-
1
-
-
0030614426
-
The LightCycler: A microvolume multisample fluorimeter with rapid temperature control
-
Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA, Balis UJ: The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 1997, 22:176-181
-
(1997)
Biotechniques
, vol.22
, pp. 176-181
-
-
Wittwer, C.T.1
Ririe, K.M.2
Andrew, R.V.3
David, D.A.4
Gundry, R.A.5
Balis, U.J.6
-
2
-
-
69249147897
-
ACMG/CAP Molecular Genetics Committee
-
Participant Summary Report: MGL-B. ACMG/CAP Molecular Genetics Committee, 2006, pp 4-6
-
(2006)
Participant Summary Report: MGL-B
, pp. 4-6
-
-
-
3
-
-
0031022694
-
Continuous fluorescence monitoring of rapid cycle DNA amplification
-
Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP: Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997, 22:130-138
-
(1997)
Biotechniques
, vol.22
, pp. 130-138
-
-
Wittwer, C.T.1
Herrmann, M.G.2
Moss, A.A.3
Rasmussen, R.P.4
-
4
-
-
0013351171
-
Real-time PCR
-
Edited by Persing DH, Tenover FC, Versalovic J, Tang YW, Unger ER, Relman DA, White TJ. Washington DC, ASM Press
-
Wittwer CT, Kusukawa N: Real-time PCR. Diagnostic Molecular Microbiology; Principles and Applications. Edited by Persing DH, Tenover FC, Versalovic J, Tang YW, Unger ER, Relman DA, White TJ. Washington DC, ASM Press, 2004, pp 71-84
-
(2004)
Diagnostic Molecular Microbiology; Principles and Applications
, pp. 71-84
-
-
Wittwer, C.T.1
Kusukawa, N.2
-
5
-
-
0013351171
-
Real-time PCR and melting analysis
-
ed 2. Edited by Persing DH, Tenover FC, Hayden R, Nolte F, Tang YW, Belkum AV. Washington, DC, ASM Press, in press
-
Wittwer CT, Kusukawa N: Real-time PCR and melting analysis. Molecular Microbiology: Diagnostic Principles and Practice, ed 2. Edited by Persing DH, Tenover FC, Hayden R, Nolte F, Tang YW, Belkum AV. Washington, DC, ASM Press, 2009 (in press)
-
(2009)
Molecular Microbiology: Diagnostic Principles and Practice
-
-
Wittwer, C.T.1
Kusukawa, N.2
-
6
-
-
0031568919
-
Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
-
Ririe KM, Rasmussen RP, Wittwer CT: Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997, 245:154-160
-
(1997)
Anal Biochem
, vol.245
, pp. 154-160
-
-
Ririe, K.M.1
Rasmussen, R.P.2
Wittwer, C.T.3
-
7
-
-
0031451539
-
Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR
-
Lay MJ, Wittwer CT: Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR. Clin Chem 1997, 43:2262-2267
-
(1997)
Clin Chem
, vol.43
, pp. 2262-2267
-
-
Lay, M.J.1
Wittwer, C.T.2
-
8
-
-
0028834086
-
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
-
Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K: Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 1995, 4:357-362
-
(1995)
PCR Methods Appl
, vol.4
, pp. 357-362
-
-
Livak, K.J.1
Flood, S.J.2
Marmaro, J.3
Giusti, W.4
Deetz, K.5
-
9
-
-
0029957181
-
Real time quantitative PCR
-
Heid CA, Stevens J, Livak KJ, Williams PM: Real time quantitative PCR. Genome Res 1996, 6:986-994
-
(1996)
Genome Res
, vol.6
, pp. 986-994
-
-
Heid, C.A.1
Stevens, J.2
Livak, K.J.3
Williams, P.M.4
-
10
-
-
85031348658
-
The MIQE guidelines: Minimal information for publication of quantitative real-time PCR experiments
-
in press
-
Bustin S, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl M, Shipley G, Vandesompele J, Wittwer C: The MIQE guidelines: minimal information for publication of quantitative real-time PCR experiments. Clin Chem (in press)
-
Clin Chem
-
-
Bustin, S.1
Garson, J.2
Hellemans, J.3
Huggett, J.4
Kubista, M.5
Mueller, R.6
Nolan, T.7
Pfaffl, M.8
Shipley, G.9
Vandesompele, J.10
Wittwer, C.11
-
11
-
-
0033006003
-
Allelic discrimination using fluorogenic probes and the 5′ nuclease assay
-
Livak KJ: Allelic discrimination using fluorogenic probes and the 5′ nuclease assay. Genet Anal 1999, 14:143-149
-
(1999)
Genet Anal
, vol.14
, pp. 143-149
-
-
Livak, K.J.1
-
12
-
-
0002940799
-
Rapid cycle DNA amplification
-
Edited by Mulls K, Ferre F, Gibbs R. Deerfield Beach, Springer-Verlag
-
Wittwer CT, Reed GB, Ririe KM: Rapid cycle DNA amplification. The Polymerase Chain Reaction. Edited by Mulls K, Ferre F, Gibbs R. Deerfield Beach, Springer-Verlag 1994, pp 174-181
-
(1994)
The Polymerase Chain Reaction
, pp. 174-181
-
-
Wittwer, C.T.1
Reed, G.B.2
Ririe, K.M.3
