Evaluation of different RT enzyme standards for quantitation of retroviruses using the single-tube fluorescent product-enhanced reverse transcriptase assay

Journal of Virological Methods

Volumn 157, Issue 2, 2009, Pages 133-140

  Ma, Yun Kun ^a   Khan, Arifa S ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

Retroviruses; Reverse transcriptase; STF PERT assay

Indexed keywords

ENZYME;

ARTICLE; AVIAN MYELOBLASTOSIS VIRUS; CLINICAL EFFECTIVENESS; EVALUATION; HUMAN IMMUNODEFICIENCY VIRUS 1; MURINE LEUKEMIA VIRUS; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; RETROVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SINGLE TUBE FLUORESCENT PRODUCT ENHANCED REVERSE TRANSCRIPTASE ASSAY; VIRUS DETECTION;

HUMANS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; RETROVIRIDAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA-DIRECTED DNA POLYMERASE; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

AVIAN MYELOBLASTOSIS VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; MURINE LEUKEMIA VIRUS;

EID: 63449142595     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.01.002     Document Type: Article

References (21)

1. Arnold B.A., Hepler R.W., and Keller P.M. One-step fluorescent probe product-enhanced reverse transcriptase assay. Biotechniques 25 (1998) 98-106
2. Böni J., Stalder J., Reigel F., and Schüpbach J. Detection of reverse transcriptase activity in live attenuated virus vaccines. Clin. Diagn. Virol. 5 (1996) 43-53
3. Brorson K., Swann P.G., Lizzio E., Maudru T., Peden K., and Stein K.E. Use of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus levels in mAb cell-culture and downstream processing. Biotechnol. Prog. 17 (2001) 188-196
4. Clemenceau B., Jegou D., Martignat L., and Sai P. Long-term follow-up failed to detect in vitro transmission of full-length porcine endogenous retroviruses from specific pathogen-free pig islets to human cells. Diabetologia 44 (2001) 2044-2055
5. Garcia Lerma J., Schinazi R.F., Juodawlkis A.S., Soriano V., Lin Y., Tatti K., Rimland D., Folks T.M., and Heneine W. A rapid non-culture-based assay for clinical monitoring of phenotypic resistance of human immunodeficiency virus type 1 to lamivudine (3TC). Antimicrob. Agents Chemother. 43 (1999) 264-270
6. Heneine W., Switzer W.M., Soucie J.M., Evatt B.L., Shanmugam V., Rosales G.V., Matthews A., Sandstrom P., and Folks T.M. Evidence of porcine endogenous retroviruses in porcine factor VIII and evaluation of transmission to recipients with hemophilia. J. Infect. Dis. 183 (2001) 648-652
7. Heneine W., Yamamoto S., Switzer W.M., Spira T.J., and Folks T.M. Detection of reverse transcriptase by a highly sensitive assay in sera from persons infected with human immunodeficiency virus type 1. J. Infect. Dis. 171 (1995) 1210-1216
8. Hussain A.I., Johnson J.A., Da Silva Freire M., and Heneine W. Identification and characterization of avian retroviruses in chicken embryo-derived yellow fever vaccines: investigation of transmission to vaccine recipients. J. Virol. 77 (2003) 1105-1111
9. Johnson J.A., and Heneine W. Characterization of endogenous avian leukosis viruses in chicken embryonic fibroblast substrates used in production of measles and mumps vaccines. J. Virol. 75 (2001) 3605-3612
10. Khan A.S., Maudru T., Thompson A., Muller J., Sears J.F., and Peden K.W. The reverse transcriptase activity in cell-free medium of chicken embryo fibroblast cultures is not associated with a replication-competent retrovirus. J. Clin. Virol. 11 (1998) 7-18
11. Layne S.P., Merges M.J., Dembo M., Spouge J.L., Conley S.R., Moore J.P., Raina J.L., Renz H., Gelderblom H.R., and Nara P.L. Factors underlying spontaneous inactivation and susceptibility to neutralization of human immunodeficiency virus. Virology 189 (1992) 695-714
12. Lovatt A., Black J., Galbraith D., Doherty I., Moran M.W., Shepherd A.J., Griffen A., Bailey A., Wilson N., and Smith K.T. High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods. J. Virol. Meth. 82 (1999) 185-200
13. Maudru T., and Peden K.W. Adaptation of the fluorogenic 5′-nuclease chemistry to a PCR-based reverse transcriptase assay. Biotechniques 25 (1998) 972-975
14. Panet A., Baltimore D., and Hanafusa T. Quantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay. J. Virol. 16 (1975) 146-152
15. Pyra H., Böni J., and Schüpbach J. Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 1544-1548
16. Sastry L., Xu Y., Duffy L., Koop S., Jasti A., Roehl H., Jolly D., and Cornetta K. Product-enhanced reverse transcriptase assay for replication-competent retrovirus and lentivirus detection. Hum. Gene Ther. 16 (2005) 1227-1236
17. Sears J.F., and Khan A.S. Single-tube fluorescent product-enhanced reverse transcriptase assay with AmpliWax (STF-PERT) for retrovirus quantitation. J. Virol. Meth. 108 (2003) 139-142
18. 2+-based reverse transcriptase assay for detection of primate retroviruses. J. Clin. Microbiol. 37 (1999) 1704-1708
19. Shahabuddin M., Sears J.F., and Khan A.S. No evidence of infectious retroviruses in measles virus vaccines produced in chicken embryo cell cultures. J. Clin. Microbiol. 39 (2001) 675-684
20. Silver J., Maudru T., Fujita K., and Repaske R. An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions. Nucl. Acids Res. 21 (1993) 3593-3594
21. Weissmahr R.N., Schüpbach J., and Böni J. Reverse transcriptase activity in chicken embryo fibroblast culture supernatants is associated with particles containing endogenous avian retrovirus EAV-0 RNA. J. Virol. 71 (1997) 3005-3012