bioelectron., 20: 2314-2317
74. Lin, Y.W., Huang, M.J., and Chang, H.T. (2003) Analysis of doublestranded DNA by microchip capillary electrophoresis using polymer solutions containing gold nanoparticles. J. Chromatogr. A, 1014: 47-55
75. Pan, D. and Mathies, R.A. (2001) Chromophore structure in lumirhodopsin and metarhodopsin I by time resolved resonance Raman microchip spectroscopy. Biochemistry, 40: 7929-7936
76. Tamaki, E., Sato, K., Tokeshi, M., Sato, K., Aihara, M., and Kitamori, T. (2002) Single-cell analysis by a scanning thermal lens microscope with a microchip: Direct monitoring of cytochrome c distribution during apoptosis process. Anal. Chem., 74: 1560-1564
77. Munson, M.S., Hasenbank, M.S., Fu, E., and Yager, P. (2004) Suppression of nonspecific adsorption using sheath flow. Lab on a Chip, 4: 438-445
78. Costin, C.D. and Synovec, R.E. (2002) A microscale-molecular weight sensor: Probing molecular diffusion between adjacent laminar flows by refractive index gradient detection. Anal. Chem., 74: 4558-4565
79. Burggraf, N., Krattiger, B., de Mello, A.J., de Rooij, N.F., and Manz, A. (1998) Holographic refractive index detector for application in microchipbased separation systems. Analyst, 123: 1443-1447
80. Xue, Q., Dunayevskiy, Y.M., Foret, F., and Karger, B.L. (1997) Integrated multichannel microchip electrospray ionization mass spectrometry: Analysis of peptides from on-chip tryptic digestion of melittin. Rapid Commun. Mass Spectrom., 11: 1253-1256
81. Ramsey, R.S. and Ramsey, J.M. (1997) Generating electrospray from microchip devices using electroosmotic pumping. Anal. Chem., 69: 1174-1178
82. Lazar, I.M., Ramsey, R.S., Sundberg, S., and Ramsey, J.M. (1999) Subattomole-sensitivity microchip nanoelectrospray source with time-offlight mass spectrometry detection. Anal. Chem., 71: 3627-3631
83. Limbach, P.A. and Meng, Z. (2002) Integrating micromachined devices with modern mass spectrometry. Analyst, 127: 693-700
84. Foret, F. and Preisler, J. (2002) Liquid phase interfacing and miniaturization in matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 2: 360-372
85. Figeys, D. (2002) Adapting arrays and lab-on-a-chip technology for proteomics, Proteomics. 2: 373-382
86. Ohnesorge, J., Neusüß, C., and Wätzig, H. (2005) Quantitation in capillary electrophoresis-mass spectrometry. Electrophoresis, 26: 3973-3987
87. Lazar, I.M., Grym, J., and Foret, F. (2006) Microfabricated devices: A new sample introduction approach to mass spectrometry. Mass Spectrom. Rev., 25: 573-594
88. DeVoe, D.L. and Lee, C.S. (2006) Microfluidic technologies for MALDIMS in proteomics. Electrophoresis. 27: 3559-3568
89. Wang, Y.X., Cooper, J.W, Lee, C.S., and DeVoe, D.L. (2004) Efficient electrospray ionization from polymer microchannels using integrated hydrophobic membranes. Lab Chip, 4: 363-367
90. Brivio, M., Oosterbroeck, R.E., Verboom, W., van den Berg, A., and Reinhoudt, D.N. (2005) Simple chip-based interfaces for on-line monitoring of supramolecular interactions by nano-ESI MS. Lab Chip, 5: 1111-1122
91. Li, J., Kelly, J.F., Chernushevich, I., Harrison, D.J., and Thibault, P. (2000) Separation and identification of peptides from gel-isolated membrane proteins using a microfabricated device for combined capillary electrophoresis/ nanoelectrospray mass spectrometry. Anal. Chem., 72: 599-609
92. Li, J., Thibault, P., Bings, N.H., Skinner, C.D., Wang, C., Colyer, C., and Harrison, J. (1999) Integration of microfabricated devices to capillary electrophoresis-electrospray mass spectrometry using a low dead volume connection: Application to rapid analyses of proteolytic digests. Anal. Chem., 71, 3036-3045