-
13
-
-
0025326285
-
Minimizing the time required for DNA amplification by efficient heat transfer to small samples
-
Wittwer CT, Fillmore GC, Garling DJ: Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal Biochem 1990, 186:328-331
-
(1990)
Anal Biochem
, vol.186
, pp. 328-331
-
-
Wittwer, C.T.1
Fillmore, G.C.2
Garling, D.J.3
-
14
-
-
0025965114
-
Rapid cycle DNA amplification: Time and temperature optimization
-
Wittwer CT, Garling DJ: Rapid cycle DNA amplification: time and temperature optimization. Biotechniques 1991, 10:76-83
-
(1991)
Biotechniques
, vol.10
, pp. 76-83
-
-
Wittwer, C.T.1
Garling, D.J.2
-
15
-
-
66349108233
-
Rapid PCR and melting analysis
-
Edited by Bustin SA. Cambridge, Cambridge University Press, in press
-
Wittwer CT, Rasmussen RP, Ririe KM: Rapid PCR and melting analysis. The PCR Revolution. Edited by Bustin SA. Cambridge, Cambridge University Press, 2009 (in press)
-
(2009)
The PCR Revolution
-
-
Wittwer, C.T.1
Rasmussen, R.P.2
Ririe, K.M.3
-
16
-
-
0033766255
-
Influence of DNA target melting behavior on real-time PCR quantification
-
Wilhelm J, Hahn M, Pingoud A: Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem 2000, 46:1738-1743
-
(2000)
Clin Chem
, vol.46
, pp. 1738-1743
-
-
Wilhelm, J.1
Hahn, M.2
Pingoud, A.3
-
17
-
-
0031695438
-
Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes
-
Bernard PS, Ajioka RS, Kushner JP, Wittwer CT: Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes. Am J Pathol 1998, 153:1055-1061
-
(1998)
Am J Pathol
, vol.153
, pp. 1055-1061
-
-
Bernard, P.S.1
Ajioka, R.S.2
Kushner, J.P.3
Wittwer, C.T.4
-
18
-
-
0037098943
-
Simultaneous detection of methylenetetrahydrofolate reductase gene polymorphisms, C677T and A1298C, by melting curve analysis with LightCycler
-
Nakamura S, Aoshima T, Ikeda M, Sekido Y, Shimokata K, Niwa T: Simultaneous detection of methylenetetrahydrofolate reductase gene polymorphisms, C677T and A1298C, by melting curve analysis with LightCycler. Anal Biochem 2002, 306:340-343
-
(2002)
Anal Biochem
, vol.306
, pp. 340-343
-
-
Nakamura, S.1
Aoshima, T.2
Ikeda, M.3
Sekido, Y.4
Shimokata, K.5
Niwa, T.6
-
19
-
-
0033934164
-
Simultaneous detection of C282Y and H63D hemochromatosis mutations by dual-color probes
-
Phillips M, Meadows CA, Huang MY, Millson A, Lyon E: Simultaneous detection of C282Y and H63D hemochromatosis mutations by dual-color probes. Mol Diagn 2000, 5:107-116
-
(2000)
Mol Diagn
, vol.5
, pp. 107-116
-
-
Phillips, M.1
Meadows, C.A.2
Huang, M.Y.3
Millson, A.4
Lyon, E.5
-
20
-
-
0033762202
-
Genotyping of eight thiopurine methyltransferase mutations: Three-color multiplexing, "two-color/ shared" anchor, and fluorescence-quenching hybridization probe assays based on thermodynamic nearest-neighbor probe design
-
Schütz E, von Ahsen N, Oellerich M: Genotyping of eight thiopurine methyltransferase mutations: three-color multiplexing, "two-color/ shared" anchor, and fluorescence-quenching hybridization probe assays based on thermodynamic nearest-neighbor probe design. Clin Chem 2000, 46:1728-1737
-
(2000)
Clin Chem
, vol.46
, pp. 1728-1737
-
-
Schütz, E.1
von Ahsen, N.2
Oellerich, M.3
-
21
-
-
0036707748
-
Detection of a novel point mutation of the prothrombin gene at position 20209
-
Warshawsky I, Hren C, Sercia L, Shadrach B, Deitcher SR, Newton E, Kottke-Marchant K: Detection of a novel point mutation of the prothrombin gene at position 20209. Diagn Mol Pathol 2002, 11:152-156
-
(2002)
Diagn Mol Pathol
, vol.11
, pp. 152-156
-
-
Warshawsky, I.1
Hren, C.2
Sercia, L.3
Shadrach, B.4
Deitcher, S.R.5
Newton, E.6
Kottke-Marchant, K.7
-
22
-
-
33748751195
-
Functional analysis of two prothrombin 3′-untranslated region variants: The C20209T variant, mainly found among African-Americans, and the C20209A variant
-
van der Putten HH, Spaargaren-van Riel CC, Bertina RM, Vos HL: Functional analysis of two prothrombin 3′-untranslated region variants: the C20209T variant, mainly found among African-Americans, and the C20209A variant. J Thromb Haemost 2006, 4:2285-2287
-
(2006)
J Thromb Haemost
, vol.4
, pp. 2285-2287
-
-
van der Putten, H.H.1
Spaargaren-van Riel, C.C.2
Bertina, R.M.3
Vos, H.L.4
-
23
-
-
23044474418
-
