Neutron and X-ray crystal structures of a perdeuterated enzyme inhibitor complex reveal the catalytic proton network of the Toho-1 β-lactamase for the acylation reaction

Journal of Biological Chemistry

Volumn 288, Issue 7, 2013, Pages 4715-4722

  Tomanicek, Stephen J ^a   Standaert, Robert F ^a,b   Weiss, Kevin L ^a   Ostermann, Andreas ^c   Schrader, Tobias E ^d   Ng, Joseph D ^e   Coates, Leighton ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF TENNESSEE   (United States)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^d FORSCHUNGSZENTRUM JÜLICH   (Germany)

^e Outstation at FRM II ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLATION REACTIONS; ANTIBIOTIC RESISTANCE; CATALYTIC MECHANISMS; ENZYME-INHIBITOR COMPLEX; GENERAL BASE; LACTAMASES; TRANSITION STATE; X RAY CRYSTAL STRUCTURES;

ANTIBIOTICS;

ACYLATION;

BETA LACTAMASE CTX M; BETA LACTAMASE TOHO 1; UNCLASSIFIED DRUG;

ACYLATION; ARTICLE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DEUTERIUM HYDROGEN EXCHANGE; ENZYME ACTIVE SITE; ENZYME INHIBITOR COMPLEX; ENZYME MECHANISM; HYDROGEN BOND; NEUTRON DIFFRACTION; PRIORITY JOURNAL; PROTEIN METABOLISM; PROTEIN PERDEUTERATION; PROTON TRANSPORT; SIGNAL DETECTION; STRUCTURE ANALYSIS; X RAY DIFFRACTION;

ACYLATION; BETA-LACTAMASES; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DRUG RESISTANCE, BACTERIAL; ENZYME INHIBITORS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN; HYDROGEN BONDING; LIGANDS; MODELS, CHEMICAL; MOLECULAR CONFORMATION; NEUTRONS; NITROGEN; PROTONS; THIOPHENES;

EID: 84874077280     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.436238     Document Type: Article

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