93. Zhang, B., Foret, F., and Karger, B.L. (2000) A microdevice with integrated liquid junction for facile peptide and protein analysis by capillary electrophoresis/electrospray mass spectrometry. Anal. Chem., 72: 1015-1022
94. Gustafsson, M., Hirschberg, D., Palmberg, C., Jornvall, H., and Bergman, T. (2004) Integrated sample preparation and MALDI mass spectrometry on a microfluidic compact disk. Anal. Chem., 76: 345-350
95. Wheeler, A.R., Moon, H., Kim, C.-J., Loo, J.A., and Garrell, R.L. (2004) Electrowetting-based microfluidics for analysis of peptides and proteins bymatrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem., 76: 4833-4838
96. Moon, H., Wheeler, A.R., Garrell, R.L., Loo, J.A., and Kim, C.J. (2006) An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS. Lab Chip, 6: 1213-1219
97. Ekstrom, S., Onnerfjord, P., Nilsson, J., Bengtsson, M., Laurell, T., and Marko-Varga, G. (2000) Integrated microanalytical technology enabling rapid and automated protein identification. Anal. Chem., 72, 286-293
98. Marko-Varga, G., Ekström, S., Helldin, G., Nilsson, J., and Laurell, T. (2001) Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption ionization-time-of-flight mass spectrometry: I. Microstructure development and manufacturing. Electrophoresis, 22: 3978-3983 (Pubitemid 33040217)
99. Ekström, S., Nilsson, J., Helldin, G., Laurell, T., and Marko-Varga, G. (2001) Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption ionization-time-of-flight mass spectrometry: II. Biological applications, Electrophoresis, 22: 3984-3992
100. Schmalzing, D., Belenky, A., Novotny, M. A., Koutny, L., Salas-Solano, O., El-Difrawy, S., Adourian, A., Matsudaira, P., and Ehrlich, D. (2000) Microchip electrophoresis: A method for high-speed SNP detection. Nucl. Acids Res., 28: e43-e43
101. Wang, A.J., Xu, J.J., and Chen, H.Y. (2007) In-situ grafting hydrophilic polymer on chitosan modified poly (dimethylsiloxane) microchip for separation of biomolecules. J. Chromatogr. A, 1147: 120-126
102. Dang, F., Li, W., Zhang, L., Mohammad, J., Tatsuhiro, I., Kiwada, H., Kaji, N., Manabu, T., and Baba, Y. (2006) Electrophoretic behavior of plasmid DNA in the presence of various intercalating dyes. J. Chromatogr. A, 1118: 218-225
103. Zhang, J., Weidong, H., Liang, D., Fang, D., Chu, B., and Marcus, G. (2006) Designing polymer matrix for microchip-based double-stranded DNA capillary electrophoresis. J. Chromatogr. A, 1117: 219-227
104. Prest, J.E., Baldock, S.J., Day, P.J.R., Fielden, P.R., Goddard, N.J., and Bernard, J.T.B. (2007) Miniaturised isotachophoresis of DNA. J. Chromatogr. A, 1156: 154-159
105. Shadpour, H., Hupert, M.L., Patterson, D., Liu, C., Galloway, M., Stryjewski, W., Goettert, J., and Steven, A.S. (2007) Multichannel microchip electrophoresis device fabricated in polycarbonate with an integrated contact conductivity sensor array. Anal. Chem., 79: 870-878
106. Mohammad, J., Ashraf, A.E., Xu, F., Ping, G., Maged, F., Shinka, T., Nakahori, Y., Ishikawa, M., and Baba, Y. (2005) Ultrafast diagnosis of the genetic-related disorders using the combined technologies of multiplex PCR and multichannel microchip electrophoresis. Anal. Sci., 21: 1537-1539
107. Mohammad, J., Ashraf, A.E., Maged, F., Fuquan, D., Guichen, P., Toshikatsu, S., Yutaka, N., Noritada, K., Manabu, T., and Baba, Y. (2006) Rapid multiplexing and simultaneous detection of human spermatogenetic failure with a 12 lane microchip electrophoresis system. Biol. Pharm. Bull., 29: 1487-1489