Atypical melting curve resulting from genetic variation in the 3′ untranslated region at position 20218 in the prothrombin gene analyzed with the LightCycler factor II (prothrombin) G20210A assay
-
Tag CG, Schifflers MC, Mohnen M, Gressner AM, Weiskirchen R: Atypical melting curve resulting from genetic variation in the 3′ untranslated region at position 20218 in the prothrombin gene analyzed with the LightCycler factor II (prothrombin) G20210A assay. Clin Chem 2005, 51:1560-1561
-
(2005)
Clin Chem
, vol.51
, pp. 1560-1561
-
-
Tag, C.G.1
Schifflers, M.C.2
Mohnen, M.3
Gressner, A.M.4
Weiskirchen, R.5
-
24
-
-
0035559697
-
Mutation detection using fluorescent hybridization probes and melting curve analysis
-
Lyon E: Mutation detection using fluorescent hybridization probes and melting curve analysis. Expert Rev Mol Diagn 2001, 1:92-101
-
(2001)
Expert Rev Mol Diagn
, vol.1
, pp. 92-101
-
-
Lyon, E.1
-
25
-
-
23044481935
-
Discovering rare variants by use of melting temperature shifts seen in melting curve analysis
-
Lyon E: Discovering rare variants by use of melting temperature shifts seen in melting curve analysis. Clin Chem 2005, 51:1331-1332
-
(2005)
Clin Chem
, vol.51
, pp. 1331-1332
-
-
Lyon, E.1
-
26
-
-
0032437725
-
Detection and identification of base alterations within the region of factor V Leiden by fluorescent melting curves
-
Lyon E, Millson A, Phan T, Wittwer CT: Detection and identification of base alterations within the region of factor V Leiden by fluorescent melting curves. Mol Diagn 1998, 3:203-210
-
(1998)
Mol Diagn
, vol.3
, pp. 203-210
-
-
Lyon, E.1
Millson, A.2
Phan, T.3
Wittwer, C.T.4
-
27
-
-
23044451424
-
Factor V null mutation affecting the Roche LightCycler factor V Leiden assay
-
Mahadevan MS, Benson PV: Factor V null mutation affecting the Roche LightCycler factor V Leiden assay. Clin Chem 2005, 51:1533-1535
-
(2005)
Clin Chem
, vol.51
, pp. 1533-1535
-
-
Mahadevan, M.S.1
Benson, P.V.2
-
28
-
-
0035283211
-
Fluorescein-labeled oligonucleotides for real-time PCR: Using the inherent quenching of deoxyguanosine nucleotides
-
Crockett AO, Wittwer CT: Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides. Anal Biochem 2001, 290:89-97
-
(2001)
Anal Biochem
, vol.290
, pp. 89-97
-
-
Crockett, A.O.1
Wittwer, C.T.2
-
29
-
-
0037970057
-
Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: A novel strategy for PCR detection and genotyping
-
Vaughn CP, Elenitoba-Johnson KS: Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: a novel strategy for PCR detection and genotyping. Am J Pathol 2003, 163:29-35
-
(2003)
Am J Pathol
, vol.163
, pp. 29-35
-
-
Vaughn, C.P.1
Elenitoba-Johnson, K.S.2
-
30
-
-
46649090575
-
Warfarin dose and INR related to genotypes of CYP2C9 and VKORC1 in patients with myocardial infarction
-
Haug KB, Sharikabad MN, Kringen MK, Narum S, Sjaatil ST, Johansen PW, Kierulf P, Seljeflot I, Arnesen H, Brors O: Warfarin dose and INR related to genotypes of CYP2C9 and VKORC1 in patients with myocardial infarction. Thromb J 2008, 6:7
-
(2008)
Thromb J
, vol.6
, pp. 7
-
-
Haug, K.B.1
Sharikabad, M.N.2
Kringen, M.K.3
Narum, S.4
Sjaatil, S.T.5
Johansen, P.W.6
Kierulf, P.7
Seljeflot, I.8
Arnesen, H.9
Brors, O.10
-
31
-
-
36549030324
-
Randomized trial of genotype-guided versus standard warfarin dosing in patients initiating oral anticoagulation
-
Anderson JL, Horne BD, Stevens SM, Grove AS, Barton S, Nicholas ZP, Kahn SF, May HT, Samuelson KM, Muhlestein JB, Carlquist JF: Randomized trial of genotype-guided versus standard warfarin dosing in patients initiating oral anticoagulation. Circulation 2007, 116:2563-2570
-
(2007)
Circulation
, vol.116
, pp. 2563-2570
-
-
Anderson, J.L.1
Horne, B.D.2
Stevens, S.M.3
Grove, A.S.4
Barton, S.5
Nicholas, Z.P.6
Kahn, S.F.7
May, H.T.8
Samuelson, K.M.9
Muhlestein, J.B.10
Carlquist, J.F.11
-
32
-
-
3242742969
-
Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye
-
Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT: Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye. Clin Chem 2004, 50:1328-1335
-
(2004)
Clin Chem
, vol.50
, pp. 1328-1335
-
-
Zhou, L.1
Myers, A.N.2
Vandersteen, J.G.3
Wang, L.4
Wittwer, C.T.5
-
33
-
-
33644876042
-
Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis
-
