High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods

Journal of Virological Methods

Volumn 82, Issue 2, 1999, Pages 185-200

  Lovatt, A ^a   Black, J ^a   Galbraith, D ^a   Doherty, I ^a   Moran, M W ^a   Shepherd, A J ^a   Griffen, A ^a   Bailey, A ^a   Wilson, N ^a   Smith, K T ^a  

^a Q One Biotech Ltd   (United Kingdom)

Author keywords

Calf thymus DNA; DNA polymerase; PBRT; Product enhanced reverse transcriptase assay; Retrovirus; TaqMan PCR; Xenotransplantation

Indexed keywords

RNA DIRECTED DNA POLYMERASE;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE REPRESSION; HUMAN; HUMAN CELL; MURINE LEUKEMIA VIRUS; NONHUMAN; PRIORITY JOURNAL; RETROVIRUS; REVERSE TRANSCRIPTION; SIMIAN IMMUNODEFICIENCY VIRUS; SIMIAN VIRUS; VIRUS DETECTION;

ANIMALS; CATALYSIS; CATIONS, DIVALENT; CELL LINE; CULTURE MEDIA, CONDITIONED; DNA; DNA-DIRECTED DNA POLYMERASE; FALSE POSITIVE REACTIONS; FLUORESCENT DYES; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; RETROVIRIDAE; RNA-DIRECTED DNA POLYMERASE; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC; VIRAL PROTEINS;

EID: 0032731390     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00111-1     Document Type: Article

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