Identification of the limitations on recombinant gene expression in CHO cell lines with varying luciferase production rates

Biotechnology and Bioengineering

Volumn 102, Issue 6, 2009, Pages 1593-1602

  Mead, Emma J ^a   Chiverton, Lesley M ^a   Smales, C Mark ^a   Von Haar, Tobias Der ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

Author keywords

Mammalian cell culture; Posttranscriptional control; Protein turnover; Recombinant protein; Translational control

Indexed keywords

CELL LINES; CHO CELLS; EMPIRICAL APPROACHES; MAMMALIAN CELL CULTURE; MAMMALIAN CELL LINES; POSTTRANSCRIPTIONAL CONTROL; PRODUCTION RATES; RECOMBINANT GENE EXPRESSIONS; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; TRANSCRIPTIONAL LEVELS; TRANSLATIONAL CONTROL; UPPER LIMITS; VARYING PRODUCTIVITIES;

ANIMAL CELL CULTURE; BIOACTIVITY; CELL MEMBRANES; GENE ENCODING; MAMMALS; NUCLEIC ACIDS; PROTEIN FOLDING; STRATEGIC PLANNING; TRANSCRIPTION;

CELL CULTURE;

BETA ACTIN; DNA; LUCIFERASE; MESSENGER RNA; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL GROWTH; CHO CELL; GENE EXPRESSION; GENETIC TRANSCRIPTION; GROWTH RATE; HALF LIFE TIME; IMMUNOCYTOCHEMISTRY; LUMINESCENCE; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN PROCESSING; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; WESTERN BLOTTING;

ANALYSIS OF VARIANCE; ANIMALS; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION; LUCIFERASES; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

MAMMALIA;

EID: 65349129412     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22201     Document Type: Article

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