Chou LS, Meadows C, Wittwer CT, Lyon E: Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis. Biotechniques 2005, 39:644-648
-
(2005)
Biotechniques
, vol.39
, pp. 644-648
-
-
Chou, L.S.1
Meadows, C.2
Wittwer, C.T.3
Lyon, E.4
-
34
-
-
25444491205
-
High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution
-
Zhou L, Wang L, Palais R, Pryor R, Wittwer CT: High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clin Chem 2005, 51:1770-1777
-
(2005)
Clin Chem
, vol.51
, pp. 1770-1777
-
-
Zhou, L.1
Wang, L.2
Palais, R.3
Pryor, R.4
Wittwer, C.T.5
-
35
-
-
34247874785
-
RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis
-
Margraf RL, Mao R, Highsmith WE, Holtegaard LM, Wittwer CT: RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis. J Mol Diagn 2007, 9:184-196
-
(2007)
J Mol Diagn
, vol.9
, pp. 184-196
-
-
Margraf, R.L.1
Mao, R.2
Highsmith, W.E.3
Holtegaard, L.M.4
Wittwer, C.T.5
-
36
-
-
34848862166
-
Unlabeled probes for the detection and typing of herpes simplex virus
-
Dames S, Pattison DC, Bromley LK, Wittwer CT, Voelkerding KV: Unlabeled probes for the detection and typing of herpes simplex virus. Clin Chem 2007, 53:1847-1854
-
(2007)
Clin Chem
, vol.53
, pp. 1847-1854
-
-
Dames, S.1
Pattison, D.C.2
Bromley, L.K.3
Wittwer, C.T.4
Voelkerding, K.V.5
-
37
-
-
34347378877
-
Identifying common genetic variants by high-resolution melting
-
Vandersteen JG, Bayrak-Toydemir P, Palais RA, Wittwer CT: Identifying common genetic variants by high-resolution melting. Clin Chem 2007, 53:1191-1198
-
(2007)
Clin Chem
, vol.53
, pp. 1191-1198
-
-
Vandersteen, J.G.1
Bayrak-Toydemir, P.2
Palais, R.A.3
Wittwer, C.T.4
-
38
-
-
52049113295
-
SNP genotyping by unlabeled probe melting analysis
-
Edited by Seitz O, Marx A. Totowa, Humana Press
-
Erali M, Palais R, Wittwer CT: SNP genotyping by unlabeled probe melting analysis. Molecular Beacons: Signalling Nucleic Acid Probes, Methods and Protocols. Edited by Seitz O, Marx A. Totowa, Humana Press, 2008, pp 199-206
-
(2008)
Molecular Beacons: Signalling Nucleic Acid Probes, Methods and Protocols
, pp. 199-206
-
-
Erali, M.1
Palais, R.2
Wittwer, C.T.3
-
39
-
-
54049108161
-
Snapback primer genotyping with saturating DNA dye and melting analysis
-
Zhou L, Errigo RJ, Lu H, Poritz MA, Seipp MT, Wittwer CT: Snapback primer genotyping with saturating DNA dye and melting analysis. Clin Chem 2008, 54:1648-1656
-
(2008)
Clin Chem
, vol.54
, pp. 1648-1656
-
-
Zhou, L.1
Errigo, R.J.2
Lu, H.3
Poritz, M.A.4
Seipp, M.T.5
Wittwer, C.T.6
-
40
-
-
33645224189
-
Masking selected sequence variation by incorporating mismatches into melting analysis probes
-
Margraf RL, Mao R, Wittwer CT: Masking selected sequence variation by incorporating mismatches into melting analysis probes. Hum Mutat 2006, 27:269-278
-
(2006)
Hum Mutat
, vol.27
, pp. 269-278
-
-
Margraf, R.L.1
Mao, R.2
Wittwer, C.T.3
-
41
-
-
0033046861
-
Single-tube genotyping without oligonucleotide probes
-
Germer S, Higuchi R: Single-tube genotyping without oligonucleotide probes. Genome Res 1999, 9:72-78
-
(1999)
Genome Res
, vol.9
, pp. 72-78
-
-
Germer, S.1
Higuchi, R.2
-
42
-
-
0038359432
-
High-resolution genotyping by amplicon melting analysis using LCGreen
-
Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ: High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003, 49:853-860
-
(2003)
Clin Chem
, vol.49
, pp. 853-860
-
-
Wittwer, C.T.1
Reed, G.H.2
Gundry, C.N.3
Vandersteen, J.G.4
Pryor, R.J.5
-
43
-
-
3042651880
-
Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons
-
Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E, Wittwer C: Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem 2004, 50:1156-1164
-
(2004)
Clin Chem
, vol.50
, pp. 1156-1164
-
-
Liew, M.1
Pryor, R.2
Palais, R.3
Meadows, C.4
Erali, M.5
Lyon, E.6
Wittwer, C.7
-
44
-
-
32944477875
-
Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes
-
Liew M, Nelson L, Margraf R, Mitchell S, Erali M, Mao R, Lyon E, Wittwer C: Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes. J Mol Diagn 2006, 8:97-104
-
(2006)
J Mol Diagn
, vol.8
, pp. 97-104
-
-
Liew, M.1
Nelson, L.2
Margraf, R.3
Mitchell, S.4
Erali, M.5
Mao, R.6
Lyon, E.7
Wittwer, C.8
-
45
-
-
34247872951
-
Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting
