Transplantation of a hydrogen bonding network from West Nile virus protease onto Dengue-2 protease improves catalytic efficiency and sheds light on substrate specificity

Protein Engineering, Design and Selection

Volumn 25, Issue 12, 2012, Pages 843-850

  Doan, Danny N P ^a   Li, Kun Quan ^b   Basavannacharya, Chandrakala ^a   Vasudevan, Subhash G ^a   Madhusudhan, M S ^b,c  

^a DUKE NUS MEDICAL SCHOOL   (Singapore)

^b BIOINFORMATICS INSTITUTE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Dengue virus protease; homology modelling; hydrogen bonding network; protein engineering; West Nile virus protease

Indexed keywords

DENGUE VIRUS; HOMOLOGY MODELLING; HYDROGEN BONDING NETWORK; PROTEIN ENGINEERING; WEST NILE VIRUS;

ALDEHYDES; AMINO ACIDS; BINDING ENERGY; GENETIC ENGINEERING; HYDROGEN BONDS; PEPTIDES;

VIRUSES;

APROTININ; GLUTAMIC ACID; MUTANT PROTEIN; SERINE PROTEINASE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; COVALENT BOND; DENGUE VIRUS 2; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; HYDROGEN BOND; IC 50; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN STRUCTURE; SEROTYPE; WEST NILE FLAVIVIRUS;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; DENGUE VIRUS; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; PROTEASE INHIBITORS; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS; WEST NILE VIRUS;

DENGUE VIRUS; FLAVIVIRUS; WEST NILE VIRUS;

EID: 84870206891     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs049     Document Type: Article

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