Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1824, Issue 4, 2012, Pages 542-549

  Traxlmayr, Michael W ^a   Faissner, Maximilian ^a   Stadlmayr, Gerhard ^a   Hasenhindl, Christoph ^a   Antes, Bernhard ^b   Rüker, Florian ^a   Obinger, Christian ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b Babraham Research Campus   (Austria)

Author keywords

Antibody engineering; Crystallizable domain; Directed evolution; Stability engineering; Yeast display

Indexed keywords

CD16 ANTIGEN; FC RECEPTOR; IMMUNOGLOBULIN G1; PLASMID DNA; PROTEIN A;

ARTICLE; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; DNA ISOLATION; FLUORESCENCE ACTIVATED CELL SORTING; GEL PERMEATION CHROMATOGRAPHY; HEAT SHOCK; HEAT STRESS; KINETICS; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; THERMOSTABILITY; YEAST;

CALORIMETRY, DIFFERENTIAL SCANNING; CIRCULAR DICHROISM; DIRECTED MOLECULAR EVOLUTION; HEAT-SHOCK RESPONSE; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MUTATION, MISSENSE; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, IGG; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; THERMODYNAMICS;

EID: 84857263421     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2012.01.006     Document Type: Article

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