The active site protonation states of perdeuterated Toho-1 β-lactamase determined by neutron diffraction support a role for Glu166 as the general base in acylation

FEBS Letters

Volumn 585, Issue 2, 2011, Pages 364-368

  Tomanicek, Stephen J ^a   Wang, Kathy K ^a   Weiss, Kevin L ^a   Blakeley, Matthew P ^b   Cooper, Jonathan ^c   Chen, Yu ^d   Coates, Leighton ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b INSTITUT LAUE LANGEVIN   (France)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d UNIVERSITY OF SOUTH FLORIDA   (United States)

Author keywords

Lactamase; CTX M type ESBLs; Extended spectrum lactamases; Neutron diffraction; Perdeuterated neutron structure; Toho 1

Indexed keywords

ARGININE; ASPARAGINE; BETA LACTAMASE; BETA LACTAMASE TOHO 1; GLUTAMIC ACID; UNCLASSIFIED DRUG;

ACYLATION; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROGEN BOND; MUTANT; NEUTRON DIFFRACTION; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT;

ACYLATION; BETA-LACTAMASES; CATALYTIC DOMAIN; DEUTERIUM; GLUTAMIC ACID; HYDROGEN BONDING; MUTANT PROTEINS; MUTATION, MISSENSE; NEUTRON DIFFRACTION;

EID: 78651374505     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2010.12.017     Document Type: Article

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