-
Liew M, Seipp M, Durtschi J, Margraf RL, Dames S, Erali M, Voelkerding K, Wittwer C: Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting. Am J Clin Pathol 2007, 127:341-348
-
(2007)
Am J Clin Pathol
, vol.127
, pp. 341-348
-
-
Liew, M.1
Seipp, M.2
Durtschi, J.3
Margraf, R.L.4
Dames, S.5
Erali, M.6
Voelkerding, K.7
Wittwer, C.8
-
46
-
-
45549101581
-
Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons
-
Gundry CN, Dobrowolski SF, Martin YR, Robbins TC, Nay LM, Boyd N, Coyne T, Wall MD, Wittwer CT, Teng DH: Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons. Nucleic Acids Res 2008, 36:3401-3408
-
(2008)
Nucleic Acids Res
, vol.36
, pp. 3401-3408
-
-
Gundry, C.N.1
Dobrowolski, S.F.2
Martin, Y.R.3
Robbins, T.C.4
Nay, L.M.5
Boyd, N.6
Coyne, T.7
Wall, M.D.8
Wittwer, C.T.9
Teng, D.H.10
-
47
-
-
26844517352
-
Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping
-
Palais RA, Liew MA, Wittwer CT: Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping. Anal Biochem 2005, 346:167-175
-
(2005)
Anal Biochem
, vol.346
, pp. 167-175
-
-
Palais, R.A.1
Liew, M.A.2
Wittwer, C.T.3
-
48
-
-
33644548649
-
Amplicon DNA melting analysis for mutation scanning and genotyping: Cross-platform comparison of instruments and dyes
-
Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV: Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes. Clin Chem 2006, 52:494-503
-
(2006)
Clin Chem
, vol.52
, pp. 494-503
-
-
Herrmann, M.G.1
Durtschi, J.D.2
Bromley, L.K.3
Wittwer, C.T.4
Voelkerding, K.V.5
-
49
-
-
0034949833
-
Limitations of genotyping based on amplicon melting temperature
-
von Ahsen N, Oellerich M, Schutz E: Limitations of genotyping based on amplicon melting temperature. Clin Chem 2001, 47:1331-1332
-
(2001)
Clin Chem
, vol.47
, pp. 1331-1332
-
-
von Ahsen, N.1
Oellerich, M.2
Schutz, E.3
-
50
-
-
23944500622
-
Direct molecular haplotyping by melting curve analysis of hybridization probes: Beta 2-adrenergic receptor haplotypes as an example
-
Pont-Kingdon G, Lyon E: Direct molecular haplotyping by melting curve analysis of hybridization probes: beta 2-adrenergic receptor haplotypes as an example. Nucleic Acids Res 2005, 33:e89
-
(2005)
Nucleic Acids Res
, vol.33
-
-
Pont-Kingdon, G.1
Lyon, E.2
-
51
-
-
44849123472
-
Design and application of noncontinuously binding probes used for haplotyping and genotyping
-
Pont-Kingdon G, Margraf RL, Sumner K, Millson A, Lyon E, Schutz E: Design and application of noncontinuously binding probes used for haplotyping and genotyping. Clin Chem 2008, 54:990-999
-
(2008)
Clin Chem
, vol.54
, pp. 990-999
-
-
Pont-Kingdon, G.1
Margraf, R.L.2
Sumner, K.3
Millson, A.4
Lyon, E.5
Schutz, E.6
-
52
-
-
35648961269
-
Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis
-
Montgomery J, Wittwer CT, Kent JO, Zhou L: Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis. Clin Chem 2007, 53:1891-1898
-
(2007)
Clin Chem
, vol.53
, pp. 1891-1898
-
-
Montgomery, J.1
Wittwer, C.T.2
Kent, J.O.3
Zhou, L.4
-
53
-
-
34848845055
-
Epidermal growth factor receptor mutation detection using high-resolution melting analysis predicts outcomes in patients with advanced non small cell lung cancer treated with gefitinib
-
Takano T, Ohe Y, Tsuta K, Fukui T, Sakamoto H, Yoshida T, Tateishi U, Nokihara H, Yamamoto N, Sekine I, Kunitoh H, Matsuno Y, Furuta K, Tamura T: Epidermal growth factor receptor mutation detection using high-resolution melting analysis predicts outcomes in patients with advanced non small cell lung cancer treated with gefitinib. Clin Cancer Res 2007, 13:5385-5390
-
(2007)
Clin Cancer Res
, vol.13
, pp. 5385-5390
-
-
Takano, T.1
Ohe, Y.2
Tsuta, K.3
Fukui, T.4
Sakamoto, H.5
Yoshida, T.6
Tateishi, U.7
Nokihara, H.8
Yamamoto, N.9
Sekine, I.10
Kunitoh, H.11
Matsuno, Y.12
Furuta, K.13
Tamura, T.14
-
54
-
-
48649092117
-
High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer
-
Simi L, Pratesi N, Vignoli M, Sestini R, Cianchi F, Valanzano R, Nobili S, Mini E, Pazzagli M, Orlando C: High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer. Am J Clin Pathol 2008, 130:247-253
-
(2008)
Am J Clin Pathol
, vol.130
, pp. 247-253
-
-
Simi, L.1
Pratesi, N.2
Vignoli, M.3
Sestini, R.4
Cianchi, F.5
Valanzano, R.6
Nobili, S.7
