Construction of a stability landscape of the CH3 domain of human IgG1 by combining directed evolution with high throughput sequencing

Journal of Molecular Biology

Volumn 423, Issue 3, 2012, Pages 397-412

  Traxlmayr, Michael W ^a   Hasenhindl, Christoph ^a   Hackl, Matthias ^a   Stadlmayr, Gerhard ^a   Rybka, Jakub D ^a   Borth, Nicole ^a   Grillari, Johannes ^a   Rüker, Florian ^a   Obinger, Christian ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Fc RI binding site; immunoglobulin fold; mutational tolerance; sequence stability relationship; yeast surface display

Indexed keywords

CD64 ANTIGEN; CH3 PROTEIN; IMMUNOGLOBULIN G1; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; COMPUTER MODEL; CRYSTALLIZATION; ENERGY; EXPERIMENTAL STUDY; GENE MUTATION; HIGH THROUGHPUT SEQUENCING; MOLECULAR EVOLUTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE; QUALITATIVE ANALYSIS; WILD TYPE; YEAST;

EID: 84867069553     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2012.07.017     Document Type: Article

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