Generation of monoclonal antibody fragments binding the native γ-secretase complex for use in structural studies

Biochemistry

Volumn 51, Issue 44, 2012, Pages 8779-8790

  Alattia, Jean René ^a   Schweizer, Claude ^a   Cacquevel, Matthias ^a   Dimitrov, Mitko ^a   Aeschbach, Lorène ^a   Oulad Abdelghani, Mustapha ^b   Fraering, Patrick C ^a  

^a EPFL   (Switzerland)

^b INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALZHEIMER'S DISEASE; CATALYTIC PROPERTIES; CHO CELL; CRYSTAL FORMATION; DOSE-DEPENDENT MANNER; ENZYME COMPLEXES; FAB FRAGMENTS; HIGH-RESOLUTION STRUCTURES; MEMBRANE PROTEINS; NANOLITERS; NICASTRIN; SECRETASE COMPLEX; SECRETASES; SPECIFIC TOOL; STRUCTURAL STUDIES; THERAPEUTIC COMPOUNDS;

BIOLOGICAL MEMBRANES; CELL CULTURE; PURIFICATION;

MONOCLONAL ANTIBODIES;

ANTERIOR PHARYNX DEFECTIVE 1 PROTEIN ANTIBODY; ENZYME ANTIBODY; EPITOPE; GAMMA SECRETASE; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 2G6; MONOCLONAL ANTIBODY 2H10; NICASTRIN ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY ISOLATION; ANTIBODY PRODUCTION; ANTIGEN ANTIBODY COMPLEX; ANTIGEN BINDING; ARTICLE; CATALYSIS; CHO CELL; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTALLIZATION; ENZYME ISOLATION; EPITOPE MAPPING; FEMALE; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IMMUNIZATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

AMYLOID PRECURSOR PROTEIN SECRETASES; ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHOLIC ACIDS; CRICETINAE; CRICETULUS; CRYSTALLIZATION; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MEMBRANE PROTEINS; MICE; PRESENILIN-1;

MUS;

EID: 84868538727     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300997e     Document Type: Article

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