Mini, E.8
Pazzagli, M.9
Orlando, C.10
-
55
-
-
34547885048
-
High-resolution melting amplicon analysis as a method to detect c-kit and platelet-derived growth factor receptor alpha activating mutations in gastrointestinal stromal tumors
-
Holden JA, Willmore-Payne C, Coppola D, Garrett CR, Layfield LJ: High-resolution melting amplicon analysis as a method to detect c-kit and platelet-derived growth factor receptor alpha activating mutations in gastrointestinal stromal tumors. Am J Clin Pathol 2007, 128:230-238
-
(2007)
Am J Clin Pathol
, vol.128
, pp. 230-238
-
-
Holden, J.A.1
Willmore-Payne, C.2
Coppola, D.3
Garrett, C.R.4
Layfield, L.J.5
-
56
-
-
3242794909
-
Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis
-
Willmore C, Holden JA, Zhou L, Tripp S, Wittwer CT, Layfield LJ: Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis. Am J Clin Pathol 2004, 122:206-216
-
(2004)
Am J Clin Pathol
, vol.122
, pp. 206-216
-
-
Willmore, C.1
Holden, J.A.2
Zhou, L.3
Tripp, S.4
Wittwer, C.T.5
Layfield, L.J.6
-
57
-
-
20144378490
-
Human malignant melanoma: Detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis
-
Willmore-Payne C, Holden JA, Tripp S, Layfield LJ: Human malignant melanoma: detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis. Hum Pathol 2005, 36:486-493
-
(2005)
Hum Pathol
, vol.36
, pp. 486-493
-
-
Willmore-Payne, C.1
Holden, J.A.2
Tripp, S.3
Layfield, L.J.4
-
58
-
-
42949168884
-
High-throughput amplicon scanning of the TP53 gene in breast cancer using high-resolution fluorescent melting curve analyses and automatic mutation calling
-
Bastien R, Lewis TB, Hawkes JE, Quackenbush JF, Robbins TC, Palazzo J, Perou CM, Bernard PS: High-throughput amplicon scanning of the TP53 gene in breast cancer using high-resolution fluorescent melting curve analyses and automatic mutation calling. Hum Mutat 2008, 29:757-764
-
(2008)
Hum Mutat
, vol.29
, pp. 757-764
-
-
Bastien, R.1
Lewis, T.B.2
Hawkes, J.E.3
Quackenbush, J.F.4
Robbins, T.C.5
Palazzo, J.6
Perou, C.M.7
Bernard, P.S.8
-
59
-
-
84902029237
-
High resolution melting curve analysis for molecular diagnostics
-
ed 2. Edited by Patrinos GP, Ansorge W. Burlington, Elsevier, in press
-
Farrar JS, Reed GH, Wittwer CT: High resolution melting curve analysis for molecular diagnostics. Molecular Diagnostics, ed 2. Edited by Patrinos GP, Ansorge W. Burlington, Elsevier, 2009 (in press)
-
(2009)
Molecular Diagnostics
-
-
Farrar, J.S.1
Reed, G.H.2
Wittwer, C.T.3
-
60
-
-
67349100169
-
High-resolution melting analysis for rapid screening of BRCA1 and BRCA2 Spanish mutations
-
doi 10.1007/s10549-008-0073-7
-
De Juan I, Esteban E, Palanca S, Barragan E, Bolufer P: High-resolution melting analysis for rapid screening of BRCA1 and BRCA2 Spanish mutations. Breast Cancer Res Treat doi 10.1007/s10549-008-0073-7
-
Breast Cancer Res Treat
-
-
De Juan, I.1
Esteban, E.2
Palanca, S.3
Barragan, E.4
Bolufer, P.5
-
61
-
-
44849123232
-
Rapid and sensitive detection of BRCA1/2 mutations in a diagnostic setting: Comparison of two high-resolution melting platforms
-
De Leeneer K, Coene I, Poppe B, De Paepe A, Claes K: Rapid and sensitive detection of BRCA1/2 mutations in a diagnostic setting: comparison of two high-resolution melting platforms. Clin Chem 2008, 54:982-989
-
(2008)
Clin Chem
, vol.54
, pp. 982-989
-
-
De Leeneer, K.1
Coene, I.2
Poppe, B.3
De Paepe, A.4
Claes, K.5
-
62
-
-
52249092845
-
Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene
-
Audrezet MP, Dabricot A, Le Marechal C, Ferec C: Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. J Mol Diagn 2008, 10:424 -434
-
(2008)
J Mol Diagn
, vol.10
, pp. 424-434
-
-
Audrezet, M.P.1
Dabricot, A.2
Le Marechal, C.3
Ferec, C.4
-
63
-
-
36649014064
-
Detection of factor VIII gene mutations by high-resolution melting analysis
-
Laurie AD, Smith MP, George PM: Detection of factor VIII gene mutations by high-resolution melting analysis. Clin Chem 2007, 53:2211-2214
-
(2007)
Clin Chem
, vol.53
, pp. 2211-2214
-
-
Laurie, A.D.1
Smith, M.P.2
George, P.M.3
-
64
-
-
46249098884
-
Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM
-
Lin SY, Su YN, Hung CC, Tsay W, Chiou SS, Chang CT, Ho HN, Lee CN: Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM. BMC Med Genet 2008, 9:53
