A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants

Journal of Virological Methods

Volumn 156, Issue 1-2, 2009, Pages 1-7

  Pizzato, Massimo ^a   Erlwein, Otto ^a   Bonsall, David ^a   Kaye, Stephen ^a   Muir, David ^b   McClure, Myra O ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b IMPERIAL COLLEGE HEALTHCARE NHS TRUST   (United Kingdom)

Author keywords

HIV; Real time PCR; Retrovirus; Reverse transcriptase assay

Indexed keywords

FLUORESCENT DYE;

ARTICLE; CONTROLLED STUDY; CULTURE TECHNIQUE; ENZYME ASSAY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MURINE LEUKEMIA VIRUS; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RETROVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPUMA VIRUS; VIRUS PARTICLE;

CELL CULTURE TECHNIQUES; CELL LINE; FLUORESCENT DYES; HIV-1; HUMANS; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; RETROVIRIDAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SPUMAVIRUS;

HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN SPUMARETROVIRUS; MURINE LEUKEMIA VIRUS;

EID: 59749086857     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.10.012     Document Type: Article

References (35)

1. Adachi A., Gendelman H.E., Koenig S., Folks T., Willey R., Rabson A., and Martin M.A. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J. Virol. 59 (1986) 284-291
2. Arezi B., Xing W., Sorge J.A., and Hogrefe H.H. Amplification efficiency of thermostable DNA polymerases. Anal. Biochem. 321 (2003) 226-235
3. Arnold B.A., Hepler R.W., and Keller P.M. One-step fluorescent probe product-enhanced reverse transcriptase assay. Biotechniques 25 (1998) 98-106
4. Bisset L.R., Boni J., Lutz H., and Schupbach J. Lack of evidence for PERV expression after apoptosis-mediated horizontal gene transfer between porcine and human cells. Xenotransplantation 14 (2007) 13-24
5. Brorson K., Swann P.G., Lizzio E., Maudru T., Peden K., and Stein K.E. Use of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus levels in mAb cell-culture and downstream processing. Biotechnol. Prog. 17 (2001) 188-196
6. Brorson K., Xu Y., Swann P.G., Hamilton E., Mustafa M., de Wit C., Norling L.A., and Stein K.E. Evaluation of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus in mAb cell-culture. Biologicals 30 (2002) 15-26
7. Chang A., Ostrove J.M., and Bird R.E. Development of an improved product enhanced reverse transcriptase assay. J. Virol. Methods 65 (1997) 45-54
8. Dimitrov D.S., Willey R.L., Sato H., Chang L.J., Blumenthal R., and Martin M.A. Quantitation of human immunodeficiency virus type 1 infection kinetics. J. Virol. 67 (1993) 2182-2190
9. Fan X.Y., Lu G.Z., Wu L.N., Chen J.H., Xu W.Q., Zhao C.N., and Guo S.Q. A modified single-tube one-step product-enhanced reverse transcriptase (mSTOS-PERT) assay with heparin as DNA polymerase inhibitor for specific detection of RTase activity. J. Clin. Virol. 37 (2006) 305-312
10. Garcia Lerma J.G., Yamamoto S., Gomez-Cano M., Soriano V., Green T.A., Busch M.P., Folks T.M., and Heneine W. Measurement of human immunodeficiency virus type 1 plasma virus load based on reverse transcriptase (RT) activity: evidence of variabilities in levels of virion-associated RT. J. Infect. Dis. 177 (1998) 1221-1229
11. Heinkelein M., Rammling M., Juretzek T., Lindemann D., and Rethwilm A. Retrotransposition and cell-to-cell transfer of foamy viruses. J. Virol. 77 (2003) 11855-11858
12. Heneine W., Yamamoto S., Switzer W.M., Spira T.J., and Folks T.M. Detection of reverse transcriptase by a highly sensitive assay in sera from persons infected with human immunodeficiency virus type 1. J. Infect. Dis. 171 (1995) 1210-1216
13. Karsai A., Muller S., Platz S., and Hauser M.T. Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expression. Biotechniques 32 790-792 (2002) 794-796
14. Khan A.S., and Sears J.F. Pert analysis of endogenous retroviruses induced from K-BALB mouse cells treated with 5-iododeoxyuridine: a potential strategy for detection of inducible retroviruses from vaccine cell substrates. Dev. Biol. (Basel) 106 387-392 (2001) 392-393
