Comparison of lentiviral vector titration methods

BMC Biotechnology

Volumn 6, Issue , 2006, Pages

  Geraerts, Martine ^a   Willems, Sofie ^a   Baekelandt, Veerle ^b   Debyser, Zeger ^a   Gijsbers, Rik ^a  

^a DEPARTMENT OF PHARMACEUTICAL AND PHARMACOLOGICAL SCIENCES   (Belgium)

^b UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

LENTIVIRAL VECTORS; STABLE GENE TRANSFER; THERAPEUTIC GENE EXPRESSION; TITRATION METHODS; TRANSGENE EXPRESSION;

BIOCHEMISTRY; CELLS; PROTEINS; RNA; TITRATION;

GENE TRANSFER;

GENOMIC RNA; LENTIVIRUS VECTOR; MESSENGER RNA; VIRUS RNA;

ARTICLE; CELL DIVISION; CONTROLLED STUDY; CORRELATION ANALYSIS; GENE EXPRESSION; GENE TRANSFER; GENETIC CODE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; PREDICTION; QUALITY CONTROL; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SAFETY; TRANSGENE; VALIDATION PROCESS; VIRAL GENE DELIVERY SYSTEM; VIRUS TITRATION;

CHROMOSOME MAPPING; DNA, VIRAL; GENETIC VECTORS; GENOME, VIRAL; LENTIVIRUS; POLYMERASE CHAIN REACTION; RNA, VIRAL; TRANSFECTION;

EID: 33746978775     PISSN: 14726750     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-6-34     Document Type: Article

References (27)

1. Bukrinsky MI, Haggerty S, Dempsey MP, Sharova N, Adzhubel A, Spitz L, Lewis P, Goldfarb D, Emerman M, Stevenson M: A nuclear localization signal within HIV-I matrix protein that governs infection of non-dividing cells. Nature 1993, 365:666-669.
2. Naldini L: Lentiviruses as gene transfer agents for delivery to non-dividing cells. Curr Opin Biotechnol 1998, 9:457-463.
3. Miyoshi H, Smith KA, Mosier DE, Verma IM, Torbett BE: Transduction of human CD34+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors. Science 1999, 283:682-686.
4. Baekelandt V, Claeys A, Eggermont K, Lauwers E, De Strooper B, Nuttin B, Debyser Z: Characterization of lentiviral vector-mediated gene transfer in adult mouse brain. Human Gene Therapy 2002, 13:841-853.
5. Naldini L, Blömer U, Gage FH, Trono D, Verma IM: Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci U S A 1996, 93:11382-11388.
6. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D: In vivo gene delivery and stable transduction of non-dividing cells by a lentiviral vector. Science 1996, 272:263-267.
7. Debyser Z: Biosafety of lentiviral vectors. Curr Gene Ther 2003, 3:517-525.
8. Geraerts M, Michiels M, Baekelandt V, Debyser Z, Gijsbers R: Upscaling of lentiviral vector production by tangential flow filtration. J Gene Med 2005, 7:1299-1310.
9. Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, Naldini L, Trono D: Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. Journal of Virology 1998, 72:9873-9880.
10. Zufferey R, Donello JE, Trono D, Hope TJ: Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. Journal of Virology 1999, 73:2886-2892.
11. Manganini M, Serafini M, Bambacioni F, Casati C, Erba E, Follenzi A, Naldini L, Bemasconi S, Gaipa G, Rambaldi A, Biondi A, Golay J, Introna M: A Human Immunodeficiency Virus Type I pol Gene-Derived Sequence (cPPT/ CTS) Increases the Efficiency of Transduction of Human Nondividing Monocytes and T Lymphocytes by Lentiviral Vectors. Hum Gene Ther 2002, 13:1793-1807.
12. Delenda C, Gaillard C: Real-time quantitative PCR for the design of lentiviral vector analytical assays. Gene Ther 2005, 12:S36-50.
13. Sastry L, Johnson T, Hobson MJ, Smucker B, Cornetta K: Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods. Gene Ther 2002, 9:1155-1162.
14. Scherr M, Battmer K, Blomer U, Ganser A, Grez M: Quantitative determination of lentiviral vector particle numbers by realtime PCR. Biotechniques 2001, 31:520, 522, 524, passim.
15. Lizee G, Aerts JL, Gonzales MI, Chinnasamy N, Morgan RA, Topalian SL: Real-time quantitative reverse transcriptase-polymerase chain reaction as a method for determining lentiviral vector titers and measuring transgene expression. Hum Gene Ther 2003, 14:497-507.
16. Logan AC, Nightingale SJ, Haas DL, Cho GJ, Pepper KA, Kohn DB: Factors influencing the titer and infectivity of lentiviral vectors. Hum Gene Ther 2004, 15:976-988.
17. Zhang B, Metharom P, Jullie H, Ellem KA, Cleghorn G, West MJ, Wei MQ: The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events. Genet Vaccines Ther 2004, 2:6.
18. Van Maele B, De Rijck J, De Clercq E, Debyser Z: Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type I vector transduction. J Virol 2003, 77:4685-4694.
19. Farson D, Witt R, McGuinness R, Dull T, Kelly M, Song J, Radeke R, Bukovsky A, Consiglio A, Naldini L: A new generation stable inducible packaging cell line for lentiviral vectors. Hum Gene Ther 2001, 12:981-997.
20. Ikeda Y, Takeuchi Y, Martin F, Cosset FL, Mitrophanous K, Collins M: Continuous high-titer HIV-I vector production. Nat Biotechnol 2003, 21:569-572.
21. Kafri T, van Praag H, Ouyang L, Gage FH, Verma IM: A packaging cell line for lentivirus vectors. J Virol 1999, 73:576-584.
22. Follenzi A, Ailles LE, Bakovic S, Geuna M, Naldini L: Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-I pol sequences. Nature Genetics 2000, 25:217-222.
23. Zennou V, Serguera C, Sarkis C, Colin P, Perret E, Mallet J, Charneau P: The HIV-I DNA flap stimulates HIV vector-mediated cell transduction in the brain. Nature Biotechnology 2001, 19:446-450.
24. Ikeda Y, Collins MK, Radcliffe PA, Mitrophanous KA, Takeuchi Y: Gene transduction efficiency in cells of different species by HIV and EIAV vectors. Gene Therapy 2002, 9:932-938.
25. Kirkwood TB, Bangham CR: Cycles, chaos, and evolution in virus cultures: a model of defective interfering particles. Proc Natl Acad Sci U S A 1994, 91:8685-8689.
26. Butler SL, Hansen MS, Bushman FD: A quantitative assay for HIV DNA integration in vivo. Nat Med 2001, 7:631-634.
27. Martin-Rendon E, White LJ, Olsen A, Mitrophanous KA, Mazarakis ND: New Methods to Titrate EIAV-Based Lentiviral Vectors. Mol Ther 2002, 5:566-570.