-
(2008)
BMC Med Genet
, vol.9
, pp. 53
-
-
Lin, S.Y.1
Su, Y.N.2
Hung, C.C.3
Tsay, W.4
Chiou, S.S.5
Chang, C.T.6
Ho, H.N.7
Lee, C.N.8
-
65
-
-
33847407062
-
Mutation scanning the GJB1 gene with high-resolution melting analysis: Implications for mutation scanning of genes for Charcot-Marie-Tooth disease
-
Kennerson ML, Warburton T, Nelis E, Brewer M, Polly P, De Jonghe P, Timmerman V, Nicholson GA: Mutation scanning the GJB1 gene with high-resolution melting analysis: implications for mutation scanning of genes for Charcot-Marie-Tooth disease. Clin Chem 2007, 53:349-352
-
(2007)
Clin Chem
, vol.53
, pp. 349-352
-
-
Kennerson, M.L.1
Warburton, T.2
Nelis, E.3
Brewer, M.4
Polly, P.5
De Jonghe, P.6
Timmerman, V.7
Nicholson, G.A.8
-
66
-
-
34249877249
-
Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis
-
Montgomery J, Wittwer CT, Palais RA, Zhou L: Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nat Prot 2007, 2:59-66
-
(2007)
Nat Prot
, vol.2
, pp. 59-66
-
-
Montgomery, J.1
Wittwer, C.T.2
Palais, R.A.3
Zhou, L.4
-
67
-
-
0034505142
-
Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using fluorescently labeled probes
-
Bolufer P, Sanz GF, Barragan E, Sanz MA, Cervera J, Lerma E, Senent L, Moreno I, Planelles MD: Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using fluorescently labeled probes. Haematologica 2000, 85:1248-1254
-
(2000)
Haematologica
, vol.85
, pp. 1248-1254
-
-
Bolufer, P.1
Sanz, G.F.2
Barragan, E.3
Sanz, M.A.4
Cervera, J.5
Lerma, E.6
Senent, L.7
Moreno, I.8
Planelles, M.D.9
-
68
-
-
0033168968
-
LightCycler technology for the quantitation of bcr/abl fusion transcripts
-
Kreuzer KA, Lass U, Bohn A, Landt O, Schmidt CA: LightCycler technology for the quantitation of bcr/abl fusion transcripts. Cancer Res 1999, 59:3171-3174
-
(1999)
Cancer Res
, vol.59
, pp. 3171-3174
-
-
Kreuzer, K.A.1
Lass, U.2
Bohn, A.3
Landt, O.4
Schmidt, C.A.5
-
69
-
-
34548718731
-
Inter-laboratory comparison of chronic myeloid leukemia minimal residual disease monitoring: Summary and recommendations
-
Zhang T, Grenier S, Nwachukwu B, Wei C, Lipton JH, Kamel-Reid S: Inter-laboratory comparison of chronic myeloid leukemia minimal residual disease monitoring: summary and recommendations. J Mol Diagn 2007, 9:421-430
-
(2007)
J Mol Diagn
, vol.9
, pp. 421-430
-
-
Zhang, T.1
Grenier, S.2
Nwachukwu, B.3
Wei, C.4
Lipton, J.H.5
Kamel-Reid, S.6
-
70
-
-
34347388416
-
Agreement in breast cancer classification between microarray and quantitative reverse transcription PCR from fresh-frozen and formalin-fixed, paraffin-embedded tissues
-
Mullins M, Perreard L, Quackenbush JF, Gauthier N, Bayer S, Ellis M, Parker J, Perou CM, Szabo A, Bernard PS: Agreement in breast cancer classification between microarray and quantitative reverse transcription PCR from fresh-frozen and formalin-fixed, paraffin-embedded tissues. Clin Chem 2007, 53:1273-1279
-
(2007)
Clin Chem
, vol.53
, pp. 1273-1279
-
-
Mullins, M.1
Perreard, L.2
Quackenbush, J.F.3
Gauthier, N.4
Bayer, S.5
Ellis, M.6
Parker, J.7
Perou, C.M.8
Szabo, A.9
Bernard, P.S.10
-
71
-
-
0034949862
-
In-tube DNA methylation profiling by fluorescence melting curve analysis
-
Worm J, Aggerholm A, Guldberg P: In-tube DNA methylation profiling by fluorescence melting curve analysis. Clin Chem 2001, 47:1183-1189
-
(2001)
Clin Chem
, vol.47
, pp. 1183-1189
-
-
Worm, J.1
Aggerholm, A.2
Guldberg, P.3
-
72
-
-
33745459305
-
Molecular diagnosis of Prader-Willi and Angelman syndromes by methylation-specific melting analysis and methylation-specific multiplex ligation-dependent probe amplification
-
Procter M, Chou LS, Tang W, Jama M, Mao R: Molecular diagnosis of Prader-Willi and Angelman syndromes by methylation-specific melting analysis and methylation-specific multiplex ligation-dependent probe amplification. Clin Chem 2006, 52:1276-1283
-
(2006)
Clin Chem
, vol.52
, pp. 1276-1283
-
-
Procter, M.1
Chou, L.S.2
Tang, W.3
Jama, M.4
Mao, R.5
-
73
-
-
0041333806
-
Application of rapid-cycle real-time polymerase chain reaction for diagnostic testing in the clinical microbiology laboratory
-
Cockerill III FR: Application of rapid-cycle real-time polymerase chain reaction for diagnostic testing in the clinical microbiology laboratory. Arch Pathol Lab Med 2003, 127:1112-1120
