Efficacy of selected purification techniques for bromelain

International Food Research Journal

Volumn 20, Issue 1, 2013, Pages 43-46

  Nadzirah, K Z ^a   Zainal, S ^a   Noriham, A ^a   Normah, I ^a  

^a UNIVERSITI TEKNOLOGI MARA   (Malaysia)

Author keywords

Ammonium sulphate; Aqueous two phase; Bromelain, reverse micellar systems; Chromatography; Extraction, cation exchange; Precipitation

Indexed keywords

ANANAS COMOSUS;

EID: 84871518583     PISSN: 19854668     EISSN: 22317546     Source Type: Journal    
DOI: None     Document Type: Short Survey

References (34)

1. Ali, I., Aboul-Enein, H.Y., Singh, P., Singh, R. and Sharma, B. 2010. Separation of biological proteins by liquid chromatography. Saudi Pharmaceutical Journal 18: 59-73.
2. Arakawa, T., Tsumoto, K., Ejima, D., Kita, Y., Yonezawa, Y. and Tokunaga, M. 2007. Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. Journal of Biochemical and Biophysical Methods 70: 493-498.
3. Babu, B.R., Rastogi, N.K. and Raghavarao, K.S.M.S. 2008. Liquid-liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system. Chemical Engineering and Processing 47: 83-89.
4. Barros, R.M., Ferreira, C.A., Silva, S.V. and Malcata, F.X. 2001. Quantitative studies on the enzymatic hydrolysis of milk proteins brought about by cardosins precipitated by ammonium sulfate. Enzyme and Microbial Technology 29: 541-547.
5. Bassani, G., Farruggia, B., Nerli, B., Romanini, B. and Pic?, G. 2007. Porcine pancreatic lipase partition in potassium phosphate-polyethylene glycol two phase systems. Journal of Chromatography B 859: 222-228.
6. Bhattacharyya, B.K. 2008. Bromelain: An overview. Natural Product Radiance 7(4): 359-363.
7. Brovko, F.A. and Zagranichnaya, T.K. 1998. Separation of proteins from phenols in cereal leaf extract by hydrophobic interaction-ammonium sulfate fractionation. Plant Physiology and Biochemistry 36 (10): 773-777.
8. Chobotova, K., Vernallis, A.B. and Abdul Majid, F.A. 2009. Bromelain's activity and potential as an anti-cancer agent: Current evidence and perspectives. Cancer Letters 20: 1-9.
9. de Oliveira, M.C., de Abreu Filho, M.A.N. and de Alĉantara Pesŝoa Filho, P. 2007. Phase equilibrium and protein partitioning in aqueous two-phase systems containing ammonium carbamate and block copolymers PEO-PPO-PEO. Biochemical Engineering Journal 37: 311-318.
10. Devakate, R.V., Patil, V.V., Waje, S.S. and Thorat, B.N. 2009. Purification and drying of bromelain. Separation and Purification Technology 64: 259-264.
11. Fernández, G. and Pomilio, A.B. 2003. Optimized growth conditions and determination of the catalytic type of the peptidase complex from a novel callus culture of pineapple (Ananas comosus). Molecular Medicinal Chemistry 1: 39-49.
12. Gautam, S. and Simon, L. 2006. Partitioning of β-glucosidase from Trichoderma reesei in poly (ethylene glycol) and potassium phosphate aqueous two-phase system: Influence of pH and temperature. Biochemical Engineering Journal 30: 104-108.
13. Hale, L.P., Fitzhugh, D.J. and Staats, H.F. 2006. Oral immunogenicity of the plant proteinase bromelain. International Immunopharmacology 6: 2038-2046.
14. Hale, L.P., Greer, P.K., Trinh, C.T. and James. C.L. 2005. Proteinase activity and stability of natural bromelain preparations. International Immunopharmacology 5: 783-793.
15. Harris, E.L.V. 1989. Concentration of the extract. In: Harris, E.L.V and Angal, S. (Eds.), Protein Purification Methods: A Practical Approach. Oxford University Press, New York, pp. 125-174.
16. Hebbar, H.U., Sumana, B. and Raghavarao, K.S.M.S. 2008. Use of reverse micellar systems for the extraction and purification of bromelain from pineapple waste. Bioresourse Technology 99: 4896-4902.