108. Kailasa, S.K., Lee, H.G., Yoo, D.J., and Kang, S.H. (2008) Ultra-fast detection and differentiation of Mycoplasma haemofelis and Candidatus M. Haemominutum in Korean feral cats by microchip electrophoresis with programmed field strength gradients. Bull. Korean Chem. Soc., 29: 153-158
109. Kailasa, S.K., Lee, M. J., Park, J., and Kang, S.H. (2008) Microchip gel electrophoresis with programmed field strength gradients for ultra-fast detection of canine T-cell lymphoma in dogs. Talanta, 75: 49-55
110. Lee, H.G., Kumar, K.S., Soh, J.R., Cha, Y.S., and Kang, S.H. (2008) Ultrafast simultaneous detection of obesity-related coenzymes in mice using microchip electrophoresis with a LIF detector. Anal. Chim. Acta, 619: 94-100
111. Osafune, T., Nagata, H., and Baba, Y. (2004) Influence of the pH on separating DNA by high-speed microchip electrophoresis. Anal. Sci., 20: 971-974
112. Kopp, U.M., de Mello, A.J., and Manz, A. (1998) Chemical amplification: Continuous-flow PCR on a chip. Science, 280: 1046-1048
113. Tabuchi, M. and Baba, Y. (2005) A triple-injection method for microchip electrophoresis. Electrophoresis, 26: 376-382
114. Alonso, A., Albarran, C., Martin, P., Garcia, P., Capilla, J., Garc?́a, O., de la Rua, C., Izaguirre, N., Pereira, F., Pereira, L., Amorim, A., and Sancho, M. (2006) Usefulness of microchip electrophoresis for the analysis of mitochondrial DNA in forensic and ancient DNA studies. Electrophoresis, 27: 5101-5109
115. Laili, M., Melin, J., Mohamad, M. R., Yamada, K., Ohta, M., Kaji, N., Tokeshi, M., Nilsson, M., and Baba, Y. (2007) Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis. Chem. Lett., 36: 396-397
116. Huan, H. and Guohua, Z. (2006) Sieving matrices for DNA separation in capillary electrophoreis and microchip electrophoresis. Yaowu Fenxi Zazhi, 26: 138-143
117. Thomas, C.N., Karl, W.P., Meena, B., Patrick, M., Kashan, A. S., Edgar, G.D., Chang, L., and Anneliese, E.B. (2006) Self-associating block copolymer networks for microchip electrophoresis provide enhanced DNA separation via "Inchworm" chain dynamics. Anal. Chem., 78: 4409-4415
118. Mohamadi, M.R., Kataoka, M., Mahmoudian, L., Jabasini, M., Shinohara, Y., and Baba, Y. (2005) Analysis of sources of error in quantitation of purified DNA fragments and unpurified PCR products by DNA microchip electrophoresis. Chromatographia, 61: 339-344
119. Xie, H., Li, B., Zhong, R., Qin, J., Zhu, Y., and Lin, B. (2008) Microfluidic device for integrated restriction digestion reaction and resulting DNA fragment analysis. Electrophoresis, 29: 4956-4963
120. Wang, L., Liu, D., Chen, H., and Zhou, X. (2008) A simple and sensitive transient ITP method for on-chip analysis of PCR samples. Electrophoresis, 29: 4976-4983
121. Tian, H., Brody, L. C., Fan, S., Huang, Z., Landers, J. P. (2001) Capillary and microchip electrophoresis for rapid detection of known mutations bycombining allele-specific DNA amplification with heteroduplex analysis. Clin. Chem., 47: 173-185
122. Hofgartner, T.W., Huhmer, F.R.A., Landers, P.J., and Kant, A.J. (1999) Rapid diagnosis of Herpes simplex encephalitis using microchip electrophoresis of PCR products. Clin. Chem., 45: 2120-2128
123. Chen, Y.H., Wang, W.C., Young, K.C., Chang, T.T., and Chen, S.H. (1999) Plastic microchip electrophoresis for analysis of PCR products of Hepatitis C virus. Clin. Chem., 45: 1938-1943
124. Sung, W.C., Lee, B.G., Tzeng, C.C., and Chen, S.H. (2001) Plastic microchip electrophoresis for genetic screening: The analysis of polymerase chain reactions products of fragile X (CGG)n alleles. Electrophoresis, 22: 1188-1193
125. Ferrance, J. and Landers, J.P. (2001) Exploiting sensitive laser-induced fluorescence detection on electrophoretic microchips for executing rapid clinical diagnostics. Luminescence, 16: 79-88