-
(2003)
Arch Pathol Lab Med
, vol.127
, pp. 1112-1120
-
-
Cockerill III, F.R.1
-
74
-
-
31544469804
-
Real-time PCR in clinical microbiology: Applications for routine laboratory testing
-
Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF, Vetter EA, Yao JD, Wengenack NL, Rosenblatt JE, Cockerill III FR, Smith TF: Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev 2006, 19:165-256
-
(2006)
Clin Microbiol Rev
, vol.19
, pp. 165-256
-
-
Espy, M.J.1
Uhl, J.R.2
Sloan, L.M.3
Buckwalter, S.P.4
Jones, M.F.5
Vetter, E.A.6
Yao, J.D.7
Wengenack, N.L.8
Rosenblatt, J.E.9
Cockerill III, F.R.10
Smith, T.F.11
-
75
-
-
0036708332
-
Rapid-cycle PCR and fluorimetry for detection of mycobacteria
-
Lachnik J, Ackermann B, Bohrssen A, Maass S, Diephaus C, Puncken A, Stermann M, Bange FC: Rapid-cycle PCR and fluorimetry for detection of mycobacteria. J Clin Microbiol 2002, 40:3364-3373
-
(2002)
J Clin Microbiol
, vol.40
, pp. 3364-3373
-
-
Lachnik, J.1
Ackermann, B.2
Bohrssen, A.3
Maass, S.4
Diephaus, C.5
Puncken, A.6
Stermann, M.7
Bange, F.C.8
-
76
-
-
0036135009
-
Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens
-
Sloan LM, Hopkins MK, Mitchell PS, Vetter EA, Rosenblatt JE, Harmsen WS, Cockerill FR, Patel R: Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens. J Clin Microbiol 2002, 40:96-100
-
(2002)
J Clin Microbiol
, vol.40
, pp. 96-100
-
-
Sloan, L.M.1
Hopkins, M.K.2
Mitchell, P.S.3
Vetter, E.A.4
Rosenblatt, J.E.5
Harmsen, W.S.6
Cockerill, F.R.7
Patel, R.8
-
77
-
-
43049121061
-
Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies
-
Mancini N, Clerici D, Diotti R, Perotti M, Ghidoli N, De Marco D, Pizzorno B, Emrich T, Burioni R, Ciceri F, Clementi M: Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 2008, 57:601-604
-
(2008)
J Med Microbiol
, vol.57
, pp. 601-604
-
-
Mancini, N.1
Clerici, D.2
Diotti, R.3
Perotti, M.4
Ghidoli, N.5
De Marco, D.6
Pizzorno, B.7
Emrich, T.8
Burioni, R.9
Ciceri, F.10
Clementi, M.11
-
78
-
-
47649115068
-
Confirmation of single exon deletions in MLH1 and MSH2 using quantitative polymerase chain reaction
-
Vaughn CP, Lyon E, Samowitz WS: Confirmation of single exon deletions in MLH1 and MSH2 using quantitative polymerase chain reaction. J Mol Diagn 2008, 10:355-360
-
(2008)
J Mol Diagn
, vol.10
, pp. 355-360
-
-
Vaughn, C.P.1
Lyon, E.2
Samowitz, W.S.3
-
79
-
-
33750430852
-
Detection of exon deletions within an entire gene (CFTR) by relative quantification on the LightCycler
-
Schneider M, Joncourt F, Sanz J, von Kanel T, Gallati S: Detection of exon deletions within an entire gene (CFTR) by relative quantification on the LightCycler. Clin Chem 2006, 52:2005-2012
-
(2006)
Clin Chem
, vol.52
, pp. 2005-2012
-
-
Schneider, M.1
Joncourt, F.2
Sanz, J.3
von Kanel, T.4
Gallati, S.5
-
80
-
-
0035043217
-
Quantification of HER2/neu gene amplification by competitive PCR using fluorescent melting curve analysis
-
Lyon E, Millson A, Lowery MC, Woods R, Wittwer CT: Quantification of HER2/neu gene amplification by competitive PCR using fluorescent melting curve analysis. Clin Chem 2001, 47:844-851
-
(2001)
Clin Chem
, vol.47
, pp. 844-851
-
-
Lyon, E.1
Millson, A.2
Lowery, M.C.3
Woods, R.4
Wittwer, C.T.5
-
81
-
-
0042065208
-
Comparison of two quantitative polymerase chain reaction methods for detecting HER2/neu amplification
-
Millson A, Suli A, Hartung L, Kunitake S, Bennett A, Nordberg MC, Hanna W, Wittwer CT, Seth A, Lyon E: Comparison of two quantitative polymerase chain reaction methods for detecting HER2/neu amplification. J Mol Diagn 2003, 5:184-190
-
(2003)
J Mol Diagn
, vol.5
, pp. 184-190
-
-
Millson, A.1
Suli, A.2
Hartung, L.3
Kunitake, S.4
Bennett, A.5
Nordberg, M.C.6
Hanna, W.7
Wittwer, C.T.8
Seth, A.9
Lyon, E.10
-
82
-
-
0038272057
-
Rapid detection of aneuploidy (trisomy 21) by allele quantification combined with melting curves analysis of single-nucleotide polymorphism loci
-
Pont-Kingdon G, Lyon E: Rapid detection of aneuploidy (trisomy 21) by allele quantification combined with melting curves analysis of single-nucleotide polymorphism loci. Clin Chem 2003, 49:1087-1094
-
(2003)
Clin Chem
, vol.49
, pp. 1087-1094
-
-
Pont-Kingdon, G.1
Lyon, E.2
|