Integrated process for extraction and purification of alcohol dehydrogenase from Baker's yeast involving precipitation and aqueous two phase extraction

Biochemical Engineering Journal

Volumn 38, Issue 3, 2008, Pages 414-420

  Madhusudhan, M C ^a   Raghavarao, K S M S ^a   Nene, Sanjay ^b  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

^b NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Alcohol dehydrogenase; Aqueous two phase extraction; Enzyme extraction; Precipitation; Purification; Yeast

Indexed keywords

AMMONIUM COMPOUNDS; EXTRACTION; POLYETHYLENE GLYCOLS; PRECIPITATION (CHEMICAL); PURIFICATION; YEAST;

ALCOHOL DEHYDROGENASE; ENZYME EXTRACTION; TWO PHASE EXTRACTION;

ENZYME ACTIVITY;

ALCOHOL DEHYDROGENASE;

ACID PRECIPITATION; AQUEOUS SOLUTION; AQUEOUS TWO PHASE EXTRACTION; ARTICLE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; EXTRACTION; PHASE SEPARATION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEPARATION TECHNIQUE; SUPERNATANT;

SACCHAROMYCES CEREVISIAE;

EID: 38849155190     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2007.08.007     Document Type: Article

References (27)

1. John H., and Eckfeldt R. Todd Light, kinetic ethylene glycol assay with the help of yeast alcohol dehydrogenase. Clin. Chem. 26 9 (1980) 1278-1280
2. Gonchar M., Maiden M., Korpan Y., Sibirny V., Kotylak Z., and Sibirny A. Metabolically engineered methylotrophic yeast cells and enzymes as sensor recognition elements. FEMS Yeast Res. 2 (2002) 307-314
3. Willoughby N.A., Kirschner T., Smith M.P., Hjorth R., and Titcher-Hooker N.J. Immobilised metal ion affinity chromatography purification of alcohol dehydrogenase from Baker's yeast using an expanded bed adsorption system. J. Chromatogr. A 840 (1999) 195-204
4. Hidayat C., Takagi M., and Yoshida T. Expanded bed adsorption for purification of alcohol dehydrogenase using a dye-iminodiacetic acid matrix. J. Biosci. Bioeng. 97 4 (2004) 284-287
5. Negoro M., and Wakabayashi I. New simple method for purification of class I alcohol dehydrogenase. Alcohol Alcohol. 39 (2004) 178-182
6. Mahadevan H., and Hall C.K. Theory of precipitation of protein mixtures by nonionic polymer. AIChE J. 38 4 (1992) 573-591
7. Atha D.H., and Inghamģ K.C. Mechanism of precipitation of proteins by poly ethylene glycol. J. Biol. Chem. 256 23 (1981) 12108-12117
8. Albertsson P.A. Partition of Cell Particles and Molecules (1985), Wiley, New York
9. Raghavarao K.S.M.S., Rastogi N.K., Gowthaman M.K., and Karanth N.G. Aqueous two phase extraction for downstream processing of enzymes/proteins. Adv. Microbiol. 41 (1995) 97-171
10. Tanuja S., Srinivas N.D., Raghavarao K.S.M.S., and Gowthaman M.K. Aqueous two phase extraction for down stream processing of amyloglucosidase. Process Biochem. 32 8 (1997) 635-641
11. Zhi-Guo-Su, and Feng X.-L. Process integration of cell disruption and aqueous two phase extractions. J. Chem. Technol. Biotechnol. 74 (1999) 284-288
12. Hustedt H., Kroner K.H., Menge U., and Kula M.R. Protein recovery using two-aqueous systems. Trends Biotechnol. 3 6 (1985) 139-144
13. Zaslavasky B.Y. Aqueous Two Phase Partitioning: Physical Chemistry and Bioanalytical Applications (1995), Marcel Decker, New York
14. Bradford M.A. Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principles of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
15. Kagi J.H.R., and Vallee B.L. The role of zinc in alcohol dehydrogenase-V. The effect of metal-binding agents on the structure of the yeast alcohol dehydrogenase molecule. J. Biol. Chem. 235 (1960) 3188-3192
16. Bergmeyer H.U. Enzymes as biochemical reagents. In: Bergmeyer H.U. (Ed). Methods of Enzymatic Analysis vol. 1 (1974), Academic Press, Inc., New York 428-429
17. Deuscher M. Methods in enzymology guide to protein purification; purification procedures. Electrophoret. Meth. 182 (1990) 425-488
18. Zanon J.P., Peres M.F.S., and Gattas E.A.L. Colorimetric assay of ethanol using alcohol dehydrogenase from dry Baker's yeast. Enzym. Micro. Technol. 40 (2007) 466-470
19. Almedia M.C., Venancio A., Teixeira J.A., and Aires-Barros M.R. Cutinase purification on poly (ethylene-glycol-hydroxy propyl) starch aqueous two phase systems. J. Chromatogr. B 711 (1998) 151-159
20. Rabelo A.P.B., Tombourgi E.B., and Pressoa Jr. A. Bromelain partitioning in two phase aqueous systems containing PEO-PPO-PEO block copolymers. J. Chromatogr. B 807 (2004) 61-68
21. Smith M.P., Bulmer M.A., Hjorth R., and Titchener-Hooker N.J. Hydrophobic interaction ligand selection and scale up of an expanded bed separation of an intracellular enzyme from Saccharomyces cerevisiae. J. Chromatogr. A 968 (2002) 121-128
22. Foster P.R., Dunnill P., and Lilly M.D. Salting out of enzymes with ammonium sulphate. Biotechnol. Bioeng. 13 (1971) 713
23. Rito-Palomares M. Practical application of aqueous two phase partition to process development for the recovery of biological products. J. Chromatogr. B 807 (2005) 3-11
24. Rito-Palomares M., and Lyddiatt A. Practical implementation of aqueous two phase processes for protein recovery from yeast. J. Chem. Technol. Biotechnol. 75 (2000) 632-638
25. Zhang T.-X., Liu H.-Z., and Chen J.-Y. Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB. J. Chem. Technol. Biotechnol. 75 (2000) 798-802
26. Chang Y.-K., Chen J.-P., Sheu J.-R., Cheng P.-J., Su C.-H., and Chou S.-Y. Direct recovery of alcohol dehydrogenase from unclarified yeast cell homogenate by IDEBAC using an improved scheme for elution. Biochem. Eng. J. 30 (2006) 1-10
27. Muthalik R.B., and Gaikar V.G. Reverse micellar solutions aided permealization of Baker's yeast. Process Biochem. 4 (2006) 133-141