Titering lentiviral vectors: Comparison of DNA, RNA and marker expression methods

Gene Therapy

Volumn 9, Issue 17, 2002, Pages 1155-1162

  Sastry, L ^a   Johnson, T ^a   Hobson, M J ^a   Smucker, B ^a   Cornetta, K ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA titer; GFP titer; Lentiviral vectors; Packaging probe; Real time PCR; RNA titer

Indexed keywords

AMPICILLIN; BETA GALACTOSIDASE; BIOLOGICAL MARKER; GENOMIC DNA; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; PLASMID DNA; VIRUS DNA; VIRUS RNA;

ARTICLE; CELL LINE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; EXPRESSION VECTOR; FLOW CYTOMETRY; GENETIC TRANSDUCTION; HELA CELL; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; INTERMETHOD COMPARISON; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RELIABILITY; REPRODUCIBILITY; RNA ANALYSIS; RNA SEQUENCE; SEQUENCE ANALYSIS; SUPERNATANT; TRANSGENE; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; CELL LINE; DNA, VIRAL; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LENTIVIRUS; LUMINESCENT PROTEINS; POLYMERASE CHAIN REACTION; RNA, VIRAL; TRANSDUCTION, GENETIC;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; DNA VIRUSES; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; LENTIVIRUS; MUS; MUS DUNNI; RNA VIRUSES; VECTORS;

EID: 0036713630     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301731     Document Type: Article

References (20)

1. Lentiviral vectors: Excellent tools for experimental gene transfer and promising candidates for gene therapy
2. Lentiviral vectors
3. Development of lentiviral vectors for gene therapy for human diseases
4. A rapid screening procedure for the identification of high-titer retrovirus packaging clones
5. Rapid titer determination using quantitative real-time PCR
6. Rapid retrovirus titration using competitive polymerase chain reaction
7. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells
8. Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells
9. A simple and reliable method for screening retroviral producer clones without selectable markers
10. Retroviral particles produced from a stable human-derived packaging cell line transduce target cells with very high efficiencies
11. Use of enhanced green fluorescent protein to optimize and quantitate infection of target cells with recombinant retroviruses
12. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector
13. Retrovirus packaging cells
14. Functional correction of fanconi anemia group C hematopoietic cells by the use of a novel lentiviral vector
15. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
16. A third-generation lentivirus vector with a conditional packaging system
17. Cycles, chaos, and evolution in virus cultures: A model of defective interfering particles
18. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection
19. Lentivirus vectors using human and simian immunodeficiency virus elements
20. Packaging cell line DNA contamination of vector supernatants: Implication for laboratory and clinical research