Separation of protein mixtures using pH-gradient cation-exchange chromatography

Journal of Chromatography A

Volumn 1216, Issue 9, 2009, Pages 1372-1376

  Ng, Paul K ^a   He, Jie ^a   Snyder, Mark A ^a  

^a BIO RAD LABORATORIES   (United States)

Author keywords

Cation exchange; Monoclonal antibody; Pegylated proteins; pH gradient

Indexed keywords

ACIDIC PROTEINS; BUFFER SYSTEMS; CATION-EXCHANGE; CATION-EXCHANGE CHROMATOGRAPHIES; CONJUGATED PROTEINS; CORRELATION COEFFICIENTS; ISO-ELECTRIC POINTS; LARGE PARTICLES; MONOCLONAL ANTIBODY; NATIVE PROTEINS; PEGYLATED PROTEINS; PH GRADIENT; PH-VALUES; PROTEIN MIXTURES; PROTEIN SPECIES; STEP CHANGES;

ADSORPTION; CHROMATOGRAPHIC ANALYSIS; ETHYLENE GLYCOL; GLYCOLS; ION EXCHANGE; IONIC STRENGTH; MONOCLONAL ANTIBODIES; PH; PH EFFECTS; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; POSITIVE IONS; PROTEINS; SEPARATION; SURFACE PLASMON RESONANCE;

ANTIBODIES;

ACID PROTEIN; BUFFER; CARBOXYL GROUP; MACROGOL; MONOCLONAL ANTIBODY; PROTEIN; PROTEIN A;

ACCURACY; ADSORPTION; ADSORPTION CHROMATOGRAPHY; ARTICLE; COLUMN CHROMATOGRAPHY; CONJUGATION; CONTROLLED STUDY; CORRELATION COEFFICIENT; ELUTION; ION EXCHANGE CHROMATOGRAPHY; IONIC STRENGTH; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; PARTICLE SIZE; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SEPARATION TECHNIQUE; TIME;

ANIMALS; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, ION EXCHANGE; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; ISOELECTRIC POINT; POLYETHYLENE GLYCOLS; PROTEINS; STAPHYLOCOCCAL PROTEIN A; SUPEROXIDE DISMUTASE;

EID: 59349083411     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2008.12.100     Document Type: Article

References (19)

1. Slutyterman L.A.A.E., and Elgersma O. J. Chromatogr. 150 (1978) 17
2. Gagnon P. Purification Tools for Monoclonal Antibodies (1996), Validated BioSystems, Tucson, AZ 57
3. Shan L., and Anderson D. J. Chromatogr. A 909 (2001) 191
4. Kang X., and Frey D. J. Chromatogr. A 991 (2003) 117
5. G.E. Life Sciences. Chromatofocusing with Polybuffer Exchangers, Instructions 52-1586-00 AL (2006), GE Healthcare Bio-Sciences, Uppsala
6. Tsonev L.I., and Hirsh A.G. J. Chromatogr. A 1200 (2008) 166
7. Bates R.C., and Frey D.D. J. Chromatogr. A 814 (1998) 43
8. Bates R.C., Kang X.K., and Frey D.D. J. Chromatogr. A 890 (2000) 25
9. Kang X.K., Bates R.C., and Frey D.D. J. Chromatogr. A 890 (2000) 37
10. Mhatre R., Nashabeh W., Schmalzing D., Yao X., Fuchs M., Whitney D., and Regnier F. J. Chromatogr. A 707 (1995) 225
11. Pabst T.M., Antos D., Carta G., Ramasubramanyan N., and Hunter A.K. J. Chromatogr. A 1181 (2007) 83
12. Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., and Bairoch A. In: Walker J.M. (Ed). The Proteomics Protocols Handbook (2005), Humana Press, New York 571
13. Ahamed T., Nfor B.K., Verhaert P.D.E.M., van Dedem G.W.K., van der Wielen L.A.M., Eppink M.H.M., van de Sandt E.J.A.X., and Ottens M. J. Chromatogr. A 1164 (2007) 181
14. Zhu K., Zhao J., Lubman D., Miller f.r., and Barder T.J. Ana. Chem. 77 (2005) 2745
15. Slutyterman L.A.A.E., and Wijdenes J. J. Chromatogr. 150 (1978) 31
16. Stein A., and Kiesewetter A. J. Chromatogr.B 848 (2007) 151
17. Ng P. In: Rathore A., and Velayudhan A. (Eds). Scale-up and Optimization in Preparative Chromatography (2003), Marcel Dekker, New York 273
18. Hall T., Wood D.C., and Smith S.E. J. Chromatogr. A 1041 (2004) 87
19. Seely J., and Richey C. J. Chromatogr. A 908 (2001) 235