15. Kimpton J., and Emerman M. Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene. J. Virol. 66 (1992) 2232-2239
16. Lekanne Deprez R.H., Fijnvandraat A.C., Ruijter J.M., and Moorman A.F. Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Anal. Biochem. 307 (2002) 63-69
17. Lovatt A., Black J., Galbraith D., Doherty I., Moran M.W., Shepherd A.J., Griffen A., Bailey A., Wilson N., and Smith K.T. High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods. J. Virol. Methods 82 (1999) 185-200
18. Maudru T., and Peden K. Elimination of background signals in a modified polymerase chain reaction-based reverse transcriptase assay. J. Virol. Methods 66 (1997) 247-261
19. Maudru T., and Peden K.W. Adaptation of the fluorogenic 5′-nuclease chemistry to a PCR-based reverse transcriptase assay. Biotechniques 25 (1998) 972-975
20. Naldini L., Blomer U., Gallay P., Ory D., Mulligan R., Gage F.H., Verma I.M., and Trono D. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector [see comments]. Science 272 (1996) 263-267
21. Neil S., Martin F., Ikeda Y., and Collins M. Postentry restriction to human immunodeficiency virus-based vector transduction in human monocytes. J. Virol. 75 (2001) 5448-5456
22. Pastorino B., Bessaud M., Grandadam M., Murri S., Tolou H.J., and Peyrefitte C.N. Development of a TaqMan RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses. J. Virol. Methods 124 (2005) 65-71
23. Pierce K.E., Rice J.E., Sanchez J.A., and Wangh L.J. QuantiLyse: reliable DNA amplification from single cells. Biotechniques 32 (2002) 1106-1111
24. Pietschmann T., Heinkelein M., Heldmann M., Zentgraf H., Rethwilm A., and Lindemann D. Foamy virus capsids require the cognate envelope protein for particle export. J. Virol. 73 (1999) 2613-2621
25. Pizzato M., Blair E.D., Fling M., Kopf J., Tomassetti A., Weiss R.A., and Takeuchi Y. Evidence for nonspecific adsorption of targeted retrovirus vector particles to cells. Gene Ther. 8 (2001) 1088-1096
26. Pyra H., Boni J., and Schupbach J. Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement. Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 1544-1548
27. Sastry L., Xu Y., Duffy L., Koop S., Jasti A., Roehl H., Jolly D., and Cornetta K. Product-enhanced reverse transcriptase assay for replication-competent retrovirus and lentivirus detection. Hum. Gene Ther. 16 (2005) 1227-1236
28. Sears J.F., and Khan A.S. Single-tube fluorescent product-enhanced reverse transcriptase assay with Ampliwax (STF-PERT) for retrovirus quantitation. J. Virol. Methods 108 (2003) 139-142
29. Silver J., Maudru T., Fujita K., and Repaske R. An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions. Nucleic Acids Res. 21 (1993) 3593-3594
30. Soneoka Y., Cannon P.M., Ramsdale E.E., Griffiths J.C., Romano G., Kingsman S.M., and Kingsman A.J. A transient three-plasmid expression system for the production of high titer retroviral vectors. Nucleic Acids Res. 23 (1995) 628-633
31. Takeuchi, Y., McClure, M.O., Pizzato, M, 2008. Identification of gamma-retroviruses constitutively released from cell lines used for HIV research. J. Virol. 82 (24).
32. Teo I.A., Choi J.W., Morlese J., Taylor G., and Shaunak S. LightCycler qPCR optimisation for low copy number target DNA. J. Immunol. Methods 270 (2002) 119-133
33. Varga A., and James D. Detection and differentiation of Plum pox virus using real-time multiplex PCR with SYBR Green and melting curve analysis: a rapid method for strain typing. J. Virol. Methods 123 (2005) 213-220
34. Voisset C., Tonjes R.R., Breyton P., Mandrand B., and Paranhos-Baccala G. Specific detection of RT activity in culture supernantants of retrovirus-producing cells, using synthetic DNA as competitor in polymerase enhanced reverse transcriptase assay. J. Virol. Methods 94 (2001) 187-193
35. Yamamoto S., Folks T.M., and Heneine W. Highly sensitive qualitative and quantitative detection of reverse transcriptase activity: optimization, validation, and comparative analysis with other detection systems. J. Virol. Methods 61 (1996) 135-143