126. Cantafora, A., Blotta, I., Rivabene, R., Pisciotta, L., and Bertolini, S. (2003) Evaluation of RNA messengers involved in lipid trafficking of human intestinal cells by reverse-transcription polymerase chain reaction with competimer technology and microchip electrophoresis. Electrophoresis, 24: 3748-3754
127. Thomas, G., Sinville, R., Sutton, S., Farquar, H., Hammer, R.P., Soper, S.A., Cheng, Y. W., and Francis, B. (2004) Capillary and microelectrophoretic separations of ligase detection reaction products produced from low-abundant point mutations in genomic DNA. Electrophoresis, 25: 1668-1677
128. Ceriotti, L., Shibata, T., Folmer, B., Weiller, B.H., Roberts, M.A., de Rooij, N.F., and Elisabeth, V. (2002) Low-density lipoprotein analysis in microchip capillary electrophoresis systems. Electrophoresis, 23: 3615-3622
129. Pasas, S.A., Lacher, N.A., Davies, M.I., and Lunte, S.M. (2002) Detection of homocysteine by conventional and microchip capillary electrophoresis/ electrochemistry. Electrophoresis, 23: 759-766
130. Weiller, B.H., Ceriotti, L., Shibata, T., Rein, D., Roberts, M. A., Lichtenberg, J., German, J.B., de Rooij, N.F., and Elisabeth, V. (2002) Analysis of lipoproteins by capillary zone electrophoresis in microfluidic devices: Assay development and surface roughness measurements. Anal. Chem., 74: 1702-1711
131. Karasawa, K., Arakawa, H., Igarashi, T., Goto, N., and Maeda, M. (2004) Detection of cariogenic bacterial genes by microchip electrophoresis. J. Chromatogr. B, 810: 41-44
132. Lagally, E.T., Scherer, J.R., Blazej, R.G., Toriello, N.M., Diep, B.A., Ramchandani, M., Sensabaugh, G.F., Riley, L.W., and Mathies, R.A. (2004) Integrated portable genetic analysis microsystem for pathogen/ infectious disease detection. Anal. Chem., 76: 3162-3170
133. Koh, C.G., Tan, W., Zhao, M.Q., Ricco, A.J., and Fan, Z.H. (2003) Integrating polymerase chain reaction, valving, and electrophoresis in a plastic device for bacterial detection. Anal. Chem., 75: 4591-4598
134. Woolley, A.T., Hadley, D., Landre, P., deMello, A.J., Mathies, R.A., and Northrup, M.A. (1996) Functional integration of PCR amplification andcapillary electrophoresis in a microfabricated DNA analysis device. Anal. Chem., 68: 4081-4086
135. Waters, L.C., Jacobson, S.C., Kroutchinina, N., Khandurina, J., Foote, R.S., and Ramsey, J. M. (1998) Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing. Anal. Chem., 70:158-162
136. Waters, L.C., Jacobson, S.C., Kroutchinina, N., Khandurina, J., Foote, R.S., and Ramsey, J.M. (1998) Multiple sample PCR amplification and electrophoretic analysis on a microchip. Anal. Chem., 70: 5172-5176
137. Song, J.M., Mobley, J., and Tuan, V.D. (2003) Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip with capillary array electrophoresis. J. Chromatogr. B, 783: 501-508
138. Ertl, P., Emrich, C.A., Singhal, P., and Mathies, R.A. (2004) Capillary electrophoresis chips with a sheath-flow supported electrochemical detection system. Anal. Chem., 76: 3749-3755
139. Raisi, F., Blizard, B.A., Shabari, A.R., Ching, J., Kintz, G.J., Mitchell, J., Asuncion, L., Taylor, M.T., Weir, F., Western, L., Wong, W., Joshi, R., Howland, P., Chauhan, A., Peter, N., and Petersen, K.E. (2004) Human genomic DNA analysis using a semi-automated sample preparation, amplification, and electrophoresis separation platform. J. Sep. Sci., 27: 275-283
140. Ferrance, J.P., Wu, Q., Giordano, B., Hernandez, C., Kwok, Y., Snow, K., Steve, T., and Landers, J.P. (2003) Developments toward a complete micrototal analysis system for Duchenne muscular dystrophy diagnosis. Anal. Chim. Acta, 500: 223-236
