Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Vermeire, Jolien ^a   Naessens, Evelien ^a   Vanderstraeten, Hanne ^a   Landi, Alessia ^a   Iannucci, Veronica ^a   van Nuffel, Anouk ^a   Taghon, Tom ^a   Pizzato, Massimo ^b   Verhasselt, Bruno ^a  

^a GHENT UNIVERSITY   (Belgium)

^b UNIVERSITY OF TRENTO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN P24; LENTIVIRUS VECTOR; RETROVIRUS VECTOR; RNA DIRECTED DNA POLYMERASE;

ACCURACY; ARTICLE; ASSAY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; INTERMETHOD COMPARISON; MOLONEY LEUKEMIA ONCOVIRUS; MS2 VIRUS; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SUPERNATANT; SYBR GREEN I BASED REAL TIME PRODUCT ENHANCED REVERSE TRANSCRIPTASE ASSAY; VIRUS TITRATION;

CELL LINE; GENETIC VECTORS; HIV REVERSE TRANSCRIPTASE; HIV-1; HUMANS; ORGANIC CHEMICALS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TITRIMETRY;

HUMAN IMMUNODEFICIENCY VIRUS 1; MOLONEY MURINE LEUKEMIA VIRUS;

EID: 84870794405     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0050859     Document Type: Article

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