17. Li, M., Kim, J.W. and Peeples, T.L. 2002. Amylase partitioning and extractive bioconversion of starch using thermoseparating aqueous two-phase systems. Journal of Biotechnology 93: 15-26.
18. Lopez, L.M.I., Sequeiros, C., Natalucci, C.L., Brullo, A., Maras, B., Barra, D. and Caffini, N.O. 2000. Purification and characterization of macrodoctain l, a cysteine peptidase from unripe fruits of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae). Protein Expression and Purification 18: 133-140.
19. Madhusudhan, M.C., Raghavarao, K.S.M.S. and Nene, S. 2008. Integrated process for extraction and purification from Baker's yeast involving precipitation and aqueous two phase extraction. Biochemical Engineering Journal 38: 414-420.
20. Melendo, J.A., Beltrán, J.A., Jaime, I., Sancho, R. and Roncalés, P. 1996, Limited proteolysis of myofibrillar proteins by bromelain decreases toughness of coarse dry sausage. Food Chemistry 57: 429-433.
21. Ng, P.K., He, J. and Synder, M.K. 2009. Separation of proteins mixtures using PH-gradient cation exchange chromatography. Journal of Chromatography A 1216: 1372-1376.
22. Nishiki, T., Nakamura, K. and Kato, D. 2000. Forward and backward extraction rates of amino acid in reversed micellar extraction. Biochemical Engineering Journal 4: 189-195.
23. Porto, T.S., Medeiros e Silva, G.M., Porto, C.S., Cavalcanti, M.T.H., Neto, B.B., Lima-Filho, J.L., Converti, A., Porto, A.L.F. and Pessoa, Jr. A. 2008. Liquid-liquid extraction of proteases from fermented broth by PEG/ citrate aqueous two-phase system. Chemical Engineering and Processing 47: 716-721.
24. Rabelo, A.P.B., Tambourgi, E.B. and Pessoa, Jr. A. 2004. Bromelain partitioning in two-phase aqueous systems containing PEO-PPO-PEO block copolymers. Journal of Chromatography B 807: 61-68.
25. Raweeritha, R. and Ratanabanangkoon, K. 2003. Fractionation of equine antivenom using caprylic acid precipitation in combination with cationic ion-exchange chromatography. Journal of Immunological Methods 282: 63-72.
26. Rohrbach, K.G., Leal, F. and d'Eeckenbrugge, G.C. 2003. History, distribution and world production. In: Bartholomew, D.P., Paull, R.E. and Rohrbach, K.G. (Eds.), The Pineapple: Botany, Production and Uses. United Kingdom, pp. 1-12.
27. Rowan, A.D., Buttle, D.J. and Barrett, A.J. 1990. The cysteine proteinases of the pineapple plant. Biochemical Journal 266: 869-875.
28. Savelli, G., Spretib, N. and Profioa, P.D. 2000. Enzyme activity and stability control by amphiphilic self-organizing systems in aqueous solutions. Current Opinion in Colloid and Interface Science 5: 111-117.
29. Saxena, L., Iyer, B.K. and Ananthanarayan, L. 2007. Three phase partitioning as a novel method for purification of ragi (Eleusine coracana) bifunctional amylase/protease inhibitor. Process Biochemistry 42: 491-495.
30. Shin, H.G., Choi, Y.M., Kim, H.K., Ryu, Y.C., Lee, S.H. and Kim, B.C. 2008. Tenderisation and fragmentation of myofibrillar proteins in bovine longissimus dorsi muscle using proteolytic extract from Sarcodon aspratus. Lebensmittel-Wissenschaft und-Technologie 41: 1389-1395.
31. Singh, L.R., Devi, Y.R. and Devi, S.K. 2003. Enzymological characterization of pineapple extract for potential application in oak tasar (Antheraea proylei J.) silk cocoon cooking and reeling. Electronic Journal of Biotechnology 3: 199-207.
32. Soni, K. and Madamwar, D. 2000. Reversed micellar extraction of an extracellular acid phosphatase from fermentation broth. Process Biochemistry 36: 311-315.
33. Swadesh, J. 2001. Ion exchange chromatography. In: Swadesh, J. (Eds.), HPLC Practical and Industrial Applications. CRC Press, New York, pp. 213-285.
34. Vallés, D., Furtado, S. and Cantera, A.M.B. 2007. Characterization of news proteolytic enzymes from ripe fruits of Bromelia antiacantha Bertol. (Bromeliaceae). Enzyme and Microbial Technology 40: 409-413.