141. Sanders, J.C., Breadmore, M.C., Mitchell, P.S., and Landers, J.P. (2002) A simple PDMS-based electro-fluidic interface for microchip electrophoretic separations. Analyst, 127: 1558-1563
142. Ferrance, J., Snow, K., and Landers, J.P. (2002) Evaluation of microchip electrophoresis as a molecular diagnostic method for Duchenne muscular dystrophy. Clin. Chem., 48: 380-383
143. Wang, W., Ni, K., and Zhou, G. (2006) Microchip electrophoresis coupled with multiplex polymerase chain reaction for typing multiple single nucleotide polymorphisms simultaneously. Fenxi Huaxue, 34: 1389-1394
144. Sinuhe, H., Bernhard, Z., Wolfgang, H., and Ying, L. (2005) Non-invasive detection of fetal gentics traits by analyzing extracellular DNAs in blood samples, Eur. Pat. Appl., (EP 1524321), p. 9-9
145. Feng, X., Mohammad, J., Bingmei, Z., Ling, Y., Xuezhi, C., Ahihiro, A., and Baba, Y. (2004) Single-step quantitation of DNA in microchip electrophoresis with linear imaging UV detection and fluorescence detection through co-migration with a digest, J. Chromatogr. A, 1051: 147-153
146. Munro, J.N., Snow, K., Kant, A.J., and Landers, P.J. (1999) Molecular diagnostics on microfabricated electrophoretic devices: From slab gel to capillary to microchip based assays for T-and B-cell lymphoproliferative disorders. Clin. Chem., 45: 1906-1917
147. Stenirri, S., Cretich, M., Rech, I., Restelli, A., Ghioni, M., Cova, S., Ferrari, M., Cremonesi, L., and Chiari, M. (2008). Dual-color microchip electrophoresis with single-photon avalanche diodes: Application to mutation detection. Electrophoresis, 29: 4972-4975
148. Chen, X., Tang, K., Lee, M., and Flynn, G.C. (2008) Microchip assays for screening monoclonal antibody product quality. Electrophoresis, 29: 4993-5002
149. Yang, Q., Zhao, Y.C., Xiong, Q., and Cheng, J. (2008) Rapid chip-based capillary electrophoretic mobility shift assay with negative pressure injection for the binding study of transcription factor Abf1 in Saccharomyces cerevisiae. Electrophoresis, 29: 5003-5009
150. Kartalov, E.P., Lin, H.D., Lee, T.D., Anderson, F.W., Taylor, R.C., and Scherer, A. (2008) Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices. Electrophoresis, 29: 5010-5016
151. Yu, L., Huang, H., Dong, X., Wu, D., Qin, J., and Lin, B. (2008) Simple, fast and high-throughput single-cell analysis on PDMS microfluidic chips. Electrophoresis, 29, 5055-5060
152. Umemoto, Y., Kataoka, M., Yatsushiro, S., Watanabe, M., Kido, J.I., Kakuhata, R., Yamamoto, T., Shinohara, Y., and Baba, Y. (2009) Sequential analysis of RNA synthesis by microchip electrophoresis. Anal. Biochem., 388: 161-163
153. Li, Y., Li, Y., Zheng, B., Qu, L., and Li. C. (2009) Determination of foodborne pathogenic bacteria by multiplex PCR-microchip capillary electrophoresis with genetic algorithm-support vector regression optimization. Anal. Chim. Acta, 643: 100-107
154. Shim, J., Dutta, P., and Ivory, F.C. (2009) Dispersion of protein bands in a horseshoe microchannel during IEF. Electrophoresis, 30: 723-731
155. Wang, W.P., Zhang, R.H., Wu, P., Wang, S., and Li, R. (2009) Estimation of allele frequency in pooled DNA by using PCR-RFLP combined with microchip electrophoresis. J. Chromatogr. B, 877: 1603-1606
156. Osiri, J.K., Shadpour, H., Park, S., Snowden, B.C., Chen, Z.Y., and Soper, S.A. (2008) Generating high peak capacity 2-D maps of complex proteomes using PMMA microchip electrophoresis. Electrophoresis, 29, 4984-4992
157. Huska, D., Hubalek, J., Adam, V., and Kizek, R. (2008) Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR. Electrophoresis, 29: 4964-4971